| ||||||||||||||||
| Hemizygous transgenic mice are viable and fertile and develop a Parkinson-like phenotype upon aging. Hind limb paralysis due to loss of motor neurons and a resting tremor are initially seen at about six months of age. No Lewy body-like pathology is noted. Cell death in the spinal cord (extensive) and brain are observed. | ||||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6.Cg-Tg(THY1-SNCA*A53T)1Sud/J (Changed: 22-JAN-08 ) Type Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Noncarrier x Hemizygote (Female x Male) 26-JUL-08 Species laboratory mouse Generation N5+F4 (18-NOV-09)
Generation DefinitionsDonating Investigator Thomas Sudhof, Stanford University School of Medicine Description
Hemizygous transgenic mice are viable and fertile and develop a Parkinson-like phenotype upon aging. Hind limb paralysis due to loss of motor neurons and a resting tremor are initially seen at about six months of age. No Lewy body-like pathology is noted. Cell death in the spinal cord (extensive) and brain are observed.Development
This transgenic strain carries the human SNCA (synuclein, alpha) gene under the control of a human THY1 (thymus cell antigen 1, theta) promoter. It is not known what strain the transgenic vector was originally injected into, but this line has been backcrossed approximately 5 times to C57BL/6. This is line M53.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Parkinson's Disease Models
View Parkinson's Disease Models (66 strains)
008134 B6.Cg-Tg(THY1-SNCA*A30P)TS2Sud/J 008473 B6;SJL-Tg(THY1-SNCA*A30P)M30Sud/J 008389 C57BL/6-Tg(THY1-SNCA)1Sud/J 008474 STOCK Tg(THY1-SNCA*A53T)F53Sud/J 008132 STOCK Tg(THY1-Snca)M1mSud/J
Additional Web Information
Visit the Parkinson's Disease Resource site for helpful information on Parkinson's and research resources.
Phenotype
Phenotype Information
assigned by genotype
Tg(THY1-SNCA*A53T)M53Sud/0
involves: C57BL/6
- nervous system phenotype
- motor neuron degeneration (MGI Ref ID J:115193)
- mice show onset of motoneuron disease at ~5-6 months
This mouse can be used to support research in many areas including:
Neurobiology Research
Neurodegeneration
Parkinson's Disease
synuclein mutants
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(THY1-SNCA*A53T)M53Sud | ||
|---|---|---|---|
| Allele Name | transgene insertion M53, Thomas C Sudhof | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | M53; M70A53T; | ||
| Expressed Gene | SNCA, synuclein, alpha (non A4 component of amyloid precursor), human | ||
| Promoter | THY1, Thy-1 cell surface antigen, human | ||
| Molecular Note | This transgenic strain carries the mouse Snca (synuclein, alpha) gene under the control of a human THY1 (thymus cell antigen 1, theta) promoter. [MGI Ref ID J:115193] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(THY1-SNCA*A53T)M53Sud, F53Sud, Melt Curve Analysis
Sncatm1Sud, Standard PCR
Tg(THY1-SNCA*A53T)M53Sud, F53Sud, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Chandra S; Gallardo G; Fernandez-Chacon R; Schluter OM; Sudhof TC. 2005. Alpha-synuclein cooperates with CSPalpha in preventing neurodegeneration. Cell 123(3):383-96. [PubMed: 16269331] [MGI Ref ID J:115193]
Additional References
Tg(THY1-SNCA*A53T)M53Sud relatedGallardo G; Schluter OM; Sudhof TC. 2008. A molecular pathway of neurodegeneration linking alpha-synuclein to ApoE and Abeta peptides. Nat Neurosci 11(3):301-8. [PubMed: 18297066] [MGI Ref ID J:131733]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Mating System Noncarrier x Hemizygote (Female x Male) 26-JUL-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(THY1-SNCA*A53T)M53Sud
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Tg(THY1-SNCA*A53T)M53Sud x Noncarrier $297.85 Noncarrier x Hemizygous for Tg(THY1-SNCA*A53T)M53Sud
Additional Supply Details
| Pricing for International shipping destinations |
|
|
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(THY1-SNCA*A53T)M53Sud
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Tg(THY1-SNCA*A53T)M53Sud x Noncarrier $387.30 Noncarrier x Hemizygous for Tg(THY1-SNCA*A53T)M53Sud
Additional Supply Details
|
|
|
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and/or larger quantities of mice may be possible. Estimated ship dates for all orders provided within two business days following order placement. |
|---|---|
| Supply Notes |
|
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. In purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.