Description

Strain Information

Type Congenic; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Noncarrier x Hemizygote         (Female x Male)   23-OCT-08 Species laboratory mouse Generation N21+F2 (14-JAN-09)   Donating Investigator Todd Schell,   Penn State Univ. College of Medicine

Description
Hemizygous T1-147 transgenic mice (harboring the E1-T147 transgene) are viable and fertile, with high-level expression of an N-terminal, truncated (1-147 amino acid), and mutant (does not express the small t antigen) form of simian virus 40 large T antigen (TAg) directed to pancreatic acinar cells by the rat elastase 1 (Ela1 or E1) upstream control region. Hemizygous T1-147 transgenic mice exhibit pancreatic dysplasia in the embryonic pancreas, with cancer progression after birth to primary pancreatic acinar cell tumor formation (100% penetrance). These mice have microadenomas and acinar cell carcinomas present as early as 10 weeks of age, and become moribund or show clear evidence of abdominal distention around 20-30 weeks of age. T1-147 transgenic mice with large tumors develop metastatic disease to the liver and mesenteric lymph nodes. The donating investigator reports that the cancer phenotype of these T1-147 transgenic mice is identical to that of their similar T1-127 transgenic mice. These T1-147 transgenic mice may be useful in studying pancreatic acinar cell cancer and metastasis.

Development
The E1-T147 transgene was designed with a 4.3 kb rat elastase 1 (Ela1 or E1) gene upstream control region, LTR stabilizer, and SV40 late 16S splice site all upstream of the simian virus 40 large T antigen (TAg) coding sequence for amino acids 1-147 (with stop codon introduced at amino acid 148) and polyA sequence. This transgene was microinjected into pronuclei of B6D2F1/J x C57BL/6 embryos, and the resulting transgenic offspring were bred to C57BL/6J mice. The T1-147 transgenic mice (founder line 289) mice were established, and subsequently backcrossed to C57BL/6 inbred mice for at least 20 generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred with C57BL/6J (Stock No. 000664) to establish the colony.

Control Information

	Control
	Noncarrier
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Cela1

008861

STOCK Tg(Ela1-Cre/ESR1)1Stof/J

View Strains carrying other alleles of Cela1     (1 strain)

Strains carrying other alleles of TAg

008215	(C57BL/6-Tg(TRAMP)8247Ng/J X FVB/NJ)F1/J
003382	B10.D2-Tg(C3-1-TAg)cJeg/J
003380	B6.FVB-Tg(C3-1-TAg)cJeg/J
003135	C57BL/6-Tg(TRAMP)8247Ng/J
003445	C57BL/6J-Tg(Amy1TAg)354Knw/J
003446	C57BL/6J-Tg(Amy1TAg)501Knw/J
003477	C57BL/6J-Tg(SV)419Bri/J
003476	C57BL/6J-Tg(SV)427Bri/J
002233	C57BL/6J-Tg(SV)7Bri/J
003189	C57BL/6J-Tg(WAPTAg)3Knw/J
003188	C57BL/6J-Tg(WapTAg)1Knw
005564	FVB(Cg)-Tg(Ins2-CALM1)26Ove Tg(Cryaa-TAg)1Ove/PneJ
003381	FVB-Tg(C3-1-TAg)cJeg/J
002380	NOD.Cg-Tg(Ins2-TAg)1Lt Prkdcscid/DvsJ
002033	NOD/ShiLt-Tg(RipTAg)1Lt/J

