Strain Name:

B6N.129S4(129S6)-Ssttm1Ute/J

Stock Number:

008117

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Somatostatin deficient mice exhibit increased baseline plasma growth hormone, corticosterone and total ghrelin levels and impaired motor performance. Hippocampal amyloid beta 42 peptides levels are elevated. This mutant mouse strain may be useful in studies of gastric homeostasis, learning and memory and endocrinology.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6.129S4(129S6)-Ssttm1Ute/J    (Changed: 06-NOV-13 )
Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Specieslaboratory mouse
GenerationN1pN1
Generation Definitions
 
Donating InvestigatorDr. Ute Hochgeschwender,   Duke Institute for Brain Science

Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA or protein) is detected by Northern blot, in situ hybridization or radioimmunoassay analysis of brain tissue. Homozygotes exhibit increased baseline plasma growth hormone, corticosterone and total ghrelin levels compared to wildtype. Mutant mice have impaired motor performance ability. Somatostatin-deficient mice have enlarged stomachs with an increased number of parietal cells and hyperchlorhydria. Hippocampal neprilysin activity is diminished. Compared to wildtype controls, amyloid beta 42 peptides levels are elevated in the hippocampus. This mutant mouse strain may be useful in studies of gastric homeostasis, learning and memory and endocrinology.

Development
A targeting vector containing the neomycin resistance gene was used to disrupt the last 30 base pairs of exon 1 and the first 39 base pairs of intron 1. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells, and correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting male chimeric animals were crossed to 129S6/SvEvTac females, and then backcrossed to C57BL/6NHsd for 10 generations before arriving at The Jackson Laboratory. The mice were then backcrossed to C57BL/6NJ (Stock No. 005304) for one generation during importation.

Control Information

  Control
   005304 C57BL/6NJ
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Ssttm1Ute allele
003117   129S-Ssttm1Ute/J
View Strains carrying   Ssttm1Ute     (1 strain)

Strains carrying other alleles of Sst
010708   B6(Cg)-Ssttm1(cre/ERT2)Zjh/J
016868   B6.Cg-Ssttm1.2(tTA2)Hze/J
018973   B6N.Cg-Ssttm2.1(cre)Zjh/J
013044   STOCK Ssttm2.1(cre)Zjh/J
View Strains carrying other alleles of Sst     (4 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Ssttm1Ute/Ssttm1Ute

        involves: 129S4/SvJae * C57BL/6
  • digestive/alimentary phenotype
  • abnormal gastric gland morphology   (MGI Ref ID J:97527)
    • abnormal gastric parietal cell morphology
      • increased numbers of parietal cells   (MGI Ref ID J:97527)
  • abnormal stomach fundus morphology
    • thickened fundus   (MGI Ref ID J:97527)
  • enlarged stomach
    • stomachs heavier than in controls   (MGI Ref ID J:97527)
  • hyperchlorhydria
    • hyperacidic   (MGI Ref ID J:97527)

Ssttm1Ute/Ssttm1Ute

        129-Ssttm1Ute/J
  • behavior/neurological phenotype
  • *normal* behavior/neurological phenotype
    • no overt behavioral abnormalities, including reflexes, anxiety related behaviors, sensorimotor responses, fear conditioning and exploratory activity   (MGI Ref ID J:70426)
    • abnormal motor learning
      • motor learning performance is impaired on the accelerating rotarod test; mutant mice perform similarly to controls in the first two training sessions, but do not improve from trial 2 to 3 as do control mice   (MGI Ref ID J:70426)
      • mutant mice perform similar to controls in coordination, strength and balance tests such as vertical pole and hanging wire test   (MGI Ref ID J:70426)
  • homeostasis/metabolism phenotype
  • *normal* homeostasis/metabolism phenotype
    • T4 plasma levels are similar to controls   (MGI Ref ID J:70426)
    • plasma glucose levels are similar to controls   (MGI Ref ID J:70426)
    • increased circulating corticosterone level
      • plasma corticosterone levels are significantly elevated compared to controls   (MGI Ref ID J:70426)
    • increased circulating growth hormone level
      • plasma growth hormone levels are 3 times greater than controls but body weight is normal   (MGI Ref ID J:70426)
  • nervous system phenotype
  • *normal* nervous system phenotype
    • no gross abnormalities of brain morphology, including the characteristic layering of cells in the cerebellar cortex   (MGI Ref ID J:70426)

Ssttm1Ute/Ssttm1Ute

        involves: 129S4/SvJae * C57BL/6J
  • homeostasis/metabolism phenotype
  • *normal* homeostasis/metabolism phenotype
    • mice exhibit normal insulin sensitivity   (MGI Ref ID J:179095)
    • decreased circulating prolactin level
      • in female and male mice   (MGI Ref ID J:179095)
    • increased circulating ghrelin level
      • female and male mice exhibit increased plasma total ghrelin levels compared with wild-type mice   (MGI Ref ID J:179095)
      • however, acylated ghrelin levels are normal   (MGI Ref ID J:179095)
    • increased circulating insulin level
      • in male, but not female, mice   (MGI Ref ID J:179095)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Endocrine Deficiency Research
Gastrointestinal Defects

