Strain Name:

FVB-Tg(KRT14-rtTA)F42Efu/J

Stock Number:

008099

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Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
This transgenic strain provides a Tet-On tool that allows doxycycline (dox) inducible expression of genes in cells where the human KRT14 promoter is active and may be useful in applications for hair and skin research.

Description

Strain Information

Type Coisogenic; Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
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Mating SystemNoncarrier x Hemizygote         (Female x Male)   02-MAY-08
Specieslaboratory mouse
Generation?+N2F13 (28-DEC-13)
Generation Definitions
 
Donating Investigator Elaine Fuchs,   The Rockefeller University

Description
Mice that are hemizygous or homozygous for this transgenic insert are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These transgenic mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the human keratin 14 (KRT14) gene promoter (active in embryonic and postnatal basal cells of the skin). When mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (tetO), expression of the target gene may be regulated by the tetracycline analog, doxycycline (dox); in the presence of dox, transcription of the target gene is induced in cells where rtTA is expressed. This strain provides a Tet-On tool that allows the inducible expression of genes in cells expressing KRT14 for hair and skin research.

Development
A transgenic vector incorporating the human keratin 14 promoter/enhancer, rabbit beta globin 5' untranslated region (UTR) for stabilization of transcript, the reverse tetracycline regulatable transactivator protein (rtTA2S-M2-VP16), and the keratin 14 3' UTR was injected into FVB embryos. This strain has been maintained on an FVB background by the donating laboratory.

Control Information

  Control
   Noncarrier
   001800 FVB/NJ
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of KRT14     (13 strains)

Strains carrying other alleles of rtTA
016567   129S.Cg-Tg(Hoxb7-rtTA*M2)2Cos/J
017983   B6.Cg-Col1a1tm9(tetO-Dnmt3b_i1)Jae Gt(ROSA)26Sortm1(rtTA*M2)Jae/J
014588   B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm6(tetO-MSI2)Jae/J
014602   B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm1(tetO-mCherry)Eggn/J
023749   B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae Tg(tetO-Pou5f1,-Sox2,-Klf4,-Myc)1Srn/J
006965   B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae/J
005670   B6.Cg-Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
016997   B6.Cg-Tg(Axin2-rtTA2S*M2)7Cos/J
012418   B6.Cg-Tg(CD68-rtTA2S*M2)3Mpil/Mmjax
014098   B6.Cg-Tg(GFAP-rtTA*M2)1Rmra/J
007176   B6.Cg-Tg(Pax8-rtTA2S*M2)1Koes/J
006235   B6.Cg-Tg(SFTPC-rtTA)5Jaw/J
006232   B6.Cg-Tg(Scgb1a1-rtTA)1Jaw/J
021025   B6;129-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm1(tetO-cre)Haho/J
006911   B6;129-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm2(tetO-Pou5f1)Jae/J
016836   B6;129S4-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm7(tetO-HIST1H2BJ/GFP)Jae/J
012433   B6;C3-Tg(ACTA1-rtTA,tetO-cre)102Monk/J
021187   B6;FVB-Tg(Pbsn-rtTA*M2)42Xy/J
010574   B6;SJL-Tg(Gh1-rtTA)4-3Jek/J
007678   B6;SJL-Tg(KRT14-rtTA)208Jek/J
010576   B6;SJL-Tg(MMTV-rtTA)4-1Jek/J
010549   B6N.Cg-Tg(Prkcd-glc-1-rtTA)2And/J
016532   B6N.FVB(Cg)-Tg(CAG-rtTA3)4288Slowe/J
006245   C.Cg-Tg(SFTPC-rtTA)5Jaw/J
006242   C.Cg-Tg(Scgb1a1-rtTA)1Jaw/J
017955   C57BL/6-Tg(Gfap-rtTA,tetO-MAOB,-lacZ)1Jkan/J
004127   FVB-Tg(Nes-rtTA)306Rvs/J
008326   FVB-Tg(Pomc-rtTA)1Rck/J
006225   FVB.Cg-Tg(SFTPC-rtTA)5Jaw/J
006222   FVB.Cg-Tg(Scgb1a1-rtTA)1Jaw/J
008202   FVB/N-Tg(NPHS2-rtTA2*M2)1Jbk/J
006875   FVB/N-Tg(Tagln-rtTA)E1Jwst/J
004602   NOD.Cg-Tg(Ins2-rtTA)2Doi/DoiJ
005734   NOD/Lt-Tg(Ins2-rtTA)1Ach/AchJ
011004   STOCK Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm3(tetO-Pou5f1,-Sox2,-Klf4,-Myc)Jae/J
011011   STOCK Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm4(tetO-Pou5f1,-Sox2,-Klf4,-Myc)Jae/J
011013   STOCK Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm5(tetO-Pou5f1,-Klf4,-Myc)Jae/J
005572   STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
016116   STOCK Waptm2(rtTA)Kuw/J
003273   STOCK Tg(CMV-rtTA)4Bjd/J
018156   STOCK Tg(Drd1a-rtTA)ARgmk/J
019110   STOCK Tg(Hoxb7-rtTA*M2)RS40BCos/Mmjax
008755   STOCK Tg(Ins2-rtTA)2Efr Tg(teto-DTA)1Gfi/J
008250   STOCK Tg(Ins2-rtTA)2Efr/J
017519   STOCK Tg(KRT5-rtTA)T2D6Sgkd/J
016146   STOCK Tg(SFTPC-rtTA)2Jaw/J
016145   STOCK Tg(Scgb1a1-rtTA)2Jaw/J
005493   STOCK Tg(Tek-rtTA,TRE-lacZ)1425Tpr/J
View Strains carrying other alleles of rtTA     (48 strains)

