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| Congenic NOD mice hemizygous for Enhanced Green Florescent Protein, EGFP, under the control of the Mouse Insulin I promoter, InsI, (MIP-GFP) express green florescence in tissues where insulin I is normally detected, specifically in pancreatic beta-cells. Transgenic mice are reported to develop diabetes at a similar or higher rate and kinetics as wildtype controls. Transgenic mice may be useful in studies of diabetes and pancreatic beta islet cell biology. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Noncarrier x Hemizygote (Female x Male) 02-AUG-12 Mating System Hemizygote x Noncarrier (Female x Male) 02-AUG-12 Species laboratory mouse Background Strain NOD Donor Strain C57BL/6 H2 Haplotype g7 Generation N14+N5 (06-DEC-12)
Generation DefinitionsDonating Investigator Qizhi Tang, U of California Appearance
albino
Related Genotype: A/A Tyrc/TyrcDescription
Mice hemizygous for this "MIP-GFP" transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Green Fluorescent Protein under the control of mouse insulin 1 promoter. Fluorescence is detected in tissues where insulin I is normally expressed; fluorescent protein expression in pancreatic beta-cells is evident from embryonic day (E)13.5 through adult. The human growth hormone sequence in the transgenic insert enhances expression of the EGFP but is not expressed.The donating investigator reports NOD transgenic female mice develop insulitis and autoimmune diabetes at a similar rate and kinetics as female wildtype controls, 62% and 58% respectively, while surprisingly, transgenic males develop insulitis and diabetes at a higher rate than male wildtype controls, 65% and 10% respectively. This transgenic MIP-GFP mouse strain is useful in studies of diabetes and pancreatic beta islet cell biology.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Development
A transgenic construct containing the coding region of the EGFP gene and a 2.1kb sequence of the human growth hormone to confer high expression of the EGFP, under the control of the mouse insulin 1 gene promoter was injected into fertilized CD1 mouse eggs. Founder line 1 was established on a C57BL/6 background prior to mating to NOD. In 2008, the Type 1 Diabetes Resource received this transgenic NOD strain at generation N14 and mated to NOD/ShiLtJ (Stock No. 001976) for one generation prior to sibling mating.
Control Information
| Control | ||
|---|---|---|
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (357 strains)
006864 B6.Cg-Tg(Ins1-EGFP)1Hara/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
GFP relatedDiabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains
NOD Transgenics
Immunology, Inflammation and Autoimmunity Research
Autoimmunity
Type 1 Diabetes
Research Tools
Diabetes and Obesity Research
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers
Tissue/Cell Markers: pancreatic beta cells
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Ins1-EGFP)1Hara | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Manami Hara | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | MIP-EGFP; MIP-GFP; MIP-GFP (line 1); MIP-GFP (line1); MIP:GFP; | ||
| Mutation Made By | Manami Hara, University of Chicago | ||
| Strain of Origin | CD-1 | ||
| Site of Expression | EGFP expression is detected in tissues where insulin I is normally expressed; fluorescent protein expression in pancreatic beta-cells is evident from embryonic day (E)13.5 through adulthood. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Ins1, insulin I, mouse, laboratory | ||
| Molecular Note | A transgenic construct containing the coding region of the EGFP gene and a 2.1kb sequence of the human growth hormone to confer high expression of the EGFP, under the control of the mouse insulin 1 gene promoter (a 8.5 kb region including -8.5 to +12 bp relative to the transcriptional start site) was injected into fertilized CD1 mouse eggs. Founder line 1 was subsequently established. Fluorescence is detected in tissues where insulin I is normally expressed; fluorescent protein expression in pancreatic beta-cells is evident from embryonic day (E)13.5 through adult. [MGI Ref ID J:99450] | ||
| Gene Symbol and Name | Tg(Ins1-EGFP)1Hara, transgene insertion 1, Manami Hara | ||
| Chromosome | UN | ||
| Gene Common Name(s) | MIP-GFP; MIP-GFP (line 1); MIP-GFP (line1); MIP:GFP; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Melt Curve Analysis
Fluorescent Proteins (Generic GFP), Standard PCR
Fluorescent Proteins -- Generic GFP, QPCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Chase LG; Ulloa-Montoya F; Kidder BL; Verfaillie CM. 2007. Islet-derived fibroblast-like cells are not derived via epithelial-mesenchymal transition from Pdx-1 or insulin-positive cells. Diabetes 56(1):3-7. [PubMed: 17110468] [MGI Ref ID J:121938]
Hara M; Dizon RF; Glick BS; Lee CS; Kaestner KH; Piston DW; Bindokas VP. 2006. Imaging pancreatic beta-cells in the intact pancreas. Am J Physiol Endocrinol Metab 290(5):E1041-7. [PubMed: 16368785] [MGI Ref ID J:120245]
