Strain Name:

B6.129-Prdm1tm1Clme/J

Stock Number:

008100

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Availability:

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Use Restrictions Apply, see Terms of Use
These Prdm1 floxed conditional mutant mice may be useful in generating conditional mutations to study humoral immune response, plasma memory B-cells, and B-lymphocyte development and differentiation.

Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Mating SystemHomozygote x Homozygote         (Female x Male)   06-JAN-09
Specieslaboratory mouse
GenerationN11+N1F13 (30-APR-13)
Generation Definitions
 
Donating Investigator Kathryn Calame,   Columbia University

Description
These mice possess loxP sites in introns flanking exons 6 to 8 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 6 to 8 deleted in the cre-expressing tissue(s).

When bred to a strain expressing Cre recombinase during B-lymphocyte development and differentiation (see Stock No. 004126 for example), this mutant mouse strain may be useful in studies of humoral immune response.

When bred to mice carrying Tg(Itgax-cre)1-1Reiz (Stock No. 008068), Cre recombinase expression in dendritic cells results in altered adaptive immune response and lupus-like serology.

Development
A targeting vector containing a loxP site flanked neomycin selection cassette and a herpes simplex virus thymidine kinase gene was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 8 of the targeted gene, and another loxP site was inserted downstream of exon 5. This construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a Cre expression plasmid for the purpose of removing the selectable marker cassette. ES cells that had successfully undergone Cre recombination and no longer retained the cassette but did retain the loxP-flanked exons 6-8 were injected in C57BL/6J blastocysts. Resulting chimeric animals were backcrossed to C57BL/6J for 10 generations before arriving at The Jackson Laboratory.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Prdm1
008828   B6.Cg-Tg(Prdm1-EYFP)1Mnz/J
008827   B6.Cg-Tg(Prdm1-cre)1Masu/J
View Strains carrying other alleles of Prdm1     (2 strains)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Model with phenotypic similarity to human disease where etiologies are distinct. Human genes are associated with this disease. Orthologs of these genes do not appear in the mouse genotype(s).
Systemic Lupus Erythematosus; SLE
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

