Strain Name:

B6;FVB-Tg(IGFBP2)1Miel/J

Stock Number:

008222

Order this mouse

Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
These IGFBP-2 overexpressing transgenic mice may be useful in studying metabolic homeostasis, adipocyte biology, and the role of insulin-like growth factor binding protein in protecting against obesity- and age-associated complications (such as hypertension and diabetes).

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6.FVB-Tg(IGFBP2)1Miel/J    (Changed: 26-AUG-14 )
Type Congenic; Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
 
Donating Investigator Ajay Shah,   King's College London

Description
Mice hemizygous for the IGFBP-2 transgene are viable and fertile with no reported gross morphological or developmental changes. Transgenic mice overexpress human IGFBP-2 (hIGFBP-2), with hIGFBP-2 mRNA detected in a variety of organs and tissues, including adipose tissue. Overexpression of hIGFBP-2 is associated with reduced susceptibility to obesity and improved insulin sensitivity; transgenic mice are protected from glucose intolerance and increased blood pressure with age, and are also resistant to obesity and insulin resistance on a high fat diet. The phenotype of hIGFBP-2 overexpressing mice may vary between male and female mice. These IGFBP-2 transgenic mice may be useful in studying metabolic homeostasis, adipocyte biology, and the role of insulin-like growth factor binding protein in protecting against obesity- and age-associated complications (such as hypertension and diabetes).

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. As these mice were originally characterized on the FVB/N genetic background, it should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development
The IGFBP-2 transgene (isolated from a human cosmid clone) contains the entire structural human IGFBP2 gene. This 39 kb transgene was microinjected into the pronucleus of FVB/N embryos. Founder mice (line tg1) were bred to FVB/N mice. After this, the Donating Investigator reports that the IGFBP-2 transgenic mice were backcrossed to C57BL/6J mice for at least 9 generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were additionally backcrossed to C57BL/6J for at least one generation. SNP (single nucleotide polymorphism) panel analysis performed by The Jackson Laboratory revealed these mice are approximately 94% congenic on a C57BL/6 genetic background, which is less than the expected incipient congenic background.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Tg(IGFBP2)1Miel/0

