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Strain Name:

B6.129-Prdm1tm1Clme/J

Stock Number:

008100

Availability:

Repository- Live

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Additional Licensing and Use Restrictions

Genes & Alleles   Prdm1;   Prdm1tm1Clme;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Targeted Mutation
Mating SystemHeterozygote x Heterozygote         (Female x Male)
Specieslaboratory mouse
Donating Investigator Kathryn Calame,   Columbia University
GenerationN10+N1+N0 (21-APR-08)

Strain Description
These mice possess loxP sites in introns flanking exons 6 to 8 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 6 to 8 deleted in the cre-expressing tissue(s).

When bred to a strain expressing Cre recombinase during B-lymphocyte development and differentiation (see Stock No. 004126 for example), this mutant mouse strain may be useful in studies of humoral immune response.

Strain Development
A targeting vector containing a loxP site flanked neomycin selection cassette and a herpes simplex virus thymidine kinase gene was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 8 of the targeted gene, and another loxP site was inserted downstream of exon 5. This construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a Cre expression plasmid for the purpose of removing the selectable marker cassette. ES cells that had successfully undergone Cre recombination and no longer retained the cassette but did retain the loxP-flanked exons 6-8 were injected in C57BL/6J blastocysts. Resulting chimeric animals were backcrossed to C57BL/6J for 10 generations before arriving at The Jackson Laboratory.

Mammalian Phenotype Terms assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

Cd19tm1(cre)Cgn/Cd19+ Prdm1tm1Clme/Prdm1tm1Clme

        involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ   (conditional)
  • immune system phenotype
  • abnormal B cell differentiation (MGI Ref ID J:101890)
    • following immunization a severe decrease in the number of antigen-specific IgM secreting cells is seen compared to wild type mice
    • a larger increase in CD138-B220+NP+ B cells expressing GL7 is seen at 7 and 14 days after immunization
    • abnormal plasma cell differentiation (MGI Ref ID J:101890)
      • the development of both short-lived and post-germinal center long-lived plasma cells is blocked in mutants
  • abnormal memory B cell number (MGI Ref ID J:101890)
    • pre-plasma memory B-cells are reduced by 75% in the spleen and 95% in the bone marrow, and CD138-B220+NP+ cells comprise the majority of memory B cells
  • abnormal plasma cell morphology (MGI Ref ID J:101890)
    • abnormal plasma cell differentiation (MGI Ref ID J:101890)
      • the development of both short-lived and post-germinal center long-lived plasma cells is blocked in mutants
    • decreased plasma cell number (MGI Ref ID J:101890)
      • very few CD138+B220+/- plasma cells are seen at any time before or after immunization
      • 5 days after re-challenge a 99% reduction in CD138+ B220+/- NP+ plasma cells is seen
  • decreased immunoglobulin level (MGI Ref ID J:101890)
    • serum Ig levels are reduced in unimmunized mice
    • decreased IgG level (MGI Ref ID J:101890)
      • in mice immunized with NP-Ficoll serum IgG1 and IgG2a levels are significantly lower in mutants compared to wild type mice and mutant mice fail to secrete Ig in a recall response
    • decreased IgM level (MGI Ref ID J:101890)
      • in mice immunized with NP-Ficoll serum IgM levels are significantly lower in mutants compared to wild type mice and homozygous mice fail to secrete Ig in a recall response
  • increased spleen germinal center number (MGI Ref ID J:101890)
    • more numerous germinal centers are seen after immunization
  • increased spleen germinal center size (MGI Ref ID J:101890)
    • larger germinal centers are seen after immunization
  • hematopoietic system phenotype
  • abnormal B cell differentiation (MGI Ref ID J:101890)
    • following immunization a severe decrease in the number of antigen-specific IgM secreting cells is seen compared to wild type mice
    • a larger increase in CD138-B220+NP+ B cells expressing GL7 is seen at 7 and 14 days after immunization
    • abnormal plasma cell differentiation (MGI Ref ID J:101890)
      • the development of both short-lived and post-germinal center long-lived plasma cells is blocked in mutants
  • abnormal memory B cell number (MGI Ref ID J:101890)
    • pre-plasma memory B-cells are reduced by 75% in the spleen and 95% in the bone marrow, and CD138-B220+NP+ cells comprise the majority of memory B cells
  • abnormal plasma cell morphology (MGI Ref ID J:101890)
    • abnormal plasma cell differentiation (MGI Ref ID J:101890)
      • the development of both short-lived and post-germinal center long-lived plasma cells is blocked in mutants
    • decreased plasma cell number (MGI Ref ID J:101890)
      • very few CD138+B220+/- plasma cells are seen at any time before or after immunization
      • 5 days after re-challenge a 99% reduction in CD138+ B220+/- NP+ plasma cells is seen
  • increased spleen germinal center number (MGI Ref ID J:101890)
    • more numerous germinal centers are seen after immunization
  • increased spleen germinal center size (MGI Ref ID J:101890)
    • larger germinal centers are seen after immunization

Gene & Allele Details

Allele Symbol Prdm1tm1Clme
Allele Name targeted mutation 1, Kathryn Calame
Common Name(s) Prdm1flox;
Mutation Made By Kathryn Calame,   Columbia University
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line NameR1
ES Cell Line Strain(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name Prdm1, PR domain containing 1, with ZNF domain
Chromosome 10
Gene Common Name(s) B lymphocyte induced maturation protein; BLIMP1; Blimp-1; Blimp1; MGC118922; MGC118923; MGC118924; MGC118925; PRDI-BF1;
Molecular Note LoxP sites were inserted to flank exons 6-8, encompassing sequence that encodes the zinc finger motifs. [MGI Ref ID J:101890]

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Genotyping Protocols

Prdm1tm1Clme

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as homozygotes.
Diet Information LabDiet® 5K52/5K67

Additional Web Information

Congenic Nomenclature
Cre-lox or FLP-FRT Systems

Animal Health Reports

Room Number           AX11

Research Applications

This mouse can be used to support research in many areas including:

Research Tools
Cre-lox System (loxP-flanked Sequences)
Developmental Biology Research (Cre-lox System)

References

Selected Reference(s)

Shapiro-Shelef M; Lin KI; McHeyzer-Williams LJ; Liao J; McHeyzer-Williams MG; Calame K. 2003. Blimp-1 is required for the formation of immunoglobulin secreting plasma cells and pre-plasma memory B cells. Immunity 19(4):607-20. [PubMed: 14563324]  [MGI Ref ID J:101890]

Additional References

Price and Supply Information

Strain Name: B6.129-Prdm1tm1Clme/J
Stock Number: 008100

Price Details

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Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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