Hara M; Wang X; Kawamura T; Bindokas VP; Dizon RF; Alcoser SY; Magnuson MA; Bell GI. 2003. Transgenic mice with green fluorescent protein-labeled pancreatic beta -cells. Am J Physiol Endocrinol Metab 284(1):E177-83. [PubMed: 12388130] [MGI Ref ID J:99450]
Hauser AE; Junt T; Mempel TR; Sneddon MW; Kleinstein SH; Henrickson SE; von Andrian UH; Shlomchik MJ; Haberman AM. 2007. Definition of germinal-center B cell migration in vivo reveals predominant intrazonal circulation patterns. Immunity 26(5):655-67. [PubMed: 17509908] [MGI Ref ID J:123545]
Melli K; Friedman RS; Martin AE; Finger EB; Miao G; Szot GL; Krummel MF; Tang Q. 2009. Amplification of autoimmune response through induction of dendritic cell maturation in inflamed tissues. J Immunol 182(5):2590-600. [PubMed: 19234153] [MGI Ref ID J:145529]
Additional References
Tg(Ins1-EGFP)1Hara relatedBasford CL; Prentice KJ; Hardy AB; Sarangi F; Micallef SJ; Li X; Guo Q; Elefanty AG; Stanley EG; Keller G; Allister EM; Nostro MC; Wheeler MB. 2012. The functional and molecular characterisation of human embryonic stem cell-derived insulin-positive cells compared with adult pancreatic beta cells. Diabetologia 55(2):358-71. [PubMed: 22075915] [MGI Ref ID J:181645]
Burlison JS; Long Q; Fujitani Y; Wright CV; Magnuson MA. 2008. Pdx-1 and Ptf1a concurrently determine fate specification of pancreatic multipotent progenitor cells. Dev Biol 316(1):74-86. [PubMed: 18294628] [MGI Ref ID J:135698]
Chen H; Gu X; Liu Y; Wang J; Wirt SE; Bottino R; Schorle H; Sage J; Kim SK. 2011. PDGF signalling controls age-dependent proliferation in pancreatic beta-cells. Nature 478(7369):349-55. [PubMed: 21993628] [MGI Ref ID J:177411]
Diao J; Allister EM; Koshkin V; Lee SC; Bhattacharjee A; Tang C; Giacca A; Chan CB; Wheeler MB. 2008. UCP2 is highly expressed in pancreatic alpha-cells and influences secretion and survival. Proc Natl Acad Sci U S A 105(33):12057-62. [PubMed: 18701716] [MGI Ref ID J:138987]
Inada A; Nienaber C; Katsuta H; Fujitani Y; Levine J; Morita R; Sharma A; Bonner-Weir S. 2008. Carbonic anhydrase II-positive pancreatic cells are progenitors for both endocrine and exocrine pancreas after birth. Proc Natl Acad Sci U S A 105(50):19915-9. [PubMed: 19052237] [MGI Ref ID J:142917]
Jacobson DA; Mendez F; Thompson M; Torres J; Cochet O; Philipson LH. 2010. Calcium-activated and voltage-gated potassium channels of the pancreatic islet impart distinct and complementary roles during secretagogue induced electrical responses. J Physiol 588(Pt 18):3525-37. [PubMed: 20643768] [MGI Ref ID J:177330]
Kilimnik G; Kim A; Jo J; Miller K; Hara M. 2009. Quantification of pancreatic islet distribution in situ in mice. Am J Physiol Endocrinol Metab 297(6):E1331-8. [PubMed: 19808908] [MGI Ref ID J:159564]
Miyatsuka T; Kosaka Y; Kim H; German MS. 2011. Neurogenin3 inhibits proliferation in endocrine progenitors by inducing Cdkn1a. Proc Natl Acad Sci U S A 108(1):185-90. [PubMed: 21173230] [MGI Ref ID J:169013]
Mwangi S; Anitha M; Mallikarjun C; Ding X; Hara M; Parsadanian A; Larsen CP; Thule P; Sitaraman SV; Anania F; Srinivasan S. 2008. Glial cell line-derived neurotrophic factor increases beta-cell mass and improves glucose tolerance. Gastroenterology 134(3):727-37. [PubMed: 18241861] [MGI Ref ID J:135600]
Nyman LR; Ford E; Powers AC; Piston DW. 2010. Glucose-dependent blood flow dynamics in murine pancreatic islets in vivo. Am J Physiol Endocrinol Metab 298(4):E807-14. [PubMed: 20071562] [MGI Ref ID J:162894]
Smukler SR; Arntfield ME; Razavi R; Bikopoulos G; Karpowicz P; Seaberg R; Dai F; Lee S; Ahrens R; Fraser PE; Wheeler MB; van der Kooy D. 2011. The adult mouse and human pancreas contain rare multipotent stem cells that express insulin. Cell Stem Cell 8(3):281-93. [PubMed: 21362568] [MGI Ref ID J:171126]
Yin N; Xu J; Ginhoux F; Randolph GJ; Merad M; Ding Y; Bromberg JS. 2012. Functional specialization of islet dendritic cell subsets. J Immunol 188(10):4921-30. [PubMed: 22508930] [MGI Ref ID J:188667]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice may be bred with wildtype (noncarrier) mice or NOD/ShiLtJ inbred mice (Stock No. 001976). Mating System Noncarrier x Hemizygote (Female x Male) 02-AUG-12 Hemizygote x Noncarrier (Female x Male) 02-AUG-12
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $177.00 Female or Male Hemizygous for Tg(Ins1-EGFP)1Hara
Price per Pair (US dollars $) Pair Genotype $241.00 Hemizygous for Tg(Ins1-EGFP)1Hara x Noncarrier $241.00 Noncarrier x Hemizygous for Tg(Ins1-EGFP)1Hara Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $230.10 Female or Male Hemizygous for Tg(Ins1-EGFP)1Hara
Price per Pair (US dollars $) Pair Genotype $313.30 Hemizygous for Tg(Ins1-EGFP)1Hara x Noncarrier $313.30 Noncarrier x Hemizygous for Tg(Ins1-EGFP)1Hara Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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|
|
Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
- 8/2/12: This strain is ON HOLD for approximately three months for breeding changes to increase productivity.
Control Information
| Control | ||
|---|---|---|
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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