Strain Name:

STOCK Gt(ROSA)26Sortm1(Notch1)Dam/J

Stock Number:

008159

Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
When used in conjunction with a Cre recombinase-expressing strain, these RosaN1-IC (or RosaNotch) mutant mice may be useful in generating conditional mutations for studying the effects of Notch pathway activation.

Description

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Mating SystemHomozygote x Homozygote         (Female x Male)   12-NOV-08
Specieslaboratory mouse
GenerationF?+F3 (18-NOV-09)
 
Donating Investigator Douglas Melton,   Harvard University

Description
These mice contain a sequence encoding an intracellular portion of the mouse Notch1 gene (amino acids 1749-2293), but lacking the c-terminal PEST domain, and Green Fluorescent Protein, GFP, inserted into the GT(ROSA)26Sor locus. Expression of the Notch1 fragment and GFP is blocked by a loxP-flanked STOP fragment placed between the coding sequence and the GT(ROSA)26Sor promoter. The GFP expression is localized to the nucleus by an IRES sequence. The truncated cytoplasmic fragment encoded by the Notch1 sequence causes constitutive signaling activity. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants for studying the effects of Notch pathway activation. Homozygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.

For example, when crossed to a strain expressing a tamoxifen inducible Cre recombinase in all cells that express Shh (see Stock No. 005623), this mutant mouse strain may be useful in studies of Notch signalling.

Development
A targeting vector containing a loxP- flanked neo-STOP cassette, mouse Notch1 sequence encoding amino acids 1749-2293 and GFP was inserted into the GT(ROSA)26Sor locus. The construct was introduced into 129S4/SvJaeSor-derived AK7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into receipient blastocysts. Resulting chimeric male animals were crossed to C57BL/6 females. The mice were then crossed to C57BL/6J for an unknown number of generations and maintained on the outbred ICR background.

