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| These podocin-rtTA mice provide a Tet-On tool that allows dox-inducible expression of genes in podocytes, and may be useful in studying the role of podocyte nephrobiology in renal disorders. | |||||||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Coisogenic; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N10pN1
Generation DefinitionsDonating Investigator Jeffrey Kopp, NIDDK, NIH (AMDCC) Description
These mice express an optimized reverse tetracycline-controlled transactivator (rtTA2-M2) protein under the control of the human NPHS2 promoter, which directs expression to podocytes within kidney glomeruli. When mated to a second strain carrying a gene under the regulatory control of a tetracycline-responsive promoter element (TRE or tetO), expression of the gene in podocytes is induced with administration of the tetracycline analog, doxycycline (dox). These podocin-rtTA mice provide a Tet-On tool that allows dox-inducible expression of genes in podocytes, and may be useful in studying the role of podocyte nephrobiology in renal disorders.Development
The podocin-rtTA (or NPHS2-rtTA) construct was designed with a 2.5 kb fragment of the podocin (or NPHS2) promoter/enhancer region upstream of an optimized reverse tetracycline-controlled transactivator variant (rtTA2-M2; modified with humanized codon usage and the FFF minimal activator domains), followed by an SV40 3' UTR and polyA region. This transgene was microinjected into the pronuclei of fertilized FVB/N oocytes. Founder mice were bred to FVB/N inbred mice. This strain was maintained on the FVB/N genetic background prior to arrival at The Jackson Laboratory. Upon arrival, mice may have been bred to FVB/NJ inbred mice (Stock No. 001800) to establish the colony.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of NPHS2
008523 129S6.Cg-Tg(NPHS2-cre)295Lbh/BroJ 008205 B6.Cg-Tg(NPHS2-cre)295Lbh/J View Strains carrying other alleles of NPHS2 (2 strains)
Additional Web Information
Tet Expression Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Research Tools
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Tetop Tet System
Tissue/Cell Markers
Tissue/Cell Markers: kidney specific marker
Tet Expression Systems
tTA/rtTA Expressing Strains
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(NPHS2-rtTA2*M2)1Jbk | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Jeffrey B Kopp | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | Tg(NPHS2-rtTA)1Jbk; podocin-rtTA; | ||
| Mutation Made By | Jeffrey Kopp, NIDDK, NIH (AMDCC) | ||
| Strain of Origin | FVB/N | ||
| Site of Expression | podocytes within kidney glomeruli | ||
| Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli | ||
| The tetracycline repressor gene (Tetr), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). One mutant with a four amino acid residue change (rTetR) exhibited dependence on tetracycline for induction of the targeted gene and was used in the rtTA construct (Gossen et al, 1995). rTetr was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). | |||
| Promoter | NPHS2, nephrosis 2, idiopathic, steroid-resistant (podocin), human | ||
| Molecular Note | The rtTA gene was placed under the control of the human NPHS2 gene. The resulting transgene is expressed in the kidney when induced by doxycycline. [MGI Ref ID J:109826] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(NPHS2-rtTA2*M2)1Jbk, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Shigehara T; Zaragoza C; Kitiyakara C; Takahashi H; Lu H; Moeller M; Holzman LB; Kopp JB. 2003. Inducible podocyte-specific gene expression in transgenic mice. J Am Soc Nephrol 14(8):1998-2003. [PubMed: 12874453] [MGI Ref ID J:109826]
