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| Hemizygous transgenic mice are viable and fertile and develop a Parkinson-like phenotype upon aging. Hind limb paralysis due to loss of motor neurons and a resting tremor are initially seen at about six months of age. No Lewy body-like pathology is noted. Cell death in the spinal cord (extensive) and brain are observed. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6.Cg-Tg(THY1-SNCA*A53T)1Sud/J (Changed: 22-JAN-08 ) Type Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Noncarrier x Hemizygote (Female x Male) 26-JUL-08 Species laboratory mouse Generation N5+F4 (18-NOV-09) Donating Investigator Thomas Sudhof, Stanford University School of Medicine Description
Hemizygous transgenic mice are viable and fertile and develop a Parkinson-like phenotype upon aging. Hind limb paralysis due to loss of motor neurons and a resting tremor are initially seen at about six months of age. No Lewy body-like pathology is noted. Cell death in the spinal cord (extensive) and brain are observed.Development
This transgenic strain carries the human SNCA (synuclein, alpha) gene under the control of a human THY1 (thymus cell antigen 1, theta) promoter. It is not known what strain the transgenic vector was originally injected into, but this line has been backcrossed approximately 5 times to C57BL/6. This is line M53.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of SNCA
008134 B6.Cg-Tg(THY1-SNCA*A30P)TS2Sud/J 004479 B6;C3-Tg(Prnp-SNCA*A53T)83Vle/J 008473 B6;SJL-Tg(THY1-SNCA*A30P)M30Sud/J 008389 C57BL/6-Tg(THY1-SNCA)1Sud/J 008474 STOCK Tg(THY1-SNCA*A53T)F53Sud/J View Strains carrying other alleles of SNCA (5 strains)
008134 B6.Cg-Tg(THY1-SNCA*A30P)TS2Sud/J 008473 B6;SJL-Tg(THY1-SNCA*A30P)M30Sud/J 008389 C57BL/6-Tg(THY1-SNCA)1Sud/J 008474 STOCK Tg(THY1-SNCA*A53T)F53Sud/J 008132 STOCK Tg(THY1-Snca)M1mSud/J
Phenotype
Phenotype Information
assigned by genotype
Tg(THY1-SNCA*A53T)M53Sud/0
involves: C57BL/6
- nervous system phenotype
- motor neuron degeneration (MGI Ref ID J:115193)
- mice show onset of motoneuron disease at ~5-6 months
This mouse can be used to support research in many areas including:
Neurobiology Research
Neurodegeneration
Parkinson's Disease
synuclein mutants
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(THY1-SNCA*A53T)M53Sud | ||
|---|---|---|---|
| Allele Name | transgene insertion M53, Thomas C Sudhof | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | M53; M70A53T; | ||
| Expressed Gene | SNCA, synuclein, alpha (non A4 component of amyloid precursor), human | ||
| Promoter | THY1, Thy-1 cell surface antigen, human | ||
| Molecular Note | This transgenic strain carries the mouse Snca (synuclein, alpha) gene under the control of a human THY1 (thymus cell antigen 1, theta) promoter. [MGI Ref ID J:115193] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(THY1-SNCA*A53T)M53Sud, F53Sud, Melt Curve Analysis
Sncatm1Sud, Standard PCR
Tg(THY1-SNCA*A53T)M53Sud, F53Sud, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Chandra S; Gallardo G; Fernandez-Chacon R; Schluter OM; Sudhof TC. 2005. Alpha-synuclein cooperates with CSPalpha in preventing neurodegeneration. Cell 123(3):383-96. [PubMed: 16269331] [MGI Ref ID J:115193]
Additional References
Tg(THY1-SNCA*A53T)M53Sud relatedGallardo G; Schluter OM; Sudhof TC. 2008. A molecular pathway of neurodegeneration linking alpha-synuclein to ApoE and Abeta peptides. Nat Neurosci 11(3):301-8. [PubMed: 18297066] [MGI Ref ID J:131733]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Mating System Noncarrier x Hemizygote (Female x Male) 26-JUL-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(THY1-SNCA*A53T)M53Sud
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Tg(THY1-SNCA*A53T)M53Sud x Noncarrier $297.85 Noncarrier x Hemizygous for Tg(THY1-SNCA*A53T)M53Sud
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(THY1-SNCA*A53T)M53Sud
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Tg(THY1-SNCA*A53T)M53Sud x Noncarrier $387.30 Noncarrier x Hemizygous for Tg(THY1-SNCA*A53T)M53Sud
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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