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| These transgenic mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the human keratin 14 (KRT14) gene promoter (active in embryonic and postnatal basal cells of the skin). When mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (tetO), expression of the target gene may be regulated by the tetracycline analog, doxycycline (dox); in the presence of dox, transcription of the target gene is induced in cells where rtTA is expressed. This strain provides a Tet-On tool that allows the inducible expression of genes in cells expressing KRT14 for hair and skin research. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
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Description
Strain Information
Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Noncarrier x Hemizygote (Female x Male) 02-MAY-08 Species laboratory mouse Generation ?+N2F11 (27-NOV-11)
Generation DefinitionsDonating Investigator Elaine Fuchs, The Rockefeller University Description
Mice that are hemizygous or homozygous for this transgenic insert are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These transgenic mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the human keratin 14 (KRT14) gene promoter (active in embryonic and postnatal basal cells of the skin). When mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (tetO), expression of the target gene may be regulated by the tetracycline analog, doxycycline (dox); in the presence of dox, transcription of the target gene is induced in cells where rtTA is expressed. This strain provides a Tet-On tool that allows the inducible expression of genes in cells expressing KRT14 for hair and skin research.Development
A transgenic vector incorporating the human keratin 14 promoter/enhancer, rabbit beta globin 5' untranslated region (UTR) for stabilization of transcript, the reverse tetracycline regulatable transactivator protein (rtTA2S-M2-VP16), and the keratin 14 3' UTR was injected into FVB embryos. This strain has been maintained on an FVB background by the donating laboratory.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of KRT14
006403 129S.B6-Tg(KRT14-Esr1/HRAS)1Pkha/J 006661 129S.B6-Tg(KRT14-RAF1/ESR1)1Pkha/J 004853 B6.C3-Tg(KRT14-Birc5)19Gros/J 009687 B6.Cg-Tg(KRT14-Kitl*)4XTG2Bjl/J 007622 B6;SJL-Tg(KRT14-Hr)551Cct/J 007678 B6;SJL-Tg(KRT14-rtTA)208Jek/J 002980 C57BL/6-Tg(Cd152Ig)1Jbs/J 006822 FVB-Tg(KRT14-MAP2K1/Esr1)12Pkha/J 005705 FVB-Tg(KRT14-Vegfa)3Dtm/J 017005 FVB.Cg-Tg(KRT14-Fyn*)aJsey/J 004782 STOCK Tg(KRT14-cre)1Amc/J 005107 STOCK Tg(KRT14-cre/ERT)20Efu/J View Strains carrying other alleles of KRT14 (12 strains)
Additional Web Information
Tet Expression Systems
Phenotype
Phenotype Information
assigned by genotype
Tg(KRT14-rtTA)F42Efu/0
involves: FVB
- normal phenotype
- no abnormal phenotype detected
- hemizygotes are viable, fertile, and normal in size; mice do not display any gross physical or behavioral abnormalities (MGI Ref ID J:116057)
This mouse can be used to support research in many areas including:
Research Tools
Tet Expression Systems
tTA/rtTA Expressing Strains
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(KRT14-rtTA)F42Efu | ||
|---|---|---|---|
| Allele Name | transgene insertion F42, Elaine Fuchs | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | K14rtTA; | ||
| Mutation Made By | Elaine Fuchs, The Rockefeller University | ||
| Strain of Origin | FVB | ||
| Site of Expression | embryonic and postnatal basal cells of the skin | ||
| Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli | ||
| The tetracycline repressor gene (Tetr), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). One mutant with a four amino acid residue change (rTetR) exhibited dependence on tetracycline for induction of the targeted gene and was used in the rtTA construct (Gossen et al, 1995). rTetr was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). | |||
