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| These CD4-GFP transgenic mice may be useful for fluorescent monitoring of T cells both in vivo and in vitro. | |||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Mating System Noncarrier x Hemizygote (Female x Male) Species laboratory mouse Generation [F4N1+F3p]+N0 (27-DEC-07) Donating Investigator IMR Colony, The Jackson Laboratory Description
The donating investigator reports that mice homozygous for this CD4-GFP transgene are viable, fertile, normal in size and do not exhibit any gross physical or behavioral abnormalities. FACS analysis of splenic lymphocytes shows transgenic expression uniformly in 81% of inactivated or resting CD8+, 81% on inactivated or resting CD4+, and minimal expression in B cells (2.3%) and macrophages (1.5%). Antigen activated CD4+ T cells continue to express GFP, while activated CD8+ T cells lost GFP expression. These CD4-GFP transgenic mice may be useful for fluorescent monitoring of T cells both in vivo and in vitro.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described on a NOD/ShiLt genetic background. We will modify the strain description if necessary as published results become available.
Development
A transgenic construct containing the coding region of the enhanced green florescent protein, EGFP, gene under the control of the murine Cd4 promoter and enhancer was injected into fertilized NOD/ShiLt mouse eggs. Founder line 1 was mated to NOD/ShiLt prior to sibling mating. These mice were sent to the Type 1 Diabetes Repository (T1DR) at The Jackson Laboratory in 2007 (as Stock No. 005334). Upon arrival, some mice were additionally sent to The Induced Mutant Resource at The Jackson Laboratory and then backcrossed to C57BL/6J to generate this congenic strain (Stock No. 008126).
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (168 strains)
005334 NOD/ShiLt-Tg(Cd4-EGFP)1Lt/J
005551 B6.Cg-Tg(Cd4-TGFBR2)16Flv/J 004687 C.Cg-Tg(SKP2)4Paga/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
GFP relatedResearch Tools
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers)
Genetics Research (Tissue/Cell Markers: T cell specific surface marker)
Genetics Research (Tissue/Cell Markers: transplantation marker for embryonic and adult tissue)
Immunology and Inflammation Research (T cell specific surface marker)
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Cd4-EGFP)1Lt | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Edward Leiter | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | Cd4-GFP line 1; Cd4-GFP line1; | ||
| Mutation Made By | Edward Leiter, The Jackson Laboratory | ||
| Strain of Origin | NOD/ShiLt | ||
| Site of Expression | FACs analysis has detected expression of the transgene in 81% of inactivated or resting CD8+ T cells and CD4+ T cells, with minimal expression in B cells (2.3%) and macrophages (1.5%). | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Cd4, Cd4 molecule, rat | ||
| Molecular Note | A construct containing an enhanced green florescent protein cDNA fused to the murine Cd4 promoter and enhancer was injected into NOD/ShiLt fertilized embryos. T-cells glow green. 80% of T-cells show GFP expression by FACS while B-cells and macrophages donot express the transgene. [MGI Ref ID J:123949] [MGI Ref ID J:123950] | ||
Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Reifsnyder P; Leiter E. 2007. GFP expression in CD8+ T-cells of NOD/ShiLt transgenic mice MGI Direct Data Submission :. [MGI Ref ID J:123950]
Additional References
Tg(Cd4-EGFP)1Lt relatedManjunath N; Shankar P; Stockton B; Dubey PD; Lieberman J; von Andrian UH. 1999. A transgenic mouse model to analyze CD8(+) effector T cell differentiation in vivo. Proc Natl Acad Sci U S A 96(24):13932-7. [PubMed: 10570176] [MGI Ref ID J:123949]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry Mutant mice were bred to C57BL/6J mice to generate this congenic strain. When maintaining the live congenic colony, hemizygous mice are bred with wildtype siblings or to C57BL/6J inbred mice. Mating System Noncarrier x Hemizygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $236.40 Female or Male Hemizygous for Tg(Cd4-EGFP)1Lt *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $288.65 Hemizygous for Tg(Cd4-EGFP)1Lt x Noncarrier $288.65 Noncarrier x Hemizygous for Tg(Cd4-EGFP)1Lt
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $307.40 Female or Male Hemizygous for Tg(Cd4-EGFP)1Lt *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $375.30 Hemizygous for Tg(Cd4-EGFP)1Lt x Noncarrier $375.30 Noncarrier x Hemizygous for Tg(Cd4-EGFP)1Lt
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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