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| These Podocin-Cre mice (mice harboring the p2.5P-Cre transgene) have expression of Cre recombinase directed to podocytes within kidney glomeruli by the human podocin (NPHS2) promoter/enhancer region and may be useful in generating Cre-lox conditional knockouts for studying the role of podocyte nephrobiology in renal disorders. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
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Description
Strain Information
Type Congenic; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Noncarrier x Hemizygote (Female x Male) 26-AUG-09 Species laboratory mouse Generation N11+N (07-OCT-09) Donating Investigator Lawrence Holzman, University of Michigan Description
Podocin-Cre mice (mice harboring the p2.5P-Cre transgene) are viable and fertile, with expression of Cre recombinase directed to podocytes within kidney glomeruli by the human podocin (NPHS2) promoter/enhancer region. Cre-recombinase activity is reported in podocytes during late capillary loop stage of glomerular development and persists in podocytes of mature glomeruli, with no evidence for cre expression detected in other tissues examined. Embryonic Cre-recombinase activity is also reported as early as 8.5 dpc. When bred with mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of the sequence. These Podocin-Cre mice (mice harboring the p2.5P-Cre transgene) may be useful in generating conditional knockouts for studying the role of podocyte nephrobiology in renal disorders.Development
The p2.5P-Cre transgene was designed with a 2.5 kb fragment of the human podocin (or NPHS2) promoter/enhancer region upstream of a Cre recombinase cassette followed by a murine protamine poly-adenylation signal. The donating investigator reports that the transgene was microinjected into (C57BL/6 X SJL)F2 mouse eggs. Mice from founder line 295 were found to have podocyte-specific cre activity, were subsequently backcrossed to C57BL/6J for at least 10 generations, and then bred together to generate "homozygotes" prior to arrival at The Jackson Laboratory. Upon arrival, the 2.5P-Cre transgenic mice may have been bred to C57BL/6J inbred mice (Stock No. 000664) to establish the colony.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of NPHS2
008202 FVB/N-Tg(NPHS2-rtTA2*M2)1Jbk/J View Strains carrying other alleles of NPHS2 (1 strain)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cardiovascular Research
Cre-lox System
Cre-lox System
Cre Recombinase Expression
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers: Cre-lox System
Tissue/Cell Markers: kidney specific marker
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(NPHS2-cre)295Lbh | ||
|---|---|---|---|
| Allele Name | transgene insertion 295, Lawrence B Holzman | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | 2.5P-Cre; Tg(NPHS2-cre)1Lbh; pod-Cre; podocin-Cre; | ||
| Mutation Made By | Lawrence Holzman, University of Michigan | ||
| Strain of Origin | (C57BL/6 x SJL)F2 | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | NPHS2, nephrosis 2, idiopathic, steroid-resistant (podocin), human | ||
| Driver Note | NPHS2 | ||
| General Note | 24 transgenic founders were created. Phenotypic analysis was performed on founder line #295. | ||
| Molecular Note | The human NPHS2 promoter, including the entire 5' untranslated region, was used to drive cre recombinase expression in podocytes. [MGI Ref ID J:81633] | ||
| Gene Symbol and Name | Tg(NPHS2-cre)295Lbh, transgene insertion 295, Lawrence B Holzman | ||
| Chromosome | UN | ||
| Gene Common Name(s) | 2.5P-Cre; Tg(NPHS2-cre)1Lbh; pod-Cre; podocin-Cre; transgene insertion 1, Lawrence B Holzman; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Moeller MJ; Sanden SK; Soofi A; Wiggins RC; Holzman LB. 2003. Podocyte-specific expression of cre recombinase in transgenic mice. Genesis 35(1):39-42. [PubMed: 12481297] [MGI Ref ID J:81633]
