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| These Oct4-neo mutant mice may be useful for the selection of induced pluripotent stem (iPS) cells. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System +/+ sibling x Heterozygote (Female x Male) 13-JAN-09 Species laboratory mouse Generation F?+3 (18-DEC-08) Donating Investigator Rudolf Jaenisch, Whitehead Institute (MIT) Description
Mice heterozygous for this Oct4-neo mutation are viable and fertile, harboring an IRES-GFPneo fusion cassette downstream of exon 5 of the Oct4 (Pou5f1) gene. The donating investigator reports (and attempts at The Jackson Laboratory confirm) no living homozygous animals are born. While sufficient neomycin-resistance activity is observed in embryonic stem cells, no visible GFP fluorescence is reported. When treated with specific transcription factors (Oct4, Sox2, c-Myc and Klf4), some Oct4-neo murine embryonic fibroblasts (MEFs) have the properties of induced pluripotent stem (iPS) cells. Such iPS cells have the DNA methylation, gene expression and chromatin state of embryonic stem cells and can form viable chimeras, contribute to the germ line, and generate live late-term embryos when injected into tetraploid blastocysts. These Oct4-neo mutant mice may be useful for the selection of iPS cells (i.e. epigenetic reprogramming of somatic cells into pluripotent embryonic stem cells).Of note, these Oct4-neo mutant mice may also be used in conjunction with Oct4-EGFP mice (Stock No. 008214); a similar mutant strain bearing an EGFP-expressing mutation from the Oct4 locus.
Development
The Oct4-IRES-GFPneo targeting vector was designed to insert an IRES-GFPneo fusion cassette downstream of exon 5 of the Oct4 (Pou5f1) gene. This construct was microinjected into (C57BL/6 x 129S4Sv/Jae)F1-derived V6.5 embryonic stem (ES) cells. Correctly targeted ES cells (with sufficient neomycin-resistance activity but no visible GFP fluorescence) were injected into blastocysts. Chimeric mice were bred to C57BL/6NTac mice to establish the mutant colony. The resulting Oct4-neo mutant mice were maintained on a mixed B6;129S4 genetic background prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| Wild-type siblings from the colony | ||
| 101043 B6129SF1/J | (approximate) | |
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Pou5f1
006911 B6;129-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm2(tetO-Pou5f1)Jae/J 008214 B6;129S4-Pou5f1tm2Jae/J 004654 B6;CBA-Tg(Pou5f1-EGFP)2Mnn/J View Strains carrying other alleles of Pou5f1 (3 strains)
Phenotype
Phenotype Information
assigned by genotype
Pou5f1tm1Jae/Pou5f1tm1Jae
involves: 129S4/SvJae * C57BL/6
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:130250)
- mice are viable and fertile; some murine embryonic fibroblasts treated with specific transcription factors (Pou5f1, Sox2, c-myc, and Klf4) have the properties of induced pluripotent stem (iPS) cells. Such iPS cells have the DNA methylation, gene expression and chromatin state of embryonic stem cells and can form viable chimeras, contribute to the germ line, and generate live late-term embryos when injected into tetraploid blastocysts.
This mouse can be used to support research in many areas including:
Developmental Biology Research
Embryonic Lethality (Homozygous)
Research Tools
Cell Biology Research
Developmental Biology Research
General Purpose
Genetics Research
Mutagenesis and Transgenesis: Production of Targeted Mutations ("Knockouts")
Mutagenesis and Transgenesis: Production of Transgenic Mice
Mutagenesis and Transgenesis: Production of Transgenics
Tissue/Cell Markers
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Pou5f1tm1Jae | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Rudolf Jaenisch | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | Oct4-IRES-GFPneo; Oct4-neo; | ||
| Mutation Made By | Rudolf Jaenisch, Whitehead Institute (MIT) | ||
| Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 | ||
| ES Cell Line Name | v6.5 | ||
| ES Cell Line Strain | (C57BL/6 x 129S4/SvJae)F1 | ||
| Gene Symbol and Name | Pou5f1, POU domain, class 5, transcription factor 1 | ||
| Chromosome | 17 | ||
| Gene Common Name(s) | MGC22487; OCT3; OCT4; OTF3; OTF4; Oct-3; Oct-3/4; Oct-4; Oct3/4; Otf-3; Otf-4; Otf3; Otf3-rs7; Otf3g; Otf4; octamer binding transcription factor 3; octamer binding transcription factor 3 related sequence 7; octamer binding transcription factor 4; | ||
| General Note | When treated with specific transcription factors (Oct4, Sox2, c-Myc and Klf4), some Oct4-neo murine embryonic fibroblasts (MEFs) have the properties of induced pluripotent stem (iPS) cells. Such iPS cells have the DNA methylation, gene expression and chromatin state of embryonic stem cells and can form viable chimeras, contribute to the germ line, and generate live late-term embryos when injected into tetraploid blastocysts. | ||
| Molecular Note | The Oct4-IRES-GFPneo targeting vector was designed to insert an IRES-GFPneo fusion cassette downstream of exon 5. While sufficient neomycin-resistance activity is observed in embryonic stem cells, no visible GFP fluorescence is reported. [MGI Ref ID J:130250] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Pou5f1tm1Jae, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Wernig M; Meissner A; Foreman R; Brambrink T; Ku M; Hochedlinger K; Bernstein BE; Jaenisch R. 2007. In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state. Nature 448(7151):318-24. [PubMed: 17554336] [MGI Ref ID J:130250]
Additional References
Pou5f1tm1Jae relatedHanna J; Wernig M; Markoulaki S; Sun CW; Meissner A; Cassady JP; Beard C; Brambrink T; Wu LC; Townes TM; Jaenisch R. 2007. Treatment of sickle cell anemia mouse model with iPS cells generated from autologous skin. Science 318(5858):1920-3. [PubMed: 18063756] [MGI Ref ID J:130467]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, heterozygous mice may be bred together or to wildtype siblings. Mating System +/+ sibling x Heterozygote (Female x Male) 13-JAN-09 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Heterozygous for Pou5f1tm1Jae
Pairs /Price (US dollars $) Pair Genotype $297.85 Heterozygous for Pou5f1tm1Jae x Wild-type for Pou5f1tm1Jae $297.85 Wild-type for Pou5f1tm1Jae x Heterozygous for Pou5f1tm1Jae
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Heterozygous for Pou5f1tm1Jae
Pairs /Price (US dollars $) Pair Genotype $387.30 Heterozygous for Pou5f1tm1Jae x Wild-type for Pou5f1tm1Jae $387.30 Wild-type for Pou5f1tm1Jae x Heterozygous for Pou5f1tm1Jae
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Wild-type siblings from the colony | ||
| 101043 B6129SF1/J | (approximate) | |
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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