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| These Dlx5/6-Cre transgenic mice have Cre recombinase expression directed by the regulatory sequences of the zebrafish dlx5a/dlx6a genes and may be useful in generating specific deletions of floxed alleles in GABAergic forebrain neurons. | |||||||||||||||
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Description
Strain Information
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Noncarrier x Hemizygote (Female x Male) 31-MAR-09 Species laboratory mouse Generation F?+F10 (13-OCT-11)
Generation DefinitionsDonating Investigator Marc Ekker, University of Ottawa Description
Homozygous Dlx5/6-Cre transgenic mice are viable and fertile. Expression of Cre recombinase (Cre) is directed to differentiating and migrating forebrain GABAergic neurons during embryonic development by the I56i and I56ii enhancers from the zebrafish dlx5a/dlx6a intergenic region (with the 5' promoter region of zebrafish dlx6a in place to increase the activity of the intergenic enhancers rather than direct tissue-specific expression). When Dlx5/6-Cre transgenic mice are bred with mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of the floxed sequence in the offspring in the offspring. These Dlx5/6-Cre transgenic mice may be useful in generating specific deletions of floxed alleles in GABAergic forebrain neurons.Development
The Dlx5/6-Cre transgene was designed with a 3.5 kb fragment from the immediate 5'-flanking region of zebrafish dlx6a gene (including part of the 5' UTR) placed immediately upstream of a Cre recombinase coding sequence, all followed by a 1.4 kb enhancer fragment from the zebrafish dlx5a/dlx6a intergenic region (containing both zebrafish I56i and I56ii enhancers). The donating investigator reports that a female founder mouse (founder #8561 on a CD1 genetic background) was bred to CD1 males and the resulting Dlx5/6-Cre mice were maintained by breeding hemizygous males with CD1 females for at least 10 generations prior to arrival at The Jackson Laboratory Repository. Upon arrival, transgenic animals were bred with C57BL/6J mice for at least one generation to establish the colony.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of cre
View Strains carrying other alleles of cre (406 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedNeurobiology Research
Cre-lox System
Cre Recombinase expression in neural tissue
Research Tools
Cre-lox System
Cre Recombinase Expression
Developmental Biology Research
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Tissue/Cell Markers: neurons
Neurobiology Research
cell marker
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(dlx6a-cre)1Mekk | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Marc Ekker | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | Dlx5/6-Cre; Dlx5/6i-Cre; Tg(dlx6a-cre)1Mars; line 8561; | ||
| Mutation Made By | Marc Ekker, University of Ottawa | ||
| Strain of Origin | CD-1 | ||
| Site of Expression | GABAergic forebrain neurons | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | dlx6a, distal-less homeobox gene 6a, Danio rerio, | ||
| Driver Note | dlx6a | ||
| Molecular Note | A cre gene was placed under the control of the I56i and I56ii enhancer elements of zebrafish dlx5a and a 3.5kb genomic DNA fragment flanking the 5' region of the zebrafish dlx6a open reading frame and containing the 5'UTR. The dlx6a flanking region on its own cannot produce tissue specific expression but increases the activity of the enhancers to produce expression that recapitulate endogenous Dlx5 expression. Tissue specific expression was confirmed by test crosses to reporter mice strains, including Tg(ACTB-Bgeo/ALPP)1Lbe, Tg(ACTB-Bgeo/GFP)21Lbe and Gt(ROSA)26Sortm1Sor. [MGI Ref ID J:122066] | ||
| Gene Symbol and Name | Tg(dlx6a-cre)1Mekk, transgene insertion 1, Marc Ekker | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Dlx5/6-Cre; Tg(dlx6a-cre)1Mars; transgene insertion 1, Giovanni Marsicano; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Monory K; Massa F; Egertova M; Eder M; Blaudzun H; Westenbroek R; Kelsch W; Jacob W; Marsch R; Ekker M; Long J; Rubenstein JL; Goebbels S; Nave KA; During M; Klugmann M; Wolfel B; Dodt HU; Zieglgansberger W; Wotjak CT; Mackie K; Elphick MR; Marsicano G; Lutz B. 2006. The endocannabinoid system controls key epileptogenic circuits in the hippocampus. Neuron 51(4):455-66. [PubMed: 16908411] [MGI Ref ID J:122066]
