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| These NG2DsRedBAC transgenic mice express an optimized red fluorescent protein variant (DsRed.T1) under the control of the mouse NG2 (Cspg4) promoter/enhancer and may be useful for fluorescent labeling of NG2 cells (oligodendrocyte progenitor cells) in the central nervous system and NG2-expressing cells in other organs, as well as for isolating NG2 cell populations via FACS. | |||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
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Description
Strain Information
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered Mutant Mice. Mating System Noncarrier x Hemizygote (Female x Male) Species laboratory mouse Generation F?+ (06-NOV-08) Donating Investigator Akiko Nishiyama, University of Connecticut Description
Mice hemizygous for the NG2DsRedBAC transgene are viable and fertile, expressing an optimized red fluorescent protein variant (DsRed.T1) under the control of the mouse NG2 (Cspg4) promoter/enhancer. DsRed.T1 fluorescence is detected in NG2 cells (oligodendrocyte progenitor cells) throughout the postnatal central nervous system, including the gray and white matter of the brain, cerebellum and spinal cord, and vascular mural cells; but not in mature oligodendrocytes, astrocytes, resting microglia, or neurons. DsRed.T1 fluorescence intensity is highest in cell bodies and can also be detected in distal processes, suggesting that tetrameric DsRed.T1 remains soluble and is not toxic to cells. In addition, DsRed.T1 fluorescence may be readily detected without using anti-DsRed antibodies and is suitable for identifying NG2 cells in live slices or for purifying NG2 cells via FACS. These NG2DsRedBAC transgenic mice may be useful for fluorescent labeling of NG2 cells (oligodendrocyte progenitor cells) in the central nervous system and NG2-expressing cells in other organs.Development
The 208 kb C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-309G21, containing the entire NG2 (Cspg4) gene (and 60 kbp of 5' and 114 kbp of 3' flanking sequences), was modified by inserting an optimized red fluorescent protein variant (DsRed.T1) coding sequence followed by a rabbit beta-globin polyA signal into the first exon of the NG2 gene. The NG2 translation initiation ATG was changed to AAG to prevent translation from the BAC NG2 gene and to allow translation from the first ATG in the DsRed.T1 sequence. DNA from a correctly modified BAC clone was used as the source for the NG2DsRedBAC transgene, which was linearized and microinjected into the pronucleus of fertilized oocytes from B6SJL/F1 mice. Founder mice with the highest levels of DsRed.T1 expression were selected and used to establish NG2DsRedBAC transgenic mice. NG2DsRedBAC transgenic mice were maintained on a mixed C57BL/6, SJL, and FVB genetic background prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (169 strains)
008533 B6;FVB-Tg(Cspg4-cre)1Akik/J
006051 B6.Cg-Tg(CAG-DsRed*MST)1Nagy/J 006872 B6.Cg-Tg(Ins1-DsRed*T4)32Hara/J 008605 B6;C3-Tg(CAG-DsRed,-EGFP)5Gae/J 005328 NOD/ShiLt-Tg(Cd4-DsRed)4Lt/J 005438 STOCK Tg(CAG-Bgeo,-DsRed*MST)1Nagy/J 005441 STOCK Tg(CAG-DsRed*MST)1Nagy/J 006866 STOCK Tg(Ins1-DsRed*T4)32Hara/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(Cspg4-DsRed.T1)1Akik/0
involves: C57BL/6 * SJL
- no phenotypic analysis
- *normal* no phenotypic analysis (MGI Ref ID J:129201)
This mouse can be used to support research in many areas including:
DsRed relatedNeurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Developmental Biology Research (transplantation marker for embryonic and adult tissue)
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers: glial cells)
Genetics Research (Tissue/Cell Markers: glial cells, oligodendrocytes, Schwann cells)
Genetics Research (Tissue/Cell Markers: neurons)
Genetics Research (Tissue/Cell Markers: transplantation marker for embryonic and adult tissue)
Neurobiology Research (cell marker)
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Cspg4-DsRed.T1)1Akik | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Akiko Nishiyama | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | NG2DsRedBAC; | ||
| Mutation Made By | Akiko Nishiyama, University of Connecticut | ||
| Strain of Origin | (C57BL/6 x SJL)F1 | ||
| Site of Expression | DsRed.T1 fluorescence is detected in NG2 cells (oligodendrocyte progenitor cells) throughout the postnatal central nervous system, including the gray and white matter of the brain, cerebellum and spinal cord, and vascular mural cells; but not in mature oligodendrocytes, astrocytes, resting microglia, or neurons. DsRed.T1 fluorescence intensity is highest in cell bodies and can also be detected in distal processes. | ||
| Expressed Gene | DsRed, red fluorescent protein, | ||
| Promoter | Cspg4, chondroitin sulfate proteoglycan 4, mouse, laboratory | ||
| Molecular Note | The transgene construct consisted of the 208 kb C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-309G21, containing the entire NG2 (Cspg4) gene (and 60 kbp of 5' and 114 kbp of 3' flanking sequences), which was modified by inserting an optimizedred fluorescent protein variant (DsRed.T1) coding sequence followed by a rabbit beta-globin polyA signal into the first exon of the NG2 gene. The NG2 translation initiation ATG was changed to AAG to prevent translation from the BAC NG2 gene and to allow translation from the first ATG in the DsRed.T1 sequence. [MGI Ref ID J:129201] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Cspg4-DsRed.T1)1Akik, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Zhu X; Bergles DE; Nishiyama A. 2008. NG2 cells generate both oligodendrocytes and gray matter astrocytes. Development 135(1):145-57. [PubMed: 18045844] [MGI Ref ID J:129201]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, transgenic carrier mice may be bred together or to wildtype siblings. The donating investigator reports that presumed homozygotes seem to have smaller litter sizes. Mating System Noncarrier x Hemizygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date: 08-DEC-08
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
View All Strains Under Development and On Hold
Supply Details
| Standard Supply | Under Development for Distribution Colony |
|---|---|
| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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