Strain Name: |
B6.129S4-Timp2tm1Pds/J |
|---|---|
Stock Number: |
008120 |
Availability: | Under Development for Distribution Colony |
| To register your interest in this strain go to the Strain Interest Form. |
General Terms and Conditions |
| Genes & Alleles | Timp2; Timp2tm1Pds; |
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Donating Investigator Paul Soloway, Cornell University Strain Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA) is detected by Northern blot analysis of lung tissue. Mutant mice are unable to proteolytically activate proMMP-2, proenzyme matrix metalloproteinase-2. Male homozygotes exhibit deficits in fear-potentiated startle respnses and both male and female mutants exhibit reduced prepulse inhibition. Homozygotes exhibit muscle weakness (due to reduced mass of extensor digitorum longus fast-twitch muscle), decreased motor function, abnormal gait and reduced hindlimb extension. The brains of homozygotes in the postnatal week are smaller in size than wildtype controls, with the size difference disappearing after postnatal day 7. Histological analysis reveals abnormal neuromuscular junctions characterized by a larger size with increased nerve branching, reduced cerebellar cortex thickness, and reduced Purkinje cell processes. Homozygotes have impaired neuronal differentiation. Female homozygotes are passive when handled, while male homozygotes are aggressive. This mutant mouse strain may be useful in studies of extracellular matrix homeostasis, synaptic plasticity in behavior, learning and memory, neuronal differentiation and development of neuromuscular junctions.Strain Development
A targeting vector containing PGKneo cassette was used to disrupt exon 1 and 5' flanking sequence. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to generate homozygous mice. The mice were then backcrossed to C57BL/6 J for 12 generations before arriving at The Jackson Laboratory.
Mammalian Phenotype Terms assigned by genotype |
| Allele Symbol | Timp2tm1Pds | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Paul D Soloway | ||
| Common Name(s) | TIMP-2KO; TIMP2KO; Timp2-; | ||
| Mutation Made By | Paul Soloway, Cornell University | ||
| Strain of Origin | 129S4/SvJae | ||
| ES Cell Line Name | J1 | ||
| ES Cell Line Strain | 129S4/SvJae | ||
| Gene Symbol and Name | Timp2, tissue inhibitor of metalloproteinase 2 | ||
| Chromosome | 11 | ||
| Gene Common Name(s) | CSC-21K; D11Bwg1104e; DNA segment, Chr 11, Brigham & Women's Genetics 1104 expressed; MGC105282; TIMP-2; Timp-2; | ||
| Molecular Note | Replacement of the first coding exon with a neomycin cassette. [MGI Ref ID J:64001] | ||
| Breeding & Husbandry | When maintaining a live colony, these mice can be bred as homozygotes. |
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Congenic Nomenclature
Cell Biology Research
Defects in Extracellular Matrix Molecules
Developmental Biology Research
Defects in Extracellular Matrix Molecules
Neurodevelopmental Defects
Neurobiology Research
Ataxia (Movement) Defects
Behavioral and Learning Defects
Cerebellar Defects (Purkinje cell defect)
Neurodevelopmental Defects
Neuromuscular Defects
Selected Reference(s)
Additional ReferencesWang Z; Juttermann R; Soloway PD. 2000. TIMP-2 is required for efficient activation of proMMP-2 in vivo J Biol Chem 275(34):26411-5. [PubMed: 10827175] [MGI Ref ID J:64001]
| Strain Name: | B6.129S4-Timp2tm1Pds/J |
| Stock Number: | 008120 |
This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date:
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
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| Standard Supply | Under Development for Distribution Colony |
|---|---|
| Supply Notes |
This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below |
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