Additional Web Information

Tet Expression Systems

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Tg(KRT14-rtTA)F42Efu/0

        involves: FVB
  • normal phenotype
  • no abnormal phenotype detected
    • hemizygotes are viable, fertile, and normal in size; mice do not display any gross physical or behavioral abnormalities   (MGI Ref ID J:116057)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Research Tools
Tet Expression Systems
      tTA/rtTA Expressing Strains

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(KRT14-rtTA)F42Efu
Allele Name transgene insertion F42, Elaine Fuchs
Allele Type Transgenic (Inserted expressed sequence)
Common Name(s) K14rtTA;
Mutation Made By Elaine Fuchs,   The Rockefeller University
Strain of OriginFVB
Site of Expressionembryonic and postnatal basal cells of the skin
Expressed Gene rtTA, reverse tetracycline-controlled transactivator, E. coli
The tetracycline repressor gene (Tetr), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). One mutant with a four amino acid residue change (rTetR) exhibited dependence on tetracycline for induction of the targeted gene and was used in the rtTA construct (Gossen et al, 1995). rTetr was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16).
Promoter KRT14, keratin 14, human
Molecular Note A transgenic vector incorporating the human keratin 14 promoter/enhancer, rabbit beta globin 5' untranslated region (UTR) for stabilization of transcript, the reverse tetracycline regulatable transactivator protein (rtTA2S-M2-VP16), and the keratin 14 3'UTR was injected into FVB embryos. [MGI Ref ID J:116057] [MGI Ref ID J:56822]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(KRT14-rtTA)F42Efu, Melt Curve Analysis
Tg(KRT14-rtTA)F42Efu, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Nguyen H; Rendl M; Fuchs E. 2006. Tcf3 governs stem cell features and represses cell fate determination in skin. Cell 127(1):171-83. [PubMed: 17018284]  [MGI Ref ID J:116057]

Additional References

Tg(KRT14-rtTA)F42Efu related

Folgueras AR; Guo X; Pasolli HA; Stokes N; Polak L; Zheng D; Fuchs E. 2013. Architectural niche organization by LHX2 is linked to hair follicle stem cell function. Cell Stem Cell 13(3):314-27. [PubMed: 24012369]  [MGI Ref ID J:202029]

Gay D; Kwon O; Zhang Z; Spata M; Plikus MV; Holler PD; Ito M; Yang Z; Treffeisen E; Kim CD; Nace A; Zhang X; Baratono S; Wang F; Ornitz DM; Millar SE; Cotsarelis G. 2013. Fgf9 from dermal gammadelta T cells induces hair follicle neogenesis after wounding. Nat Med 19(7):916-23. [PubMed: 23727932]  [MGI Ref ID J:199838]

Grachtchouk M; Pero J; Yang SH; Ermilov AN; Michael LE; Wang A; Wilbert D; Patel RM; Ferris J; Diener J; Allen M; Lim S; Syu LJ; Verhaegen M; Dlugosz AA. 2011. Basal cell carcinomas in mice arise from hair follicle stem cells and multiple epithelial progenitor populations. J Clin Invest 121(5):1768-81. [PubMed: 21519145]  [MGI Ref ID J:173930]

