Strain Name:

C57BL/6-Tg(Ckm-Ppargc1a)31Brsp/J

Stock Number:

008231

Order this mouse

Availability:

Cryopreserved - Ready for recovery

These MCK-PGC1alpha transgenic mice express mouse peroxisome proliferative activated receptor, gamma, coactivator 1 alpha under the direction of the mouse muscle creatine kinase promoter. Muscle fibers from transgenic mice exhibit a more type II oxidative phenotype than wild-type. This mutant mouse strain may be useful in studies of muscle physiology and disease, exercise and oxidative capacity, and metabolic homeostasis.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Coisogenic; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating Investigator Bruce Spiegelman,   Dana-Farber Cancer Institute

Description
These transgenic mice express mouse peroxisome proliferative activated receptor, gamma, coactivator 1 alpha under the direction of the mouse muscle creatine kinase promoter. Transgene expression is specific to skeletal muscle. The increased levels of the transgene product induces expression of energy and mitochondrial oxidative metabolism genes such as cytochrome c oxidase II and IV. Muscle fibers from transgenic mice exhibit a more type II oxidative phenotype than wildtype mice. Isolated extensor digitorum longus muscle from transgenic mice exhibit increased fatigue resistance when compared to wildtype. Muscle fiber diameter loss in response to denervation and fasting is less severe in transgenic animals when compared to controls. Mice that are hemizygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of muscle physiology and disease, exercise and oxidative capacity, and metabolic homeostasis.

Development
A transgenic construct containing the mouse peroxisome proliferative activated receptor, gamma, coactivator 1 alpha gene under the control of the mouse muscle creatine kinase promoter was injected into fertilized C57BL/6 mouse oocytes. Founder line 31 was subsequently established.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of Ckm     (11 strains)

Strains carrying other alleles of Ppargc1a
008597   B6.129-Ppargc1atm1Brsp/J
009666   B6.129-Ppargc1atm2.1Brsp/J
009662   B6.129X1-Ppargc1atm1Dpk/J
012387   FVB-Tg(tetO-Ppargc1a)1Dpk/J
View Strains carrying other alleles of Ppargc1a     (4 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Tg(Ckm-Ppargc1a)31Brsp/0

        C57BL/6-Tg(Ckm-Ppargc1a)31Brsp
  • muscle phenotype
  • abnormal muscle physiology
    • muscles in mutant animals are able to sustain a longer duration of continuous contraction; isolated extensor digitorum longus (EDL) muscles show much greater fatigue resistance compared to control muscle   (MGI Ref ID J:78333)
    • muscle fiber diameter loss in response to denervation and fasting is less severe in transgenic animals when compared to controls   (MGI Ref ID J:78333)
  • abnormal skeletal muscle morphology
    • most muscles in transgenic animals display a red color characteristic of oxidative muscle; in contrast, control muscles are paler in appearance   (MGI Ref ID J:78333)

Tg(Ckm-Ppargc1a)31Brsp/0

        C57BL/6-Tg(Ckm-Ppargc1a)31Brsp/J
  • cellular phenotype
  • abnormal aerobic respiration
    • mitochondria in muscle cells exhibit increased cellular respiration compared with wild-type cells   (MGI Ref ID J:176080)
  • increased mitochondrial proliferation
    • muscle cells contain increased mitochondrial DNA compared with control cells   (MGI Ref ID J:176080)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Metabolism Research