Cd19tm1(cre)Cgn/Cd19+ Prdm1tm1Clme/Prdm1tm1Clme

        involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ   (conditional)
  • immune system phenotype
  • abnormal B cell differentiation
    • following immunization a severe decrease in the number of antigen-specific IgM secreting cells is seen compared to wild-type mice   (MGI Ref ID J:101890)
    • a larger increase in CD138-B220+NP+ B cells expressing GL7 is seen at 7 and 14 days after immunization   (MGI Ref ID J:101890)
    • abnormal plasma cell differentiation
      • the development of both short-lived and post-germinal center long-lived plasma cells is blocked in mutants   (MGI Ref ID J:101890)
  • abnormal memory B cell number
    • pre-plasma memory B-cells are reduced by 75% in the spleen and 95% in the bone marrow, and CD138-B220+NP+ cells comprise the majority of memory B cells   (MGI Ref ID J:101890)
  • abnormal plasma cell morphology   (MGI Ref ID J:101890)
    • antibody stimulating cell development is completely inhibited in cultures of stimulated B cells, however stimulation of B cells with LPS or LPS and IL-4 results in increased class switch recombination   (MGI Ref ID J:114881)
    • abnormal plasma cell differentiation
      • the development of both short-lived and post-germinal center long-lived plasma cells is blocked in mutants   (MGI Ref ID J:101890)
    • decreased plasma cell number
      • very few CD138+B220+/- plasma cells are seen at any time before or after immunization   (MGI Ref ID J:101890)
      • 5 days after re-challenge a 99% reduction in CD138+ B220+/- NP+ plasma cells is seen   (MGI Ref ID J:101890)
  • decreased immunoglobulin level
    • serum Ig levels are reduced in unimmunized mice   (MGI Ref ID J:101890)
    • decreased IgG level
      • in mice immunized with NP-Ficoll serum IgG1 and IgG2a levels are significantly lower in mutants compared to wild-type mice and mutant mice fail to secrete Ig in a recall response   (MGI Ref ID J:101890)
    • decreased IgM level
      • in mice immunized with NP-Ficoll serum IgM levels are significantly lower in mutants compared to wild-type mice and homozygous mice fail to secrete Ig in a recall response   (MGI Ref ID J:101890)
  • increased spleen germinal center number
    • more numerous germinal centers are seen after immunization   (MGI Ref ID J:101890)
  • increased spleen germinal center size
    • larger germinal centers are seen after immunization   (MGI Ref ID J:101890)
  • hematopoietic system phenotype
  • abnormal B cell differentiation
    • following immunization a severe decrease in the number of antigen-specific IgM secreting cells is seen compared to wild-type mice   (MGI Ref ID J:101890)
    • a larger increase in CD138-B220+NP+ B cells expressing GL7 is seen at 7 and 14 days after immunization   (MGI Ref ID J:101890)
    • abnormal plasma cell differentiation
      • the development of both short-lived and post-germinal center long-lived plasma cells is blocked in mutants   (MGI Ref ID J:101890)
  • abnormal memory B cell number
    • pre-plasma memory B-cells are reduced by 75% in the spleen and 95% in the bone marrow, and CD138-B220+NP+ cells comprise the majority of memory B cells   (MGI Ref ID J:101890)
  • abnormal plasma cell morphology   (MGI Ref ID J:101890)
    • antibody stimulating cell development is completely inhibited in cultures of stimulated B cells, however stimulation of B cells with LPS or LPS and IL-4 results in increased class switch recombination   (MGI Ref ID J:114881)
    • abnormal plasma cell differentiation
      • the development of both short-lived and post-germinal center long-lived plasma cells is blocked in mutants   (MGI Ref ID J:101890)
    • decreased plasma cell number
      • very few CD138+B220+/- plasma cells are seen at any time before or after immunization   (MGI Ref ID J:101890)
      • 5 days after re-challenge a 99% reduction in CD138+ B220+/- NP+ plasma cells is seen   (MGI Ref ID J:101890)
  • decreased immunoglobulin level
    • serum Ig levels are reduced in unimmunized mice   (MGI Ref ID J:101890)
    • decreased IgG level
      • in mice immunized with NP-Ficoll serum IgG1 and IgG2a levels are significantly lower in mutants compared to wild-type mice and mutant mice fail to secrete Ig in a recall response   (MGI Ref ID J:101890)
    • decreased IgM level
      • in mice immunized with NP-Ficoll serum IgM levels are significantly lower in mutants compared to wild-type mice and homozygous mice fail to secrete Ig in a recall response   (MGI Ref ID J:101890)
  • increased spleen germinal center number
    • more numerous germinal centers are seen after immunization   (MGI Ref ID J:101890)
  • increased spleen germinal center size
    • larger germinal centers are seen after immunization   (MGI Ref ID J:101890)

