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| These transgenic mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the human keratin 14 (KRT14) gene promoter (active in embryonic and postnatal basal cells of the skin). When mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (tetO), expression of the target gene may be regulated by the tetracycline analog, doxycycline (dox); in the presence of dox, transcription of the target gene is induced in cells where rtTA is expressed. This strain provides a Tet-On tool that allows the inducible expression of genes in cells expressing KRT14 for hair and skin research. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Noncarrier x Hemizygote (Female x Male) 02-MAY-08 Species laboratory mouse Generation ?+N6 (10-NOV-09) Donating Investigator Elaine Fuchs, The Rockefeller University Description
Mice that are hemizygous or homozygous for this transgenic insert are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These transgenic mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the human keratin 14 (KRT14) gene promoter (active in embryonic and postnatal basal cells of the skin). When mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (tetO), expression of the target gene may be regulated by the tetracycline analog, doxycycline (dox); in the presence of dox, transcription of the target gene is induced in cells where rtTA is expressed. This strain provides a Tet-On tool that allows the inducible expression of genes in cells expressing KRT14 for hair and skin research.Development
A transgenic vector incorporating the human keratin 14 promoter/enhancer, rabbit beta globin 5' untranslated region (UTR) for stabilization of transcript, the reverse tetracycline regulatable transactivator protein (rtTA2S-M2-VP16), and the keratin 14 3' UTR was injected into FVB embryos. This strain has been maintained on an FVB background by the donating laboratory.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of KRT14
006403 129S.B6-Tg(KRT14-Esr1/HRAS)1Pkha/J 006661 129S.B6-Tg(KRT14-RAF1/ESR1)1Pkha/J 004853 B6.C3-Tg(KRT14-Birc5)19Gros/J 007622 B6;SJL-Tg(KRT14-Hr)551Cct/J 007678 B6;SJL-Tg(KRT14-rtTA)208Jek/J 002980 C57BL/6-Tg(Cd152Ig)1Jbs/J 006822 FVB-Tg(KRT14-MAP2K1/Esr1)12Pkha/J 005705 FVB-Tg(KRT14-Vegfa)3Dtm/J 004782 STOCK Tg(KRT14-cre)1Amc/J 005107 STOCK Tg(KRT14-cre/Esr1)20Efu/J View Strains carrying other alleles of KRT14 (10 strains)
006965 B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae/J 005670 B6.Cg-Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J 007176 B6.Cg-Tg(Pax8-rtTA2S*M2)1Koes/J 006235 B6.Cg-Tg(SFTPC-rtTA)5Jaw/J 006232 B6.Cg-Tg(Scgb1a1-rtTA)1Jaw/J 006911 B6;129-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm2(tetO-Pou5f1)Jae/J 007678 B6;SJL-Tg(KRT14-rtTA)208Jek/J 010576 B6;SJL-Tg(MMTV-rtTA)4-1Jek/J 006245 C.Cg-Tg(SFTPC-rtTA)5Jaw/J 006242 C.Cg-Tg(Scgb1a1-rtTA)1Jaw/J 004127 FVB-Tg(Nes-rtTA)306Rvs/J 006225 FVB.Cg-Tg(SFTPC-rtTA)5Jaw/J 006222 FVB.Cg-Tg(Scgb1a1-rtTA)1Jaw/J 008202 FVB/N-Tg(NPHS2-rtTA2*M2)1Jbk/J 006875 FVB/N-Tg(Tagln-rtTA)E1Jwst/J 004602 NOD.Cg-Tg(Ins2-rtTA)2Doi/DoiJ 005734 NOD/Lt-Tg(Ins2-rtTA)1Ach/AchJ 005572 STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J 008755 STOCK Tg(Ins2-rtTA)2Efr Tg(teto-DTA)1Gfi/J 008250 STOCK Tg(Ins2-rtTA)2Efr/J 005493 STOCK Tg(Tek-rtTA,TRE-lacZ)1425Tpr/J 003273 STOCK Tg(rtTAhCMV)4Bjd/J
Additional Web Information
Tet Expression Systems
Phenotype
Phenotype Information
assigned by genotype
Tg(KRT14-rtTA)F42Efu/0
involves: FVB
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:116057)
- hemizygotes are viable, fertile, and normal in size; mice do not display any gross physical or behavioral abnormalities
This mouse can be used to support research in many areas including:
Research Tools
Tet Expression Systems
tTA/rtTA Expressing Strains
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(KRT14-rtTA)F42Efu | ||
|---|---|---|---|
| Allele Name | transgene insertion F42, Elaine Fuchs | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | K14rtTA; | ||
| Mutation Made By | Elaine Fuchs, The Rockefeller University | ||
| Strain of Origin | FVB | ||
| Site of Expression | embryonic and postnatal basal cells of the skin | ||
| Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli | ||
| The tetracycline repressor gene (Tetr), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). One mutant with a four amino acid residue change (rTetR) exhibited dependence on tetracycline for induction of the targeted gene and was used in the rtTA construct (Gossen et al, 1995). rTetr was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). | |||
| Promoter | KRT14, keratin 14, human | ||
| Molecular Note | A transgenic vector incorporating the human keratin 14 promoter/enhancer, rabbit beta globin 5' untranslated region (UTR) for stabilization of transcript, the reverse tetracycline regulatable transactivator protein (rtTA2S-M2-VP16), and the keratin 14 3'UTR was injected into FVB embryos. [MGI Ref ID J:116057] [MGI Ref ID J:56822] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(KRT14-rtTA)F42Efu, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Nguyen H; Rendl M; Fuchs E. 2006. Tcf3 governs stem cell features and represses cell fate determination in skin. Cell 127(1):171-83. [PubMed: 17018284] [MGI Ref ID J:116057]
Additional References
Tg(KRT14-rtTA)F42Efu relatedVasioukhin V; Degenstein L; Wise B; Fuchs E. 1999. The magical touch: genome targeting in epidermal stem cells induced by tamoxifen application to mouse skin. Proc Natl Acad Sci U S A 96(15):8551-6. [PubMed: 10411913] [MGI Ref ID J:56822]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintained as a live colony, hemizygotes may be bred to wildtype siblings or to FVB/NJ inbred mice. Mating System Noncarrier x Hemizygote (Female x Male) 02-MAY-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(KRT14-rtTA)F42Efu
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Tg(KRT14-rtTA)F42Efu x Noncarrier $297.85 Noncarrier x Hemizygous for Tg(KRT14-rtTA)F42Efu
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(KRT14-rtTA)F42Efu
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Tg(KRT14-rtTA)F42Efu x Noncarrier $387.30 Noncarrier x Hemizygous for Tg(KRT14-rtTA)F42Efu
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
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