Control Information

  Control
   None Available
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Gt(ROSA)26Sor
002292   129-Gt(ROSA)26Sor/J
006053   129-Gt(ROSA)26Sortm1Luo/J
006067   129-Gt(ROSA)26Sortm2Luo/J
006041   129-Gt(ROSA)26Sortm3Luo/J
003310   129S-Gt(ROSA)26Sortm1Sor/J
009043   129S-Gt(ROSA)26Sortm2Tyj/J
003946   129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J
007689   129S4/SvJaeSor-Gt(ROSA)26Sortm4(attB/attP)Sor/J
010633   B6(Cg)-Gt(ROSA)26Sortm1(CAG-taulacZ)Bene/J
007676   B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J
007708   B6.129-Gt(ROSA)26Sortm1(HD*103Q)Xwy/J
008463   B6.129-Gt(ROSA)26Sortm1(cre/ESR1)Tyj/J
006071   B6.129-Gt(ROSA)26Sortm1Luo/J
006080   B6.129-Gt(ROSA)26Sortm2Luo/J
006075   B6.129-Gt(ROSA)26Sortm3Luo/J
009669   B6.129P2-Gt(ROSA)26Sortm1(DTA)Lky/J
008513   B6.129P2-Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde/J
008600   B6.129P2-Gt(ROSA)26Sortm1(tTA)Roos/J
009086   B6.129S4-Gt(ROSA)26Sortm1(FLP1)Dym/RainJ
003474   B6.129S4-Gt(ROSA)26Sortm1Sor/J
009044   B6.129S4-Gt(ROSA)26Sortm2Tyj/J
007743   B6.129S4-Gt(ROSA)26Sortm3(phiC31*)Sor/J
002192   B6.129S7-Gt(ROSA)26Sor/J
006148   B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J
006965   B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae/J
005670   B6.Cg-Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
007914   B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J
007920   B6.Cg-Gt(ROSA)26Sortm2(CAG-EYFP)Hze/J
007903   B6.Cg-Gt(ROSA)26Sortm3(CAG-EYFP)Hze/J
007906   B6.Cg-Gt(ROSA)26Sortm6(CAG-ZsGreen1)Hze/J
007909   B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J
007897   B6.Cg-Tg(Gt(ROSA)26Sor-EGFP)I1Able/J
008883   B6;129-Gt(ROSA)26Sortm1(SNCA*A53T)Djmo/TmdJ
004847   B6;129-Gt(ROSA)26Sortm1(cre/Esr1)Nat/J
006911   B6;129-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm2(tetO-Pou5f1)Jae/J
008516   B6;129-Gt(ROSA)26Sortm1Joe/J
003504   B6;129-Gt(ROSA)26Sortm1Sho/J
008889   B6;129-Gt(ROSA)26Sortm2(SNCA*119)Djmo/TmdJ
004077   B6;129-Gt(ROSA)26Sortm2Sho/J
008886   B6;129-Gt(ROSA)26Sortm3(SNCA*E46K)Djmo/TmdJ
010523   B6;129P2-Gt(ROSA)26Sortm1(CAG-ALPP)Fawa/J
002073   B6;129S-Gt(ROSA)26Sor/J
003309   B6;129S4-Gt(ROSA)26Sortm1Sor/J
004598   B6;129S4-Gt(ROSA)26Sortm2Dym/J
007670   B6;129S4-Gt(ROSA)26Sortm3(phiC31*)Sor/J
007908   B6;129S6-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J
007905   B6;129S6-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J
002955   C.129S7-Gt(ROSA)26Sor/J
007900   C57BL/6-Gt(ROSA)26Sortm1(HBEGF)Awai/J
008242   C57BL/6-Gt(ROSA)26Sortm1(Ikbkb)Rsky/J
008517   C57BL/6-Gt(ROSA)26Sortm3(CAG-MIRN17-92,-EGFP)Rsky/J
005420   C;129S7 Gt(ROSA)26Sor-Bmp5cfe-se7J/J
008040   CBy.B6-Gt(ROSA)26Sortm1(HBEGF)Awai/J
007898   CBy.Cg-Tg(Gt(ROSA)26Sor-EGFP)I1Able/J
009427   FVB.129S4(B6)-Gt(ROSA)26Sortm1Sor/J
005125   FVB.129S6(B6)-Gt(ROSA)26Sortm1(Luc)Kael/J
006206   FVB.129S6-Gt(ROSA)26Sortm2(HIF1A/luc)Kael/J
010920   FVB;129P2-Gt(ROSA)26Sortm1(birA)Mejr/J
006331   STOCK Gt(ROSA)26Sortm1(DTA)Jpmb/J
005130   STOCK Gt(ROSA)26Sortm1(Smo/EYFP)Amc/J
005572   STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
007576   STOCK Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J
007577   STOCK Tg(Gt(ROSA)26Sor-BCHE*G117H)837Loc/J
007896   STOCK Tg(Gt(ROSA)26Sor-EGFP)I1Able/J
View Strains carrying other alleles of Gt(ROSA)26Sor     (64 strains)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms
      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Gt(ROSA)26Sortm1(Notch1)Dam/Gt(ROSA)26Sortm1(Notch1)Dam

        involves: 129S4/SvJae * C57BL/6
  • normal phenotype
  • no abnormal phenotype detected (MGI Ref ID J:86975)

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

Gt(ROSA)26Sortm1(Notch1)Dam/Gt(ROSA)26Sor+ Shhtm2(cre/ESR1)Cjt/Shh+

        involves: 129S4/SvJaeSor   (conditional)
  • vision/eye phenotype
  • *normal* vision/eye phenotype (MGI Ref ID J:118372)
    • retina size is normal
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cell Biology Research
Signal Transduction

Research Tools
Cre-lox System
      loxP-flanked Sequences
Developmental Biology Research
      Cre-lox System
Genetics Research
      Tissue/Cell Markers: Cre-lox System