Jackson SJ; Zhang Z; Feng D; Flagg M; O'Loughlin E; Wang D; Stokes N; Fuchs E; Yi R. 2013. Rapid and widespread suppression of self-renewal by microRNA-203 during epidermal differentiation. Development 140(9):1882-91. [PubMed: 23571213]  [MGI Ref ID J:195491]

Lee SE; Sada A; Zhang M; McDermitt DJ; Lu SY; Kemphues KJ; Tumbar T. 2014. High Runx1 levels promote a reversible, more-differentiated cell state in hair-follicle stem cells during quiescence. Cell Rep 6(3):499-513. [PubMed: 24462289]  [MGI Ref ID J:208785]

Lien WH; Polak L; Lin M; Lay K; Zheng D; Fuchs E. 2014. In vivo transcriptional governance of hair follicle stem cells by canonical Wnt regulators. Nat Cell Biol 16(2):179-90. [PubMed: 24463605]  [MGI Ref ID J:208262]

Lu CP; Polak L; Rocha AS; Pasolli HA; Chen SC; Sharma N; Blanpain C; Fuchs E. 2012. Identification of stem cell populations in sweat glands and ducts reveals roles in homeostasis and wound repair. Cell 150(1):136-50. [PubMed: 22770217]  [MGI Ref ID J:186240]

Ousset M; Van Keymeulen A; Bouvencourt G; Sharma N; Achouri Y; Simons BD; Blanpain C. 2012. Multipotent and unipotent progenitors contribute to prostate postnatal development. Nat Cell Biol 14(11):1131-8. [PubMed: 23064263]  [MGI Ref ID J:193931]

Van Keymeulen A; Rocha AS; Ousset M; Beck B; Bouvencourt G; Rock J; Sharma N; Dekoninck S; Blanpain C. 2011. Distinct stem cells contribute to mammary gland development and maintenance. Nature 479(7372):189-93. [PubMed: 21983963]  [MGI Ref ID J:180048]

Vasioukhin V; Degenstein L; Wise B; Fuchs E. 1999. The magical touch: genome targeting in epidermal stem cells induced by tamoxifen application to mouse skin. Proc Natl Acad Sci U S A 96(15):8551-6. [PubMed: 10411913]  [MGI Ref ID J:56822]

Zhang H; Pasolli HA; Fuchs E. 2011. Yes-associated protein (YAP) transcriptional coactivator functions in balancing growth and differentiation in skin. Proc Natl Acad Sci U S A 108(6):2270-5. [PubMed: 21262812]  [MGI Ref ID J:169106]

Zhang L; Stokes N; Polak L; Fuchs E. 2011. Specific MicroRNAs are preferentially expressed by skin stem cells to balance self-renewal and early lineage commitment. Cell Stem Cell 8(3):294-308. [PubMed: 21362569]  [MGI Ref ID J:171125]

Zhang Y; Lam O; Nguyen MT; Ng G; Pear WS; Ai W; Wang IJ; Kao WW; Liu CY. 2013. Mastermind-like transcriptional co-activator-mediated Notch signaling is indispensable for maintaining conjunctival epithelial identity. Development 140(3):594-605. [PubMed: 23293291]  [MGI Ref ID J:194073]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX11

Colony Maintenance

Breeding & HusbandryWhen maintained as a live colony, hemizygotes may be bred to wildtype siblings or to FVB/NJ inbred mice.
Mating SystemNoncarrier x Hemizygote         (Female x Male)   02-MAY-08
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $239.00Female or MaleHemizygous for Tg(KRT14-rtTA)F42Efu  
Price per Pair (US dollars $)Pair Genotype
$311.00Hemizygous for Tg(KRT14-rtTA)F42Efu x Noncarrier  
$311.00Noncarrier x Hemizygous for Tg(KRT14-rtTA)F42Efu  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $310.70Female or MaleHemizygous for Tg(KRT14-rtTA)F42Efu  
Price per Pair (US dollars $)Pair Genotype
$404.30Hemizygous for Tg(KRT14-rtTA)F42Efu x Noncarrier  
$404.30Noncarrier x Hemizygous for Tg(KRT14-rtTA)F42Efu  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   Noncarrier
   001800 FVB/NJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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