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| These mutant mice have an amino acid substitution that inactivates the cyclooxygenase activity but not the peroxidase activity of the gene product. Mutant mice exhibit abnormal kidney development and function as well as elevated systolic blood pressure, which may make them useful in studies of thrombogenesis and hypertension. | |||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6.129S6-Ptgs2tm1.1Fun/J (Changed: 30-NOV-07 ) Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Heterozygote x Heterozygote (Female x Male) Species laboratory mouse Generation N11+ (18-JUL-08) Donating Investigator Colin Funk, Queen's University Description
Mice that are homozygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. Female homozygotes are not fertile. The protein gene product does not have cyclooxygenase activity while the peroxidase activity is normal as measured by LPS induction of macrophages derived from mutant mice. Mutant mice are more sensitive to collagen treatment resulting in reduction of platelet numbers (indicating enhanced thrombogenesis) and exhibit a higher frequency of sudden death due to thromboxane receptor agonist treatment. No prolonged bleeding time from LPS administration is observed. Prostaglandin E metabolite levels are diminished in urine. Three month old mutant mice have elevated systolic blood pressure (measured by the tail cuff method). Homozygotes exhibit undersize, pale kidneys with atrophic cortices, interstitial inflammation, hypoplastic glomeruli and glomerulosclerosis. This mutant mouse strain may be useful in studies of thrombogenesis and hypertension.Development
A targeting vector containing a loxP site flanked neomycin resistance gene and a point mutation was used to insert a Y385F substitution into exon 8. The construct was electroporated into 129S6/SvEvTac derived TL1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The mice were crossed to the Cre recombinase expressing strain FVB/N-Tg(EIIa-cre)C5379Lmgd/J (Stock No. 003314) for two generations to remove the neo selection cassette, and then backcrossed to C57BL/6 for 10 generations.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Ptgs2
008104 B6.129(FVB)-Ptgs2tm2.1(Ptgs1)Fun/J 002476 B6;129S-Ptgs2tm1Jed/J View Strains carrying other alleles of Ptgs2 (2 strains)
Additional Web Information
Congenic Nomenclature
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Ptgs2tm1.1Fun/Ptgs2tm1.1Fun
involves: 129S6/SvEvTac * C57BL/6
- renal/urinary system phenotype
- abnormal kidney cortex (MGI Ref ID J:109548)
- atrophic
- abnormal renal glomerulus morphology (MGI Ref ID J:109548)
- hypoplastic
- glomerulosclerosis (MGI Ref ID J:109548)
- moderate
- pale kidney (MGI Ref ID J:109548)
- small kidney (MGI Ref ID J:109548)
- reproductive system phenotype
- reduced female fertility (MGI Ref ID J:109548)
- number of term pregnancies reduced
- cardiovascular system phenotype
- abnormal PGE2 physiology (MGI Ref ID J:109548)
- near absence of LPS induced synthesis
This mouse can be used to support research in many areas including:
Cardiovascular Research
Hypertension
Vascular Defects (Thrombosis)
Developmental Biology Research
Internal/Organ Defects (kidney)
Internal/Organ Research
Kidney Defects
Reproductive Biology Research
Fertility Defects (females only)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Ptgs2tm1.1Fun | ||
|---|---|---|---|
| Allele Name | targeted mutation 1.1, Colin D Funk | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | Ptgs2Y385F; | ||
| Strain of Origin | 129S6/SvEvTac | ||
| ES Cell Line Name | TL1/TL-1 | ||
| ES Cell Line Strain | 129S6/SvEvTac | ||
| Gene Symbol and Name | Ptgs2, prostaglandin-endoperoxide synthase 2 | ||
| Chromosome | 1 | ||
| Gene Common Name(s) | COX-2; COX2; GRIPGHS; PGG/HS; PGHS-2; PHS-2; Pghs2; Tis10; cyclooxygenase 2; cyclooxygenase-2; hCox-2; prostaglandin G/H synthase; | ||
| Molecular Note | This allele was generated by crossing mice carrying Ptgs2tm1Fun/sup> to mice with the Tg(EIIa-cre)C5379Lmgd transgene. The resulted in the loss of the neomycin selection cassette. [MGI Ref ID J:109548] | ||
Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Yu Y; Fan J; Chen XS; Wang D; Klein-Szanto AJ; Campbell RL; Fitzgerald GA; Funk CD. 2006. Genetic model of selective COX2 inhibition reveals novel heterodimer signaling. Nat Med 12(6):699-704. [PubMed: 16732282] [MGI Ref ID J:109548]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony of these mice, heterozygotes may be interbred or heterozygous females may be crossed with homozygous males. Homozygous females are not fertile. Mating System Heterozygote x Heterozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $236.40 Female or Male Heterozygous for Ptgs2tm1.1Fun *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $472.80 Heterozygous for Ptgs2tm1.1Fun x Heterozygous for Ptgs2tm1.1Fun
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $307.40 Female or Male Heterozygous for Ptgs2tm1.1Fun *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $614.70 Heterozygous for Ptgs2tm1.1Fun x Heterozygous for Ptgs2tm1.1Fun
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Terms of Use
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General Terms and Conditions
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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