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| These mice possess loxP sites on either side of exon 3 of the Rb1 (retinoblastoma 1) gene. This strain may be useful in cancer or cell cycle/senescence studies. | |||||||||||||||
Type Congenic; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Donating Investigator Tyler Jacks, Massachusetts Institute of Technology Description
These mice possess loxP sites on either side of exon 3 of the targeted gene. Mice that are homozygous for this conditional mutant allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. This strain may be useful in cancer or cell cycle/senescence studies.Development
A targeting vector was designed to introduce a puromycin resistance cassette flanked by loxP sites to intron 3, and an additional loxP site to intron 2. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Transient Cre excision was used to excise the puromycin cassette, leaving exon 3 flanked by loxP sites. This strain was maintained on a mixed background composed of C57BL/6 and 129 by the donating laboratory.
Strains carrying other alleles of Rb1
002082 129S-Rb1tm1Tyj/J 002102 B6.129S2-Rb1tm1Tyj/J 002546 C3Ou.129S2-Rb1tm1Tyj/J 002900 FVB.129S2(B6)-Rb1tm1Tyj/J View Strains carrying other alleles of Rb1 (4 strains)
View Research Applications
Research Applications
This mouse can be used to support research in many areas including:
Cancer Research
Tumor Suppressor Genes
Cell Biology Research
Cell Cycle Regulation
| Allele Symbol | Rb1tm3Tyj | ||
|---|---|---|---|
| Allele Name | targeted mutation 3, Tyler Jacks | ||
| Allele Type | Targeted (Floxed/Frt) | ||
| Common Name(s) | Rb12lox; RbloxP; | ||
| Mutation Made By | Tyler Jacks, Massachusetts Institute of Technology | ||
| Gene Symbol and Name | Rb1, retinoblastoma 1 | ||
| Chromosome | 14 | ||
| Gene Common Name(s) | OSRC; RB; Rb-1; p105-Rb; pRb; pp110; | ||
| Molecular Note | Single loxP sites flank exon 3. [MGI Ref ID J:81643] | ||
Genotyping Protocols
Rb1tm3Tyj, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
Sage J; Miller AL; Perez-Mancera PA; Wysocki JM; Jacks T. 2003. Acute mutation of retinoblastoma gene function is sufficient for cell cycle re-entry. Nature 424(6945):223-8. [PubMed: 12853964] [MGI Ref ID J:84407]
Rb1tm3Tyj relatedBalsitis S; Dick F; Dyson N; Lambert PF. 2006. Critical Roles for Non-pRb Targets of Human Papillomavirus Type 16 E7 in Cervical Carcinogenesis. Cancer Res 66(19):9393-400. [PubMed: 17018593] [MGI Ref ID J:113382]
Balsitis S; Dick F; Lee D; Farrell L; Hyde RK; Griep AE; Dyson N; Lambert PF. 2005. Examination of the pRb-dependent and pRb-independent functions of E7 in vivo. J Virol 79(17):11392-402. [PubMed: 16103190] [MGI Ref ID J:101623]
Balsitis SJ; Sage J; Duensing S; Munger K; Jacks T; Lambert PF. 2003. Recapitulation of the effects of the human papillomavirus type 16 E7 oncogene on mouse epithelium by somatic Rb deletion and detection of pRb-independent effects of E7 in vivo. Mol Cell Biol 23(24):9094-103. [PubMed: 14645521] [MGI Ref ID J:86866]
Berman SD; Calo E; Landman AS; Danielian PS; Miller ES; West JC; Fonhoue BD; Caron A; Bronson R; Bouxsein ML; Mukherjee S; Lees JA. 2008. Metastatic osteosarcoma induced by inactivation of Rb and p53 in the osteoblast lineage. Proc Natl Acad Sci U S A 105(33):11851-6. [PubMed: 18697945] [MGI Ref ID J:139697]