        FVB/N-Tg(IGFBP2)1Miel
  • growth/size/body phenotype
  • decreased susceptibility to diet-induced obesity
    • on high-fat diet for 32 weeks, mutants remain lean with no significant increase in size of fat depots, whereas wild-type mice have markedly increased size of fat depots when examined at 40 weeks   (MGI Ref ID J:121961)
  • decreased total body fat amount
    • on high-fat diet for 32 weeks, mutants have smaller fat depots than wild-type mice on the same diet when examined at 40 weeks   (MGI Ref ID J:121961)
  • slow postnatal weight gain
    • on a high-fat/high-calorie diet for 32 weeks from 8 weeks of age, mutants show attenuated weight gain relative to wild-type mice   (MGI Ref ID J:121961)
  • adipose tissue phenotype
  • abnormal abdominal fat pad morphology
    • transgenic mice on high-fat diet for 32 weeks have significant reduction intraabdominal fat than wild-type mice; similarly, regular chow-fed mutants have less abdominal fat than diet-matched controls   (MGI Ref ID J:121961)
  • abnormal fat cell morphology
    • adipocytes are smaller than in wild-type mice, on both regular (1390 vs. 2100 um2 mean cell area) and high-fat chow diets (2800 vs. 5900 um2 mean cell area)   (MGI Ref ID J:121961)
  • decreased total body fat amount
    • on high-fat diet for 32 weeks, mutants have smaller fat depots than wild-type mice on the same diet when examined at 40 weeks   (MGI Ref ID J:121961)
  • cardiovascular system phenotype
  • *normal* cardiovascular system phenotype
    • mutants are protected from age-related hypertension observed in aged wild-type mice   (MGI Ref ID J:121961)
    • decreased systemic arterial systolic blood pressure
      • wild-type mice at 40 weeks show increased systolic blood pressure compared to transgenic mice on both regular chow and high-fat diets   (MGI Ref ID J:121961)
  • liver/biliary system phenotype
  • *normal* liver/biliary system phenotype
    • on high-fat diet for 32 weeks, mutants exhibit lower liver weight relative to controls when examined at 40 weeks   (MGI Ref ID J:121961)
    • on high-fat diet for 32 weeks, mutants do not exhibit steatosis, whereas wild-type livers have macroscopic appearance compatible with increased fat content   (MGI Ref ID J:121961)
  • homeostasis/metabolism phenotype
  • *normal* homeostasis/metabolism phenotype
    • at 8 weeks of age, blood glucose and insulin levels are similar to wild-type mice   (MGI Ref ID J:121961)
    • insulin levels are similar to wild-type at 4-6 months of age   (MGI Ref ID J:121961)
    • on high-fat diet, total energy intake is not significantly different from wild-type   (MGI Ref ID J:121961)
    • abnormal glucose homeostasis
      • in response to IGF-I, transgenic mice show an attenuated hypoglycemic response; plasma glucose levels decrease by ~half the value exhibited by wild-type mice   (MGI Ref ID J:121961)
      • abnormal circulating insulin level
        • in response to carbohydrate challenge, no siginificant increase in plasma insulin level is observed compared to elevated levels displayed by wild-type mice   (MGI Ref ID J:121961)
      • decreased circulating glucose level
        • by 4-6 months of age, transgenic mice display lower nonfasted glucose levels than wild-type littermates   (MGI Ref ID J:121961)
        • increase in blood glucose of transgenic mice on high-fat diet is significantly blunted relative to wild-type   (MGI Ref ID J:121961)
      • improved glucose tolerance
        • mice are protected against the age-related decline in glucose tolerance displayed by wild-type mice between 8 and 40 weeks   (MGI Ref ID J:121961)
        • on high-fat diet, transgenic mice are protected from development of glucose intolerance compared to wild-type   (MGI Ref ID J:121961)
        • on both regular and high-fat diets, blood glucose of transgenic mice is significantly lower after glucose tolerance test than in wild-type   (MGI Ref ID J:121961)
      • increased insulin sensitivity
        • hypoglycemic response to exogenous insulin is greater in 40-week old mice compared to controls   (MGI Ref ID J:121961)
    • decreased circulating leptin level
      • leptin level increase is blunted in transgenic mice on high-fat diet, compared to wild-type   (MGI Ref ID J:121961)
    • decreased circulating triglyceride level
      • on high-fat diet, plasma triglyceride levels are significantly lower than in wild-type   (MGI Ref ID J:121961)
    • decreased susceptibility to diet-induced obesity
      • on high-fat diet for 32 weeks, mutants remain lean with no significant increase in size of fat depots, whereas wild-type mice have markedly increased size of fat depots when examined at 40 weeks   (MGI Ref ID J:121961)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cardiovascular Research
Other

Diabetes and Obesity Research
Insulin Receptors and Growth Factors
Obesity With Diabetes
Obesity Without Diabetes
Type 2 Diabetes (NIDDM)

Metabolism Research

Research Tools
Cardiovascular Research
Diabetes and Obesity Research
Metabolism Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(IGFBP2)1Miel
Allele Name transgene insertion 1, John Miell
Allele Type Transgenic (Inserted expressed sequence)
Common Name(s) line tg1;
Mutation Made By John Miell,   University Hospital Lewisham
Strain of OriginFVB/N
Expressed Gene IGFBP2, insulin-like growth factor binding protein 2, 36kDa, human
Promoter IGFBP2, insulin-like growth factor binding protein 2, 36kDa, human
Molecular Note The IGFBP-2 transgene (isolated from a human cosmid clone) contains the entire structural human IGFBP2 gene. This 39 kb transgene was microinjected into the pronucleus of FVB/N embryos. Two lines tg1 and tg2 were established and line tg1 was analyzed in detail. [MGI Ref ID J:121961]
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(IGFBP2)1Miel MCA, Melt Curve Analysis
Tg(IGFBP2)1Miel, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Wheatcroft SB; Kearney MT; Shah AM; Ezzat VA; Miell JR; Modo M; Williams SC; Cawthorn WP; Medina-Gomez G; Vidal-Puig A; Sethi JK; Crossey PA. 2007. IGF-binding protein-2 protects against the development of obesity and insulin resistance. Diabetes 56(2):285-94. [PubMed: 17259371]  [MGI Ref ID J:121961]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, carrier mice may be bred to wildtype or C57BL/6J inbred mice.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.6)