Strain Name:

FVB/N-Tg(NPHS2-rtTA2*M2)1Jbk/J

Stock Number:

008202

Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
These podocin-rtTA mice provide a Tet-On tool that allows dox-inducible expression of genes in podocytes, and may be useful in studying the role of podocyte nephrobiology in renal disorders.

Description

Strain Information

Type Coisogenic; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
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Mating SystemNoncarrier x Hemizygote         (Female x Male)
Specieslaboratory mouse
GenerationF?+N1F2 (18-DEC-08)
 
Donating Investigator Jeffrey Kopp,   NIDDK, NIH (AMDCC)

Description
These mice express an optimized reverse tetracycline-controlled transactivator (rtTA2-M2) protein under the control of the human NPHS2 promoter, which directs expression to podocytes within kidney glomeruli. When mated to a second strain carrying a gene under the regulatory control of a tetracycline-responsive promoter element (TRE or tetO), expression of the gene in podocytes is induced with administration of the tetracycline analog, doxycycline (dox). These podocin-rtTA mice provide a Tet-On tool that allows dox-inducible expression of genes in podocytes, and may be useful in studying the role of podocyte nephrobiology in renal disorders.

Development
The podocin-rtTA (or NPHS2-rtTA) construct was designed with a 2.5 kb fragment of the podocin (or NPHS2) promoter/enhancer region upstream of an optimized reverse tetracycline-controlled transactivator variant (rtTA2-M2; modified with humanized codon usage and the FFF minimal activator domains), followed by an SV40 3' UTR and polyA region. This transgene was microinjected into the pronuclei of fertilized FVB/N oocytes. Founder mice were bred to FVB/N inbred mice. This strain was maintained on the FVB/N genetic background prior to arrival at The Jackson Laboratory. Upon arrival, mice may have been bred to FVB/NJ inbred mice (Stock No. 001800) to establish the colony.

Control Information

  Control
   Noncarrier
   001800 FVB/NJ
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of NPHS2
008205   B6.Cg-Tg(NPHS2-cre)295Lbh/J
View Strains carrying other alleles of NPHS2     (1 strain)

View Strains carrying other alleles of rtTA     (20 strains)

Additional Web Information

Tet Expression Systems

Phenotype

Phenotype Information

Currently there is no phenotype information for this strain.

Genes & Alleles

Gene & Allele Information

 
Allele Symbol Tg(NPHS2-rtTA2*M2)1Jbk
Allele Name transgene insertion 1, Jeffrey B Kopp
Allele Type Transgenic (random, expressed)
Common Name(s) Tg(NPHS2-rtTA)1Jbk; podocin-rtTA;
Mutation Made By Jeffrey Kopp,   NIDDK, NIH (AMDCC)
Strain of OriginFVB/N
Site of Expressionpodocytes within kidney glomeruli
Expressed Gene rtTA, reverse tetracycline-controlled transactivator, E. coli
The tetracycline repressor gene (Tetr), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). One mutant with a four amino acid residue change (rTetR) exhibited dependence on tetracycline for induction of the targeted gene and was used in the rtTA construct (Gossen et al, 1995). rTetr was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16).
Promoter NPHS2, nephrosis 2, idiopathic, steroid-resistant (podocin), human
Molecular Note The rtTA gene was placed under the control of the human NPHS2 gene. The resulting transgene is expressed in the kidney when induced by doxycycline. [MGI Ref ID J:109826]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(NPHS2-rtTA2*M2)1Jbk, STD PCR, vers. 1

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Shigehara T; Zaragoza C; Kitiyakara C; Takahashi H; Lu H; Moeller M; Holzman LB; Kopp JB. 2003. Inducible podocyte-specific gene expression in transgenic mice. J Am Soc Nephrol 14(8):1998-2003. [PubMed: 12874453]  [MGI Ref ID J:109826]

Additional References

Tg(NPHS2-rtTA2*M2)1Jbk related

Davis B; Dei Cas A; Long DA; White KE; Hayward A; Ku CH; Woolf AS; Bilous R; Viberti G; Gnudi L. 2007. Podocyte-specific expression of angiopoietin-2 causes proteinuria and apoptosis of glomerular endothelia. J Am Soc Nephrol 18(8):2320-9. [PubMed: 17625119]  [MGI Ref ID J:148150]

Ku CH; White KE; Dei Cas A; Hayward A; Webster Z; Bilous R; Marshall S; Viberti G; Gnudi L. 2008. Inducible overexpression of sFlt-1 in podocytes ameliorates glomerulopathy in diabetic mice. Diabetes 57(10):2824-33. [PubMed: 18647955]  [MGI Ref ID J:141994]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX11

Colony Maintenance

Breeding & HusbandryWhen maintaining the live congenic colony, hemizygous mice are bred with wildtype siblings or to FVB/NJ inbred mice.
Mating SystemNoncarrier x Hemizygote         (Female x Male)
Diet Information LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Weeks of AgePrice (US dollars $)GenderGenotypes Provided
Individual Mouse $243.50Female or MaleHemizygous for Tg(NPHS2-rtTA2*M2)1Jbk
Pairs /Price (US dollars $)Pair Genotype
$297.85Hemizygous for Tg(NPHS2-rtTA2*M2)1Jbk x Noncarrier
$297.85Noncarrier x Hemizygous for Tg(NPHS2-rtTA2*M2)1Jbk

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Weeks of AgePrice (US dollars $)GenderGenotypes Provided
Individual Mouse $316.60Female or MaleHemizygous for Tg(NPHS2-rtTA2*M2)1Jbk
Pairs /Price (US dollars $)Pair Genotype
$387.30Hemizygous for Tg(NPHS2-rtTA2*M2)1Jbk x Noncarrier
$387.30Noncarrier x Hemizygous for Tg(NPHS2-rtTA2*M2)1Jbk

Additional Supply Details

Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement.
Supply Notes

Control Information

  Control
   Noncarrier
   001800 FVB/NJ
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

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