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| These Gli1lz (or Gli1lacZ) mutant mice harbor a β-galactosidase "knock-in" mutation that also abolishes endogenous GLI-Kruppel family member GLI1 (Gli1) gene function, and may be useful for studying Hedgehog/Sonic Hedgehog signaling in axis patterning, proliferation, and cell fate specification of Hedgehog responding cells at different stages of embryogenesis. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System +/+ sibling x Heterozygote (Female x Male) 24-FEB-09 Species laboratory mouse Generation F?+ (03-JAN-11)
Generation DefinitionsDonating Investigator Dr. Alexandra L Joyner, Memorial Sloan-Kettering Cancer Center Description
Mice homozygous for the Gli1lz (or Gli1lacZ) allele are viable and semi-fertile, with a "knock-in" of β-galactosidase (lacZ) inserted into the first coding exon (exon 2) and replacing the genomic fragment encoding the entire N-terminal and zinc-finger domains of the targeted locus (exons 2-7); abolishing endogenous gene function even if alternative splicing occurs. Under control of the upstream promoter/enhancer elements, lacZ expression is observed in a pattern indistinguishable from wildtype gene mRNA expression. As Gli1 transcription is a readout of high level Hedgehog signaling, these Gli1lz (or Gli1lacZ) mice may be useful for studying Hedgehog/Sonic Hedgehog signaling in axis patterning, proliferation, and cell fate specification of Hedgehog responding cells at different stages of embryogenesis.Development
A targeting vector was designed to insert a nuclear localized β-galactosidase (lacZ) cDNA and loxP-flanked, reverse-oriented neo cassette into the first coding exon (exon 2) and delete the coding sequences in exons two to seven of the targeted gene. This construct was electroporated into 129S6/SvEvTac-derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with 129SvEv (Taconic) mice. Next, mutant mice were bred to TK-Cre transgenic mice (129SvEv genetic background) to remove the floxed neo cassette. The resulting Gli1lz (or Gli1lacZ) offspring were selectively bred to remove the TK-Cre transgene and then subsequently maintained by breeding to Swiss Webster mice and also interbreeding for many generations prior to arrival at The Jackson Laboratory. Upon arrival, mutant mice were bred to 129S1/SvImJ (Stock No. 002448) for at least one generation to establish the colony.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (255 strains)
007913 STOCK Gli1tm3(cre/ERT2)Alj/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
Gli1tm2Alj/Gli1+
involves: 129S6/SvEvTac * Black Swiss * Swiss Webster
- reproductive system phenotype
- *normal* reproductive system phenotype
- embryos show normal mammary gland development (MGI Ref ID J:112460)
Gli1tm2Alj/Gli1tm2Alj
involves: 129S6/SvEvTac * Black Swiss * Swiss Webster
- reproductive system phenotype
- *normal* reproductive system phenotype
- embryos show normal mammary gland development (MGI Ref ID J:112460)
This mouse can be used to support research in many areas including:
Developmental Biology Research
Limb Patterning Defects
Neurobiology Research
lacZ expression in neural tissue
Research Tools
lacZ Expression
Genetics Research
Tissue/Cell Markers
Tissue/Cell Markers: neurons
Neurobiology Research
cell marker
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Gli1tm2Alj | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Alexandra L Joyner | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | Gli1LacZ; Gli1lz; Gli1lzki; Gli1nlacZ; Gli1null; | ||
| Mutation Made By | Dr. Alexandra Joyner, Memorial Sloan-Kettering Cancer Center | ||