Internal/Organ Research
Gastrointestinal Defects

Neurobiology Research
Behavioral and Learning Defects

Ssttm1Ute related

Cancer Research
Genes Regulating Growth and Proliferation

Endocrine Deficiency Research
Hypothalamus/Pituitary Defects

Immunology, Inflammation and Autoimmunity Research
Growth Factors/Receptors/Cytokines
Inflammation

Neurobiology Research
Ataxia (Movement) Defects
Behavioral and Learning Defects
Cerebellar Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Ssttm1Ute
Allele Name targeted mutation 1, Ute Hochgeschwender
Allele Type Targeted (Null/Knockout)
Common Name(s) Srif-; Sst KO; Sst-;
Mutation Made ByDr. Ute Hochgeschwender,   Duke Institute for Brain Science
Strain of Origin129S4/SvJae
ES Cell Line NameJ1
ES Cell Line Strain129S4/SvJae
Gene Symbol and Name Sst, somatostatin
Chromosome 16
Gene Common Name(s) SMST; SOM; SRIF; SS-14; SS-28; Smst; preprosomatostatin;
Molecular Note An SV40-tk-neo cassette was inserted, thereby deleting the last 30 bp of exon 1 and the first 39 basepairs of inton 1. Successful recombination was shown by Southern blot. Northern blot indicated lack of transcript and radioimmunoassay demonstrated thelack of protein from homozygous mutants. [MGI Ref ID J:70426]

Genotyping

Genotyping Information

Genotyping Protocols

Ssttm1Ute, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Zeyda T; Diehl N; Paylor R; Brennan MB; Hochgeschwender U. 2001. Impairment in motor learning of somatostatin null mutant mice. Brain Res 906(1-2):107-14. [PubMed: 11430867]  [MGI Ref ID J:70426]

Additional References

Ssttm1Ute related

Cordoba-Chacon J; Gahete MD; Castano JP; Kineman RD; Luque RM. 2011. Somatostatin and its receptors contribute in a tissue-specific manner to the sex-dependent metabolic (fed/fasting) control of growth hormone axis in mice. Am J Physiol Endocrinol Metab 300(1):E46-54. [PubMed: 20943754]  [MGI Ref ID J:172331]

Cordoba-Chacon J; Gahete MD; Pozo-Salas AI; Martinez-Fuentes AJ; de Lecea L; Gracia-Navarro F; Kineman RD; Castano JP; Luque RM. 2011. Cortistatin is not a somatostatin analogue but stimulates prolactin release and inhibits GH and ACTH in a gender-dependent fashion: potential role of ghrelin. Endocrinology 152(12):4800-12. [PubMed: 21971153]  [MGI Ref ID J:179095]

Laz EV; Holloway MG; Chen CS; Waxman DJ. 2007. Characterization of three growth hormone-responsive transcription factors preferentially expressed in adult female liver. Endocrinology 148(7):3327-37. [PubMed: 17412818]  [MGI Ref ID J:129617]

Luque RM; Gahete MD; Hochgeschwender U; Kineman RD. 2006. Evidence that endogenous SST inhibits ACTH and ghrelin expression by independent pathways. Am J Physiol Endocrinol Metab 291(2):E395-403. [PubMed: 16825606]  [MGI Ref ID J:127795]

Luque RM; Soares BS; Peng XD; Krishnan S; Cordoba-Chacon J; Frohman LA; Kineman RD. 2009. Use of the metallothionein promoter-human growth hormone-releasing hormone (GHRH) mouse to identify regulatory pathways that suppress pituitary somatotrope hyperplasia and adenoma formation due to GHRH-receptor hyperactivation. Endocrinology 150(7):3177-85. [PubMed: 19342460]  [MGI Ref ID J:158164]

Meyer RD; Laz EV; Su T; Waxman DJ. 2009. Male-specific hepatic Bcl6: growth hormone-induced block of transcription elongation in females and binding to target genes inversely coordinated with STAT5. Mol Endocrinol 23(11):1914-26. [PubMed: 19797429]  [MGI Ref ID J:153887]

Saito T; Iwata N; Tsubuki S; Takaki Y; Takano J; Huang SM; Suemoto T; Higuchi M; Saido TC. 2005. Somatostatin regulates brain amyloid beta peptide Abeta(42) through modulation of proteolytic degradation. Nat Med 11(4):434-9. [PubMed: 15778722]  [MGI Ref ID J:97539]

Videau C; Hochgeschwender U; Kreienkamp HJ; Brennan MB; Viollet C; Richter D; Epelbaum J. 2003. Characterisation of [125I]-Tyr0DTrp8-somatostatin binding in sst1- to sst4- and SRIF-gene-invalidated mouse brain. Naunyn Schmiedebergs Arch Pharmacol 367(6):562-71. [PubMed: 12759718]  [MGI Ref ID J:95635]

Zavros Y; Eaton KA; Kang W; Rathinavelu S; Katukuri V; Kao JY; Samuelson LC; Merchant JL. 2005. Chronic gastritis in the hypochlorhydric gastrin-deficient mouse progresses to adenocarcinoma. Oncogene 24(14):2354-66. [PubMed: 15735748]  [MGI Ref ID J:97527]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice may be bred together.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   005304 C57BL/6NJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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