Prdm1tm1Clme/Prdm1tm1Clme Tg(Itgax-cre)1-1Reiz/0

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA   (conditional)
  • immune system phenotype
  • abnormal T follicular helper cell differentiation
    • generation of follicular T helper cells cells is enhanced when mutant dendritic cells are cultured with naive T cells from control mice in the presence of differentiation factors, indicating increased differentiation of these cells   (MGI Ref ID J:178876)
  • abnormal immune system physiology
    • enhanced immune response after NP-CGG immunization with an increased high-affinity anti-NP IgG response, an increase in the number of germinal center B cells, and an increase in the number of total germinal cells and antigen-specific germinal cells   (MGI Ref ID J:178876)
    • abnormal CD4-positive T cell physiology
      • increase in expression of ICOS in CD4+ T cells, indicating that mice have more activated CD4+ T cells than controls   (MGI Ref ID J:178876)
    • abnormal germinal center B cell physiology
      • enhanced germinal center response in young mice   (MGI Ref ID J:178876)
    • increased autoantibody level
      • females, but not males, develop autoantibodies as early as 4-5 months of age, including anti-nuclear antibodies, anti-double stranded dNA, and anti-ENA5, which are all IgG   (MGI Ref ID J:178876)
      • increased anti-nuclear antigen antibody level   (MGI Ref ID J:178876)
        • increased anti-double stranded DNA antibody level
          • IgG2b is the major isotype of anti-dsDNA antibodies   (MGI Ref ID J:178876)
    • increased immunoglobulin level
      • total serum immunoglobulin levels are increased in females but not males   (MGI Ref ID J:178876)
      • increased IgG level
        • kidney deposition of IgG in 10 month old females   (MGI Ref ID J:178876)
    • increased interleukin-6 secretion
      • increase in production of IL-6 by splenic dendritic cells and by bone marrow derived dendritic cells after LPS stimulation in females but not males   (MGI Ref ID J:178876)
    • kidney inflammation
      • inflammatory infiltrates in 10 month old females   (MGI Ref ID J:178876)
  • increased T follicular helper cell number
    • increase in the percentage and number of follicular T helper cells   (MGI Ref ID J:178876)
  • increased germinal center B cell number
    • more spontaneous germinal center B cells   (MGI Ref ID J:178876)
  • increased spleen germinal center number
    • more germinal centers in the spleen   (MGI Ref ID J:178876)
  • increased splenocyte number
    • total number of splenocytes are increased in females but not males   (MGI Ref ID J:178876)
  • renal/urinary system phenotype
  • abnormal kidney morphology
    • kidney deposition of IgG in 10 month old females   (MGI Ref ID J:178876)
    • abnormal mesangial cell morphology
      • mesangial cell proliferation in 10 month old females   (MGI Ref ID J:178876)
  • increased urine protein level
    • proteinuria in 10 month old females   (MGI Ref ID J:178876)
  • kidney inflammation
    • inflammatory infiltrates in 10 month old females   (MGI Ref ID J:178876)
  • hematopoietic system phenotype
  • abnormal CD4-positive T cell physiology
    • increase in expression of ICOS in CD4+ T cells, indicating that mice have more activated CD4+ T cells than controls   (MGI Ref ID J:178876)
  • abnormal T follicular helper cell differentiation
    • generation of follicular T helper cells cells is enhanced when mutant dendritic cells are cultured with naive T cells from control mice in the presence of differentiation factors, indicating increased differentiation of these cells   (MGI Ref ID J:178876)
  • abnormal germinal center B cell physiology
    • enhanced germinal center response in young mice   (MGI Ref ID J:178876)
  • increased T follicular helper cell number
    • increase in the percentage and number of follicular T helper cells   (MGI Ref ID J:178876)
  • increased germinal center B cell number
    • more spontaneous germinal center B cells   (MGI Ref ID J:178876)
  • increased immunoglobulin level
    • total serum immunoglobulin levels are increased in females but not males   (MGI Ref ID J:178876)
    • increased IgG level
      • kidney deposition of IgG in 10 month old females   (MGI Ref ID J:178876)
  • increased spleen germinal center number
    • more germinal centers in the spleen   (MGI Ref ID J:178876)
  • increased splenocyte number
    • total number of splenocytes are increased in females but not males   (MGI Ref ID J:178876)
  • homeostasis/metabolism phenotype
  • increased urine protein level
    • proteinuria in 10 month old females   (MGI Ref ID J:178876)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Research Tools
Cre-lox System
      loxP-flanked Sequences
Developmental Biology Research
      Cre-lox System

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Prdm1tm1Clme
Allele Name targeted mutation 1, Kathryn Calame
Allele Type Targeted (Floxed/Frt)
Common Name(s) Blimp-1flox; Prdm1flox;
Mutation Made By Kathryn Calame,   Columbia University
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line NameR1
ES Cell Line Strain(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name Prdm1, PR domain containing 1, with ZNF domain
Chromosome 10
Gene Common Name(s) B lymphocyte induced maturation protein; BLIMP1; Blimp-1; Blimp1; PRDI-BF1;
Molecular Note LoxP sites were inserted to flank exons 6-8, encompassing sequence that encodes the zinc finger motifs. [MGI Ref ID J:101890]

