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| These Podocin-Cre mice (mice harboring the p2.5P-Cre transgene) have expression of Cre recombinase directed to podocytes within kidney glomeruli by the human podocin (NPHS2) promoter/enhancer region and may be useful in generating Cre-lox conditional knockouts for studying the role of podocyte nephrobiology in renal disorders. | |||||||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
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Description
Strain Information
Type Congenic; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Noncarrier x Hemizygote (Female x Male) 26-AUG-09 Species laboratory mouse Generation N11+N (07-OCT-09)
Generation DefinitionsDonating Investigator Lawrence Holzman, University of Michigan Description
Podocin-Cre mice (mice harboring the p2.5P-Cre transgene) are viable and fertile, with expression of Cre recombinase directed to podocytes within kidney glomeruli by the human podocin (NPHS2) promoter/enhancer region. Cre-recombinase activity is reported in podocytes during late capillary loop stage of glomerular development and persists in podocytes of mature glomeruli, with no evidence for cre expression detected in other tissues examined. Embryonic Cre-recombinase activity is also reported as early as 8.5 dpc. When bred with mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of the sequence. These Podocin-Cre mice (mice harboring the p2.5P-Cre transgene) may be useful in generating conditional knockouts for studying the role of podocyte nephrobiology in renal disorders.Development
The p2.5P-Cre transgene was designed with a 2.5 kb fragment of the human podocin (or NPHS2) promoter/enhancer region upstream of a Cre recombinase cassette followed by a murine protamine poly-adenylation signal. The donating investigator reports that the transgene was microinjected into (C57BL/6 X SJL)F2 mouse eggs. Mice from founder line 295 were found to have podocyte-specific cre activity, were subsequently backcrossed to C57BL/6J for at least ten generations, and then bred together to generate "homozygotes" prior to arrival at The Jackson Laboratory Repository. Upon arrival, the 2.5P-Cre transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Tg(NPHS2-cre)295Lbh allele
008523 129S6.Cg-Tg(NPHS2-cre)295Lbh/BroJ View Strains carrying Tg(NPHS2-cre)295Lbh (1 strain)
008523 129S6.Cg-Tg(NPHS2-cre)295Lbh/BroJ 008202 FVB/N-Tg(NPHS2-rtTA2*M2)1Jbk/J
008523 129S6.Cg-Tg(NPHS2-cre)295Lbh/BroJ
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cardiovascular Research
Cre-lox System
Cre-lox System
Cre Recombinase Expression
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Tissue/Cell Markers: kidney specific marker
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(NPHS2-cre)295Lbh | ||
|---|---|---|---|
| Allele Name | transgene insertion 295, Lawrence B Holzman | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | 2.5P-Cre; NPHS2.Cre; Tg(NPHS2-cre)1Lbh; pod-Cre; podocin-Cre; | ||
| Mutation Made By | Lawrence Holzman, University of Michigan | ||
| Strain of Origin | (C57BL/6 x SJL)F2 | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | NPHS2, nephrosis 2, idiopathic, steroid-resistant (podocin), human | ||
| Driver Note | NPHS2 | ||
| General Note | 24 transgenic founders were created. Phenotypic analysis was performed on founder line #295. | ||
| Molecular Note | The human NPHS2 promoter, including the entire 5' untranslated region, was used to drive cre recombinase expression in podocytes. [MGI Ref ID J:81633] | ||
| Gene Symbol and Name | Tg(NPHS2-cre)295Lbh, transgene insertion 295, Lawrence B Holzman | ||
| Chromosome | UN | ||
