| |||||||||||||||
| When used in conjunction with a Cre recombinase-expressing strain, these RosaN1-IC (or RosaNotch) mutant mice may be useful in generating conditional mutations for studying the effects of Notch pathway activation. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote (Female x Male) 12-NOV-08 Species laboratory mouse Generation F?+F3 (18-NOV-09) Donating Investigator Douglas Melton, Harvard University Description
These mice contain a sequence encoding an intracellular portion of the mouse Notch1 gene (amino acids 1749-2293), but lacking the c-terminal PEST domain, and Green Fluorescent Protein, GFP, inserted into the GT(ROSA)26Sor locus. Expression of the Notch1 fragment and GFP is blocked by a loxP-flanked STOP fragment placed between the coding sequence and the GT(ROSA)26Sor promoter. The GFP expression is localized to the nucleus by an IRES sequence. The truncated cytoplasmic fragment encoded by the Notch1 sequence causes constitutive signaling activity. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants for studying the effects of Notch pathway activation. Homozygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.For example, when crossed to a strain expressing a tamoxifen inducible Cre recombinase in all cells that express Shh (see Stock No. 005623), this mutant mouse strain may be useful in studies of Notch signalling.
Development
A targeting vector containing a loxP- flanked neo-STOP cassette, mouse Notch1 sequence encoding amino acids 1749-2293 and GFP was inserted into the GT(ROSA)26Sor locus. The construct was introduced into 129S4/SvJaeSor-derived AK7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into receipient blastocysts. Resulting chimeric male animals were crossed to C57BL/6 females. The mice were then crossed to C57BL/6J for an unknown number of generations and maintained on the outbred ICR background.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Gt(ROSA)26Sor
View Strains carrying other alleles of Gt(ROSA)26Sor (64 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Gt(ROSA)26Sortm1(Notch1)Dam/Gt(ROSA)26Sortm1(Notch1)Dam
involves: 129S4/SvJae * C57BL/6
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:86975)
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Gt(ROSA)26Sortm1(Notch1)Dam/Gt(ROSA)26Sor+ Shhtm2(cre/ESR1)Cjt/Shh+
involves: 129S4/SvJaeSor (conditional)
- vision/eye phenotype
- *normal* vision/eye phenotype (MGI Ref ID J:118372)
- retina size is normal
This mouse can be used to support research in many areas including:
Cell Biology Research
Signal Transduction
Research Tools
Cre-lox System
loxP-flanked Sequences
Developmental Biology Research
Cre-lox System
Genetics Research
Tissue/Cell Markers: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Gt(ROSA)26Sortm1(Notch1)Dam | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Douglas A Melton | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | Lox-stop-Lox-RosaNICD-ires-GFP; R-NICD; Rosa-NotchIC-IRES-GFP; Rosa26NIC; RosaN1-IC; RosaNICD; RosaNotch; | ||
| Mutation Made By | Douglas Melton, Harvard University | ||
| Strain of Origin | 129S4/SvJaeSor | ||
| ES Cell Line Name | AK7 | ||
| ES Cell Line Strain | 129S4/SvJaeSor | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
| Molecular Note | A targeting vector containing a floxed neo cassette followed by a mouse Notch1 sequence fragment and GFP was inserted at the endogenous locus. Excision of the floxed neo cassette leads to the expression of the Notch1 sequence encoding an intracellular portion of NOTCH1 (amino acids 1749-2293), but lacking the c-terminal PEST domain. IRES preceded the sequence encoding the nuclear-localized enhanced GFP. [MGI Ref ID J:86975] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sortm1(Notch1)Dam, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Murtaugh LC; Stanger BZ; Kwan KM; Melton DA. 2003. Notch signaling controls multiple steps of pancreatic differentiation. Proc Natl Acad Sci U S A 100(25):14920-5. [PubMed: 14657333] [MGI Ref ID J:86975]
Additional References
Gt(ROSA)26Sortm1(Notch1)Dam relatedBlanpain C; Lowry WE; Pasolli HA; Fuchs E. 2006. Canonical notch signaling functions as a commitment switch in the epidermal lineage. Genes Dev 20(21):3022-35. [PubMed: 17079689] [MGI Ref ID J:114702]
Cheng HT; Kim M; Valerius MT; Surendran K; Schuster-Gossler K; Gossler A; McMahon AP; Kopan R. 2007. Notch2, but not Notch1, is required for proximal fate acquisition in the mammalian nephron. Development 134(4):801-11. [PubMed: 17229764] [MGI Ref ID J:119907]
De La O JP; Emerson LL; Goodman JL; Froebe SC; Illum BE; Curtis AB; Murtaugh LC. 2008. Notch and Kras reprogram pancreatic acinar cells to ductal intraepithelial neoplasia. Proc Natl Acad Sci U S A 105(48):18907-12. [PubMed: 19028876] [MGI Ref ID J:142188]