Genotyping

Genotyping Information

Genotyping Protocols

Prdm1tm1Clme, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Shapiro-Shelef M; Lin KI; McHeyzer-Williams LJ; Liao J; McHeyzer-Williams MG; Calame K. 2003. Blimp-1 is required for the formation of immunoglobulin secreting plasma cells and pre-plasma memory B cells. Immunity 19(4):607-20. [PubMed: 14563324]  [MGI Ref ID J:101890]

Additional References

Prdm1tm1Clme related

Brzezinski JA 4th; Lamba DA; Reh TA. 2010. Blimp1 controls photoreceptor versus bipolar cell fate choice during retinal development. Development 137(4):619-29. [PubMed: 20110327]  [MGI Ref ID J:156665]

Brzezinski JA 4th; Uoon Park K; Reh TA. 2013. Blimp1 (Prdm1) prevents re-specification of photoreceptors into retinal bipolar cells by restricting competence. Dev Biol 384(2):194-204. [PubMed: 24125957]  [MGI Ref ID J:204690]

Chan YH; Chiang MF; Tsai YC; Su ST; Chen MH; Hou MS; Lin KI. 2009. Absence of the transcriptional repressor Blimp-1 in hematopoietic lineages reveals its role in dendritic cell homeostatic development and function. J Immunol 183(11):7039-46. [PubMed: 19915049]  [MGI Ref ID J:157392]

Chiang MF; Yang SY; Lin IY; Hong JB; Lin SJ; Ying HY; Chen CM; Wu SY; Liu FT; Lin KI. 2013. Inducible deletion of the Blimp-1 gene in adult epidermis causes granulocyte-dominated chronic skin inflammation in mice. Proc Natl Acad Sci U S A 110(16):6476-81. [PubMed: 23576729]  [MGI Ref ID J:196147]

Cimmino L; Martins GA; Liao J; Magnusdottir E; Grunig G; Perez RK; Calame KL. 2008. Blimp-1 attenuates Th1 differentiation by repression of ifng, tbx21, and bcl6 gene expression. J Immunol 181(4):2338-47. [PubMed: 18684923]  [MGI Ref ID J:140193]

Gururajan M; Simmons A; Dasu T; Spear BT; Calulot C; Robertson DA; Wiest DL; Monroe JG; Bondada S. 2008. Early growth response genes regulate B cell development, proliferation, and immune response. J Immunol 181(7):4590-602. [PubMed: 18802061]  [MGI Ref ID J:141295]

Harper J; Mould A; Andrews RM; Bikoff EK; Robertson EJ. 2011. The transcriptional repressor Blimp1/Prdm1 regulates postnatal reprogramming of intestinal enterocytes. Proc Natl Acad Sci U S A 108(26):10585-90. [PubMed: 21670299]  [MGI Ref ID J:173543]

Horsley V; O'Carroll D; Tooze R; Ohinata Y; Saitou M; Obukhanych T; Nussenzweig M; Tarakhovsky A; Fuchs E. 2006. Blimp1 defines a progenitor population that governs cellular input to the sebaceous gland. Cell 126(3):597-609. [PubMed: 16901790]  [MGI Ref ID J:115995]

Iwasaki Y; Fujio K; Okamura T; Yanai A; Sumitomo S; Shoda H; Tamura T; Yoshida H; Charnay P; Yamamoto K. 2013. Egr-2 transcription factor is required for Blimp-1-mediated IL-10 production in IL-27-stimulated CD4(+) T cells. Eur J Immunol 43(4):1063-73. [PubMed: 23349024]  [MGI Ref ID J:195056]

Johnston RJ; Choi YS; Diamond JA; Yang JA; Crotty S. 2012. STAT5 is a potent negative regulator of TFH cell differentiation. J Exp Med 209(2):243-50. [PubMed: 22271576]  [MGI Ref ID J:181874]

Katoh K; Omori Y; Onishi A; Sato S; Kondo M; Furukawa T. 2010. Blimp1 suppresses Chx10 expression in differentiating retinal photoreceptor precursors to ensure proper photoreceptor development. J Neurosci 30(19):6515-26. [PubMed: 20463215]  [MGI Ref ID J:160529]