| Gene Common Name(s) | 2.5P-Cre; Tg(NPHS2-cre)1Lbh; pod-Cre; podocin-Cre; transgene insertion 1, Lawrence B Holzman; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Moeller MJ; Sanden SK; Soofi A; Wiggins RC; Holzman LB. 2003. Podocyte-specific expression of cre recombinase in transgenic mice. Genesis 35(1):39-42. [PubMed: 12481297] [MGI Ref ID J:81633]
Additional References
Tg(NPHS2-cre)295Lbh relatedArora S; Husain M; Kumar D; Patni H; Pathak S; Mehrotra D; Reddy VK; Reddy LR; Salhan D; Yadav A; Mathieson PW; Saleem MA; Chander PN; Singhal PC. 2009. Human immunodeficiency virus downregulates podocyte apoE expression. Am J Physiol Renal Physiol 297(3):F653-61. [PubMed: 19553347] [MGI Ref ID J:152103]
Bollee G; Flamant M; Schordan S; Fligny C; Rumpel E; Milon M; Schordan E; Sabaa N; Vandermeersch S; Galaup A; Rodenas A; Casal I; Sunnarborg SW; Salant DJ; Kopp JB; Threadgill DW; Quaggin SE; Dussaule JC; Germain S; Mesnard L; Endlich K; Boucheix C; Belenfant X; Callard P; Endlich N; Tharaux PL. 2011. Epidermal growth factor receptor promotes glomerular injury and renal failure in rapidly progressive crescentic glomerulonephritis. Nat Med 17(10):1242-50. [PubMed: 21946538] [MGI Ref ID J:177277]
Brukamp K; Jim B; Moeller MJ; Haase VH. 2007. Hypoxia and podocyte-specific Vhlh deletion confer risk of glomerular disease. Am J Physiol Renal Physiol 293(4):F1397-407. [PubMed: 17609290] [MGI Ref ID J:143007]
Chen S; Wassenhove-McCarthy DJ; Yamaguchi Y; Holzman LB; van Kuppevelt TH; Jenniskens GJ; Wijnhoven TJ; Woods AC; McCarthy KJ. 2008. Loss of heparan sulfate glycosaminoglycan assembly in podocytes does not lead to proteinuria. Kidney Int 74(3):289-99. [PubMed: 18480751] [MGI Ref ID J:152871]
Dai C; Stolz DB; Bastacky SI; St-Arnaud R; Wu C; Dedhar S; Liu Y. 2006. Essential role of integrin-linked kinase in podocyte biology: Bridging the integrin and slit diaphragm signaling. J Am Soc Nephrol 17(8):2164-75. [PubMed: 16837631] [MGI Ref ID J:129286]
Dai C; Stolz DB; Kiss LP; Monga SP; Holzman LB; Liu Y. 2009. Wnt/beta-catenin signaling promotes podocyte dysfunction and albuminuria. J Am Soc Nephrol 20(9):1997-2008. [PubMed: 19628668] [MGI Ref ID J:166330]
Garg P; Verma R; Cook L; Soofi A; Venkatareddy M; George B; Mizuno K; Gurniak C; Witke W; Holzman LB. 2010. Actin-depolymerizing factor cofilin-1 is necessary in maintaining mature podocyte architecture. J Biol Chem 285(29):22676-88. [PubMed: 20472933] [MGI Ref ID J:165315]
Godel M; Hartleben B; Herbach N; Liu S; Zschiedrich S; Lu S; Debreczeni-Mor A; Lindenmeyer MT; Rastaldi MP; Hartleben G; Wiech T; Fornoni A; Nelson RG; Kretzler M; Wanke R; Pavenstadt H; Kerjaschki D; Cohen CD; Hall MN; Ruegg MA; Inoki K; Walz G; Huber TB. 2011. Role of mTOR in podocyte function and diabetic nephropathy in humans and mice. J Clin Invest 121(6):2197-209. [PubMed: 21606591] [MGI Ref ID J:174021]
Hartleben B; Godel M; Meyer-Schwesinger C; Liu S; Ulrich T; Kobler S; Wiech T; Grahammer F; Arnold SJ; Lindenmeyer MT; Cohen CD; Pavenstadt H; Kerjaschki D; Mizushima N; Shaw AS; Walz G; Huber TB. 2010. Autophagy influences glomerular disease susceptibility and maintains podocyte homeostasis in aging mice. J Clin Invest 120(4):1084-96. [PubMed: 20200449] [MGI Ref ID J:159695]
Harvey SJ; Jarad G; Cunningham J; Rops AL; van der Vlag J; Berden JH; Moeller MJ; Holzman LB; Burgess RW; Miner JH. 2007. Disruption of glomerular basement membrane charge through podocyte-specific mutation of agrin does not alter glomerular permselectivity. Am J Pathol 171(1):139-52. [PubMed: 17591961] [MGI Ref ID J:122821]
Huber TB; Hartleben B; Winkelmann K; Schneider L; Becker JU; Leitges M; Walz G; Haller H; Schiffer M. 2009. Loss of podocyte aPKClambda/iota causes polarity defects and nephrotic syndrome. J Am Soc Nephrol 20(4):798-806. [PubMed: 19279126] [MGI Ref ID J:164628]
