Strain Name:

129;B6-Tff3tm1Dkpy/J

Stock Number:

008243

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
These intestinal trefoil factor (ITF or Tff3) mutant mice may be useful in studying gastrointestinal tract injury (including inflammatory bowel diseases), maintenance of the mucosal barrier, migration and turnover of the intestinal epithelium, and therapies for colon cancer.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names 129-Tff3tm1Dkpy/J    (Changed: 29-JUL-09 )
Type Mutant Stock; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
GenerationN1pN1
Generation Definitions
 
Donating Investigator Daniel K Podolsky,   Massachusetts General Hospital

Description
Mice homozygous for this intestinal trefoil factor (ITF or Tff3) mutant allele are viable and fertile with no RNA or protein expression of the targeted gene in the gastrointestinal tract. Homozygous mice exhibit impaired physiological migration of intestinal epithelium to the mucosal surface, have impaired mucosal healing and increased susceptibility to dextran sulfate sodium (DSS)- induced colitis, and are more susceptible to chemotherapy and radiation-induced mucositis. These intestinal trefoil factor (ITF or Tff3) mutant mice may be useful in studying gastrointestinal tract injury (including inflammatory bowel diseases), maintenance of the mucosal barrier, migration and turnover of the intestinal epithelium, and therapies for colon cancer.

Development
A targeting vector was designed to replace exon 2 of the targeted gene with a neomycin resistance gene cassette. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and chimeric mice were identified. These mutant mice were maintained on a "129" genetic background prior to arrival at The Jackson Laboratory. Upon arrival, mutant mice were bred with 129S1/SvImJ mice for at least one generation to establish the colony.

A 27 SNP (single nucleotide polymorphism) panel analysis performed by The Jackson Laboratory on the rederived mice revealed six segregating markers (each on a different chromosome). All six loci suggest this strain is on a mixed 129;C57BL/6 genetic background (July 2009).

Control Information

  Control
   002448 129S1/SvImJ (approximate)
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Tff3tm1Dkpy/Tff3tm1Dkpy

        involves: 129S4/SvJae
  • digestive/alimentary phenotype
  • abnormal intestinal epithelium morphology
    • impaired physiological migration of epithelium to mucosal surface   (MGI Ref ID J:35882)
  • increased susceptibility to induced colitis
    • increased development of colitis (colon ulceration, abscesses, and hemorrhage) after dextran sulfate sodium (DSS) administration   (MGI Ref ID J:35882)
  • immune system phenotype
  • increased susceptibility to induced colitis
    • increased development of colitis (colon ulceration, abscesses, and hemorrhage) after dextran sulfate sodium (DSS) administration   (MGI Ref ID J:35882)
  • homeostasis/metabolism phenotype
  • increased susceptibility to injury
    • increased development of colitis (colon ulceration, abscesses, and hemorrhage) after dextran sulfate sodium (DSS) administration   (MGI Ref ID J:35882)
    • increased development of bloody diarrhea and death after (DSS) administration   (MGI Ref ID J:35882)
    • increased weight loss after (DSS) administration   (MGI Ref ID J:35882)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Endocrine Deficiency Research
Gastrointestinal Defects

Immunology, Inflammation and Autoimmunity Research
Immunodeficiency
      Inflammatory bowel disease
Inflammation
      Inflammatory bowel disease

Internal/Organ Research
Gastrointestinal Defects
      colitis
Wound Healing
      delayed/impaired

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tff3tm1Dkpy
Allele Name targeted mutation 1, Daniel K Podolsky
Allele Type Targeted (knock-out)
Common Name(s) Itf-;
Mutation Made By Daniel Podolsky,   Massachusetts General Hospital
Strain of Origin129S4/SvJae
ES Cell Line NameJ1
ES Cell Line Strain129S4/SvJae
Gene Symbol and Name Tff3, trefoil factor 3, intestinal
Chromosome 17
Gene Common Name(s) ITF; P1B; TFI; mITF;
Molecular Note Exon 2 of this gene was replaced with a neo cassette via homologous recombination resulting in the deletion of the trefoil domain. Absence of gene expression in the gastrointestinal tract of homozygous mutant animals was confirmed by Northern blot analysis and immunohistochemistry. [MGI Ref ID J:35882]

Genotyping

Genotyping Information

Genotyping Protocols

Tff3tm1Dkpy, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Mashimo H; Wu DC; Podolsky DK; Fishman MC. 1996. Impaired defense of intestinal mucosa in mice lacking intestinal trefoil factor [see comments] Science 274(5285):262-5. [PubMed: 8824194]  [MGI Ref ID J:35882]

Additional References

Tff3tm1Dkpy related

Beck PL; Ihara E; Hirota SA; MacDonald JA; Meng D; Nanthakumar NN; Podolsky DK; Xavier RJ. 2010. Exploring the interplay of barrier function and leukocyte recruitment in intestinal inflammation by targeting fucosyltransferase VII and trefoil factor 3. Am J Physiol Gastrointest Liver Physiol 299(1):G43-53. [PubMed: 20299601]  [MGI Ref ID J:162267]

Beck PL; Wong JF; Li Y; Swaminathan S; Xavier RJ; Devaney KL; Podolsky DK. 2004. Chemotherapy- and radiotherapy-induced intestinal damage is regulated by intestinal trefoil factor. Gastroenterology 126(3):796-808. [PubMed: 14988834]  [MGI Ref ID J:107673]

Paulsen FP; Woon CW; Varoga D; Jansen A; Garreis F; Jager K; Amm M; Podolsky DK; Steven P; Barker NP; Sel S. 2008. Intestinal trefoil factor/TFF3 promotes re-epithelialization of corneal wounds. J Biol Chem 283(19):13418-27. [PubMed: 18326859]  [MGI Ref ID J:137338]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice may be bred together.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2450.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3185.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   002448 129S1/SvImJ (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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