Strain Name: |
B6.Cg-Tg(CAG-Ub*G76V/GFP)1Dant/J |
|---|---|
Stock Number: |
008111 |
Availability: | Under Development for Distribution Colony |
| To register your interest in this strain go to the Strain Interest Form. |
General Terms and Conditions |
| Former Name |
B6.Cg-Tg(ACTB-Ub*G76V/GFP)1Dant/J (Changed: 08-MAY-08
) |
| Genes & Alleles | ACTB; GFP; Tg(CAG-Ub*G76V/GFP)1Dant; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Noncarrier x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Nico Dantuma, Karolinska Institutet Generation N31+ (21-JUL-08) Strain Description
Hemizygous transgenic mice are viable and fertile; they contain a green fluorescent protein (GFP) fused to a constitutively active degradation signal (UbG76V). Transgenic transcripts are detected in all tissues examined; however the G76V substitution leads to its ubiquitination and proteasomal degradation, rather than expression of the GFP fluorescent protein. Following administration of proteasome inhibitors, UbG76V accumulates and GFP-derived fluorescence is readily apparent, evidence of an impaired ubiquitin/proteasome system. Both of the ubiquitin/proteasome system reporter founder lines, UbG76V-GFP/1 (Stock No. 008111) and UbG76V-GFP/2 (Stock No. 008112), may be useful for monitoring the role of ubiquitin/proteasome-dependent proteolysis in diverse disorders, and in efficacy trials for monitoring the effect of compounds on the ubiquitin/proteasome system (for example, in tests with proteasome inhibitors as novel anticancer drugs). While this strain and Stock No. 008112 have similar expression patterns, the donating investigator reports that this high expressing line (UbG76V-GFP/1; Stock No. 008111) has very low, but detectable, background fluorescence in the brain, spinal cord, and exocrine pancreatic tissue, prior to administration of proteasome inhibitors. Investigators are urged to consider which of these two founder lines is best suited for their research, because this reported fluorescence may hinder the detection of subtle changes in reporter levels in some tissues.Strain Development
The UbG76V-GFP transgene was designed with a chicken beta-actin promoter (and cytomegalovirus (CMV) immediate early enhancer) upstream of a green fluorescent protein (GFP) fused to a mutant ubiquitin moiety (UbG76V), a constitutively active proteasome degradation signal. The transgene was microinjected into the pronucleus of fertilized (CBAxC57BL/6)F1 oocytes, and the transgenic offspring bred to C57BL/6N. Founder line 1 (UbG76V-GFP/1) mice, with high expression of the transgene, were backcrossed to C57BL/6N for approximately 30 generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred with C57BL/6NJ (Stock No. 005304) to establish the colony.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(CAG-Ub*G76V/GFP)1Dant | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Nico Dantuma | ||
| Common Name(s) | Tg(ACTB-Ub*G76V/GFP)1Dant; Tg(CAG-Ub*G76V/GFP)1Dant; UPS reporter; UbG76V-GFP/2; UbG76V-GFP/1; | ||
| Mutation Made By | Nico Dantuma, Karolinska Institutet | ||
| Strain of Origin | (CBA x C57BL/6)F1 | ||
| Site of Expression | Transgene transcripts are detected in all tissues examined; however the G76V substitution leads to degradation, of the GFP. Following administration of proteasome inhibitors, UbG76V accumulates and GFP-derived fluorescence is apparent. This strain is a high expressing line and has very low, but detectable, background fluorescence in the brain, spinal cord, and exocrine pancreatic tissue, prior to administration of proteasome inhibitors. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | ACTB, actin, beta, chicken | ||
| Molecular Note | The UbG76V-GFP transgene was designed with a chicken beta-actin promoter (and cytomegalovirus (CMV) immediate early enhancer) upstream of a green fluorescent protein (GFP) fused to a mutant ubiquitin moiety (UbG76V). Although transcripts of the UbG76V-GFP fusion gene are detected in all tissues examined, the G76V substitution prevents removal of the ubiquitin moiety leading to efficient ubiquitination and proteasomal degradation of the fusion protein (and thus no fluorescence) in these tissues. Line 1 with high expression was maintained by backcrosses to C57BL/6N mice. The high expressing line (UbG76V-GFP/1) has very low, but detectable, fluorescence expression in brain, spinal cord, and exocrine pancreatic tissue prior to administration of proteasome inhibitors. [MGI Ref ID J:127085] [MGI Ref ID J:127087] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 005304 C57BL/6NJ | (approximate) | |
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tg(CAG-Ub*G76V/GFP)1Dant, Tg(CAG-Ub*G76V/GFP)2Dant
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, hemizygotes may be bred to wildtype siblings or to C57BL/6NJ (Stock No. 005304) inbred mice. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of ACTB
View Strains carrying other alleles of ACTB (31 strains)
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Research Applications
This mouse can be used to support research in many areas including:
GFP relatedCancer Research
Other
Cell Biology Research
Post-translational Processing
Protein Processing (degradation)
Neurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Cancer Research
Cell Biology Research
Fluorescent Proteins
Toxicology Research (drug/compound testing)
Research Tools
Fluorescent Proteins
References
Selected Reference(s)
Additional ReferencesBowman AB; Yoo SY; Dantuma NP; Zoghbi HY. 2005. Neuronal dysfunction in a polyglutamine disease model occurs in the absence of ubiquitin-proteasome system impairment and inversely correlates with the degree of nuclear inclusion formation. Hum Mol Genet 14(5):679-91. [PubMed: 15661755] [MGI Ref ID J:96393]
Lindsten K; Menendez-Benito V; Masucci MG; Dantuma NP. 2003. A transgenic mouse model of the ubiquitin/proteasome system. Nat Biotechnol 21(8):897-902. [PubMed: 12872133] [MGI Ref ID J:127085]
Price and Supply Information
| Strain Name: | B6.Cg-Tg(CAG-Ub*G76V/GFP)1Dant/J |
| Stock Number: | 008111 |
This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date: 06-OCT-08
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
View All Strains Under Development
Supply Details
| Standard Supply | Under Development for Distribution Colony |
|---|---|
| Supply Notes |
This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
For additional Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form