Kim SJ; Gregersen PK; Diamond B. 2013. Regulation of dendritic cell activation by microRNA let-7c and BLIMP1. J Clin Invest :. [PubMed: 23298838]  [MGI Ref ID J:194496]

Kim SJ; Zou YR; Goldstein J; Reizis B; Diamond B. 2011. Tolerogenic function of Blimp-1 in dendritic cells. J Exp Med 208(11):2193-9. [PubMed: 21948081]  [MGI Ref ID J:178876]

Lin MH; Chou FC; Yeh LT; Fu SH; Chiou HY; Lin KI; Chang DM; Sytwu HK. 2013. B lymphocyte-induced maturation protein 1 (BLIMP-1) attenuates autoimmune diabetes in NOD mice by suppressing Th1 and Th17 cells. Diabetologia 56(1):136-46. [PubMed: 23052053]  [MGI Ref ID J:194560]

Lord CA; Savitsky D; Sitcheran R; Calame K; Wright JR; Ting JP; Williams KL. 2009. Blimp-1/PRDM1 mediates transcriptional suppression of the NLR gene NLRP12/Monarch-1. J Immunol 182(5):2948-58. [PubMed: 19234190]  [MGI Ref ID J:146247]

Magnusdottir E; Kalachikov S; Mizukoshi K; Savitsky D; Ishida-Yamamoto A; Panteleyev AA; Calame K. 2007. Epidermal terminal differentiation depends on B lymphocyte-induced maturation protein-1. Proc Natl Acad Sci U S A 104(38):14988-93. [PubMed: 17846422]  [MGI Ref ID J:125198]

Martins GA; Cimmino L; Liao J; Magnusdottir E; Calame K. 2008. Blimp-1 directly represses Il2 and the Il2 activator Fos, attenuating T cell proliferation and survival. J Exp Med 205(9):1959-65. [PubMed: 18725523]  [MGI Ref ID J:138810]

Martins GA; Cimmino L; Shapiro-Shelef M; Szabolcs M; Herron A; Magnusdottir E; Calame K. 2006. Transcriptional repressor Blimp-1 regulates T cell homeostasis and function. Nat Immunol 7(5):457-65. [PubMed: 16565721]  [MGI Ref ID J:112556]

Muto A; Ochiai K; Kimura Y; Itoh-Nakadai A; Calame KL; Ikebe D; Tashiro S; Igarashi K. 2010. Bach2 represses plasma cell gene regulatory network in B cells to promote antibody class switch. EMBO J 29(23):4048-61. [PubMed: 20953163]  [MGI Ref ID J:167182]

Nakamura A; Ebina-Shibuya R; Itoh-Nakadai A; Muto A; Shima H; Saigusa D; Aoki J; Ebina M; Nukiwa T; Igarashi K. 2013. Transcription repressor Bach2 is required for pulmonary surfactant homeostasis and alveolar macrophage function. J Exp Med 210(11):2191-204. [PubMed: 24127487]  [MGI Ref ID J:206538]

Nishikawa K; Nakashima T; Hayashi M; Fukunaga T; Kato S; Kodama T; Takahashi S; Calame K; Takayanagi H. 2010. Blimp1-mediated repression of negative regulators is required for osteoclast differentiation. Proc Natl Acad Sci U S A 107(7):3117-22. [PubMed: 20133620]  [MGI Ref ID J:157572]

Omori SA; Cato MH; Anzelon-Mills A; Puri KD; Shapiro-Shelef M; Calame K; Rickert RC. 2006. Regulation of class-switch recombination and plasma cell differentiation by phosphatidylinositol 3-kinase signaling. Immunity 25(4):545-57. [PubMed: 17000121]  [MGI Ref ID J:114881]

Pan H; O'Brien TF; Zhang P; Zhong XP. 2012. The role of tuberous sclerosis complex 1 in regulating innate immunity. J Immunol 188(8):3658-66. [PubMed: 22412198]  [MGI Ref ID J:184070]

Pelletier N; McHeyzer-Williams LJ; Wong KA; Urich E; Fazilleau N; McHeyzer-Williams MG. 2010. Plasma cells negatively regulate the follicular helper T cell program. Nat Immunol 11(12):1110-8. [PubMed: 21037578]  [MGI Ref ID J:167325]