| Promoter | KRT14, keratin 14, human | ||
| Molecular Note | A transgenic vector incorporating the human keratin 14 promoter/enhancer, rabbit beta globin 5' untranslated region (UTR) for stabilization of transcript, the reverse tetracycline regulatable transactivator protein (rtTA2S-M2-VP16), and the keratin 14 3'UTR was injected into FVB embryos. [MGI Ref ID J:116057] [MGI Ref ID J:56822] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(KRT14-rtTA)F42Efu, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Nguyen H; Rendl M; Fuchs E. 2006. Tcf3 governs stem cell features and represses cell fate determination in skin. Cell 127(1):171-83. [PubMed: 17018284] [MGI Ref ID J:116057]
Additional References
Tg(KRT14-rtTA)F42Efu relatedGrachtchouk M; Pero J; Yang SH; Ermilov AN; Michael LE; Wang A; Wilbert D; Patel RM; Ferris J; Diener J; Allen M; Lim S; Syu LJ; Verhaegen M; Dlugosz AA. 2011. Basal cell carcinomas in mice arise from hair follicle stem cells and multiple epithelial progenitor populations. J Clin Invest 121(5):1768-81. [PubMed: 21519145] [MGI Ref ID J:173930]
Jackson SJ; Zhang Z; Feng D; Flagg M; O'Loughlin E; Wang D; Stokes N; Fuchs E; Yi R. 2013. Rapid and widespread suppression of self-renewal by microRNA-203 during epidermal differentiation. Development 140(9):1882-91. [PubMed: 23571213] [MGI Ref ID J:195491]
Lu CP; Polak L; Rocha AS; Pasolli HA; Chen SC; Sharma N; Blanpain C; Fuchs E. 2012. Identification of stem cell populations in sweat glands and ducts reveals roles in homeostasis and wound repair. Cell 150(1):136-50. [PubMed: 22770217] [MGI Ref ID J:186240]
Ousset M; Van Keymeulen A; Bouvencourt G; Sharma N; Achouri Y; Simons BD; Blanpain C. 2012. Multipotent and unipotent progenitors contribute to prostate postnatal development. Nat Cell Biol 14(11):1131-8. [PubMed: 23064263] [MGI Ref ID J:193931]
Van Keymeulen A; Rocha AS; Ousset M; Beck B; Bouvencourt G; Rock J; Sharma N; Dekoninck S; Blanpain C. 2011. Distinct stem cells contribute to mammary gland development and maintenance. Nature 479(7372):189-93. [PubMed: 21983963] [MGI Ref ID J:180048]
Vasioukhin V; Degenstein L; Wise B; Fuchs E. 1999. The magical touch: genome targeting in epidermal stem cells induced by tamoxifen application to mouse skin. Proc Natl Acad Sci U S A 96(15):8551-6. [PubMed: 10411913] [MGI Ref ID J:56822]
Zhang H; Pasolli HA; Fuchs E. 2011. Yes-associated protein (YAP) transcriptional coactivator functions in balancing growth and differentiation in skin. Proc Natl Acad Sci U S A 108(6):2270-5. [PubMed: 21262812] [MGI Ref ID J:169106]
Zhang L; Stokes N; Polak L; Fuchs E. 2011. Specific MicroRNAs are preferentially expressed by skin stem cells to balance self-renewal and early lineage commitment. Cell Stem Cell 8(3):294-308. [PubMed: 21362569] [MGI Ref ID J:171125]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintained as a live colony, hemizygotes may be bred to wildtype siblings or to FVB/NJ inbred mice. Mating System Noncarrier x Hemizygote (Female x Male) 02-MAY-08 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Hemizygous for Tg(KRT14-rtTA)F42Efu
Price per Pair (US dollars $) Pair Genotype $296.00 Hemizygous for Tg(KRT14-rtTA)F42Efu x Noncarrier $296.00 Noncarrier x Hemizygous for Tg(KRT14-rtTA)F42Efu Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Hemizygous for Tg(KRT14-rtTA)F42Efu
Price per Pair (US dollars $) Pair Genotype $384.80 Hemizygous for Tg(KRT14-rtTA)F42Efu x Noncarrier $384.80 Noncarrier x Hemizygous for Tg(KRT14-rtTA)F42Efu Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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|
|
Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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