Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse   Donating Investigator Martin Matzuk,   Baylor College of Medicine

Description
Female mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygous males are subfertile with approximately 20% reduction in average litter size. Heterozygous males have normal fertility and fecundity. Homozygous males have slightly abnormal sperm morphology. Although sperm from homozygotes bind to oocyte zona pellucida normally, the slower in vitro fertilization rate indicates reduced zona pellucida penetration. No gene product (mRNA or protein) is detected by Northern or Western blot analysis or testicular tissue and sperm. This mutant mouse strain may be useful in studies of spermiogenesis and sperm-egg interaction.

Development
A targeting vector containing a PgkHPRT cassette was used to disrupt exons 1, 2 and 3, which encode the signal peptide region. The construct was electroporated into 129S7/SvEvBrd-Hprt1^b-m2 derived AB2.2 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting male chimeric animals were crossed to C57BL/6J female mice. The mice were maintained on a B6;129S7 background before arriving at The Jackson Laboratory.

Control Information

	Control
	Wild-type from the colony
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Zpbp2^tm1Zuk/Zpbp2^tm1Zuk

        involves: 129S7/SvEvBrd * C57BL/6J

• reproductive system phenotype
• *normal* reproductive system phenotype
  • total testis weight is similar to wild-type and heterozygous littermates   (MGI Ref ID J:126661)
• • delayed fertilization
    • in vitro fertilization rates with zona-intact eggs are slower relative to rates for heterozygous males   (MGI Ref ID J:126661)
  • reduced male fertility
    • decreased litter size in crosses to heterozygous females relative to crosses of homozygous females to heterozygous males and heterozygous crosses   (MGI Ref ID J:126661)
    • however, female fertility is normal   (MGI Ref ID J:126661)
  • teratozoospermia
    • subtle deformities are seen including; a shortened apical hook, smaller apical angle, and bulges composed of excessive acrosome folded onto itself   (MGI Ref ID J:126661)
    • however, sperm counts and motility are similar to heterozygous males   (MGI Ref ID J:126661)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Reproductive Biology Research
Fertility Defects
      males only

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Zpbp2tm1Zuk
Allele Name		targeted mutation 1, Martin M Matzuk
Allele Type		Targeted (knock-out)
Mutation Made By		Yi-Nan Lin,   IBMS, Academia Sinica
Strain of Origin		129S7/SvEvBrd-Hprt
ES Cell Line Name		AB2.2
ES Cell Line Strain		129S7/SvEvBrd-Hprt
Gene Symbol and Name		Zpbp2, zona pellucida binding protein 2
Chromosome		11
Gene Common Name(s)		1700017D11Rik; RIKEN cDNA 1700017D11 gene; ZPBPL;
Molecular Note		Exons 1, 2 and 3 were replaced with a PgkHPRT cassette via homologous recombination. This resulted in deletion of the signal peptide encoding region. Northern and Western blot analysis confirmed the absence of RNA transcript and protein in the testes. [MGI Ref ID J:126661]

Genotyping

Genotyping Information

Genotyping Protocols

Zpbp2^tm1Zuk, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Lin YN; Roy A; Yan W; Burns KH; Matzuk MM. 2007. Loss of zona pellucida binding proteins in the acrosomal matrix disrupts acrosome biogenesis and sperm morphogenesis. Mol Cell Biol 27(19):6794-805. [PubMed: 17664285]  [MGI Ref ID J:126661]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as homozygous females and heterozygous males. Homozygous females are fertile, homozygous males are subfertile (approximately 20% reduction in average litter size).

Purchasing information

Pricing, Supply Level & Notes, Controls

General Supply Notes

• This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	Wild-type from the colony
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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