Research Tools
Metabolism Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(Ckm-Ppargc1a)31Brsp
Allele Name transgene insertion 31, Bruce M Spiegelman
Allele Type Transgenic (Inserted expressed sequence)
Common Name(s) MCK-PGC-1a; MCK-PGC-1a tg; MPGC-1alpha TG; mck-PGC-1alpha1;
Mutation Made By Bruce Spiegelman,   Dana-Farber Cancer Institute
Strain of OriginC57BL/6
Expressed Gene Ppargc1a, peroxisome proliferative activated receptor, gamma, coactivator 1 alpha, mouse, laboratory
Promoter Ckm, creatine kinase, muscle, mouse, laboratory
Molecular Note A transgenic construct containing the mouse peroxisome proliferative activated receptor, gamma, coactivator 1 alpha gene under the control of the mouse muscle creatine kinase promoter was injected into fertilized C57BL/6 mouse oocytes. Founder line 31 was subsequently established. [MGI Ref ID J:78333]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(Ckm-Ppargc1a)31Brsp STD PCR, Melt Curve Analysis
Tg(Ckm-Ppargc1a)31Brsp STD PCR, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Lin J; Wu H; Tarr PT; Zhang CY; Wu Z; Boss O; Michael LF; Puigserver P; Isotani E; Olson EN; Lowell BB; Bassel-Duby R; Spiegelman BM. 2002. Transcriptional co-activator PGC-1 alpha drives the formation of slow-twitch muscle fibres. Nature 418(6899):797-801. [PubMed: 12181572]  [MGI Ref ID J:78333]

Additional References

Tg(Ckm-Ppargc1a)31Brsp related

Agudelo LZ; Femenia T; Orhan F; Porsmyr-Palmertz M; Goiny M; Martinez-Redondo V; Correia JC; Izadi M; Bhat M; Schuppe-Koistinen I; Pettersson AT; Ferreira DM; Krook A; Barres R; Zierath JR; Erhardt S; Lindskog M; Ruas JL. 2014. Skeletal Muscle PGC-1alpha1 Modulates Kynurenine Metabolism and Mediates Resilience to Stress-Induced Depression. Cell 159(1):33-45. [PubMed: 25259918]  [MGI Ref ID J:213551]

Arnold AS; Gill J; Christe M; Ruiz R; McGuirk S; St-Pierre J; Tabares L; Handschin C. 2014. Morphological and functional remodelling of the neuromuscular junction by skeletal muscle PGC-1alpha. Nat Commun 5:3569. [PubMed: 24686533]  [MGI Ref ID J:211186]

Austin S; Klimcakova E; St-Pierre J. 2011. Impact of PGC-1alpha on the topology and rate of superoxide production by the mitochondrial electron transport chain. Free Radic Biol Med 51(12):2243-8. [PubMed: 21964033]  [MGI Ref ID J:179298]

Chakkalakal JV; Nishimune H; Ruas JL; Spiegelman BM; Sanes JR. 2010. Retrograde influence of muscle fibers on their innervation revealed by a novel marker for slow motoneurons. Development 137(20):3489-99. [PubMed: 20843861]  [MGI Ref ID J:165805]

Chinsomboon J; Ruas J; Gupta RK; Thom R; Shoag J; Rowe GC; Sawada N; Raghuram S; Arany Z. 2009. The transcriptional coactivator PGC-1alpha mediates exercise-induced angiogenesis in skeletal muscle. Proc Natl Acad Sci U S A 106(50):21401-6. [PubMed: 19966219]  [MGI Ref ID J:155818]

Choi CS; Befroy DE; Codella R; Kim S; Reznick RM; Hwang YJ; Liu ZX; Lee HY; Distefano A; Samuel VT; Zhang D; Cline GW; Handschin C; Lin J; Petersen KF; Spiegelman BM; Shulman GI. 2008. Paradoxical effects of increased expression of PGC-1alpha on muscle mitochondrial function and insulin-stimulated muscle glucose metabolism. Proc Natl Acad Sci U S A 105(50):19926-31. [PubMed: 19066218]  [MGI Ref ID J:142666]

Da Cruz S; Parone PA; Lopes VS; Lillo C; McAlonis-Downes M; Lee SK; Vetto AP; Petrosyan S; Marsala M; Murphy AN; Williams DS; Spiegelman BM; Cleveland DW. 2012. Elevated PGC-1alpha activity sustains mitochondrial biogenesis and muscle function without extending survival in a mouse model of inherited ALS. Cell Metab 15(5):778-86. [PubMed: 22560226]  [MGI Ref ID J:184777]

