Strain Name: |
B6;129S4-Pou5f1tm2Jae/J |
|---|---|
Stock Number: |
008214 |
Availability: | Under Development for Distribution Colony |
| To register your interest in this strain go to the Strain Interest Form. |
Price and Supply Information | |
General Terms and Conditions |
| Former Name |
B6;129S4-Pou5f1tm2(EGFP)Jae/J (Changed: 28-FEB-08
) |
| Genes & Alleles | Pou5f1; Pou5f1tm2Jae; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Donating Investigator Rudolf Jaenisch, Whitehead Institute (MIT) Strain Description
Mice homozygous for this Oct4-EGFP mutation are viable and fertile. They harbor an IRES-EGFP fusion cassette downstream of the stop codon of the Oct4 (Pou5f1) gene. When treated with specific transcription factors (Oct4, Sox2, c-Myc and Klf4), some Oct4-EGFP murine embryonic fibroblasts (MEFs) have the properties of induced pluripotent stem (iPS) cells. Such iPS cells have the DNA methylation, gene expression and chromatin state of embryonic stem cells and can form viable chimeras, contribute to the germ line, and generate live late-term embryos when injected into tetraploid blastocysts. These Oct4-EGFP mutant mice may be useful for fluorescent labeling of embryonic stem cells, as well as for the selection of iPS cells (i.e. epigenetic reprogramming of somatic cells into pluripotent embryonic stem cells).Of note, these Oct4-EGFP mutant mice may also be used in conjunction with Oct4-neo mice (Stock No. 008204); a similar mutant strain bearing a neo selection cassette in the Oct4 locus.
Strain Development
A targeting vector was designed to insert an "IRES-EGFP-floxed NEO" cassette (containing an internal ribosomal entry site (IRES), enhanced green fluorescent protein (EGFP) sequence, and a loxP-flanked neo) between the stop codon and endogenous polyA signal of the targeted gene. This construct was electroporated into (C57BL/6 x 129S4Sv/Jae)F1-derived V6.5 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a cre expressing plasmid to remove the neo selection cassette. Chimeric mice were established. The resulting Oct4-EGFP mutant mice were maintained on a mixed B6;129S4 genetic background prior to arrival at The Jackson Laboratory.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Pou5f1tm2Jae | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Rudolf Jaenisch | ||
| Common Name(s) | Oct4-EGFP; Oct4-GFP; Oct4-IRES-GFP; Oct4-iresGFP; Pou5f1tm2(EGFP)Jae; | ||
| Mutation Made By | Rudolf Jaenisch, Whitehead Institute (MIT) | ||
| Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 | ||
| ES Cell Line Name | v6.5 | ||
| ES Cell Line Strain | (C57BL/6 x 129S4/SvJae)F1 | ||
| Gene Symbol and Name | Pou5f1, POU domain, class 5, transcription factor 1 | ||
| Chromosome | 17 | ||
| Gene Common Name(s) | MGC22487; OCT3; OCT4; OTF3; OTF4; Oct-3; Oct-3/4; Oct-4; Oct3/4; Otf-3; Otf-4; Otf3; Otf3-rs7; Otf3g; Otf4; octamer binding transcription factor 3; octamer binding transcription factor 3 related sequence 7; octamer binding transcription factor 4; | ||
| General Note | When treated with specific transcription factors (Oct4, Sox2, c-Myc and Klf4), some Oct4-EGFP murine embryonic fibroblasts (MEFs) have the properties of induced pluripotent stem (iPS) cells. Such iPS cells have the DNA methylation, gene expression and chromatin state of embryonic stem cells and can form viable chimeras, contribute to the germ line, and generate live late-term embryos when injected into tetraploid blastocysts. | ||
| Molecular Note | A targeting vector was designed to insert an "IRES-EGFP-floxed NEO" cassette (containing an internal ribosomal entry site (IRES), enhanced green fluorescent protein (EGFP) sequence, and a loxP-flanked neo) between the stop codon and endogenous polyA signal of the targeted gene. This construct was electroporated into (C57BL/6 x 129S4Sv/Jae)F1-derived V6.5 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a cre expressing plasmid to remove the neo selection cassette. [J:130248] | ||
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 101043 B6129SF1/J | (approximate) | |
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, homozygous mice may be bred. |
|---|
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (138 strains)
006911 B6;129-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm2(tetO-Pou5f1)Jae/J 008204 B6;129S4-Pou5f1tm1Jae/J 004654 B6;CBA-Tg(Pou5f1-EGFP)2Mnn/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Research Applications
This mouse can be used to support research in many areas including:
Research Tools
Cell Biology Research
Developmental Biology Research
Fluorescent Proteins
General Purpose
Genetics Research (Mutagenesis and Transgenesis: Production of Targeted Mutations ("Knockouts"))
Genetics Research (Mutagenesis and Transgenesis: Production of Transgenic Mice)
Genetics Research (Mutagenesis and Transgenesis: Production of Transgenics)
Genetics Research (Tissue/Cell Markers)
References
Selected Reference(s)
Additional ReferencesLengner CJ; Camargo FD; Hochedlinger K; Welstead GG; Zaidi S; Gokhale S; Scholer HR; Tomilin A; Jaenisch R. 2007. Oct4 expression is not required for mouse somatic stem cell self-renewal. Cell Stem Cell 1(4):403-415. [PubMed: 18159219] [J:130248]
Price and Supply Information
| Strain Name: | B6;129S4-Pou5f1tm2Jae/J |
| Stock Number: | 008214 |
This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date:
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
View All Strains Under Development
Supply Details
| Standard Supply | Under Development for Distribution Colony |
|---|---|
| Supply Notes |
This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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