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Genes & Alleles

Car14;   Car14tm1Sly;

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Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Donating Investigator William Sly,   Saint Louis University Medical Center

Strain Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA or protein) is detected by RT-PCR or Western blot analysis of brain, heart, kidney, liver, and muscle tissue. Homozygotes exhibit reduced flash electroretinograms (rod/cone a-wave, b-wave, and cone b-wave) when compared to wildtype controls. The abnormal retinal light response of homozygotes is stable over time. This mutant mouse strain may be useful in studies of extracellular retinal pH and carbon dioxide regulation, photoreceptor response and retinal function.

Strain Development
A tACE-CRE-neo^r targeting vector containing neomycin resistance, cre recombinase and the mouse Ace (angiotensin I converting enzyme (peptidyl-dipeptidase A) 1) genes was used to disrupt exon 4 (which encodes 2 active-site His residues), 16 bp of intron 3, and 13 bp of intron 4, resulting in early termination in exon 5. The construct was electroporated into 129S1/Sv-Oca2⁺ Tyr⁺ Kitl^Sl-J/J derived W9.5s embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The self-excising targeting vector is removed during spermatogenesis in the male chimeric animals, leaving one loxP site. The mice were then backcrossed to C57BL/6 for 20 generations before arriving at The Jackson Laboratory.

Mammalian Phenotype Terms assigned by genotype The following phenotype information may relate to a genetic background differing from this JAX® Mice strain. Car14tm1Sly/Car14tm1Sly         involves: 129S1/Sv * C57BL/6 normal phenotype no abnormal phenotype detected (MGI Ref ID J:103742) homozygous mice at near expected Mendelian ratios normal growth, health, and fertility

Gene & Allele Details

Allele Symbol		Car14tm1Sly
Allele Name		targeted mutation 1, William S Sly
Common Name(s)		CA XIV KO; CAXIVdl1Sws;
Mutation Made By		William Sly,   Saint Louis University Medical Center
Strain of Origin		129S1/Sv-Oca2+ Tyr+ KitlSl-J/J
ES Cell Line Name		Other (see notes)
ES Cell Line Strain		129
Gene Symbol and Name		Car14, carbonic anhydrase 14
Chromosome		3
Gene Common Name(s)		AW536446; CA XIV; expressed sequence AW536446;
General Note		ES cell line = W9.5s
Molecular Note		A targeting vector was used to replace exon 4, 16 bp of intron 3, and 13 bp of intron 4 with the self-excising tACE-CRE-neo cassette. The frameshift resulting from recombination putatively results in early termination in exon 5. RT-PCR failed to detect the predicted 0.75 fragment in brain, heart, kidney, liver or muscle samples. Protein was not detected in Western blot analysis of the same tissues. [MGI Ref ID J:103742]

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Genotyping Protocols

Car14^tm1Sly

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as homozygotes.

Additional Web Information

Congenic Nomenclature

Research Applications

This mouse can be used to support research in many areas including:

Sensorineural Research
Eye Defects

References

Selected Reference(s)

Shah GN; Ulmasov B; Waheed A; Becker T; Makani S; Svichar N; Chesler M; Sly WS. 2005. Carbonic anhydrase IV and XIV knockout mice: roles of the respective carbonic anhydrases in buffering the extracellular space in brain. Proc Natl Acad Sci U S A 102(46):16771-6. [PubMed: 16260723]  [MGI Ref ID J:103742]

Additional References

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Price and Supply Information

Strain Name:	B6.129S1-Car14tm1Sly/J
Stock Number:	008218

 

This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.

Estimated Available for Sale Date:

Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.

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Supply Details

Standard Supply	Under Development for Distribution Colony
Supply Notes	This strain is included in the Induced Mutant Resource Colony collection.
Licensing	See General Terms and Conditions below
Control Information	View Control Information in Strain Details.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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