Dillon LM; Williams SL; Hida A; Peacock JD; Prolla TA; Lincoln J; Moraes CT. 2012. Increased mitochondrial biogenesis in muscle improves aging phenotypes in the mtDNA mutator mouse. Hum Mol Genet 21(10):2288-97. [PubMed: 22357654]  [MGI Ref ID J:183779]

Elias BC; Mathew S; Srichai MB; Palamuttam R; Bulus N; Mernaugh G; Singh AB; Sanders CR; Harris RC; Pozzi A; Zent R. 2014. The integrin beta1 subunit regulates paracellular permeability of kidney proximal tubule cells. J Biol Chem 289(12):8532-44. [PubMed: 24509849]  [MGI Ref ID J:212444]

Geng T; Li P; Yin X; Yan Z. 2011. PGC-1alpha Promotes Nitric Oxide Antioxidant Defenses and Inhibits FOXO Signaling Against Cardiac Cachexia in Mice. Am J Pathol 178(4):1738-48. [PubMed: 21435455]  [MGI Ref ID J:169847]

Hindi SM; Mishra V; Bhatnagar S; Tajrishi MM; Ogura Y; Yan Z; Burkly LC; Zheng TS; Kumar A. 2014. Regulatory circuitry of TWEAK-Fn14 system and PGC-1alpha in skeletal muscle atrophy program. FASEB J 28(3):1398-411. [PubMed: 24327607]  [MGI Ref ID J:210689]

Kiilerich K; Adser H; Jakobsen AH; Pedersen PA; Hardie DG; Wojtaszewski JF; Pilegaard H. 2010. PGC-1{alpha} increases PDH content but does not change acute PDH regulation in mouse skeletal muscle. Am J Physiol Regul Integr Comp Physiol 299(5):R1350-9. [PubMed: 20720174]  [MGI Ref ID J:165597]

Lira VA; Okutsu M; Zhang M; Greene NP; Laker RC; Breen DS; Hoehn KL; Yan Z. 2013. Autophagy is required for exercise training-induced skeletal muscle adaptation and improvement of physical performance. FASEB J 27(10):4184-4193. [PubMed: 23825228]  [MGI Ref ID J:201170]

McDermott-Roe C; Ye J; Ahmed R; Sun XM; Serafin A; Ware J; Bottolo L; Muckett P; Canas X; Zhang J; Rowe GC; Buchan R; Lu H; Braithwaite A; Mancini M; Hauton D; Marti R; Garcia-Arumi E; Hubner N; Jacob H; Serikawa T; Zidek V; Papousek F; Kolar F; Cardona M; Ruiz-Meana M; Garcia-Dorado D; Comella JX; Felkin LE; Barton PJ; Arany Z; Pravenec M; Petretto E; Sanchis D; Cook SA. 2011. Endonuclease G is a novel determinant of cardiac hypertrophy and mitochondrial function. Nature 478(7367):114-8. [PubMed: 21979051]  [MGI Ref ID J:177433]

Perez-Schindler J; Summermatter S; Salatino S; Zorzato F; Beer M; Balwierz PJ; van Nimwegen E; Feige JN; Auwerx J; Handschin C. 2012. The corepressor NCoR1 antagonizes PGC-1alpha and estrogen-related receptor alpha in the regulation of skeletal muscle function and oxidative metabolism. Mol Cell Biol 32(24):4913-24. [PubMed: 23028049]  [MGI Ref ID J:192729]

Perez-Schindler J; Summermatter S; Santos G; Zorzato F; Handschin C. 2013. The transcriptional coactivator PGC-1alpha is dispensable for chronic overload-induced skeletal muscle hypertrophy and metabolic remodeling. Proc Natl Acad Sci U S A 110(50):20314-9. [PubMed: 24277823]  [MGI Ref ID J:205184]

Roberts LD; Bostrom P; O'Sullivan JF; Schinzel RT; Lewis GD; Dejam A; Lee YK; Palma MJ; Calhoun S; Georgiadi A; Chen MH; Ramachandran VS; Larson MG; Bouchard C; Rankinen T; Souza AL; Clish CB; Wang TJ; Estall JL; Soukas AA; Cowan CA; Spiegelman BM; Gerszten RE. 2014. beta-Aminoisobutyric acid induces browning of white fat and hepatic beta-oxidation and is inversely correlated with cardiometabolic risk factors. Cell Metab 19(1):96-108. [PubMed: 24411942]  [MGI Ref ID J:210544]