| Strain of Origin | 129S6/SvEvTac | ||
| ES Cell Line Name | W4 | ||
| ES Cell Line Strain | 129S6/SvEvTac | ||
| Site of Expression | LacZ expression replaces that of the targeted gene in a pattern indistinguishable from that of the wildtype. | ||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Molecular Note | The gene was disrupted by replacement of a genomic region encoding the Gli N-terminal and zinc finger domains with a floxed-neo cassette and a lacZ having a nuclear localizing signal. The floxed-neo cassette was subsequently removed by crossing mutant animals to a cre deleter strain. The lacZ expression pattern in homozygous mutant embryos is identical to that of wild-type Gli as determined by immunohistochemical analysis. [MGI Ref ID J:79392] | ||
| Gene Symbol and Name | Gli1, GLI-Kruppel family member GLI1 | ||
| Chromosome | 10 | ||
| Gene Common Name(s) | AV235269; GLI; Zfp-5; Zfp5; expressed sequence AV235269; zinc finger protein 5; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gli1 tm2 Alj MCA, Melt Curve Analysis
Gli1tm2AlJ, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Bai CB; Auerbach W; Lee JS; Stephen D; Joyner AL. 2002. Gli2, but not Gli1, is required for initial Shh signaling and ectopic activation of the Shh pathway. Development 129(20):4753-61. [PubMed: 12361967] [MGI Ref ID J:79392]
Additional References
Gli1tm2Alj relatedAhn S; Joyner AL. 2004. Dynamic changes in the response of cells to positive hedgehog signaling during mouse limb patterning. Cell 118(4):505-16. [PubMed: 15315762] [MGI Ref ID J:92507]
Bai CB; Stephen D; Joyner AL. 2004. All mouse ventral spinal cord patterning by hedgehog is Gli dependent and involves an activator function of Gli3. Dev Cell 6(1):103-15. [PubMed: 14723851] [MGI Ref ID J:87948]
Balordi F; Fishell G. 2007. Hedgehog signaling in the subventricular zone is required for both the maintenance of stem cells and the migration of newborn neurons. J Neurosci 27(22):5936-47. [PubMed: 17537964] [MGI Ref ID J:121967]
Balordi F; Fishell G. 2007. Mosaic removal of hedgehog signaling in the adult SVZ reveals that the residual wild-type stem cells have a limited capacity for self-renewal. J Neurosci 27(52):14248-59. [PubMed: 18160632] [MGI Ref ID J:130973]
Barsoum I; Yao HH. 2011. Redundant and differential roles of transcription factors gli1 and gli2 in the development of mouse fetal Leydig cells. Biol Reprod 84(5):894-9. [PubMed: 21209421] [MGI Ref ID J:173724]
Collins MA; Bednar F; Zhang Y; Brisset JC; Galban S; Galban CJ; Rakshit S; Flannagan KS; Adsay NV; Pasca di Magliano M. 2012. Oncogenic Kras is required for both the initiation and maintenance of pancreatic cancer in mice. J Clin Invest 122(2):639-53. [PubMed: 22232209] [MGI Ref ID J:184378]
Corrales JD; Blaess S; Mahoney EM; Joyner AL. 2006. The level of sonic hedgehog signaling regulates the complexity of cerebellar foliation. Development 133(9):1811-21. [PubMed: 16571625] [MGI Ref ID J:108507]
Corrales JD; Rocco GL; Blaess S; Guo Q; Joyner AL. 2004. Spatial pattern of sonic hedgehog signaling through Gli genes during cerebellum development. Development 131(22):5581-90. [PubMed: 15496441] [MGI Ref ID J:94461]
Dakubo GD; Mazerolle C; Furimsky M; Yu C; St-Jacques B; McMahon AP; Wallace VA. 2008. Indian hedgehog signaling from endothelial cells is required for sclera and retinal pigment epithelium development in the mouse eye. Dev Biol 320(1):242-55. [PubMed: 18582859] [MGI Ref ID J:139168]
