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| These podocin-rtTA mice provide a Tet-On tool that allows dox-inducible expression of genes in podocytes, and may be useful in studying the role of podocyte nephrobiology in renal disorders. | |||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Coisogenic; Transgenic; Additional information on Genetically Engineered Mutant Mice. Mating System Noncarrier x Hemizygote (Female x Male) Species laboratory mouse Generation F?+N1 (18-JUL-08) Donating Investigator Jeffrey Kopp, NIDDK, NIH (AMDCC) Description
These mice express an optimized reverse tetracycline-controlled transactivator (rtTA2-M2) protein under the control of the human NPHS2 promoter, which directs expression to podocytes within kidney glomeruli. When mated to a second strain carrying a gene under the regulatory control of a tetracycline-responsive promoter element (TRE or tetO), expression of the gene in podocytes is induced with administration of the tetracycline analog, doxycycline (dox). These podocin-rtTA mice provide a Tet-On tool that allows dox-inducible expression of genes in podocytes, and may be useful in studying the role of podocyte nephrobiology in renal disorders.Development
The podocin-rtTA (or NPHS2-rtTA) construct was designed with a 2.5 kb fragment of the podocin (or NPHS2) promoter/enhancer region upstream of an optimized reverse tetracycline-controlled transactivator variant (rtTA2-M2; modified with humanized codon usage and the FFF minimal activator domains), followed by an SV40 3' UTR and polyA region. This transgene was microinjected into the pronuclei of fertilized FVB/N oocytes. Founder mice were bred to FVB/N inbred mice. This strain was maintained on the FVB/N genetic background prior to arrival at The Jackson Laboratory. Upon arrival, mice may have been bred to FVB/NJ inbred mice (Stock No. 001800) to establish the colony.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of rtTA
005670 B6.Cg-Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J 006235 B6.Cg-Tg(SFTPC-rtTA)5Jaw/J 006232 B6.Cg-Tg(Scgb1a1-rtTA)1Jaw/J 007678 B6;SJL-Tg(KRT14-rtTA)208Jek/J 006245 C.Cg-Tg(SFTPC-rtTA)5Jaw/J 006242 C.Cg-Tg(Scgb1a1-rtTA)1Jaw/J 008099 FVB-Tg(KRT14-rtTA)F42Efu/J 004127 FVB-Tg(Nes-rtTA)306Rvs/J 006225 FVB.Cg-Tg(SFTPC-rtTA)5Jaw/J 006222 FVB.Cg-Tg(Scgb1a1-rtTA)1Jaw/J 006875 FVB/N-Tg(Tagln-rtTA)E1Jwst/J 004602 NOD.Cg-Tg(Ins2-rtTA)2Doi/DoiJ 005734 NOD/Lt-Tg(Ins2-rtTA)1Ach/AchJ 005572 STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J 008250 STOCK Tg(Ins2-rtTA)2Efr/J 005493 STOCK Tg(Tek-rtTA,TRE-lacZ)1425Tpr/J 003273 STOCK Tg(rtTAhCMV)4Bjd/J View Strains carrying other alleles of rtTA (17 strains)
Additional Web Information
Tet Expression Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Research Tools
Genetics Research (Mutagenesis and Transgenesis: Tetop Tet System)
Genetics Research (Tissue/Cell Markers: kidney specific marker)
Tet Expression Systems (tTA/rtTA Expressing Strains)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(NPHS2-rtTA2*M2)1Jbk | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Jeffrey B Kopp | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | Tg(NPHS2-rtTA)1Jbk; | ||
| Mutation Made By | Jeffrey Kopp, NIDDK, NIH (AMDCC) | ||
| Strain of Origin | FVB/N | ||
| Site of Expression | podocytes within kidney glomeruli | ||
| Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli | ||
| The tetracycline repressor gene (Tetr), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). One mutant with a four amino acid residue change (rTetR) exhibited dependence on tetracycline for induction of the targeted gene and was used in the rtTA construct (Gossen et al, 1995). rTetr was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). | |||
| Promoter | NPHS2, nephrosis 2, idiopathic, steroid-resistant (podocin), human | ||
| Molecular Note | The rtTA gene was placed under the control of the human NPHS2 gene. The resulting transgene is expressed in the kidney when induced by doxycycline. [MGI Ref ID J:109826] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(NPHS2-rtTA2*M2)1Jbk, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Shigehara T; Zaragoza C; Kitiyakara C; Takahashi H; Lu H; Moeller M; Holzman LB; Kopp JB. 2003. Inducible podocyte-specific gene expression in transgenic mice. J Am Soc Nephrol 14(8):1998-2003. [PubMed: 12874453] [MGI Ref ID J:109826]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining the live congenic colony, hemizygous mice are bred with wildtype siblings or to FVB/NJ inbred mice. Mating System Noncarrier x Hemizygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $236.40 Female or Male Hemizygous for Tg(NPHS2-rtTA2*M2)1Jbk *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $288.65 Hemizygous for Tg(NPHS2-rtTA2*M2)1Jbk x Noncarrier $288.65 Noncarrier x Hemizygous for Tg(NPHS2-rtTA2*M2)1Jbk
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $307.40 Female or Male Hemizygous for Tg(NPHS2-rtTA2*M2)1Jbk *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $375.30 Hemizygous for Tg(NPHS2-rtTA2*M2)1Jbk x Noncarrier $375.30 Noncarrier x Hemizygous for Tg(NPHS2-rtTA2*M2)1Jbk
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Terms of Use
Terms of Use
General Terms and Conditions
Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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