Strain Name: |
FVB/N-Tg(Ins2-IAPP)RHFSoel/J |
|---|---|
Stock Number: |
008232 |
Availability: | Under Development for Distribution Colony |
| To register your interest in this strain go to the Strain Interest Form. |
General Terms and Conditions |
| Genes & Alleles | IAPP; Ins2; Tg(Ins2-IAPP)RHFSoel; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Coisogenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Walter Soeller, Pfizer Inc Generation N10+ (22-JUL-08) Strain Description
Mice homozygous for the RIPHAT transgene are viable and fertile, with expression of human islet amyloid polypeptide (h-IAPP) under the regulatory control of the rat insulin II promoter. While h-IAPP RNA from the transgene is observed in pancreas, kidney, and stomach, h-IAPP protein is reported only in pancreas tissues. Hemizygous mice show no symptoms of spontaneous disease. Homozygous males spontaneously develop diabetes mellitus due to beta-cell death, associated with impaired insulin secretion (hypoinsulinemia), hyperglycemia, and abnormal intracellular aggregates of h-IAPP (the donating investigator reports that extracellular aggregates are not found on this strain background). Homozygous male onset is between 4-8 weeks of age with death around 16 weeks of age. Homozygous females exhibit a less severe phenotype. These RIPHAT transgenic mice may be useful in studying the beta-cell destruction and islet amyloid deposition associated with non-insulin-dependent diabetes mellitus (NIDDM) or Type II diabetes, as well as beta-cell apoptosis and characteristics of the endoplasmic reticulum (ER) stress pathway.Strain Development
The 2395 bp RIPHAT transgene was designed with (from 5' to 3') the rat insulin II promoter/5' untranslated region, a PCR-generated 270 bp cDNA encompassing the human islet amyloid polypeptide (h-IAPP) coding sequence, 728 bp from the human albumin gene introns 1, and the 525 bp polyadenylylation site/RNA termination region from the human glyceraldehyde-3-phosphate gene (GAPDH). This transgene was microinjected into the pronuclei of FVB/N fertilized oocytes. Mice from the founder line with the highest expression levels in the pancreas (founder line RHF) were established. These mice were maintained by breeding transgenic mice to one another and to FVB/N inbred mice for many generations prior to sending to The Jackson Laboratory. The donating investigator reports that high resolution mapping of the transgene in prometaphase spreads of endoreduplicated cells indicates the transgene insertion at a single location on chromosome 15 (15C); copy number is not known.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(Ins2-IAPP)RHFSoel | ||
|---|---|---|---|
| Allele Name | transgene insertion RHF, Walter C Soeller | ||
| Common Name(s) | RIPHAT; RIPHAT line RHF; Tg(IAPP)6Jdm; | ||
| Mutation Made By | Walter Soeller, Pfizer Inc | ||
| Strain of Origin | FVB/N | ||
| Expressed Gene | IAPP, islet amyloid polypeptide, human | ||
| Promoter | Ins2, insulin 2, rat | ||
| Molecular Note | The transgene consisted of cDNA encompassing the Human IAPP coding sequence under the regulation of the rat insulin II promoter (Ins2). [MGI Ref ID J:73886] | ||
Control Information
| Control | ||
|---|---|---|
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tg(Ins2-IAPP)RHFSoel
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, homozygous mice may be bred. Homozygous males exhibit onset of diabetes mellitus between 4-8 weeks of age with death around 16 weeks of age. Homozygous females exhibit a less severe phenotype. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Ins2
View Strains carrying other alleles of Ins2 (42 strains)
Additional Web Information
Congenic Nomenclature
Research Applications
This mouse can be used to support research in many areas including:
Apoptosis Research
Endogenous Regulators
Diabetes and Obesity Research
Hyperglycemia
Hypoinsulinemia
Insulin Resistance
Type 2 Diabetes (NIDDM)
Mouse/Human Gene Homologs
diabetes mellitus, insulin-resistant
Research Tools
Apoptosis Research
Diabetes and Obesity Research
References
Selected Reference(s)
Additional ReferencesCouce M; Kane LA; O'Brien TD; Charlesworth J; Soeller W; McNeish J; Kreutter D; Roche P; Butler PC. 1996. Treatment with growth hormone and dexamethasone in mice transgenic for human islet amyloid polypeptide causes islet amyloidosis and beta-cell dysfunction. Diabetes 45(8):1094-101. [PubMed: 8690157] [MGI Ref ID J:73886]
Janson J; Soeller WC; Roche PC; Nelson RT; Torchia AJ; Kreutter DK; Butler PC. 1996. Spontaneous diabetes mellitus in transgenic mice expressing human islet amyloid polypeptide. Proc Natl Acad Sci U S A 93(14):7283-8. [PubMed: 8692984] [MGI Ref ID J:47215]
Price and Supply Information
| Strain Name: | FVB/N-Tg(Ins2-IAPP)RHFSoel/J |
| Stock Number: | 008232 |
This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date: 29-SEP-08
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
View All Strains Under Development
Supply Details
| Standard Supply | Under Development for Distribution Colony |
|---|---|
| Supply Notes |
This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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