View Strains carrying other alleles of TAg     (15 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Tg(Ela1-TAg*)289Mjt/0

        involves: C57BL/6 * DBA/2

• life span-post-weaning/aging
• premature death (MGI Ref ID J:78026)
  • mice become moribund around 20-30 weeks of age
• growth/size phenotype
• distended abdomen (MGI Ref ID J:78026)
  • many mice show clear abdominal distention resulting from tumor growth around 20-30 weeks of age
• tumorigenesis
• pancreas tumor (MGI Ref ID J:78026)
  • mice develop pancreatic tumors between 20.5 and 30.5 weeks of age; tumor histological features are indistinguishable from tumors in Tg(Ela1-Tag*)79Mjt animals
• pancreas adenoma (MGI Ref ID J:78026)
  • transgenic mice show acinar microadenomas as early as 10 weeks of age
• pancreatic acinar cell carcinoma (MGI Ref ID J:78026)
  • acinar cell carcinomas are apparent as early as 10 weeks of age
• digestive/alimentary phenotype
• abnormal pancreas morphology (MGI Ref ID J:78026)
  • embryonic pancreas shows dysplasia in transgenic mice
  • after birth, pancreatic dysplasia progresses to primary pancreatic acinar cell tumors with 100% penetrance
• endocrine/exocrine gland phenotype
• abnormal pancreas morphology (MGI Ref ID J:78026)
  • embryonic pancreas shows dysplasia in transgenic mice
  • after birth, pancreatic dysplasia progresses to primary pancreatic acinar cell tumors with 100% penetrance

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cancer Research
Increased Tumor Incidence
      Adenomas
      Hepatomas
      Other Tissues/Organs
Other
      tumor metastasis

Endocrine Deficiency Research
Pancreas Defects

Research Tools
Cancer Research

TAg related

Apoptosis Research
Extracellular Modulators

Genes & Alleles

Gene & Allele Information

Allele Symbol		Tg(Ela1-TAg*)289Mjt
Allele Name		transgene insertion 289, Mary J Tevethia
Allele Type		Transgenic (random, expressed)
Common Name(s)		T1-147;
Mutation Made By		Mary Tevethia,   Penn State Univ. College of Medicine
Strain of Origin		(B6D2F1/J x C57BL/6)
Expressed Gene		TAg, SV40 large T-antigen, SV40
		Simian virus 40 T antigen (SV40Tag) is a multifunctional regulatory protein that stimulates gene transcription and forms complexes with cell cycle-regulatory proteins such as Trp53 and Rb1 that are implicated in human breast cancer.
Promoter		Cela1, chymotrypsin-like elastase family, member 1, rat
Molecular Note		The E1-T147 transgene was designed with a 4.3 kb rat elastase 1 (Ela1 or E1) gene upstream control region, LTR stabilizer, and SV40 late 16S splice site all upstream of the simian virus 40 large T antigen (TAg) coding sequence for amino acids 1-147 (withstop codon introduced at amino acid 148) and polyA sequence. The T1-147 transgenic mice (founder line 289) mice were established. [MGI Ref ID J:78026]

Genotyping

Genotyping Information

Genotyping Protocols

Tg(Ela1-TAg*)289Mjt, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Tevethia MJ; Bonneau RH; Griffith JW; Mylin L. 1997. A simian virus 40 large T-antigen segment containing amino acids 1 to 127 and expressed under the control of the rat elastase-1 promoter produces pancreatic acinar carcinomas in transgenic mice. J Virol 71(11):8157-66. [PubMed: 9343166]  [MGI Ref ID J:78026]

Additional References

Tg(Ela1-TAg*)289Mjt related

Lindsay TH; Jonas BM; Sevcik MA; Kubota K; Halvorson KG; Ghilardi JR; Kuskowski MA; Stelow EB; Mukherjee P; Gendler SJ; Wong GY; Mantyh PW. 2005. Pancreatic cancer pain and its correlation with changes in tumor vasculature, macrophage infiltration, neuronal innervation, body weight and disease progression. Pain 119(1-3):233-46. [PubMed: 16298491]  [MGI Ref ID J:106752]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX11

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, hemizygous mice may be bred with noncarrier littermates or with C57BL/6J inbred mice. The donating investigator breeds hemizygous males with noncarrier females, and reports decreased litter sizes may result from breeding to homozygosity.
Mating System	Noncarrier x Hemizygote         (Female x Male)   23-OCT-08
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement.

Supply Notes

Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Noncarrier
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Terms of Use

Terms of Use

General Terms and Conditions

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Contact information

General inquiries

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phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

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