Strain Name:

B6.Cg-Tg(Ela1-TAg*)289Mjt/J

Stock Number:

008247

Availability:

Under Development for Distribution Colony

Use Restrictions Apply, see Terms of Use
To register your interest in this strain go to the Strain Interest Form.
These T1-147 transgenic mice (harboring the E1-T147 transgene) develop metastatic pancreatic acinar cell cancer as a result of expression of the 1-147 amino acid fragment of simian virus 40 large T antigen (TAg) directed to pancreatic acinar cells by the rat elastase 1 (Ela1 or E1) upstream control region.

Description

Strain Information

Type Congenic; Transgenic;
Additional information on Genetically Engineered Mutant Mice.
Mating SystemNoncarrier x Hemizygote         (Female x Male)
Specieslaboratory mouse
GenerationN21+ (06-OCT-08)
 
Donating Investigator Todd Schell,   Penn State Univ. College of Medicine

Description
Hemizygous T1-147 transgenic mice (harboring the E1-T147 transgene) are viable and fertile, with high-level expression of an N-terminal, truncated (1-147 amino acid), and mutant (does not express the small t antigen) form of simian virus 40 large T antigen (TAg) directed to pancreatic acinar cells by the rat elastase 1 (Ela1 or E1) upstream control region. Hemizygous T1-147 transgenic mice exhibit pancreatic dysplasia in the embryonic pancreas, with cancer progression after birth to primary pancreatic acinar cell tumor formation (100% penetrance). These mice have microadenomas and acinar cell carcinomas present as early as 10 weeks of age, and become moribund or show clear evidence of abdominal distention around 20-30 weeks of age. T1-147 transgenic mice with large tumors develop metastatic disease to the liver and mesenteric lymph nodes. The donating investigator reports that the cancer phenotype of these T1-147 transgenic mice is identical to that of their similar T1-127 transgenic mice. These T1-147 transgenic mice may be useful in studying pancreatic acinar cell cancer and metastasis.

Development
The E1-T147 transgene was designed with a 4.3 kb rat elastase 1 (Ela1 or E1) gene upstream control region, LTR stabilizer, and SV40 late 16S splice site all upstream of the simian virus 40 large T antigen (TAg) coding sequence for amino acids 1-147 (with stop codon introduced at amino acid 148) and polyA sequence. This transgene was microinjected into pronuclei of B6D2F1/J x C57BL/6 embryos, and the resulting transgenic offspring were bred to C57BL/6J mice. The T1-147 transgenic mice (founder line 289) mice were established, and subsequently backcrossed to C57BL/6 inbred mice for at least 20 generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred with C57BL/6J (Stock No. 000664) to establish the colony.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of TAg     (15 strains)

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms
      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Tg(Ela1-TAg*)289Mjt/0

        involves: C57BL/6 * DBA/2
  • life span-post-weaning/aging
  • premature death (MGI Ref ID J:78026)
    • mice become moribund around 20-30 weeks of age
  • growth/size phenotype
  • distended abdomen (MGI Ref ID J:78026)
    • many mice show clear abdominal distention resulting from tumor growth around 20-30 weeks of age
  • tumorigenesis
  • increased tumor incidence (MGI Ref ID J:78026)
    • mice develop pancreatic tumors between 20.5 and 30.5 weeks of age; tumor histological features are indistinguishable from tumors in Tg(Ela1-Tag*)79Mjt animals
    • adenoma (MGI Ref ID J:78026)
      • transgenic mice show acinar microadenomas as early as 10 weeks of age
    • carcinoma (MGI Ref ID J:78026)
      • acinar cell carcinomas are apparent as early as 10 weeks of age
  • digestive/alimentary phenotype
  • abnormal pancreas morphology (MGI Ref ID J:78026)
    • embryonic pancreas shows dysplasia in transgenic mice
    • after birth, pancreatic dysplasia progresses to primary pancreatic acinar cell tumors with 100% penetrance
  • endocrine/exocrine gland phenotype
  • abnormal pancreas morphology (MGI Ref ID J:78026)
    • embryonic pancreas shows dysplasia in transgenic mice
    • after birth, pancreatic dysplasia progresses to primary pancreatic acinar cell tumors with 100% penetrance
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cancer Research
Increased Tumor Incidence (Adenomas)
Increased Tumor Incidence (Hepatomas)
Increased Tumor Incidence (Other Tissues/Organs)
Other (tumor metastasis)

Endocrine Deficiency Research
Pancreas Defects

Research Tools
Cancer Research

TAg related

Apoptosis Research
Extracellular Modulators

Genes & Alleles

Gene & Allele Information

Allele Symbol Tg(Ela1-TAg*)289Mjt
Allele Name transgene insertion 289, Mary J Tevethia
Allele Type Transgenic (random, expressed)
Common Name(s) T1-147;
Mutation Made By Mary Tevethia,   Penn State Univ. College of Medicine
Strain of Origin(B6D2F1/J x C57BL/6)
Expressed Gene TAg, SV40 large T-antigen, SV40
Simian virus 40 T antigen (SV40Tag) is a multifunctional regulatory protein that stimulates gene transcription and forms complexes with cell cycle-regulatory proteins such as Trp53 and Rb1 that are implicated in human breast cancer.
Promoter Ela1, elastase 1, pancreatic, rat
Molecular Note The E1-T147 transgene was designed with a 4.3 kb rat elastase 1 (Ela1 or E1) gene upstream control region, LTR stabilizer, and SV40 late 16S splice site all upstream of the simian virus 40 large T antigen (TAg) coding sequence for amino acids 1-147 (withstop codon introduced at amino acid 148) and polyA sequence. The T1-147 transgenic mice (founder line 289) mice were established. [MGI Ref ID J:78026]

Genotyping

Genotyping Information

Genotyping Protocols

Tg(Ela1-TAg*)289Mjt, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Tevethia MJ; Bonneau RH; Griffith JW; Mylin L. 1997. A simian virus 40 large T-antigen segment containing amino acids 1 to 127 and expressed under the control of the rat elastase-1 promoter produces pancreatic acinar carcinomas in transgenic mice. J Virol 71(11):8157-66. [PubMed: 9343166]  [MGI Ref ID J:78026]

Additional References

Tg(Ela1-TAg*)289Mjt related

Lindsay TH; Jonas BM; Sevcik MA; Kubota K; Halvorson KG; Ghilardi JR; Kuskowski MA; Stelow EB; Mukherjee P; Gendler SJ; Wong GY; Mantyh PW. 2005. Pancreatic cancer pain and its correlation with changes in tumor vasculature, macrophage infiltration, neuronal innervation, body weight and disease progression. Pain 119(1-3):233-46. [PubMed: 16298491]  [MGI Ref ID J:106752]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, hemizygous mice may be bred with noncarrier littermates or with C57BL/6J inbred mice. The donating investigator breeds hemizygous males with noncarrier females, and reports decreased litter sizes may result from breeding to homozygosity.
Mating SystemNoncarrier x Hemizygote         (Female x Male)
Diet Information LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

 

This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.

Estimated Available for Sale Date: 22-DEC-08

Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.

View All Strains Under Development and On Hold

Supply Details

Standard SupplyUnder Development for Distribution Colony
Supply Notes

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

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