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Genes & Alleles

Nrgn;   Nrgntm1Kph;

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Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Heterozygote x Homozygote         (Female x Male) Species laboratory mouse Donating Investigator Kuo-Ping Huang,   NICHD, NIH Generation N11+ (04-SEP-08)

Strain Description
Homozygous neurogranin-deficient (Ng-/-) mice are viable and fertile (although the donating investigator reports that homozygous females do not nurse their pups as well as wildtype or heterozygous mothers). Homozygotes have no mRNA or protein from the targeted gene observed in brain tissues. Expression of lacZ is observed in a manner consistent with the endogenous gene. Ng-/- mice exhibit impaired spatial learning, altered hippocampal short- and long-term plasticity (including long-term potentiation induction), and decreased activated CaMKII. Heterozygotes show similar, yet milder, effects. These neurogranin- (Ng or RC3)-mutant mice may be useful for neurological studies involving memory and learning, neuronal signaling pathways (including calmodulin, alpha-CaMKII, protein kinase A, protein kinase C, MAPK, and CREB), attention deficit-hyperactivity disorder (ADHD), and schizophrenia.

Strain Development
A targeting vector was designed to replace the first five amino acids in exon 1 and the adjoining 98 bp of the first intron of the targeted gene with the lacZ and neomycin resistance genes. This construct was electroporated into 129/Sv-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and chimeric males were bred with C57BL/6 females to generate mutant mice. Next, mutant mice were backcrossed to C57BL/6J for at least 10 generations prior to arrival at The Jackson Laboratory.

Mammalian Phenotype Terms assigned by genotype The following phenotype information may relate to a genetic background differing from this JAX® Mice strain. Nrgntm1Kph/Nrgn+         involves: 129/Sv * C57BL/6 behavior/neurological phenotype abnormal contextual conditioning (MGI Ref ID J:65166) mice exhibit impaired long term memory in contextual fear conditioning test abnormal spatial learning (MGI Ref ID J:65166) spatial learning impairment in Morris water maze test although not as severely as homozygote Nrgntm1Kph/Nrgntm1Kph         involves: 129/Sv * C57BL/6 nervous system phenotype abnormal long term potentiation (MGI Ref ID J:65166) mice exhibit impaired long term potentiation (LTP) in the CA1 region and altered induction of LTP impaired synaptic plasticity (MGI Ref ID J:65166) mice exhibit impaired short term plasticity and altered induction behavior/neurological phenotype abnormal spatial learning (MGI Ref ID J:65166) spatial learning impairment in Morris water maze test

Gene & Allele Details

Allele Symbol		Nrgntm1Kph
Allele Name		targeted mutation 1, Kuo-Ping Huang
Common Name(s)		Ng-;
Mutation Made By		Kuo-Ping Huang,   NICHD, NIH
Strain of Origin		129/Sv
Site of Expression		lacZ is observed in a manner consistent with the endogenous gene
Gene Symbol and Name		Nrgn, neurogranin
Chromosome		9
Gene Common Name(s)		0710001B06Rik; AI838505; NG; NG/RC3; Pss1; R75334; RC3; RIKEN cDNA 0710001B06 gene; expressed sequence AI838505; expressed sequence R75334; hng; postsynaptic spine 1; protein kinase C substrate;
Molecular Note		Insertion of a LacZ gene in frame into the first coding exon, followed by a neomycin cassette. No NRGN immunoreactivity was detected in brain sections derived from homozygous mutant mice. X-gal staining in homozygous mutant mice corresponded with the NRGN positive staining in wild type mice. [MGI Ref ID J:65166]

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Genotyping Protocols

Nrgn^tm1Kph

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, heterozygous females are bred with homozygous males. The donating investigator reports that homozygous females do not nurse their pups as well as wildtype or heterozygous mothers.

Additional Web Information

Congenic Nomenclature

Research Applications

This mouse can be used to support research in many areas including:

Cell Biology Research
Signal Transduction

Neurobiology Research
lacZ expression in neural tissue
Behavioral and Learning Defects

Research Tools
lacZ Expression
Genetics Research (Tissue/Cell Markers: neurons)
Neurobiology Research (cell marker)

References

Selected Reference(s)

Pak JH; Huang FL; Li J; Balschun D; Reymann KG; Chiang C; Westphal H; Huang KP. 2000. Involvement of neurogranin in the modulation of calcium/calmodulin-dependent protein kinase II, synaptic plasticity, and spatial learning: A study with knockout mice Proc Natl Acad Sci U S A 97(21):11232-7. [PubMed: 11016969]  [MGI Ref ID J:65166]

Additional References

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Price and Supply Information

Strain Name:	B6.129-Nrgntm1Kph/J
Stock Number:	008233

 

This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.

Estimated Available for Sale Date:

Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.

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Supply Details

Standard Supply	Under Development for Distribution Colony
Supply Notes	This strain is included in the Induced Mutant Resource Colony collection.
Licensing	See General Terms and Conditions below for Licensing and Use Restrictions
Control Information	View Control Information in Strain Details.

General Terms and Conditions

View JAX^® Mice & Services Conditions of Use.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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