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Genes & Alleles

Gata6;   Gata6tm2.1Sad;

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Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Mating System Heterozygote x Heterozygote         (Female x Male) Species laboratory mouse Donating Investigator Stephen Duncan,   Medical College of Wisconsin

Strain Description
Mice homozygous for this Gata6^loxp conditional allele are viable and fertile, with loxP sites flanking exon 2 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region (coding for the majority of the GATA6 protein) deleted in the cre-expressing tissue(s). These Gata6^loxp mice may be useful in generating conditional GATA binding protein 6 mutations for studying GATA6 function in organogenesis or in adult mice.

For example, when bred to a strain expressing Cre recombinase in the villi and crypt cells of the intestine (see Stock No. 004586 for example), or when bred to a strain expressing Cre recombinase in the embryo (see Stock No. 003724, 003314 for example), this mutant mouse strain may be useful in studies of the transcription factor Gata6.

Strain Development
A targeting vector was designed to place a loxP site and frt-flanked neo cassette upstream of exon 2, and a second loxP site just downstream of exon 2 of the targeted gene. The targeting construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Chimeric mice were bred with CD-1 mice. To remove the selection cassette, these Gata6^{loxp(FRTneoFRT)} mice were bred to FLPe-expressing mice (on a B6;SJL genetic background, see Stock No. 003800). The resulting Gata6^loxp offspring were then bred to CD-1 mice to remove the transgene. These Gata6^loxp mice were maintained on a mixed genetic background prior to arrival at The Jackson Laboratory. Upon arrival, mice may have been bred to 129S1/SvImJ for at least one generation to establish the colony.

Mammalian Phenotype Terms assigned by genotype Gata6tm2.1Sad/Gata6tm2.1Sad         involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CD-1 * SJL normal phenotype no abnormal phenotype detected (MGI Ref ID J:111388) viable and fertile with no gross malformations The following phenotype relates to a compound genotype created using this strain. Contact JAX® Services jaxservices@jax.org for customized breeding options. Gata6tm2.1Sad/Gata6tm2.1Sad Tg(Vil-cre)997Gum/0         involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CD-1 * SJL   (conditional) lethality-prenatal/perinatal embryonic lethality during organogenesis (MGI Ref ID J:111388) matings of 4 of 5 males carrying the Cre transgene to homozygous females produced no offspring homozygous for the floxed allele and Cre positive Gata6tm2.1Sad/Gata6tm2.2Sad Tg(EIIa-cre)C5379Lmgd/0         involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CD-1 * FVB * SJL   (conditional) lethality-prenatal/perinatal embryonic lethality during organogenesis (MGI Ref ID J:111388) between E8.5 and E11.5 all Cre positive double mutants appear to be partially resorbed empty decidual masses

Gene & Allele Details

Allele Symbol		Gata6tm2.1Sad
Allele Name		targeted mutation 2.1, Stephen A Duncan
Common Name(s)		Gata6 "floxed"; Gata6loxP;
Mutation Made By		Stephen Duncan,   Medical College of Wisconsin
Strain of Origin		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line Name		R1
ES Cell Line Strain		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name		Gata6, GATA binding protein 6
Chromosome		18
Gene Common Name(s)		AA410133; expressed sequence AA410133;
General Note		This allele was derived by deletion of an FRT-flanked neomycin phosphotransferase gene that in the original construct lay just downstream of the 5' loxP site. Mice homozygous for the neo-containing allele could not be recovered from heterozygous matings.(J:111388)
Molecular Note		A pair of loxP sequences are located in the first and second introns, flanking exon 2. A single FRT sequence is present immediately downstream of the more 5' loxP as a consequence of the removal of an frt-flanked neomycin seletion cassette. [MGI Ref ID J:111388]

Control Information

	Control
	Wild-type from the colony
Considerations for Choosing Controls

Genotyping Protocols

Gata6^tm2.1Sad

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, homozygous mice may be bred together.
Diet Information	LabDiet® 5K52/5K67

Additional Web Information

Cre-lox or FLP-FRT Systems

Research Applications

This mouse can be used to support research in many areas including:

Research Tools
Cardiovascular Research (Cre-lox System)
Cre-lox System (loxP-flanked Sequences)
Developmental Biology Research (Cre-lox System)

References

Selected Reference(s)

Sodhi CP; Li J; Duncan SA. 2006. Generation of mice harbouring a conditional loss-of-function allele of Gata6. BMC Dev Biol 6:19. [PubMed: 16611361]  [MGI Ref ID J:111388]

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Price and Supply Information

Strain Name:	STOCK Gata6tm2.1Sad/J
Stock Number:	008196

 

This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.

Estimated Available for Sale Date: 13-OCT-08

Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.

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Supply Details

Standard Supply	Under Development for Distribution Colony
Supply Notes	This strain is included in the Induced Mutant Resource Colony collection.
Licensing	See General Terms and Conditions below
Control Information	View Control Information in Strain Details.

General Terms and Conditions

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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