Strain Name: |
STOCK Gt(ROSA)26Sortm1(Notch1)Dam/J |
|---|---|
Stock Number: |
008159 |
Availability: | Under Development for Distribution Colony |
| To register your interest in this strain go to the Strain Interest Form. |
General Terms and Conditions |
| Genes & Alleles | Gt(ROSA)26Sor; Gt(ROSA)26Sortm1(Notch1)Dam; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Mating System Heterozygote x Heterozygote (Female x Male) Species laboratory mouse Donating Investigator Douglas Melton, Harvard University Generation F?+ (21-JUL-08) Strain Description
These mice contain a sequence encoding an intracellular portion of the mouse Notch1 gene (amino acids 1749-2293), but lacking the c-terminal PEST domain, and Green Fluorescent Protein, GFP, inserted into the GT(ROSA)26Sor locus. Expression of the Notch1 fragment and GFP is blocked by a loxP-flanked STOP fragment placed between the coding sequence and the GT(ROSA)26Sor promoter. The GFP expression is localized to the nucleus by an IRES sequence. The truncated cytoplasmic fragment encoded by the Notch1 sequence causes constitutive signaling activity. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants for studying the effects of Notch pathway activation. Homozygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.For example, when crossed to a strain expressing a tamoxifen inducible Cre recombinase in all cells that express Shh (see Stock No. 005623), this mutant mouse strain may be useful in studies of Notch signalling.
Strain Development
A targeting vector containing a loxP- flanked neo-STOP cassette, mouse Notch1 sequence encoding amino acids 1749-2293 and GFP was inserted into the GT(ROSA)26Sor locus. The construct was introduced into 129S4/SvJaeSor-derived AK7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into receipient blastocysts. Resulting chimeric male animals were crossed to C57BL/6 females. The mice were then crossed to C57BL/6J for an unknown number of generations and maintained on the outbred ICR background.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Gt(ROSA)26Sortm1(Notch1)Dam | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Douglas A Melton | ||
| Common Name(s) | Lox-stop-Lox-RosaNICD-ires-GFP; RosaN1-IC; RosaNotch; | ||
| Mutation Made By | Douglas Melton, Harvard University | ||
| Strain of Origin | 129S4/SvJaeSor | ||
| ES Cell Line Name | AK7 | ||
| ES Cell Line Strain | 129S4/SvJaeSor | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
| Molecular Note | A targeting vector containing a floxed neo cassette followed by a mouse Notch1 sequence fragment and GFP was inserted at the endogenous locus. Excision of the floxed neo cassette leads to the expression of the Notch1 sequence encoding an intracellular portion of NOTCH1 (amino acids 1749-2293), but lacking the c-terminal PEST domain. IRES preceded the sequence encoding the nuclear-localized enhanced GFP. [MGI Ref ID J:86975] | ||
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Gt(ROSA)26Sortm1(Notch1)Dam
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice can be bred as homozygotes. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Gt(ROSA)26Sor
View Strains carrying other alleles of Gt(ROSA)26Sor (36 strains)
Additional Web Information
Cre-lox or FLP-FRT Systems
Research Applications
This mouse can be used to support research in many areas including:
Cell Biology Research
Signal Transduction
Research Tools
Cre-lox System (loxP-flanked Sequences)
Developmental Biology Research (Cre-lox System)
Genetics Research (Tissue/Cell Markers: Cre-lox System)
References
Selected Reference(s)
Additional ReferencesMurtaugh LC; Stanger BZ; Kwan KM; Melton DA. 2003. Notch signaling controls multiple steps of pancreatic differentiation. Proc Natl Acad Sci U S A 100(25):14920-5. [PubMed: 14657333] [MGI Ref ID J:86975]
Price and Supply Information
| Strain Name: | STOCK Gt(ROSA)26Sortm1(Notch1)Dam/J |
| Stock Number: | 008159 |
This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date: 29-SEP-08
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
View All Strains Under Development
Supply Details
| Standard Supply | Under Development for Distribution Colony |
|---|---|
| Supply Notes |
This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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