Fabian SL; Penchev RR; St-Jacques B; Rao AN; Sipila P; West KA; McMahon AP; Humphreys BD. 2012. Hedgehog-Gli Pathway Activation during Kidney Fibrosis. Am J Pathol 180(4):1441-53. [PubMed: 22342522] [MGI Ref ID J:182155]
Gao J; Graves S; Koch U; Liu S; Jankovic V; Buonamici S; El Andaloussi A; Nimer SD; Kee BL; Taichman R; Radtke F; Aifantis I. 2009. Hedgehog signaling is dispensable for adult hematopoietic stem cell function. Cell Stem Cell 4(6):548-58. [PubMed: 19497283] [MGI Ref ID J:149820]
Garcia AD; Petrova R; Eng L; Joyner AL. 2010. Sonic hedgehog regulates discrete populations of astrocytes in the adult mouse forebrain. J Neurosci 30(41):13597-608. [PubMed: 20943901] [MGI Ref ID J:165495]
Hatsell SJ; Cowin P. 2006. Gli3-mediated repression of Hedgehog targets is required for normal mammary development. Development 133(18):3661-70. [PubMed: 16914490] [MGI Ref ID J:112460]
Hayes L; Ralls S; Wang H; Ahn S. 2013. Duration of Shh signaling contributes to mDA neuron diversity. Dev Biol 374(1):115-26. [PubMed: 23201023] [MGI Ref ID J:193109]
Huang CC; Miyagawa S; Matsumaru D; Parker KL; Yao HH. 2010. Progenitor cell expansion and organ size of mouse adrenal is regulated by sonic hedgehog. Endocrinology 151(3):1119-28. [PubMed: 20118198] [MGI Ref ID J:168522]
Ihrie RA; Shah JK; Harwell CC; Levine JH; Guinto CD; Lezameta M; Kriegstein AR; Alvarez-Buylla A. 2011. Persistent sonic hedgehog signaling in adult brain determines neural stem cell positional identity. Neuron 71(2):250-62. [PubMed: 21791285] [MGI Ref ID J:174685]
Kimura H; Stephen D; Joyner A; Curran T. 2005. Gli1 is important for medulloblastoma formation in Ptc1+/- mice. Oncogene 24(25):4026-36. [PubMed: 15806168] [MGI Ref ID J:99548]
King P; Paul A; Laufer E. 2009. Shh signaling regulates adrenocortical development and identifies progenitors of steroidogenic lineages. Proc Natl Acad Sci U S A 106(50):21185-90. [PubMed: 19955443] [MGI Ref ID J:155826]
Kolterud A; Grosse AS; Zacharias WJ; Walton KD; Kretovich KE; Madison BB; Waghray M; Ferris JE; Hu C; Merchant JL; Dlugosz AA; Kottmann AH; Gumucio DL. 2009. Paracrine Hedgehog signaling in stomach and intestine: new roles for hedgehog in gastrointestinal patterning. Gastroenterology 137(2):618-28. [PubMed: 19445942] [MGI Ref ID J:151258]
Li Binghua; Rauhauser AA; Dai J; Sakthivel R; Igarashi P; Jetten AM; Attanasio M. 2011. Increased hedgehog signaling in postnatal kidney results in aberrant activation of nephron developmental programs. Hum Mol Genet 20(21):4155-66. [PubMed: 21816948] [MGI Ref ID J:176685]
Li CY; Cha W; Luder HU; Charles RP; McMahon M; Mitsiadis TA; Klein OD. 2012. E-cadherin regulates the behavior and fate of epithelial stem cells and their progeny in the mouse incisor. Dev Biol 366(2):357-66. [PubMed: 22537490] [MGI Ref ID J:185423]
Li X; Udager AM; Hu C; Qiao XT; Richards N; Gumucio DL. 2009. Dynamic patterning at the pylorus: Formation of an epithelial intestine-stomach boundary in late fetal life. Dev Dyn 238(12):3205-3217. [PubMed: 19877272] [MGI Ref ID J:154372]
Liu J; Heydeck W; Zeng H; Liu A. 2012. Dual function of suppressor of fused in Hh pathway activation and mouse spinal cord patterning. Dev Biol 362(2):141-53. [PubMed: 22182519] [MGI Ref ID J:180775]
Machold R; Hayashi S; Rutlin M; Muzumdar MD; Nery S; Corbin JG; Gritli-Linde A; Dellovade T; Porter JA; Rubin LL; Dudek H; McMahon AP; Fishell G. 2003. Sonic hedgehog is required for progenitor cell maintenance in telencephalic stem cell niches. Neuron 39(6):937-50. [PubMed: 12971894] [MGI Ref ID J:85603]
Merchant A; Joseph G; Wang Q; Brennan S; Matsui W. 2010. Gli1 regulates the proliferation and differentiation of HSCs and myeloid progenitors. Blood 115(12):2391-6. [PubMed: 20107231] [MGI Ref ID J:159192]