Additional References
Tg(dlx6a-cre)1Mekk relatedBellocchio L; Lafenetre P; Cannich A; Cota D; Puente N; Grandes P; Chaouloff F; Piazza PV; Marsicano G. 2010. Bimodal control of stimulated food intake by the endocannabinoid system. Nat Neurosci 13(3):281-3. [PubMed: 20139974] [MGI Ref ID J:158629]
Benard G; Massa F; Puente N; Lourenco J; Bellocchio L; Soria-Gomez E; Matias I; Delamarre A; Metna-Laurent M; Cannich A; Hebert-Chatelain E; Mulle C; Ortega-Gutierrez S; Martin-Fontecha M; Klugmann M; Guggenhuber S; Lutz B; Gertsch J; Chaouloff F; Lopez-Rodriguez ML; Grandes P; Rossignol R; Marsicano G. 2012. Mitochondrial CB(1) receptors regulate neuronal energy metabolism. Nat Neurosci 15(4):558-64. [PubMed: 22388959] [MGI Ref ID J:191260]
Berghuis P; Rajnicek AM; Morozov YM; Ross RA; Mulder J; Urban GM; Monory K; Marsicano G; Matteoli M; Canty A; Irving AJ; Katona I; Yanagawa Y; Rakic P; Lutz B; Mackie K; Harkany T. 2007. Hardwiring the brain: endocannabinoids shape neuronal connectivity. Science 316(5828):1212-6. [PubMed: 17525344] [MGI Ref ID J:121856]
Chao HT; Chen H; Samaco RC; Xue M; Chahrour M; Yoo J; Neul JL; Gong S; Lu HC; Heintz N; Ekker M; Rubenstein JL; Noebels JL; Rosenmund C; Zoghbi HY. 2010. Dysfunction in GABA signalling mediates autism-like stereotypies and Rett syndrome phenotypes. Nature 468(7321):263-9. [PubMed: 21068835] [MGI Ref ID J:166851]
Diaz-Alonso J; Aguado T; Wu CS; Palazuelos J; Hofmann C; Garcez P; Guillemot F; Lu HC; Lutz B; Guzman M; Galve-Roperh I. 2012. The CB(1) cannabinoid receptor drives corticospinal motor neuron differentiation through the Ctip2/Satb2 transcriptional regulation axis. J Neurosci 32(47):16651-65. [PubMed: 23175820] [MGI Ref ID J:192580]
Genetic Resource Sciences at The Jackson Laboratory. 2013. Expression/Specificity Patterns of Cre Alleles, 2013 MGI Direct Data Submission :. [MGI Ref ID J:193672]
Haring M; Grieb M; Monory K; Lutz B; Moreira FA. 2013. Cannabinoid CB(1) receptor in the modulation of stress coping behavior in mice: the role of serotonin and different forebrain neuronal subpopulations. Neuropharmacology 65:83-9. [PubMed: 23000076] [MGI Ref ID J:192754]
Hays SA; Huber KM; Gibson JR. 2011. Altered Neocortical Rhythmic Activity States in Fmr1 KO Mice Are Due to Enhanced mGluR5 Signaling and Involve Changes in Excitatory Circuitry. J Neurosci 31(40):14223-34. [PubMed: 21976507] [MGI Ref ID J:177183]
Kohwi M; Petryniak MA; Long JE; Ekker M; Obata K; Yanagawa Y; Rubenstein JL; Alvarez-Buylla A. 2007. A subpopulation of olfactory bulb GABAergic interneurons is derived from Emx1- and Dlx5/6-expressing progenitors. J Neurosci 27(26):6878-91. [PubMed: 17596436] [MGI Ref ID J:160206]
Kuhne C; Puk O; Graw J; Hrabe de Angelis M; Schutz G; Wurst W; Deussing JM. 2012. Visualizing corticotropin-releasing hormone receptor type 1 expression and neuronal connectivities in the mouse using a novel multifunctional allele. J Comp Neurol 520(14):3150-80. [PubMed: 22886732] [MGI Ref ID J:187372]
Li G; Adesnik H; Li J; Long J; Nicoll RA; Rubenstein JL; Pleasure SJ. 2008. Regional distribution of cortical interneurons and development of inhibitory tone are regulated by Cxcl12/Cxcr4 signaling. J Neurosci 28(5):1085-98. [PubMed: 18234887] [MGI Ref ID J:131835]
Li Y; Hibbs MA; Gard AL; Shylo NA; Yun K. 2012. Genome-wide analysis of N1ICD/RBPJ targets in vivo reveals direct transcriptional regulation of Wnt, SHH, and hippo pathway effectors by Notch1. Stem Cells 30(4):741-52. [PubMed: 22232070] [MGI Ref ID J:190509]
Li Y; Yui D; Luikart BW; McKay RM; Li Y; Rubenstein JL; Parada LF. 2012. Conditional ablation of brain-derived neurotrophic factor-TrkB signaling impairs striatal neuron development. Proc Natl Acad Sci U S A 109(38):15491-6. [PubMed: 22949667] [MGI Ref ID J:190151]
Lourenco J; Cannich A; Carta M; Coussen F; Mulle C; Marsicano G. 2010. Synaptic activation of kainate receptors gates presynaptic CB(1) signaling at GABAergic synapses. Nat Neurosci 13(2):197-204. [PubMed: 20081851] [MGI Ref ID J:156676]
Lu A; Steiner MA; Whittle N; Vogl AM; Walser SM; Ableitner M; Refojo D; Ekker M; Rubenstein JL; Stalla GK; Singewald N; Holsboer F; Wotjak CT; Wurst W; Deussing JM. 2008. Conditional mouse mutants highlight mechanisms of corticotropin-releasing hormone effects on stress-coping behavior. Mol Psychiatry 13(11):1028-42. [PubMed: 18475271] [MGI Ref ID J:149207]