Genes & Alleles

Gene & Allele Information

 
Allele Symbol Gt(ROSA)26Sortm1(Notch1)Dam
Allele Name targeted mutation 1, Douglas A Melton
Allele Type Targeted (knock-in)
Common Name(s) Lox-stop-Lox-RosaNICD-ires-GFP; R-NICD; Rosa-NotchIC-IRES-GFP; Rosa26NIC; RosaN1-IC; RosaNICD; RosaNotch;
Mutation Made By Douglas Melton,   Harvard University
Strain of Origin129S4/SvJaeSor
ES Cell Line NameAK7
ES Cell Line Strain129S4/SvJaeSor
Gene Symbol and Name Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano
Chromosome 6
Gene Common Name(s) AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26;
Molecular Note A targeting vector containing a floxed neo cassette followed by a mouse Notch1 sequence fragment and GFP was inserted at the endogenous locus. Excision of the floxed neo cassette leads to the expression of the Notch1 sequence encoding an intracellular portion of NOTCH1 (amino acids 1749-2293), but lacking the c-terminal PEST domain. IRES preceded the sequence encoding the nuclear-localized enhanced GFP. [MGI Ref ID J:86975]

Genotyping

Genotyping Information

Genotyping Protocols

Gt(ROSA)26Sortm1(Notch1)Dam, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Murtaugh LC; Stanger BZ; Kwan KM; Melton DA. 2003. Notch signaling controls multiple steps of pancreatic differentiation. Proc Natl Acad Sci U S A 100(25):14920-5. [PubMed: 14657333]  [MGI Ref ID J:86975]

Additional References

Gt(ROSA)26Sortm1(Notch1)Dam related

Blanpain C; Lowry WE; Pasolli HA; Fuchs E. 2006. Canonical notch signaling functions as a commitment switch in the epidermal lineage. Genes Dev 20(21):3022-35. [PubMed: 17079689]  [MGI Ref ID J:114702]

Cheng HT; Kim M; Valerius MT; Surendran K; Schuster-Gossler K; Gossler A; McMahon AP; Kopan R. 2007. Notch2, but not Notch1, is required for proximal fate acquisition in the mammalian nephron. Development 134(4):801-11. [PubMed: 17229764]  [MGI Ref ID J:119907]

De La O JP; Emerson LL; Goodman JL; Froebe SC; Illum BE; Curtis AB; Murtaugh LC. 2008. Notch and Kras reprogram pancreatic acinar cells to ductal intraepithelial neoplasia. Proc Natl Acad Sci U S A 105(48):18907-12. [PubMed: 19028876]  [MGI Ref ID J:142188]

Feller J; Schneider A; Schuster-Gossler K; Gossler A. 2008. Noncyclic Notch activity in the presomitic mesoderm demonstrates uncoupling of somite compartmentalization and boundary formation. Genes Dev 22(16):2166-71. [PubMed: 18708576]  [MGI Ref ID J:138982]

Fre S; Huyghe M; Mourikis P; Robine S; Louvard D; Artavanis-Tsakonas S. 2005. Notch signals control the fate of immature progenitor cells in the intestine. Nature 435(7044):964-8. [PubMed: 15959516]  [MGI Ref ID J:99364]

Fre S; Pallavi SK; Huyghe M; Lae M; Janssen KP; Robine S; Artavanis-Tsakonas S; Louvard D. 2009. Notch and Wnt signals cooperatively control cell proliferation and tumorigenesis in the intestine. Proc Natl Acad Sci U S A 106(15):6309-14. [PubMed: 19251639]  [MGI Ref ID J:147579]

Greenwood AL; Li S; Jones K; Melton DA. 2007. Notch signaling reveals developmental plasticity of Pax4(+) pancreatic endocrine progenitors and shunts them to a duct fate. Mech Dev 124(2):97-107. [PubMed: 17196797]  [MGI Ref ID J:119944]

Guseh JS; Bores SA; Stanger BZ; Zhou Q; Anderson WJ; Melton DA; Rajagopal J. 2009. Notch signaling promotes airway mucous metaplasia and inhibits alveolar development. Development 136(10):1751-9. [PubMed: 19369400]  [MGI Ref ID J:148016]

Iulianella A; Sharma M; Vanden Heuvel GB; Trainor PA. 2009. Cux2 functions downstream of Notch signaling to regulate dorsal interneuron formation in the spinal cord. Development 136(14):2329-34. [PubMed: 19542352]  [MGI Ref ID J:150348]

