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| When these RIP-CreER mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in pancreatic beta cells. This mutant mouse strain may be useful in studying pancreatic development, diabetes and obesity. | |||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered Mutant Mice. Mating System Noncarrier x Hemizygote (Female x Male) Species laboratory mouse Generation F?+ (19-AUG-08) Donating Investigator Douglas Melton, Harvard University Description
These RIP-CreER transgenic mice have a tamoxifen inducible Cre-mediated recombination system driven by the rat insulin 2, Ins2, promoter. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain. The mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/Esr1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Tamoxifen administration induces Cre recombination in islet cells of the pancreas. Hemizygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make this strain homozygous.Development
A transgenic construct containing sequence encoding the fusion gene, cre/Esr1, under the control of the rat insulin 2, Ins2, promoter, was introduced into B6CBAF1 donor eggs. Founder lines were subsequently established. The transgenic mice were then crossed to outbred CD-1 mice.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Esr1
006403 129S.B6-Tg(KRT14-Esr1/HRAS)1Pkha/J 004744 B6.129P2-Esr1tm1Ksk/J 004682 B6.Cg-Tg(CAG-cre/Esr1)5Amc/J 005657 B6.Cg-Tg(Myh6-cre/Esr1)1Jmk/J 005650 B6129-Tg(Myh6-cre/Esr1)1Jmk/J 006774 FVB-Tg(Col2a1-cre/ESR1)KA3Smac/J 006822 FVB-Tg(KRT14-MAP2K1/Esr1)12Pkha/J 008464 STOCK Foxa2tm2.1(cre/Esr1)Moon/J 004453 STOCK Tg(CAG-cre/Esr1)5Amc/J 005107 STOCK Tg(KRT14-cre/Esr1)20Efu/J 008119 STOCK Tg(Neurog3-cre/Esr1)1Dam/J View Strains carrying other alleles of Esr1 (11 strains)
Additional Web Information
Cre-lox Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System (Cre-Recombinase Expression: Inducible)
Developmental Biology Research (Cre-lox System)
Diabetes and Obesity Research
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Ins2-cre/Esr1)1Dam | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Douglas A Melton | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | RIP-CreER; | ||
| Mutation Made By | Charles Murtaugh, Harvard University | ||
| Site of Expression | tamoxifin-induced expression in islet cells of the pancreas | ||
| Expressed Gene | Esr1, estrogen receptor 1 (alpha), mouse, laboratory | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Ins2, insulin 2, rat | ||
| Molecular Note | The rat insulin promoter was used to drive expression of tamoxifen-dependent cre recombinase. [MGI Ref ID J:124161] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Neurog3-cre/Esr1)1Dam, Tg(Ins2-cre/Esr1)1Dam, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Dor Y; Brown J; Martinez OI; Melton DA. 2004. Adult pancreatic beta-cells are formed by self-duplication rather than stem-cell differentiation. Nature 429(6987):41-6. [PubMed: 15129273] [MGI Ref ID J:124161]
Additional References
Tg(Ins2-cre/Esr1)1Dam relatedBrennand K; Huangfu D; Melton D. 2007. All beta Cells Contribute Equally to Islet Growth and Maintenance. PLoS Biol 5(7):e163. [PubMed: 17535113] [MGI Ref ID J:124045]
Nir T; Melton DA; Dor Y. 2007. Recovery from diabetes in mice by beta cell regeneration. J Clin Invest 117(9):2553-61. [PubMed: 17786244] [MGI Ref ID J:127412]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as hemizygotes. The Donating Investigator has not attempted to make this strain homozygous. Mating System Noncarrier x Hemizygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $236.40 Female or Male Hemizygous for Tg(Ins2-cre/Esr1)1Dam *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $288.65 Hemizygous for Tg(Ins2-cre/Esr1)1Dam x Noncarrier $288.65 Noncarrier x Hemizygous for Tg(Ins2-cre/Esr1)1Dam
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $307.40 Female or Male Hemizygous for Tg(Ins2-cre/Esr1)1Dam *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $375.30 Hemizygous for Tg(Ins2-cre/Esr1)1Dam x Noncarrier $375.30 Noncarrier x Hemizygous for Tg(Ins2-cre/Esr1)1Dam
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Tel: 800.422.6423 or 207.288.5845
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Terms of Use
Terms of Use
General Terms and Conditions
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Notice Regarding cre/Esr1 (Tamoxifen Inducible Cre) Strains.
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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