Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse   Donating Investigator Tyler Jacks,   Massachusetts Institute of Technology

Description
These mice possess loxP sites on either side of exon 3 of the targeted gene. Mice that are homozygous for this conditional mutant allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. This strain may be useful in cancer or cell cycle/senescence studies.

Development
A targeting vector was designed to introduce a puromycin resistance cassette flanked by loxP sites to intron 3, and an additional loxP site to intron 2. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Transient Cre excision was used to excise the puromycin cassette, leaving exon 3 flanked by loxP sites. This strain was maintained on a mixed background composed of C57BL/6 and 129 by the donating laboratory.

Related Strains

Strains carrying other alleles of Rb1

002082	129S-Rb1tm1Tyj/J
002102	B6.129S2-Rb1tm1Tyj/J
002546	C3Ou.129S2-Rb1tm1Tyj/J
002900	FVB.129S2(B6)-Rb1tm1Tyj/J

View Strains carrying other alleles of Rb1     (4 strains)

Phenotype

Phenotype Information

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cancer Research
Tumor Suppressor Genes

Cell Biology Research
Cell Cycle Regulation

Genes & Alleles

Gene & Allele Information

Allele Symbol		Rb1tm3Tyj
Allele Name		targeted mutation 3, Tyler Jacks
Allele Type		Targeted (Floxed/Frt)
Common Name(s)		Rb12lox; RbloxP;
Mutation Made By		Tyler Jacks,   Massachusetts Institute of Technology
Gene Symbol and Name		Rb1, retinoblastoma 1
Chromosome		14
Gene Common Name(s)		OSRC; RB; Rb-1; p105-Rb; pRb; pp110;
Molecular Note		Single loxP sites flank exon 3. [MGI Ref ID J:81643]

Genotyping

Genotyping Information

Genotyping Protocols

Rb1^tm3Tyj, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Sage J; Miller AL; Perez-Mancera PA; Wysocki JM; Jacks T. 2003. Acute mutation of retinoblastoma gene function is sufficient for cell cycle re-entry. Nature 424(6945):223-8. [PubMed: 12853964]  [MGI Ref ID J:84407]

Additional References

Rb1^tm3Tyj related

Balsitis S; Dick F; Dyson N; Lambert PF. 2006. Critical Roles for Non-pRb Targets of Human Papillomavirus Type 16 E7 in Cervical Carcinogenesis. Cancer Res 66(19):9393-400. [PubMed: 17018593]  [MGI Ref ID J:113382]

Balsitis S; Dick F; Lee D; Farrell L; Hyde RK; Griep AE; Dyson N; Lambert PF. 2005. Examination of the pRb-dependent and pRb-independent functions of E7 in vivo. J Virol 79(17):11392-402. [PubMed: 16103190]  [MGI Ref ID J:101623]

Balsitis SJ; Sage J; Duensing S; Munger K; Jacks T; Lambert PF. 2003. Recapitulation of the effects of the human papillomavirus type 16 E7 oncogene on mouse epithelium by somatic Rb deletion and detection of pRb-independent effects of E7 in vivo. Mol Cell Biol 23(24):9094-103. [PubMed: 14645521]  [MGI Ref ID J:86866]

Berman SD; Calo E; Landman AS; Danielian PS; Miller ES; West JC; Fonhoue BD; Caron A; Bronson R; Bouxsein ML; Mukherjee S; Lees JA. 2008. Metastatic osteosarcoma induced by inactivation of Rb and p53 in the osteoblast lineage. Proc Natl Acad Sci U S A 105(33):11851-6. [PubMed: 18697945]  [MGI Ref ID J:139697]

Haigis K; Sage J; Glickman J; Shafer S; Jacks T. 2006. The related retinoblastoma (pRb) and p130 proteins cooperate to regulate homeostasis in the intestinal epithelium. J Biol Chem 281(1):638-47. [PubMed: 16258171]  [MGI Ref ID J:106339]

Ho VM; Schaffer BE; Karnezis AN; Park KS; Sage J. 2009. The retinoblastoma gene Rb and its family member p130 suppress lung adenocarcinoma induced by oncogenic K-Ras. Oncogene 28(10):1393-9. [PubMed: 19151761]  [MGI Ref ID J:147590]

Ianari A; Natale T; Calo E; Ferretti E; Alesse E; Screpanti I; Haigis K; Gulino A; Lees JA. 2009. Proapoptotic function of the retinoblastoma tumor suppressor protein. Cancer Cell 15(3):184-94. [PubMed: 19249677]  [MGI Ref ID J:145939]

Lee EY; Yuan TL; Danielian PS; West JC; Lees JA. 2009. E2F4 cooperates with pRB in the development of extra-embryonic tissues. Dev Biol 332(1):104-15. [PubMed: 19433082]  [MGI Ref ID J:152869]

MacPherson D; Conkrite K; Tam M; Mukai S; Mu D; Jacks T. 2007. Murine bilateral retinoblastoma exhibiting rapid-onset, metastatic progression and N-myc gene amplification. EMBO J 26(3):784-94. [PubMed: 17235288]  [MGI Ref ID J:119919]

MacPherson D; Sage J; Crowley D; Trumpp A; Bronson RT; Jacks T. 2003. Conditional mutation of Rb causes cell cycle defects without apoptosis in the central nervous system. Mol Cell Biol 23(3):1044-53. [PubMed: 12529408]  [MGI Ref ID J:81643]

MacPherson D; Sage J; Kim T; Ho D; McLaughlin ME; Jacks T. 2004. Cell type-specific effects of Rb deletion in the murine retina. Genes Dev 18(14):1681-94. [PubMed: 15231717]  [MGI Ref ID J:91406]

Srinivasan SV; Mayhew CN; Schwemberger S; Zagorski W; Knudsen ES. 2007. RB loss promotes aberrant ploidy by deregulating levels and activity of DNA replication factors. J Biol Chem 282(33):23867-77. [PubMed: 17556357]  [MGI Ref ID J:124806]

Strati K; Lambert PF. 2007. Role of Rb-dependent and Rb-independent functions of papillomavirus E7 oncogene in head and neck cancer. Cancer Res 67(24):11585-93. [PubMed: 18089787]  [MGI Ref ID J:130800]

Viatour P; Somervaille TC; Venkatasubrahmanyam S; Kogan S; McLaughlin ME; Weissman IL; Butte AJ; Passegue E; Sage J. 2008. Hematopoietic stem cell quiescence is maintained by compound contributions of the retinoblastoma gene family. Cell Stem Cell 3(4):416-28. [PubMed: 18940733]  [MGI Ref ID J:149791]

Walkley CR; Orkin SH. 2006. Rb is dispensable for self-renewal and multilineage differentiation of adult hematopoietic stem cells. Proc Natl Acad Sci U S A 103(24):9057-62. [PubMed: 16754850]  [MGI Ref ID J:111063]

Walkley CR; Qudsi R; Sankaran VG; Perry JA; Gostissa M; Roth SI; Rodda SJ; Snay E; Dunning P; Fahey FH; Alt FW; McMahon AP; Orkin SH. 2008. Conditional mouse osteosarcoma, dependent on p53 loss and potentiated by loss of Rb, mimics the human disease. Genes Dev 22(12):1662-76. [PubMed: 18559481]  [MGI Ref ID J:136693]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	When maintained as a live colony, homozygotes or heterozygotes may be bred.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

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Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location). This strain is included in the Induced Mutant Resource Colony collection.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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