Jadhav AP; Cho SH; Cepko CL. 2006. Notch activity permits retinal cells to progress through multiple progenitor states and acquire a stem cell property. Proc Natl Acad Sci U S A 103(50):18998-9003. [PubMed: 17148603]  [MGI Ref ID J:118372]

Jayasena CS; Ohyama T; Segil N; Groves AK. 2008. Notch signaling augments the canonical Wnt pathway to specify the size of the otic placode. Development 135(13):2251-61. [PubMed: 18495817]  [MGI Ref ID J:137096]

Kim YW; Koo BK; Jeong HW; Yoon MJ; Song R; Shin J; Jeong DC; Kim SH; Kong YY. 2008. Defective Notch activation in microenvironment leads to myeloproliferative disease. Blood 112(12):4628-38. [PubMed: 18818392]  [MGI Ref ID J:143346]

Mead TJ; Yutzey KE. 2009. Notch pathway regulation of chondrocyte differentiation and proliferation during appendicular and axial skeleton development. Proc Natl Acad Sci U S A 106(34):14420-5. [PubMed: 19590010]  [MGI Ref ID J:151888]

Rowan S; Conley KW; Le TT; Donner AL; Maas RL; Brown NL. 2008. Notch signaling regulates growth and differentiation in the mammalian lens. Dev Biol 321(1):111-22. [PubMed: 18588871]  [MGI Ref ID J:138714]

Sorensen I; Adams RH; Gossler A. 2009. DLL1-mediated Notch activation regulates endothelial identity in mouse fetal arteries. Blood 113(22):5680-8. [PubMed: 19144989]  [MGI Ref ID J:148902]

Stanger BZ; Datar R; Murtaugh LC; Melton DA. 2005. Direct regulation of intestinal fate by Notch. Proc Natl Acad Sci U S A 102(35):12443-8. [PubMed: 16107537]  [MGI Ref ID J:101154]

Tang H; Brennan J; Karl J; Hamada Y; Raetzman L; Capel B. 2008. Notch signaling maintains Leydig progenitor cells in the mouse testis. Development 135(22):3745-53. [PubMed: 18927153]  [MGI Ref ID J:143587]

Yoon KJ; Koo BK; Im SK; Jeong HW; Ghim J; Kwon MC; Moon JS; Miyata T; Kong YY. 2008. Mind bomb 1-expressing intermediate progenitors generate notch signaling to maintain radial glial cells. Neuron 58(4):519-31. [PubMed: 18498734]  [MGI Ref ID J:145294]

Zanotti S; Smerdel-Ramoya A; Stadmeyer L; Durant D; Radtke F; Canalis E. 2008. Notch inhibits osteoblast differentiation and causes osteopenia. Endocrinology 149(8):3890-9. [PubMed: 18420737]  [MGI Ref ID J:138080]

Zong Y; Panikkar A; Xu J; Antoniou A; Raynaud P; Lemaigre F; Stanger BZ. 2009. Notch signaling controls liver development by regulating biliary differentiation. Development 136(10):1727-39. [PubMed: 19369401]  [MGI Ref ID J:148015]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX11

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as homozygotes.
Mating SystemHomozygote x Homozygote         (Female x Male)   12-NOV-08
Diet Information LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Weeks of AgePrice (US dollars $)GenderGenotypes Provided
Individual Mouse $160.40Female or MaleHomozygous for Gt(ROSA)26Sortm1(Notch1)Dam
Pairs /Price (US dollars $)Pair Genotype
$320.80Homozygous for Gt(ROSA)26Sortm1(Notch1)Dam x Homozygous for Gt(ROSA)26Sortm1(Notch1)Dam

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Weeks of AgePrice (US dollars $)GenderGenotypes Provided
Individual Mouse $208.60Female or MaleHomozygous for Gt(ROSA)26Sortm1(Notch1)Dam
Pairs /Price (US dollars $)Pair Genotype
$417.10Homozygous for Gt(ROSA)26Sortm1(Notch1)Dam x Homozygous for Gt(ROSA)26Sortm1(Notch1)Dam

Additional Supply Details

Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement.
Supply Notes

Control Information

  Control
   None Available
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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