Haigis K; Sage J; Glickman J; Shafer S; Jacks T. 2006. The related retinoblastoma (pRb) and p130 proteins cooperate to regulate homeostasis in the intestinal epithelium. J Biol Chem 281(1):638-47. [PubMed: 16258171] [MGI Ref ID J:106339]
Ho VM; Schaffer BE; Karnezis AN; Park KS; Sage J. 2009. The retinoblastoma gene Rb and its family member p130 suppress lung adenocarcinoma induced by oncogenic K-Ras. Oncogene 28(10):1393-9. [PubMed: 19151761] [MGI Ref ID J:147590]
Ianari A; Natale T; Calo E; Ferretti E; Alesse E; Screpanti I; Haigis K; Gulino A; Lees JA. 2009. Proapoptotic function of the retinoblastoma tumor suppressor protein. Cancer Cell 15(3):184-94. [PubMed: 19249677] [MGI Ref ID J:145939]
Lee EY; Yuan TL; Danielian PS; West JC; Lees JA. 2009. E2F4 cooperates with pRB in the development of extra-embryonic tissues. Dev Biol 332(1):104-15. [PubMed: 19433082] [MGI Ref ID J:152869]
MacPherson D; Conkrite K; Tam M; Mukai S; Mu D; Jacks T. 2007. Murine bilateral retinoblastoma exhibiting rapid-onset, metastatic progression and N-myc gene amplification. EMBO J 26(3):784-94. [PubMed: 17235288] [MGI Ref ID J:119919]
MacPherson D; Sage J; Crowley D; Trumpp A; Bronson RT; Jacks T. 2003. Conditional mutation of Rb causes cell cycle defects without apoptosis in the central nervous system. Mol Cell Biol 23(3):1044-53. [PubMed: 12529408] [MGI Ref ID J:81643]
MacPherson D; Sage J; Kim T; Ho D; McLaughlin ME; Jacks T. 2004. Cell type-specific effects of Rb deletion in the murine retina. Genes Dev 18(14):1681-94. [PubMed: 15231717] [MGI Ref ID J:91406]
Srinivasan SV; Mayhew CN; Schwemberger S; Zagorski W; Knudsen ES. 2007. RB loss promotes aberrant ploidy by deregulating levels and activity of DNA replication factors. J Biol Chem 282(33):23867-77. [PubMed: 17556357] [MGI Ref ID J:124806]
Strati K; Lambert PF. 2007. Role of Rb-dependent and Rb-independent functions of papillomavirus E7 oncogene in head and neck cancer. Cancer Res 67(24):11585-93. [PubMed: 18089787] [MGI Ref ID J:130800]
Viatour P; Somervaille TC; Venkatasubrahmanyam S; Kogan S; McLaughlin ME; Weissman IL; Butte AJ; Passegue E; Sage J. 2008. Hematopoietic stem cell quiescence is maintained by compound contributions of the retinoblastoma gene family. Cell Stem Cell 3(4):416-28. [PubMed: 18940733] [MGI Ref ID J:149791]
Walkley CR; Orkin SH. 2006. Rb is dispensable for self-renewal and multilineage differentiation of adult hematopoietic stem cells. Proc Natl Acad Sci U S A 103(24):9057-62. [PubMed: 16754850] [MGI Ref ID J:111063]
Walkley CR; Qudsi R; Sankaran VG; Perry JA; Gostissa M; Roth SI; Rodda SJ; Snay E; Dunning P; Fahey FH; Alt FW; McMahon AP; Orkin SH. 2008. Conditional mouse osteosarcoma, dependent on p53 loss and potentiated by loss of Rb, mimics the human disease. Genes Dev 22(12):1662-76. [PubMed: 18559481] [MGI Ref ID J:136693]
Colony Maintenance
Breeding & Husbandry When maintained as a live colony, homozygotes or heterozygotes may be bred.
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
| Pricing for International shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Purchasing Information
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Contact Information
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Fax: 1-207-288-6150
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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