Additional References
Tg(NPHS2-rtTA2*M2)1Jbk relatedBollee G; Flamant M; Schordan S; Fligny C; Rumpel E; Milon M; Schordan E; Sabaa N; Vandermeersch S; Galaup A; Rodenas A; Casal I; Sunnarborg SW; Salant DJ; Kopp JB; Threadgill DW; Quaggin SE; Dussaule JC; Germain S; Mesnard L; Endlich K; Boucheix C; Belenfant X; Callard P; Endlich N; Tharaux PL. 2011. Epidermal growth factor receptor promotes glomerular injury and renal failure in rapidly progressive crescentic glomerulonephritis. Nat Med 17(10):1242-50. [PubMed: 21946538] [MGI Ref ID J:177277]
Davis B; Dei Cas A; Long DA; White KE; Hayward A; Ku CH; Woolf AS; Bilous R; Viberti G; Gnudi L. 2007. Podocyte-specific expression of angiopoietin-2 causes proteinuria and apoptosis of glomerular endothelia. J Am Soc Nephrol 18(8):2320-9. [PubMed: 17625119] [MGI Ref ID J:148150]
Eremina V; Jefferson JA; Kowalewska J; Hochster H; Haas M; Weisstuch J; Richardson C; Kopp JB; Kabir MG; Backx PH; Gerber HP; Ferrara N; Barisoni L; Alpers CE; Quaggin SE. 2008. VEGF inhibition and renal thrombotic microangiopathy. N Engl J Med 358(11):1129-36. [PubMed: 18337603] [MGI Ref ID J:165015]
Godel M; Hartleben B; Herbach N; Liu S; Zschiedrich S; Lu S; Debreczeni-Mor A; Lindenmeyer MT; Rastaldi MP; Hartleben G; Wiech T; Fornoni A; Nelson RG; Kretzler M; Wanke R; Pavenstadt H; Kerjaschki D; Cohen CD; Hall MN; Ruegg MA; Inoki K; Walz G; Huber TB. 2011. Role of mTOR in podocyte function and diabetic nephropathy in humans and mice. J Clin Invest 121(6):2197-209. [PubMed: 21606591] [MGI Ref ID J:174021]
Jones N; New LA; Fortino MA; Eremina V; Ruston J; Blasutig IM; Aoudjit L; Zou Y; Liu X; Yu GL; Takano T; Quaggin SE; Pawson T. 2009. Nck proteins maintain the adult glomerular filtration barrier. J Am Soc Nephrol 20(7):1533-43. [PubMed: 19443634] [MGI Ref ID J:164965]
Kato H; Gruenwald A; Suh JH; Miner JH; Barisoni-Thomas L; Taketo MM; Faul C; Millar SE; Holzman LB; Susztak K. 2011. Wnt/beta-catenin pathway in podocytes integrates cell adhesion, differentiation, and survival. J Biol Chem 286(29):26003-15. [PubMed: 21613219] [MGI Ref ID J:175392]
Ku CH; White KE; Dei Cas A; Hayward A; Webster Z; Bilous R; Marshall S; Viberti G; Gnudi L. 2008. Inducible overexpression of sFlt-1 in podocytes ameliorates glomerulopathy in diabetic mice. Diabetes 57(10):2824-33. [PubMed: 18647955] [MGI Ref ID J:141994]
Nijenhuis T; Sloan AJ; Hoenderop JG; Flesche J; van Goor H; Kistler AD; Bakker M; Bindels RJ; de Boer RA; Moller CC; Hamming I; Navis G; Wetzels JF; Berden JH; Reiser J; Faul C; van der Vlag J. 2011. Angiotensin II contributes to podocyte injury by increasing TRPC6 expression via an NFAT-mediated positive feedback signaling pathway. Am J Pathol 179(4):1719-32. [PubMed: 21839714] [MGI Ref ID J:179938]
Reidy KJ; Villegas G; Teichman J; Veron D; Shen W; Jimenez J; Thomas D; Tufro A. 2009. Semaphorin3a regulates endothelial cell number and podocyte differentiation during glomerular development. Development 136(23):3979-89. [PubMed: 19906865] [MGI Ref ID J:154978]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.Colony Maintenance
Breeding & Husbandry When maintaining the live congenic colony, hemizygous mice are bred with wildtype siblings or to FVB/NJ inbred mice.
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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General Terms and Conditions
Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
For additional Licensing and Use Restrictions view the link(s) below:
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