Monje M; Mitra SS; Freret ME; Raveh TB; Kim J; Masek M; Attema JL; Li G; Haddix T; Edwards MS; Fisher PG; Weissman IL; Rowitch DH; Vogel H; Wong AJ; Beachy PA. 2011. Hedgehog-responsive candidate cell of origin for diffuse intrinsic pontine glioma. Proc Natl Acad Sci U S A 108(11):4453-8. [PubMed: 21368213] [MGI Ref ID J:170819]
Paul A; Laufer E. 2011. Endogenous biotin as a marker of adrenocortical cells with steroidogenic potential. Mol Cell Endocrinol 336(1-2):133-40. [PubMed: 21256921] [MGI Ref ID J:179464]
Saqui-Salces M; Coves-Datson E; Veniaminova NA; Waghray M; Syu LJ; Dlugosz AA; Merchant JL. 2012. Inflammation and Gli2 suppress gastrin gene expression in a murine model of antral hyperplasia. PLoS One 7(10):e48039. [PubMed: 23110168] [MGI Ref ID J:192305]
Seidel K; Ahn CP; Lyons D; Nee A; Ting K; Brownell I; Cao T; Carano RA; Curran T; Schober M; Fuchs E; Joyner A; Martin GR; de Sauvage FJ; Klein OD. 2010. Hedgehog signaling regulates the generation of ameloblast progenitors in the continuously growing mouse incisor. Development 137(22):3753-61. [PubMed: 20978073] [MGI Ref ID J:167067]
Sekiguchi H; Ii M; Jujo K; Renault MA; Thorne T; Clarke T; Ito A; Tanaka T; Klyachko E; Tabata Y; Hagiwara N; Losordo D. 2012. Estradiol triggers sonic-hedgehog-induced angiogenesis during peripheral nerve regeneration by downregulating hedgehog-interacting protein. Lab Invest 92(4):532-42. [PubMed: 22330336] [MGI Ref ID J:181988]
Shin K; Lee J; Guo N; Kim J; Lim A; Qu L; Mysorekar IU; Beachy PA. 2011. Hedgehog/Wnt feedback supports regenerative proliferation of epithelial stem cells in bladder. Nature 472(7341):110-4. [PubMed: 21389986] [MGI Ref ID J:170991]
Tripathi P; Guo Q; Wang Y; Coussens M; Liapis H; Jain S; Kuehn MR; Capecchi MR; Chen F. 2010. Midline signaling regulates kidney positioning but not nephrogenesis through Shh. Dev Biol 340(2):518-27. [PubMed: 20152829] [MGI Ref ID J:160256]
Walton KD; Kolterud A; Czerwinski MJ; Bell MJ; Prakash A; Kushwaha J; Grosse AS; Schnell S; Gumucio DL. 2012. Hedgehog-responsive mesenchymal clusters direct patterning and emergence of intestinal villi. Proc Natl Acad Sci U S A 109(39):15817-22. [PubMed: 23019366] [MGI Ref ID J:190319]
Yu K; McGlynn S; Matise MP. 2013. Floor plate-derived sonic hedgehog regulates glial and ependymal cell fates in the developing spinal cord. Development 140(7):1594-604. [PubMed: 23482494] [MGI Ref ID J:194893]
Yu W; Wang Y; McDonnell K; Stephen D; Bai CB. 2009. Patterning of ventral telencephalon requires positive function of Gli transcription factors. Dev Biol 334(1):264-75. [PubMed: 19632216] [MGI Ref ID J:153550]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number MGL375
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, heterozygous mice may be bred with wildtype siblings. The donating investigator reports that homozygous mice are viable but semi-fertile. Mating System +/+ sibling x Heterozygote (Female x Male) 24-FEB-09
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Heterozygous for Gli1tm2Alj
Price per Pair (US dollars $) Pair Genotype $296.00 Heterozygous for Gli1tm2Alj x Wild-type for Gli1tm2Alj $296.00 Wild-type for Gli1tm2Alj x Heterozygous for Gli1tm2Alj Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Heterozygous for Gli1tm2Alj
Price per Pair (US dollars $) Pair Genotype $384.80 Heterozygous for Gli1tm2Alj x Wild-type for Gli1tm2Alj $384.80 Wild-type for Gli1tm2Alj x Heterozygous for Gli1tm2Alj Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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