Strain Name: |
NOD.129S2(B6)-Cd74tm1Doi/LwnJ |
|---|---|
Stock Number: |
008224 |
Availability: | Repository- Live |
General Terms and Conditions |
| Strain Common Names | NOD.Ii; |
| Genes & Alleles | Cd74; Cd74tm1Doi; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donor Strain 129S2 Donating Investigator Li Wen, Yale School of Medicine H2 Haplotype g7 Generation N12F20+F1 (08-FEB-08) Strain Description
Mice homozygous for the Cd74 targeted mutation are viable and fertile. Cd74 deficiency affects CD4 T cell development; therefore these mice have very few CD4+ T cells. Antigen presenting cells have reduced, though not abolished, ability to present MHC class II antigens, such as GAD. These mice are protected from diabetes. This stock is important for investigation of MHC class II molecule associated self-antigen selection and the importance of self-peptide in CD4 T cell selection and development.Strain Development
Cd74, antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated), located on Chromosome 18, 32cM is commonly known as Ii (Ia-associated invariant chain). Cd74 acts as an essential chaperone to promote MHC class II surface expression, Ag presentation, and selection of CD4+ T cells. A neomycin resistant cassette replaced exon 2 and 3 of the Cd74 gene. The B6.129S2-Cd74Tm1Doi was crossed to NOD/Caj for 12 generations prior to making homozygous for the mutation. In 2008, the T1DR received this strain at N12F20.
Gene & Allele Details
| Allele Symbol | Cd74tm1Doi | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Christophe Benoist and Diane Mathis | ||
| Common Name(s) | Cd74tm1Dim; Ii-; Ii0; | ||
| Mutation Made By | Christophe Benoist, Joslin Diabetes Center | ||
| Strain of Origin | 129S2/SvPas | ||
| ES Cell Line Name | D3 | ||
| ES Cell Line Strain | 129S2/SvPas | ||
| Gene Symbol and Name | Cd74, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) | ||
| Chromosome | 18 | ||
| Gene Common Name(s) | CLIP; DHLAG; HLA-DR-GAMMA; HLADG; INVG34; Ia-GAMMA; Ia-associated invariant chain; Ii; | ||
| Molecular Note | Replacement of exons 2 and 3 with a neomycin resistance gene. [MGI Ref ID J:4119] | ||
Control Information
| Control | ||
|---|---|---|
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Related Strains
Strains carrying other alleles of Cd74
002729 B6.129S6-Cd74tm1Liz/J 002730 CAn.129S6(B6)-Cd74tm1Liz/J View Strains carrying other alleles of Cd74 (2 strains)
Additional Web Information
Congenic Nomenclature
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
Diabetes and Obesity Research
Type 1 Diabetes (IDDM) (resistant)
Immunology and Inflammation Research
Autoimmunity (Type 1 Diabetes)
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Research Tools
Immunology and Inflammation Research (specific T cell deficiency)
References
Selected Reference(s)
Additional ReferencesViville S; Neefjes J; Lotteau V; Dierich A; Lemeur M; Ploegh H; Benoist C; Mathis D. 1993. Mice lacking the MHC class II-associated invariant chain. Cell 72(4):635-48. [PubMed: 7679955] [MGI Ref ID J:4119]
Price and Supply Information
| Strain Name: | NOD.129S2(B6)-Cd74tm1Doi/LwnJ |
| Stock Number: | 008224 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Type 1 Diabetes Repository collection. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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