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| These Stra8-cre transgenic mice have expression of an optimized variant of Cre recombinase (iCre) directed by the 1.4 kb mouse Stra8 (stimulated by retinoic acid gene 8) genomic promoter fragment. When Stra8-cre transgenic males are bred with female mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of the floxed sequence specifically during these stages of spermatogenesis. These Stra8-cre transgenic mice may be useful in generating conditional knockouts in postnatal, premeiotic, male germ cells for studying spermatogenesis. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Noncarrier x Hemizygote (Female x Male) 10-MAR-09 Species laboratory mouse Generation N8+N1F6 (29-JUN-11)
Generation DefinitionsDonating Investigator Robert E Braun, The Jackson Laboratory Description
Homozygous Stra8-cre transgenic mice are viable and fertile, with expression of an optimized variant of Cre recombinase (iCre) directed by the 1.4 kb mouse Stra8 (stimulated by retinoic acid gene 8) genomic promoter fragment. Postnatal Cre-recombinase expression is first observed in testes at postnatal day (P)3 (early-stage spermatogonia), increases out to P7 (pre-leptotene-stage spermatocytes), and is not detected in other tissues examined (including ovaries). In addition, no cre expression is reported in male or female embryos. When Stra8-cre transgenic males are bred with female mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of the floxed sequence, specifically during these stages of spermatogenesis. These Stra8-cre transgenic mice may be useful in generating conditional knockouts in postnatal, premeiotic, male germ cells for studying spermatogenesis.Development
The 3.1 kb Stra8.iCre transgene was designed with the 1.4 kb mouse Stra8 (mouse stimulated by retinoic acid gene 8) genomic promoter fragment (containing the entire promoter region from -1400 to +7), an SV40 splice donor/splice acceptor region, an optimized variant of Cre recombinase (iCre; improved with mammalian codon usage, removed putative cryptic splce sites, altered stop codon, and reduced CpG content to limit the chances of epigenetic silencing in mammals), and an SV40 polyA signal. The transgene was microinjected into the pronucleus of fertilized FVB eggs. The single founder male exhibiting germline expression of the transgene was generated. The donating investigator reports the resulting Stra8-cre mice were subsequently bred to wild-type FVB/NJ mice for many generations prior to arrival at The Jackson Laboratory Repository. Upon arrival, the Stra8-cre transgenic mice were bred to FVB/NJ inbred mice (Stock No. 001800) to establish the colony.A 27 SNP (single nucleotide polymorphism) panel analysis performed by The Jackson Laboratory on the rederived mice revealed nine segregating markers (eight different chromosomes). Four of the loci indicate C57BL/6, while the other five loci may be C57BL/6 or other (July 2009).
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Tg(Stra8-cre)1Reb allele
017490 B6.FVB-Tg(Stra8-cre)1Reb/LguJ View Strains carrying Tg(Stra8-cre)1Reb (1 strain)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(Stra8-cre)1Reb/0
involves: FVB
- normal phenotype
- no abnormal phenotype detected
- mice are viable and fertile; no phenotypic details are provided (MGI Ref ID J:148746)
This mouse can be used to support research in many areas including:
cre relatedReproductive Biology Research
Developmental Defects Affecting Gonads
males only
Fertility Defects
males only
Research Tools
Cre-lox System
Cre Recombinase Expression
Developmental Biology Research
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Tissue/Cell Markers: spermatogonial transplantation marker
Tissue/Cell Markers: testicular cell marker
Reproductive Biology Research
Cre-lox System
male germ cells
spermatogonial transplantation marker
testicular cell marker
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Stra8-cre)1Reb | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Robert E Braun | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | Stra8-cre; Stra8-iCre; Tg(Stra8-iCre)1Reb; | ||
| Mutation Made By | Robert Braun, The Jackson Laboratory | ||
| Strain of Origin | FVB/NJ | ||
| Site of Expression | Postnatal Cre-recombinase expression is first observed in testes at postnatal day (P)3 (early-stage spermatogonia), increases out to P7 (pre-leptotene-stage spermatocytes), and is not detected in other tissues examined (including ovaries). In addition, no cre expression is reported in male or female embryos. | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Stra8, stimulated by retinoic acid gene 8, mouse, laboratory | ||
| Driver Note | Stra8 | ||
| Molecular Note | A 1.4Kb promoter fragment of Stra8 was inserted upstream of the 1.26Kb improved Cre recombinase coding sequence, separated by SV40 splice donor/splice acceptor sequence. The Stra8 promoter region used was -1400 to +7, inserted into the plasmid vector with AvrII and XhoI recognition sites, respectively. [MGI Ref ID J:148746] | ||