Robertson EJ; Charatsi I; Joyner CJ; Koonce CH; Morgan M; Islam A; Paterson C; Lejsek E; Arnold SJ; Kallies A; Nutt SL; Bikoff EK. 2007. Blimp1 regulates development of the posterior forelimb, caudal pharyngeal arches, heart and sensory vibrissae in mice. Development 134(24):4335-45. [PubMed: 18039967]  [MGI Ref ID J:129204]

Salehi S; Bankoti R; Benevides L; Willen J; Couse M; Silva JS; Dhall D; Meffre E; Targan S; Martins GA. 2012. B Lymphocyte-Induced Maturation Protein-1 Contributes to Intestinal Mucosa Homeostasis by Limiting the Number of IL-17-Producing CD4+ T Cells. J Immunol 189(12):5682-93. [PubMed: 23162130]  [MGI Ref ID J:190850]

Savitsky D; Calame K. 2006. B-1 B lymphocytes require Blimp-1 for immunoglobulin secretion. J Exp Med 203(10):2305-14. [PubMed: 16954370]  [MGI Ref ID J:124626]

Shapiro-Shelef M; Lin KI; Savitsky D; Liao J; Calame K. 2005. Blimp-1 is required for maintenance of long-lived plasma cells in the bone marrow. J Exp Med 202(11):1471-6. [PubMed: 16314438]  [MGI Ref ID J:118853]

Sun J; Dodd H; Moser EK; Sharma R; Braciale TJ. 2011. CD4(+) T cell help and innate-derived IL-27 induce Blimp-1-dependent IL-10 production by antiviral CTLs. Nat Immunol 12(4):327-34. [PubMed: 21297642]  [MGI Ref ID J:170347]

Tsao HW; Tai TS; Tseng W; Chang HH; Grenningloh R; Miaw SC; Ho IC. 2013. Ets-1 facilitates nuclear entry of NFAT proteins and their recruitment to the IL-2 promoter. Proc Natl Acad Sci U S A 110(39):15776-81. [PubMed: 24019486]  [MGI Ref ID J:201146]

Vincent SD; Dunn NR; Sciammas R; Shapiro-Shalef M; Davis MM; Calame K; Bikoff EK; Robertson EJ. 2005. The zinc finger transcriptional repressor Blimp1/Prdm1 is dispensable for early axis formation but is required for specification of primordial germ cells in the mouse. Development 132(6):1315-25. [PubMed: 15750184]  [MGI Ref ID J:101718]

Vincent SD; Mayeuf A; Niro C; Saitou M; Buckingham M. 2012. Non Conservation of Function for the Evolutionarily Conserved Prdm1 Protein in the Control of the Slow Twitch Myogenic Program in the Mouse Embryo. Mol Biol Evol :. [PubMed: 22522309]  [MGI Ref ID J:186268]

Yang Z; Sullivan BM; Allen CD. 2012. Fluorescent in vivo detection reveals that IgE(+) B cells are restrained by an intrinsic cell fate predisposition. Immunity 36(5):857-72. [PubMed: 22406270]  [MGI Ref ID J:187336]

Yoon HS; Scharer CD; Majumder P; Davis CW; Butler R; Zinzow-Kramer W; Skountzou I; Koutsonanos DG; Ahmed R; Boss JM. 2012. ZBTB32 is an early repressor of the CIITA and MHC class II gene expression during B cell differentiation to plasma cells. J Immunol 189(5):2393-403. [PubMed: 22851713]  [MGI Ref ID J:189853]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           FGB27

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as homozygotes.
Mating SystemHomozygote x Homozygote         (Female x Male)   06-JAN-09
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHomozygous for Prdm1tm1Clme  
Price per Pair (US dollars $)Pair Genotype
$464.00Homozygous for Prdm1tm1Clme x Homozygous for Prdm1tm1Clme  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHomozygous for Prdm1tm1Clme  
Price per Pair (US dollars $)Pair Genotype
$603.20Homozygous for Prdm1tm1Clme x Homozygous for Prdm1tm1Clme  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

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phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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