Additional References
Tg(NPHS2-cre)295Lbh relatedArora S; Husain M; Kumar D; Patni H; Pathak S; Mehrotra D; Reddy VK; Reddy LR; Salhan D; Yadav A; Mathieson PW; Saleem MA; Chander PN; Singhal PC. 2009. Human immunodeficiency virus downregulates podocyte apoE expression. Am J Physiol Renal Physiol 297(3):F653-61. [PubMed: 19553347] [MGI Ref ID J:152103]
Brukamp K; Jim B; Moeller MJ; Haase VH. 2007. Hypoxia and podocyte-specific Vhlh deletion confer risk of glomerular disease. Am J Physiol Renal Physiol 293(4):F1397-407. [PubMed: 17609290] [MGI Ref ID J:143007]
Chen S; Wassenhove-McCarthy DJ; Yamaguchi Y; Holzman LB; van Kuppevelt TH; Jenniskens GJ; Wijnhoven TJ; Woods AC; McCarthy KJ. 2008. Loss of heparan sulfate glycosaminoglycan assembly in podocytes does not lead to proteinuria. Kidney Int 74(3):289-99. [PubMed: 18480751] [MGI Ref ID J:152871]
Dai C; Stolz DB; Bastacky SI; St-Arnaud R; Wu C; Dedhar S; Liu Y. 2006. Essential role of integrin-linked kinase in podocyte biology: Bridging the integrin and slit diaphragm signaling. J Am Soc Nephrol 17(8):2164-75. [PubMed: 16837631] [MGI Ref ID J:129286]
Harvey SJ; Jarad G; Cunningham J; Rops AL; van der Vlag J; Berden JH; Moeller MJ; Holzman LB; Burgess RW; Miner JH. 2007. Disruption of glomerular basement membrane charge through podocyte-specific mutation of agrin does not alter glomerular permselectivity. Am J Pathol 171(1):139-52. [PubMed: 17591961] [MGI Ref ID J:122821]
Moeller MJ; Soofi A; Sanden S; Floege J; Kriz W; Holzman LB. 2005. An efficient system for tissue-specific overexpression of transgenes in podocytes in vivo. Am J Physiol Renal Physiol 289(2):F481-8. [PubMed: 15784842] [MGI Ref ID J:134739]
Peng M; Falk MJ; Haase VH; King R; Polyak E; Selak M; Yudkoff M; Hancock WW; Meade R; Saiki R; Lunceford AL; Clarke CF; L Gasser D. 2008. Primary coenzyme Q deficiency in Pdss2 mutant mice causes isolated renal disease. PLoS Genet 4(4):e1000061. [PubMed: 18437205] [MGI Ref ID J:136670]
Pozzi A; Jarad G; Moeckel GW; Coffa S; Zhang X; Gewin L; Eremina V; Hudson BG; Borza DB; Harris RC; Holzman LB; Phillips CL; Fassler R; Quaggin SE; Miner JH; Zent R. 2008. Beta1 integrin expression by podocytes is required to maintain glomerular structural integrity. Dev Biol 316(2):288-301. [PubMed: 18328474] [MGI Ref ID J:135414]
Sachs N; Kreft M; van den Bergh Weerman MA; Beynon AJ; Peters TA; Weening JJ; Sonnenberg A. 2006. Kidney failure in mice lacking the tetraspanin CD151. J Cell Biol 175(1):33-9. [PubMed: 17015618] [MGI Ref ID J:114988]
Suleiman H; Heudobler D; Raschta AS; Zhao Y; Zhao Q; Hertting I; Vitzthum H; Moeller MJ; Holzman LB; Rachel R; Johnson R; Westphal H; Rascle A; Witzgall R. 2007. The podocyte-specific inactivation of Lmx1b, Ldb1 and E2a yields new insight into a transcriptional network in podocytes. Dev Biol 304(2):701-12. [PubMed: 17316599] [MGI Ref ID J:122505]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice may be bred with wildtype siblings or with C57BL/6J inbred mice. The donating investigator reports that homozygous mice may be bred together. Mating System Noncarrier x Hemizygote (Female x Male) 26-AUG-09 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(NPHS2-cre)295Lbh
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Tg(NPHS2-cre)295Lbh x Noncarrier $297.85 Noncarrier x Hemizygous for Tg(NPHS2-cre)295Lbh
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(NPHS2-cre)295Lbh
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Tg(NPHS2-cre)295Lbh x Noncarrier $387.30 Noncarrier x Hemizygous for Tg(NPHS2-cre)295Lbh
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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