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Genes & Alleles

Adipoq;   Adipoqtm1Chan;

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Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Donating Investigator Lawrence Chan,   Baylor College of Medicine

Strain Description
Homozygous mice are viable and fertile, with absence of targeted allele expression confirmed in adipose tissue (mRNA) and plasma (adiponectin protein). While homozygous mice have normal glucose tolerance and insulin resistance, beta-oxidation activity is significantly increased in muscle and liver. Homozygotes also have endothelial dysfunction (increased leukocyte rolling and leukocyte adhesion), are protected from DSS-induced colitis, and are more susceptible to myocardial ischemia/reperfusion. When fed a high fat diet, obese homozygotes are significantly heavier with increased insulin levels and altered insulin resistance. These adiponectin-deficient (Adipoq^-/- or Adipo^-/-) mice may be useful in studying obesity, diabetes, insulin resistance, metabolism, inflammation, leukocyte-endothelium interactions, and colitis.

Strain Development
A targeting vector was designed to replace exon 2 of the targeted gene with a PGKneo cassette. This construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and chimeric mice were bred with C57BL/6J to generate mutant mice. Next, mutant mice were backcrossed to C57BL/6J for at least 8 generations prior to arrival at The Jackson Laboratory.

Gene & Allele Details

Allele Symbol		Adipoqtm1Chan
Allele Name		targeted mutation 1, Lawrence Chan
Common Name(s)		Ad-; Adipo-;
Mutation Made By		Lawrence Chan,   Baylor College of Medicine
Strain of Origin		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line Name		R1
ES Cell Line Strain		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name		Adipoq, adiponectin, C1Q and collagen domain containing
Chromosome		16
Gene Common Name(s)		ACDC; ACRP30; ADPN; APM-1; APM1; APN; Acdc; Acrp30; GBP28; adipo; adipocyte complement related protein; adipocyte, C1Q and collagen domain containing; adiponectin;
Molecular Note		Exon 2 was replaced by a floxed neo cassette inserted by homologous recombination. Transcript was undetected in adipose tissue by Northern blot analysis of homozygous mutant mice. The lack of immunoreactivity by Western blot analysis using a carboxy terminal antibody indicated an absence of normal and truncated protein. [MGI Ref ID J:79122]

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, homozygous mice may be bred.

Additional Web Information

Congenic Nomenclature

Research Applications

This mouse can be used to support research in many areas including:

Cardiovascular Research
Ischemia studies
Vascular Defects (defective leukocyte function)

Diabetes and Obesity Research
Insulin Resistance (diet-induced)
Obesity With Diabetes (diet-induced)
Obesity Without Diabetes (diet-induced)
Type 2 Diabetes (NIDDM) (diet-induced)

Immunology and Inflammation Research
Autoimmunity
Immunodeficiency (Inflammatory bowel disease)
Inflammation (Inflammatory bowel disease)

Internal/Organ Research
Gastrointestinal Defects (colitis)
Wound Healing

Metabolism Research
Free Radical Research

Research Tools
Cardiovascular Research
Diabetes and Obesity Research
Immunology and Inflammation Research
Internal/Organ Research
Metabolism Research

References

Selected Reference(s)

Fayad R; Pini M; Sennello JA; Cabay RJ; Chan L; Xu A; Fantuzzi G. 2007. Adiponectin deficiency protects mice from chemically induced colonic inflammation. Gastroenterology 132(2):601-14. [PubMed: 17258715]  [MGI Ref ID J:128222]

Ma K; Cabrero A; Saha PK; Kojima H; Li L; Chang BH; Paul A; Chan L. 2002. Increased beta -oxidation but no insulin resistance or glucose intolerance in mice lacking adiponectin. J Biol Chem 277(38):34658-61. [PubMed: 12151381]  [MGI Ref ID J:79122]

Ouedraogo R; Gong Y; Berzins B; Wu X; Mahadev K; Hough K; Chan L; Goldstein BJ; Scalia R. 2007. Adiponectin deficiency increases leukocyte-endothelium interactions via upregulation of endothelial cell adhesion molecules in vivo. J Clin Invest 117(6):1718-26. [PubMed: 17549259]  [MGI Ref ID J:122020]

Tao L; Gao E; Jiao X; Yuan Y; Li S; Christopher TA; Lopez BL; Koch W; Chan L; Goldstein BJ; Ma XL. 2007. Adiponectin cardioprotection after myocardial ischemia/reperfusion involves the reduction of oxidative/nitrative stress. Circulation 115(11):1408-16. [PubMed: 17339545]  [MGI Ref ID J:133046]

Wu H; Ghosh S; Perrard XD; Feng L; Garcia GE; Perrard JL; Sweeney JF; Peterson LE; Chan L; Smith CW; Ballantyne CM. 2007. T-cell accumulation and regulated on activation, normal T cell expressed and secreted upregulation in adipose tissue in obesity. Circulation 115(8):1029-38. [PubMed: 17296858]  [MGI Ref ID J:132316]

Additional References

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Price and Supply Information

Strain Name:	B6.129-Adipoqtm1Chan/J
Stock Number:	008195

 

This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.

Estimated Available for Sale Date:

Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.

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Supply Details

Standard Supply	Under Development for Distribution Colony
Supply Notes	This strain is included in the Induced Mutant Resource Colony collection.
Licensing	See General Terms and Conditions below for Licensing and Use Restrictions
Control Information	View Control Information in Strain Details.

General Terms and Conditions

View JAX^® Mice & Services Conditions of Use.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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