Romanino K; Mazelin L; Albert V; Conjard-Duplany A; Lin S; Bentzinger CF; Handschin C; Puigserver P; Zorzato F; Schaeffer L; Gangloff YG; Ruegg MA. 2011. Myopathy caused by mammalian target of rapamycin complex 1 (mTORC1) inactivation is not reversed by restoring mitochondrial function. Proc Natl Acad Sci U S A 108(51):20808-13. [PubMed: 22143799]  [MGI Ref ID J:180519]

Summermatter S; Santos G; Perez-Schindler J; Handschin C. 2013. Skeletal muscle PGC-1alpha controls whole-body lactate homeostasis through estrogen-related receptor alpha-dependent activation of LDH B and repression of LDH A. Proc Natl Acad Sci U S A 110(21):8738-43. [PubMed: 23650363]  [MGI Ref ID J:197316]

Summermatter S; Shui G; Maag D; Santos G; Wenk MR; Handschin C. 2013. PGC-1alpha improves glucose homeostasis in skeletal muscle in an activity-dependent manner. Diabetes 62(1):85-95. [PubMed: 23086035]  [MGI Ref ID J:208612]

Summermatter S; Thurnheer R; Santos G; Mosca B; Baum O; Treves S; Hoppeler H; Zorzato F; Handschin C. 2012. Remodeling of calcium handling in skeletal muscle through PGC-1alpha: impact on force, fatigability, and fiber type. Am J Physiol Cell Physiol 302(1):C88-99. [PubMed: 21918181]  [MGI Ref ID J:180636]

Summermatter S; Troxler H; Santos G; Handschin C. 2011. Coordinated balancing of muscle oxidative metabolism through PGC-1alpha increases metabolic flexibility and preserves insulin sensitivity. Biochem Biophys Res Commun 408(1):180-5. [PubMed: 21501593]  [MGI Ref ID J:172051]

Takikita S; Schreiner C; Baum R; Xie T; Ralston E; Plotz PH; Raben N. 2010. Fiber type conversion by PGC-1alpha activates lysosomal and autophagosomal biogenesis in both unaffected and Pompe skeletal muscle. PLoS One 5(12):e15239. [PubMed: 21179212]  [MGI Ref ID J:169000]

Viscomi C; Bottani E; Civiletto G; Cerutti R; Moggio M; Fagiolari G; Schon EA; Lamperti C; Zeviani M. 2011. In vivo correction of COX deficiency by activation of the AMPK/PGC-1alpha axis. Cell Metab 14(1):80-90. [PubMed: 21723506]  [MGI Ref ID J:176080]

Wang X; Pickrell AM; Zimmers TA; Moraes CT. 2012. Increase in muscle mitochondrial biogenesis does not prevent muscle loss but increased tumor size in a mouse model of acute cancer-induced cachexia. PLoS One 7(3):e33426. [PubMed: 22428048]  [MGI Ref ID J:187052]

Wenz T; Rossi SG; Rotundo RL; Spiegelman BM; Moraes CT. 2009. Increased muscle PGC-1alpha expression protects from sarcopenia and metabolic disease during aging. Proc Natl Acad Sci U S A 106(48):20405-10. [PubMed: 19918075]  [MGI Ref ID J:155572]

Wu J; Ruas JL; Estall JL; Rasbach KA; Choi JH; Ye L; Bostrom P; Tyra HM; Crawford RW; Campbell KP; Rutkowski DT; Kaufman RJ; Spiegelman BM. 2011. The unfolded protein response mediates adaptation to exercise in skeletal muscle through a PGC-1alpha/ATF6alpha complex. Cell Metab 13(2):160-9. [PubMed: 21284983]  [MGI Ref ID J:169564]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as hemizygotes. The Donating Investigator has not attempted to make the strain homozygous.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.8)