Feller J; Schneider A; Schuster-Gossler K; Gossler A. 2008. Noncyclic Notch activity in the presomitic mesoderm demonstrates uncoupling of somite compartmentalization and boundary formation. Genes Dev 22(16):2166-71. [PubMed: 18708576] [MGI Ref ID J:138982]
Fre S; Huyghe M; Mourikis P; Robine S; Louvard D; Artavanis-Tsakonas S. 2005. Notch signals control the fate of immature progenitor cells in the intestine. Nature 435(7044):964-8. [PubMed: 15959516] [MGI Ref ID J:99364]
Fre S; Pallavi SK; Huyghe M; Lae M; Janssen KP; Robine S; Artavanis-Tsakonas S; Louvard D. 2009. Notch and Wnt signals cooperatively control cell proliferation and tumorigenesis in the intestine. Proc Natl Acad Sci U S A 106(15):6309-14. [PubMed: 19251639] [MGI Ref ID J:147579]
Greenwood AL; Li S; Jones K; Melton DA. 2007. Notch signaling reveals developmental plasticity of Pax4(+) pancreatic endocrine progenitors and shunts them to a duct fate. Mech Dev 124(2):97-107. [PubMed: 17196797] [MGI Ref ID J:119944]
Guseh JS; Bores SA; Stanger BZ; Zhou Q; Anderson WJ; Melton DA; Rajagopal J. 2009. Notch signaling promotes airway mucous metaplasia and inhibits alveolar development. Development 136(10):1751-9. [PubMed: 19369400] [MGI Ref ID J:148016]
Iulianella A; Sharma M; Vanden Heuvel GB; Trainor PA. 2009. Cux2 functions downstream of Notch signaling to regulate dorsal interneuron formation in the spinal cord. Development 136(14):2329-34. [PubMed: 19542352] [MGI Ref ID J:150348]
Jadhav AP; Cho SH; Cepko CL. 2006. Notch activity permits retinal cells to progress through multiple progenitor states and acquire a stem cell property. Proc Natl Acad Sci U S A 103(50):18998-9003. [PubMed: 17148603] [MGI Ref ID J:118372]
Jayasena CS; Ohyama T; Segil N; Groves AK. 2008. Notch signaling augments the canonical Wnt pathway to specify the size of the otic placode. Development 135(13):2251-61. [PubMed: 18495817] [MGI Ref ID J:137096]
Kim YW; Koo BK; Jeong HW; Yoon MJ; Song R; Shin J; Jeong DC; Kim SH; Kong YY. 2008. Defective Notch activation in microenvironment leads to myeloproliferative disease. Blood 112(12):4628-38. [PubMed: 18818392] [MGI Ref ID J:143346]
Mead TJ; Yutzey KE. 2009. Notch pathway regulation of chondrocyte differentiation and proliferation during appendicular and axial skeleton development. Proc Natl Acad Sci U S A 106(34):14420-5. [PubMed: 19590010] [MGI Ref ID J:151888]
Rowan S; Conley KW; Le TT; Donner AL; Maas RL; Brown NL. 2008. Notch signaling regulates growth and differentiation in the mammalian lens. Dev Biol 321(1):111-22. [PubMed: 18588871] [MGI Ref ID J:138714]
Sorensen I; Adams RH; Gossler A. 2009. DLL1-mediated Notch activation regulates endothelial identity in mouse fetal arteries. Blood 113(22):5680-8. [PubMed: 19144989] [MGI Ref ID J:148902]
Stanger BZ; Datar R; Murtaugh LC; Melton DA. 2005. Direct regulation of intestinal fate by Notch. Proc Natl Acad Sci U S A 102(35):12443-8. [PubMed: 16107537] [MGI Ref ID J:101154]
Tang H; Brennan J; Karl J; Hamada Y; Raetzman L; Capel B. 2008. Notch signaling maintains Leydig progenitor cells in the mouse testis. Development 135(22):3745-53. [PubMed: 18927153] [MGI Ref ID J:143587]
Yoon KJ; Koo BK; Im SK; Jeong HW; Ghim J; Kwon MC; Moon JS; Miyata T; Kong YY. 2008. Mind bomb 1-expressing intermediate progenitors generate notch signaling to maintain radial glial cells. Neuron 58(4):519-31. [PubMed: 18498734] [MGI Ref ID J:145294]
Zanotti S; Smerdel-Ramoya A; Stadmeyer L; Durant D; Radtke F; Canalis E. 2008. Notch inhibits osteoblast differentiation and causes osteopenia. Endocrinology 149(8):3890-9. [PubMed: 18420737] [MGI Ref ID J:138080]
Zong Y; Panikkar A; Xu J; Antoniou A; Raynaud P; Lemaigre F; Stanger BZ. 2009. Notch signaling controls liver development by regulating biliary differentiation. Development 136(10):1727-39. [PubMed: 19369401] [MGI Ref ID J:148015]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as homozygotes. Mating System Homozygote x Homozygote (Female x Male) 12-NOV-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $160.40 Female or Male Homozygous for Gt(ROSA)26Sortm1(Notch1)Dam
Pairs /Price (US dollars $) Pair Genotype $320.80 Homozygous for Gt(ROSA)26Sortm1(Notch1)Dam x Homozygous for Gt(ROSA)26Sortm1(Notch1)Dam
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $208.60 Female or Male Homozygous for Gt(ROSA)26Sortm1(Notch1)Dam
Pairs /Price (US dollars $) Pair Genotype $417.10 Homozygous for Gt(ROSA)26Sortm1(Notch1)Dam x Homozygous for Gt(ROSA)26Sortm1(Notch1)Dam
Additional Supply Details
|
|
|
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
|---|---|
| Supply Notes |
|
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. In purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.