Strain Name: |
STOCK Gata4tm1.1Sad/J |
|---|---|
Stock Number: |
008194 |
Availability: | Under Development for Distribution Colony |
| To register your interest in this strain go to the Strain Interest Form. |
General Terms and Conditions |
| Genes & Alleles | Gata4; Gata4tm1.1Sad; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Mating System Heterozygote x Heterozygote (Female x Male) Species laboratory mouse Donating Investigator Stephen Duncan, Medical College of Wisconsin Generation F?+ (02-SEP-08) Strain Description
Mice homozygous for this Gata4loxP conditional allele are viable and fertile, with loxP sites flanking exons 3-5 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region (coding for both zinc finger DNA-binding domains and the nuclear localization signal essential for GATA4 function) deleted in the cre-expressing tissue(s). These GATA binding protein 4 conditional mice may be useful in generating conditional mutations for studying GATA4 function during organogenesis (such as cardiogenesis) or in adult mice.Strain Development
A targeting vector was designed to place a loxP-flanked thymidine kinase/neomycin phosphotransferase expression (tk/neo) cassette upstream of exon 3, and a third loxP site just downstream of exon 5 of the targeted gene. The targeting construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a cre expressing plasmid to remove the selection cassette (generating the Gata4loxP allele; with one loxP site upstream of exon 3 and one loxP site downstream of exon 5). These ES cells were combined with CD1 morula to generate chimeric animals, which were then bred with CD1 mice. These Gata4loxP mice were maintained on a mixed genetic background prior to arrival at The Jackson Laboratory. Upon arrival, mice may have been bred to 129S1/SvImJ for at least one generation to establish the colony.
Gene & Allele Details
| Allele Symbol | Gata4tm1.1Sad | ||
|---|---|---|---|
| Allele Name | targeted mutation 1.1, Stephen A Duncan | ||
| Common Name(s) | Gata4loxP; | ||
| Mutation Made By | Stephen Duncan, Medical College of Wisconsin | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Gene Symbol and Name | Gata4, GATA binding protein 4 | ||
| Chromosome | 14 | ||
| Gene Common Name(s) | Gata-4; MGC126629; | ||
| Molecular Note | A targeting vector was designed to place a loxP-flanked thymidine kinase/neomycin phosphotransferase expression (tk/neo) cassette upstream of exon 3, and a third loxP site just downstream of exon 5 of the targeted gene. Correctly targeted ES cells were transiently transfected with a cre expressing plasmid to remove the selection cassette (generating the Gata4-loxP allele; with one loxP site upstream of exon 3 and one loxP site downstream of exon 5). (details provided by SA Duncan, personal communication.) [MGI Ref ID J:92434] | ||
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Gata4tm1.1Sad
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, homozygous mice may be bred together. |
|---|
Related Strains
Strains carrying other alleles of Gata4
006807 B6Ei.129S6(Cg)-Gata4tm1.1Sho/EiJ 006808 D2.129S6(Cg)-Gata4tm1.1Sho/EiJ View Strains carrying other alleles of Gata4 (2 strains)
Additional Web Information
Cre-lox or FLP-FRT Systems
Research Applications
This mouse can be used to support research in many areas including:
Research Tools
Cardiovascular Research (Cre-lox System)
Cre-lox System (loxP-flanked Sequences)
Developmental Biology Research (Cre-lox System)
References
Selected Reference(s)
Additional ReferencesWatt AJ; Battle MA; Li J; Duncan SA. 2004. GATA4 is essential for formation of the proepicardium and regulates cardiogenesis. Proc Natl Acad Sci U S A 101(34):12573-8. [PubMed: 15310850] [MGI Ref ID J:92434]
Price and Supply Information
| Strain Name: | STOCK Gata4tm1.1Sad/J |
| Stock Number: | 008194 |
This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date: 06-OCT-08
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
View All Strains Under Development
Supply Details
| Standard Supply | Under Development for Distribution Colony |
|---|---|
| Supply Notes |
This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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