Massa F; Mancini G; Schmidt H; Steindel F; Mackie K; Angioni C; Oliet SH; Geisslinger G; Lutz B. 2010. Alterations in the hippocampal endocannabinoid system in diet-induced obese mice. J Neurosci 30(18):6273-81. [PubMed: 20445053] [MGI Ref ID J:160561]
Metna-Laurent M; Soria-Gomez E; Verrier D; Conforzi M; Jego P; Lafenetre P; Marsicano G. 2012. Bimodal Control of Fear-Coping Strategies by CB1 Cannabinoid Receptors. J Neurosci 32(21):7109-18. [PubMed: 22623656] [MGI Ref ID J:184981]
Monory K; Blaudzun H; Massa F; Kaiser N; Lemberger T; Schutz G; Wotjak CT; Lutz B; Marsicano G. 2007. Genetic dissection of behavioural and autonomic effects of Delta(9)-tetrahydrocannabinol in mice. PLoS Biol 5(10):e269. [PubMed: 17927447] [MGI Ref ID J:128453]
Narboux-Neme N; Goiame R; Mattei MG; Cohen-Tannoudji M; Wassef M. 2012. Integration of H-2Z1, a Somatosensory Cortex-Expressed Transgene, Interferes with the Expression of the Satb1 and Tbc1d5 Flanking Genes and Affects the Differentiation of a Subset of Cortical Interneurons. J Neurosci 32(21):7287-7300. [PubMed: 22623674] [MGI Ref ID J:184976]
Ohtsuka N; Tansky MF; Kuang H; Kourrich S; Thomas MJ; Rubenstein JL; Ekker M; Leeman SE; Tsien JZ. 2008. Functional disturbances in the striatum by region-specific ablation of NMDA receptors. Proc Natl Acad Sci U S A 105(35):12961-6. [PubMed: 18728179] [MGI Ref ID J:139133]
Refojo D; Schweizer M; Kuehne C; Ehrenberg S; Thoeringer C; Vogl AM; Dedic N; Schumacher M; von Wolff G; Avrabos C; Touma C; Engblom D; Schutz G; Nave KA; Eder M; Wotjak CT; Sillaber I; Holsboer F; Wurst W; Deussing JM. 2011. Glutamatergic and dopaminergic neurons mediate anxiogenic and anxiolytic effects of CRHR1. Science 333(6051):1903-7. [PubMed: 21885734] [MGI Ref ID J:176339]
Sanchez-Ortiz E; Yui D; Song D; Li Y; Rubenstein JL; Reichardt LF; Parada LF. 2012. TrkA gene ablation in basal forebrain results in dysfunction of the cholinergic circuitry. J Neurosci 32(12):4065-79. [PubMed: 22442072] [MGI Ref ID J:183467]
Shen HY; Coelho JE; Ohtsuka N; Canas PM; Day YJ; Huang QY; Rebola N; Yu L; Boison D; Cunha RA; Linden J; Tsien JZ; Chen JF. 2008. A critical role of the adenosine A2A receptor in extrastriatal neurons in modulating psychomotor activity as revealed by opposite phenotypes of striatum and forebrain A2A receptor knock-outs. J Neurosci 28(12):2970-5. [PubMed: 18354001] [MGI Ref ID J:133212]
Steindel F; Lerner R; Haring M; Ruehle S; Marsicano G; Lutz B; Monory K. 2013. Neuron-type specific cannabinoid-mediated G protein signalling in mouse hippocampus. J Neurochem 124(6):795-807. [PubMed: 23289830] [MGI Ref ID J:193914]
Yee CL; Wang Y; Anderson S; Ekker M; Rubenstein JL. 2009. Arcuate nucleus expression of NKX2.1 and DLX and lineages expressing these transcription factors in neuropeptide Y(+), proopiomelanocortin(+), and tyrosine hydroxylase(+) neurons in neonatal and adult mice. J Comp Neurol 517(1):37-50. [PubMed: 19711380] [MGI Ref ID J:161578]
Yu C; Gupta J; Chen JF; Yin HH. 2009. Genetic deletion of A2A adenosine receptors in the striatum selectively impairs habit formation. J Neurosci 29(48):15100-3. [PubMed: 19955361] [MGI Ref ID J:158458]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number MGL375
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, the donating investigator breeds hemizygotes with noncarrier (wildtype) siblings. Mating System Noncarrier x Hemizygote (Female x Male) 31-MAR-09 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Hemizygous for Tg(dlx6a-cre)1Mekk
Price per Pair (US dollars $) Pair Genotype $302.00 Hemizygous for Tg(dlx6a-cre)1Mekk x Noncarrier $302.00 Noncarrier x Hemizygous for Tg(dlx6a-cre)1Mekk Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Hemizygous for Tg(dlx6a-cre)1Mekk
Price per Pair (US dollars $) Pair Genotype $392.60 Hemizygous for Tg(dlx6a-cre)1Mekk x Noncarrier $392.60 Noncarrier x Hemizygous for Tg(dlx6a-cre)1Mekk Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
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|
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Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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