| Gene Symbol and Name | Tg(Stra8-cre)1Reb, transgene insertion 1, Robert E Braun | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Stra8-cre; Tg(Stra8-iCre)1Reb; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Stra8-cre), Melt Curve Analysis
Tg(Stra8-cre), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Sadate-Ngatchou PI; Payne CJ; Dearth AT; Braun RE. 2008. Cre recombinase activity specific to postnatal, premeiotic male germ cells in transgenic mice. Genesis 46(12):738-42. [PubMed: 18850594] [MGI Ref ID J:148746]
Additional References
Tg(Stra8-cre)1Reb relatedBatista F; Lu L; Williams SA; Stanley P. 2012. Complex N-glycans are essential, but core 1 and 2 mucin O-glycans, O-fucose glycans, and NOTCH1 are dispensable, for mammalian spermatogenesis. Biol Reprod 86(6):179. [PubMed: 22492969] [MGI Ref ID J:185823]
Gallagher SJ; Kofman AE; Huszar JM; Dannenberg JH; DePinho RA; Braun RE; Payne CJ. 2013. Distinct requirements for Sin3a in perinatal male gonocytes and differentiating spermatogonia. Dev Biol 373(1):83-94. [PubMed: 23085237] [MGI Ref ID J:192193]
Greenlee AR; Shiao MS; Snyder E; Buaas FW; Gu T; Stearns TM; Sharma M; Murchison EP; Puente GC; Braun RE. 2012. Deregulated sex chromosome gene expression with male germ cell-specific loss of Dicer1. PLoS One 7(10):e46359. [PubMed: 23056286] [MGI Ref ID J:192105]
Hobbs RM; Fagoonee S; Papa A; Webster K; Altruda F; Nishinakamura R; Chai L; Pandolfi PP. 2012. Functional antagonism between Sall4 and Plzf defines germline progenitors. Cell Stem Cell 10(3):284-98. [PubMed: 22385656] [MGI Ref ID J:185669]
Huang Z; Rivas B; Agoulnik AI. 2012. Insulin-like 3 signaling is important for testicular descent but dispensable for spermatogenesis and germ cell survival in adult mice. Biol Reprod 87(6):143. [PubMed: 23100620] [MGI Ref ID J:194006]
Liu D; Li L; Fu H; Li S; Li J. 2012. Inactivation of Dicer1 has a severe cumulative impact on the formation of mature germ cells in mouse testes. Biochem Biophys Res Commun 422(1):114-20. [PubMed: 22564735] [MGI Ref ID J:184517]
Lyndaker AM; Lim PX; Mleczko JM; Diggins CE; Holloway JK; Holmes RJ; Kan R; Schlafer DH; Freire R; Cohen PE; Weiss RS. 2013. Conditional inactivation of the DNA damage response gene Hus1 in mouse testis reveals separable roles for components of the RAD9-RAD1-HUS1 complex in meiotic chromosome maintenance. PLoS Genet 9(2):e1003320. [PubMed: 23468651] [MGI Ref ID J:195191]
Navarro VM; Gottsch ML; Wu M; Garcia-Galiano D; Hobbs SJ; Bosch MA; Pinilla L; Clifton DK; Dearth A; Ronnekleiv OK; Braun RE; Palmiter RD; Tena-Sempere M; Alreja M; Steiner RA. 2011. Regulation of NKB pathways and their roles in the control of Kiss1 neurons in the arcuate nucleus of the male mouse. Endocrinology 152(11):4265-75. [PubMed: 21914775] [MGI Ref ID J:178520]
Premsrirut PK; Dow LE; Kim SY; Camiolo M; Malone CD; Miething C; Scuoppo C; Zuber J; Dickins RA; Kogan SC; Shroyer KR; Sordella R; Hannon GJ; Lowe SW. 2011. A rapid and scalable system for studying gene function in mice using conditional RNA interference. Cell 145(1):145-58. [PubMed: 21458673] [MGI Ref ID J:171191]
Song R; Hennig GW; Wu Q; Jose C; Zheng H; Yan W. 2011. Male germ cells express abundant endogenous siRNAs. Proc Natl Acad Sci U S A 108(32):13159-64. [PubMed: 21788498] [MGI Ref ID J:175627]
Tong MH; Yang QE; Davis JC; Griswold MD. 2013. Retinol dehydrogenase 10 is indispensible for spermatogenesis in juvenile males. Proc Natl Acad Sci U S A 110(2):543-8. [PubMed: 23267101] [MGI Ref ID J:193281]
Wu Q; Song R; Ortogero N; Zheng H; Evanoff R; Small CL; Griswold MD; Namekawa SH; Royo H; Turner JM; Yan W. 2012. The RNase III enzyme DROSHA is essential for microRNA production and spermatogenesis. J Biol Chem 287(30):25173-90. [PubMed: 22665486] [MGI Ref ID J:188848]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, the donating investigator reports that homozygous mice may be bred together. As Stra8-cre transgene expression is specific to developing sperm, transmission of the transgene must be through the male germline for experiments involving cre-induced recombination. Mating System Noncarrier x Hemizygote (Female x Male) 10-MAR-09 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Hemizygous for Tg(Stra8-cre)1Reb
Price per Pair (US dollars $) Pair Genotype $296.00 Hemizygous for Tg(Stra8-cre)1Reb x Noncarrier $296.00 Noncarrier x Hemizygous for Tg(Stra8-cre)1Reb Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Hemizygous for Tg(Stra8-cre)1Reb
Price per Pair (US dollars $) Pair Genotype $384.80 Hemizygous for Tg(Stra8-cre)1Reb x Noncarrier $384.80 Noncarrier x Hemizygous for Tg(Stra8-cre)1Reb Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
|
|
|
Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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