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| Somatostatin deficient mice exhibit increased baseline plasma growth hormone, corticosterone and total ghrelin levels and impaired motor performance. Hippocampal amyloid beta 42 peptides levels are elevated. This mutant mouse strain may be useful in studies of gastric homeostasis, learning and memory and endocrinology. | |||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Heterozygote x Heterozygote (Female x Male) Species laboratory mouse Generation N11+ (27-AUG-08) Donating Investigator Ute Hochgeschwender, Oklahoma Medical Research Foundation Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA or protein) is detected by Northern blot, in situ hybridization or radioimmunoassay analysis of brain tissue. Homozygotes exhibit increased baseline plasma growth hormone, corticosterone and total ghrelin levels compared to wildtype. Mutant mice have impaired motor performance ability. Somatostatin-deficient mice have enlarged stomachs with an increased number of parietal cells and hyperchlorhydria. Hippocampal neprilysin activity is diminished. Compared to wildtype controls, amyloid beta 42 peptides levels are elevated in the hippocampus. This mutant mouse strain may be useful in studies of gastric homeostasis, learning and memory and endocrinology.Development
A targeting vector containing the neomycin resistance gene was used to disrupt the last 30 base pairs of exon 1 and the first 39 base pairs of intron 1. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells, and correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting male chimeric animals were crossed to 129S6/SvEvTac females, and then backcrossed to C57BL/6NHsd for 10 generations before arriving at The Jackson Laboratory. The mice were then backcrossed to C57BL/6NJ (Stock No. 005304) for one generation during importation.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Ssttm1Ute allele
003117 129S-Ssttm1Ute/J View Strains carrying Ssttm1Ute (1 strain)
Additional Web Information
Congenic Nomenclature
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Ssttm1Ute/Ssttm1Ute
involves: 129S4/SvJae * C57BL/6
- digestive/alimentary phenotype
- abnormal gastric gland (MGI Ref ID J:97527)
- abnormal gastric parietal cell morphology (MGI Ref ID J:97527)
- increased numbers of parietal cells
- enlarged stomach (MGI Ref ID J:97527)
- thickened fundus but no tumors
- stomachs heavier than in controls
- hyperchlorhydria (MGI Ref ID J:97527)
- hyperacidic
This mouse can be used to support research in many areas including:
Ssttm1Ute relatedEndocrine Deficiency Research
Gastrointestinal Defects
Internal/Organ Research
Gastrointestinal Defects
Neurobiology Research
Behavioral and Learning Defects
Cancer Research
Genes Regulating Growth and Proliferation
Endocrine Deficiency Research
Hypothalamus/Pituitary Defects
Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
Inflammation
Neurobiology Research
Ataxia (Movement) Defects
Behavioral and Learning Defects
Cerebellar Defects
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Ssttm1Ute | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Ute Hochgeschwender | ||
| Allele Type | Targeted (knock-out) | ||
| Common Name(s) | Srif-; Sst KO; Sst-; | ||
| Mutation Made By | Ute Hochgeschwender, Oklahoma Medical Research Foundation | ||
| Strain of Origin | 129S4/SvJae | ||
| ES Cell Line Name | J1 | ||
| ES Cell Line Strain | 129S4/SvJae | ||
| Gene Symbol and Name | Sst, somatostatin | ||
| Chromosome | 16 | ||
| Gene Common Name(s) | SMST; SOM; SRIF; SS-14; SS-28; Smst; preprosomatostatin; | ||
| Molecular Note | An SV40-tk-neo cassette was inserted, thereby deleting the last 30 bp of exon 1 and the first 39 basepairs of inton 1. Successful recombination was shown by Southern blot. Northern blot indicated lack of transcript and radioimmunoassay demonstrated thelack of protein from homozygous mutants. [MGI Ref ID J:70426] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Ssttm1Ute, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Zeyda T; Diehl N; Paylor R; Brennan MB; Hochgeschwender U. 2001. Impairment in motor learning of somatostatin null mutant mice. Brain Res 906(1-2):107-14. [PubMed: 11430867] [MGI Ref ID J:70426]
Additional References
Ssttm1Ute relatedLaz EV; Holloway MG; Chen CS; Waxman DJ. 2007. Characterization of three growth hormone-responsive transcription factors preferentially expressed in adult female liver. Endocrinology 148(7):3327-37. [PubMed: 17412818] [MGI Ref ID J:129617]
Luque RM; Gahete MD; Hochgeschwender U; Kineman RD. 2006. Evidence that endogenous SST inhibits ACTH and ghrelin expression by independent pathways. Am J Physiol Endocrinol Metab 291(2):E395-403. [PubMed: 16825606] [MGI Ref ID J:127795]
Saito T; Iwata N; Tsubuki S; Takaki Y; Takano J; Huang SM; Suemoto T; Higuchi M; Saido TC. 2005. Somatostatin regulates brain amyloid beta peptide Abeta(42) through modulation of proteolytic degradation. Nat Med 11(4):434-9. [PubMed: 15778722] [MGI Ref ID J:97539]
Videau C; Hochgeschwender U; Kreienkamp HJ; Brennan MB; Viollet C; Richter D; Epelbaum J. 2003. Characterisation of [125I]-Tyr0DTrp8-somatostatin binding in sst1- to sst4- and SRIF-gene-invalidated mouse brain. Naunyn Schmiedebergs Arch Pharmacol 367(6):562-71. [PubMed: 12759718] [MGI Ref ID J:95635]
Zavros Y; Eaton KA; Kang W; Rathinavelu S; Katukuri V; Kao JY; Samuelson LC; Merchant JL. 2005. Chronic gastritis in the hypochlorhydric gastrin-deficient mouse progresses to adenocarcinoma. Oncogene 24(14):2354-66. [PubMed: 15735748] [MGI Ref ID J:97527]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as homozygotes. Mating System Heterozygote x Heterozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date: 01-DEC-08
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
View All Strains Under Development and On Hold
Supply Details
| Standard Supply | Under Development for Distribution Colony |
|---|---|
| Supply Notes |
|
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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