Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Coisogenic; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N10pN1   Donating Investigator Jeffrey Kopp,   NIDDK, NIH (AMDCC)

Description
These mice express an optimized reverse tetracycline-controlled transactivator (rtTA2-M2) protein under the control of the human NPHS2 promoter, which directs expression to podocytes within kidney glomeruli. When mated to a second strain carrying a gene under the regulatory control of a tetracycline-responsive promoter element (TRE or tetO), expression of the gene in podocytes is induced with administration of the tetracycline analog, doxycycline (dox). These podocin-rtTA mice provide a Tet-On tool that allows dox-inducible expression of genes in podocytes, and may be useful in studying the role of podocyte nephrobiology in renal disorders.

Development
The podocin-rtTA (or NPHS2-rtTA) construct was designed with a 2.5 kb fragment of the podocin (or NPHS2) promoter/enhancer region upstream of an optimized reverse tetracycline-controlled transactivator variant (rtTA2-M2; modified with humanized codon usage and the FFF minimal activator domains), followed by an SV40 3' UTR and polyA region. This transgene was microinjected into the pronuclei of fertilized FVB/N oocytes. Founder mice were bred to FVB/N inbred mice. This strain was maintained on the FVB/N genetic background prior to arrival at The Jackson Laboratory. Upon arrival, mice may have been bred to FVB/NJ inbred mice (Stock No. 001800) to establish the colony.

Control Information

	Control
	Noncarrier
	001800 FVB/NJ
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of NPHS2

008205

B6.Cg-Tg(NPHS2-cre)295Lbh/J

View Strains carrying other alleles of NPHS2     (1 strain)

Strains carrying other alleles of rtTA

006965	B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae/J
005670	B6.Cg-Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
007176	B6.Cg-Tg(Pax8-rtTA2S*M2)1Koes/J
006235	B6.Cg-Tg(SFTPC-rtTA)5Jaw/J
006232	B6.Cg-Tg(Scgb1a1-rtTA)1Jaw/J
006911	B6;129-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm2(tetO-Pou5f1)Jae/J
007678	B6;SJL-Tg(KRT14-rtTA)208Jek/J
010576	B6;SJL-Tg(MMTV-rtTA)4-1Jek/J
006245	C.Cg-Tg(SFTPC-rtTA)5Jaw/J
006242	C.Cg-Tg(Scgb1a1-rtTA)1Jaw/J
008099	FVB-Tg(KRT14-rtTA)F42Efu/J
004127	FVB-Tg(Nes-rtTA)306Rvs/J
006225	FVB.Cg-Tg(SFTPC-rtTA)5Jaw/J
006222	FVB.Cg-Tg(Scgb1a1-rtTA)1Jaw/J
006875	FVB/N-Tg(Tagln-rtTA)E1Jwst/J
004602	NOD.Cg-Tg(Ins2-rtTA)2Doi/DoiJ
005734	NOD/Lt-Tg(Ins2-rtTA)1Ach/AchJ
005572	STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
008755	STOCK Tg(Ins2-rtTA)2Efr Tg(teto-DTA)1Gfi/J
008250	STOCK Tg(Ins2-rtTA)2Efr/J
005493	STOCK Tg(Tek-rtTA,TRE-lacZ)1425Tpr/J
003273	STOCK Tg(rtTAhCMV)4Bjd/J

View Strains carrying other alleles of rtTA     (22 strains)

Additional Web Information

Tet Expression Systems

Phenotype

Phenotype Information

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Research Tools
Genetics Research
      Mutagenesis and Transgenesis: Tetop Tet System
      Tissue/Cell Markers: kidney specific marker
Tet Expression Systems
      tTA/rtTA Expressing Strains

Genes & Alleles

Gene & Allele Information

Allele Symbol		Tg(NPHS2-rtTA2*M2)1Jbk
Allele Name		transgene insertion 1, Jeffrey B Kopp
Allele Type		Transgenic (random, expressed)
Common Name(s)		Tg(NPHS2-rtTA)1Jbk; podocin-rtTA;
Mutation Made By		Jeffrey Kopp,   NIDDK, NIH (AMDCC)
Strain of Origin		FVB/N
Site of Expression		podocytes within kidney glomeruli
Expressed Gene		rtTA, reverse tetracycline-controlled transactivator, E. coli
		The tetracycline repressor gene (Tetr), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). One mutant with a four amino acid residue change (rTetR) exhibited dependence on tetracycline for induction of the targeted gene and was used in the rtTA construct (Gossen et al, 1995). rTetr was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16).
Promoter		NPHS2, nephrosis 2, idiopathic, steroid-resistant (podocin), human
Molecular Note		The rtTA gene was placed under the control of the human NPHS2 gene. The resulting transgene is expressed in the kidney when induced by doxycycline. [MGI Ref ID J:109826]

Genotyping

Genotyping Information

Genotyping Protocols

Tg(NPHS2-rtTA2*M2)1Jbk, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Shigehara T; Zaragoza C; Kitiyakara C; Takahashi H; Lu H; Moeller M; Holzman LB; Kopp JB. 2003. Inducible podocyte-specific gene expression in transgenic mice. J Am Soc Nephrol 14(8):1998-2003. [PubMed: 12874453]  [MGI Ref ID J:109826]

Additional References

Tg(NPHS2-rtTA2*M2)1Jbk related

Davis B; Dei Cas A; Long DA; White KE; Hayward A; Ku CH; Woolf AS; Bilous R; Viberti G; Gnudi L. 2007. Podocyte-specific expression of angiopoietin-2 causes proteinuria and apoptosis of glomerular endothelia. J Am Soc Nephrol 18(8):2320-9. [PubMed: 17625119]  [MGI Ref ID J:148150]

Ku CH; White KE; Dei Cas A; Hayward A; Webster Z; Bilous R; Marshall S; Viberti G; Gnudi L. 2008. Inducible overexpression of sFlt-1 in podocytes ameliorates glomerulopathy in diabetic mice. Diabetes 57(10):2824-33. [PubMed: 18647955]  [MGI Ref ID J:141994]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	When maintaining the live congenic colony, hemizygous mice are bred with wildtype siblings or to FVB/NJ inbred mice.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location). This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Noncarrier
	001800 FVB/NJ
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
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Terms of Use

Terms of Use

General Terms and Conditions

Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.

For additional Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.

Contact information

General inquiries

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phone:	207-288-6470
fax:	207-288-6655

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