Jia Q; McDill BW; Sankarapandian B; Wu S; Liapis H; Holzman LB; Capecchi MR; Miner JH; Chen F. 2008. Ablation of developing podocytes disrupts cellular interactions and nephrogenesis both inside and outside the glomerulus. Am J Physiol Renal Physiol 295(6):F1790-8. [PubMed: 18842818] [MGI Ref ID J:166011]
Johnstone DB; Zhang J; George B; Leon C; Gachet C; Wong H; Parekh R; Holzman LB. 2011. Podocyte-specific deletion of Myh9 encoding nonmuscle myosin heavy chain 2A predisposes mice to glomerulopathy. Mol Cell Biol 31(10):2162-70. [PubMed: 21402784] [MGI Ref ID J:173971]
Kato H; Gruenwald A; Suh JH; Miner JH; Barisoni-Thomas L; Taketo MM; Faul C; Millar SE; Holzman LB; Susztak K. 2011. Wnt/beta-catenin pathway in podocytes integrates cell adhesion, differentiation, and survival. J Biol Chem 286(29):26003-15. [PubMed: 21613219] [MGI Ref ID J:175392]
Lefevre GM; Patel SR; Kim D; Tessarollo L; Dressler GR. 2010. Altering a histone H3K4 methylation pathway in glomerular podocytes promotes a chronic disease phenotype. PLoS Genet 6(10):e1001142. [PubMed: 21060806] [MGI Ref ID J:167530]
Moeller MJ; Soofi A; Sanden S; Floege J; Kriz W; Holzman LB. 2005. An efficient system for tissue-specific overexpression of transgenes in podocytes in vivo. Am J Physiol Renal Physiol 289(2):F481-8. [PubMed: 15784842] [MGI Ref ID J:134739]
Peng M; Falk MJ; Haase VH; King R; Polyak E; Selak M; Yudkoff M; Hancock WW; Meade R; Saiki R; Lunceford AL; Clarke CF; L Gasser D. 2008. Primary coenzyme Q deficiency in Pdss2 mutant mice causes isolated renal disease. PLoS Genet 4(4):e1000061. [PubMed: 18437205] [MGI Ref ID J:136670]
Pozzi A; Jarad G; Moeckel GW; Coffa S; Zhang X; Gewin L; Eremina V; Hudson BG; Borza DB; Harris RC; Holzman LB; Phillips CL; Fassler R; Quaggin SE; Miner JH; Zent R. 2008. Beta1 integrin expression by podocytes is required to maintain glomerular structural integrity. Dev Biol 316(2):288-301. [PubMed: 18328474] [MGI Ref ID J:135414]
Sachs N; Kreft M; van den Bergh Weerman MA; Beynon AJ; Peters TA; Weening JJ; Sonnenberg A. 2006. Kidney failure in mice lacking the tetraspanin CD151. J Cell Biol 175(1):33-9. [PubMed: 17015618] [MGI Ref ID J:114988]
Steenhard BM; Isom K; Stroganova L; St John PL; Zelenchuk A; Freeburg PB; Holzman LB; Abrahamson DR. 2010. Deletion of von Hippel-Lindau in glomerular podocytes results in glomerular basement membrane thickening, ectopic subepithelial deposition of collagen {alpha}1{alpha}2{alpha}1(IV), expression of neuroglobin, and proteinuria. Am J Pathol 177(1):84-96. [PubMed: 20522651] [MGI Ref ID J:162099]
Suleiman H; Heudobler D; Raschta AS; Zhao Y; Zhao Q; Hertting I; Vitzthum H; Moeller MJ; Holzman LB; Rachel R; Johnson R; Westphal H; Rascle A; Witzgall R. 2007. The podocyte-specific inactivation of Lmx1b, Ldb1 and E2a yields new insight into a transcriptional network in podocytes. Dev Biol 304(2):701-12. [PubMed: 17316599] [MGI Ref ID J:122505]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice may be bred with wildtype siblings or with C57BL/6J inbred mice. The donating investigator reports that homozygous mice may be bred together. Mating System Noncarrier x Hemizygote (Female x Male) 26-AUG-09 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $261.00 Female or Male Hemizygous for Tg(NPHS2-cre)295Lbh
Pairs /Price (US dollars $) Pair Genotype $321.00 Hemizygous for Tg(NPHS2-cre)295Lbh x Noncarrier $321.00 Noncarrier x Hemizygous for Tg(NPHS2-cre)295Lbh Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $339.30 Female or Male Hemizygous for Tg(NPHS2-cre)295Lbh
Pairs /Price (US dollars $) Pair Genotype $417.30 Hemizygous for Tg(NPHS2-cre)295Lbh x Noncarrier $417.30 Noncarrier x Hemizygous for Tg(NPHS2-cre)295Lbh Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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