Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse   Donating Investigator Todd D. Schell,   Penn State Univ. College of Medicine

Description
Hemizygous T1-147 transgenic mice (harboring the E1-T147 transgene) are viable and fertile, with high-level expression of an N-terminal, truncated (1-147 amino acid), and mutant (does not express the small t antigen) form of simian virus 40 large T antigen (TAg) directed to pancreatic acinar cells by the rat elastase 1 (Ela1 or E1) upstream control region. Hemizygous T1-147 transgenic mice exhibit pancreatic dysplasia in the embryonic pancreas, with cancer progression after birth to primary pancreatic acinar cell tumor formation (100% penetrance). These mice have microadenomas and acinar cell carcinomas present as early as 10 weeks of age, and become moribund or show clear evidence of abdominal distention around 20-30 weeks of age. T1-147 transgenic mice with large tumors develop metastatic disease to the liver and mesenteric lymph nodes. The donating investigator reports that the cancer phenotype of these T1-147 transgenic mice is identical to that of their similar T1-127 transgenic mice. These T1-147 transgenic mice may be useful in studying pancreatic acinar cell cancer and metastasis.

Development
The E1-T147 transgene was designed with a 4.3 kb rat elastase 1 (Ela1 or E1) gene upstream control region, LTR stabilizer, and SV40 late 16S splice site all upstream of the simian virus 40 large T antigen (TAg) coding sequence for amino acids 1-147 (with stop codon introduced at amino acid 148) and polyA sequence. This transgene was microinjected into pronuclei of B6D2F1/J x C57BL/6 embryos, and the resulting transgenic offspring were bred to C57BL/6J mice. The T1-147 transgenic mice (founder line 289) mice were established, and subsequently backcrossed to C57BL/6 inbred mice for at least 20 generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred with C57BL/6J (Stock No. 000664) to establish the colony.

Control Information

	Control
	Noncarrier
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Cela1

008861

STOCK Tg(Ela1-Cre/ERT2)1Stof/J

View Strains carrying other alleles of Cela1     (1 strain)

Strains carrying other alleles of TAg

008215	(C57BL/6-Tg(TRAMP)8247Ng/J x FVB/NJ)F1/J
017719	C3HeB/FeJ-Tg(tetO-TAg)1Efr/J
003135	C57BL/6-Tg(TRAMP)8247Ng/J
003445	C57BL/6J-Tg(Amy1TAg)354Knw/J
003446	C57BL/6J-Tg(Amy1TAg)501Knw/J
003477	C57BL/6J-Tg(SV)419Bri/J
003476	C57BL/6J-Tg(SV)427Bri/J
002233	C57BL/6J-Tg(SV)7Bri/J
003189	C57BL/6J-Tg(WAPTAg)3Knw/J
003188	C57BL/6J-Tg(WapTAg)1Knw/J
005564	FVB(Cg)-Tg(Ins2-CALM1)26Ove Tg(Cryaa-TAg)1Ove/PneJ
013591	FVB-Tg(C3-1-TAg)cJeg/JegJ
002380	NOD.Cg-Tg(Ins2-TAg)1Lt Prkdcscid/DvsJ
002033	NOD/ShiLt-Tg(Ins2-TAg)1Lt/J

View Strains carrying other alleles of TAg     (14 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Tg(Ela1-TAg*)289Mjt/0

        involves: C57BL/6 * DBA/2

• mortality/aging
• premature death
  • mice become moribund around 20-30 weeks of age   (MGI Ref ID J:78026)
• growth/size phenotype
• distended abdomen
  • many mice show clear abdominal distention resulting from tumor growth around 20-30 weeks of age   (MGI Ref ID J:78026)
• tumorigenesis
• increased pancreas tumor incidence
  • mice develop pancreatic tumors between 20.5 and 30.5 weeks of age; tumor histological features are indistinguishable from tumors in Tg(Ela1-Tag*)79Mjt animals   (MGI Ref ID J:78026)
• • increased pancreas adenoma incidence
    • transgenic mice show acinar microadenomas as early as 10 weeks of age   (MGI Ref ID J:78026)
  • increased pancreatic acinar cell carcinoma incidence
    • acinar cell carcinomas are apparent as early as 10 weeks of age   (MGI Ref ID J:78026)
• endocrine/exocrine gland phenotype
• abnormal pancreas morphology
  • embryonic pancreas shows dysplasia in transgenic mice   (MGI Ref ID J:78026)
  • after birth, pancreatic dysplasia progresses to primary pancreatic acinar cell tumors with 100% penetrance   (MGI Ref ID J:78026)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cancer Research
Increased Tumor Incidence
      Adenomas
      Hepatomas
      Other Tissues/Organs
Other
      tumor metastasis

Endocrine Deficiency Research
Pancreas Defects

Research Tools
Cancer Research

TAg related

Apoptosis Research
Extracellular Modulators

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Tg(Ela1-TAg*)289Mjt
Allele Name		transgene insertion 289, Mary J Tevethia
Allele Type		Transgenic (random, expressed)
Common Name(s)		T1-147;
Mutation Made By		Mary Tevethia,   Penn State Univ. College of Medicine
Strain of Origin		(B6D2F1/J x C57BL/6)
Expressed Gene		TAg, SV40 large T-antigen, SV40
		Simian virus 40 T antigen (SV40Tag) is a multifunctional regulatory protein that stimulates gene transcription and forms complexes with cell cycle-regulatory proteins such as Trp53 and Rb1 that are implicated in human breast cancer.
Promoter		Cela1, chymotrypsin-like elastase family, member 1, rat
Molecular Note		The E1-T147 transgene was designed with a 4.3 kb rat elastase 1 (Ela1 or E1) gene upstream control region, LTR stabilizer, and SV40 late 16S splice site all upstream of the simian virus 40 large T antigen (TAg) coding sequence for amino acids 1-147 (withstop codon introduced at amino acid 148) and polyA sequence. The T1-147 transgenic mice (founder line 289) mice were established. [MGI Ref ID J:78026]

Genotyping

Genotyping Information

Genotyping Protocols

Generic SV40, Fast MCA
Tg(Ela1-TAg*)289Mjt, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Tevethia MJ; Bonneau RH; Griffith JW; Mylin L. 1997. A simian virus 40 large T-antigen segment containing amino acids 1 to 127 and expressed under the control of the rat elastase-1 promoter produces pancreatic acinar carcinomas in transgenic mice. J Virol 71(11):8157-66. [PubMed: 9343166]  [MGI Ref ID J:78026]

Additional References

Tg(Ela1-TAg*)289Mjt related

Lindsay TH; Jonas BM; Sevcik MA; Kubota K; Halvorson KG; Ghilardi JR; Kuskowski MA; Stelow EB; Mukherjee P; Gendler SJ; Wong GY; Mantyh PW. 2005. Pancreatic cancer pain and its correlation with changes in tumor vasculature, macrophage infiltration, neuronal innervation, body weight and disease progression. Pain 119(1-3):233-46. [PubMed: 16298491]  [MGI Ref ID J:106752]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, hemizygous mice may be bred with noncarrier littermates or with C57BL/6J inbred mice. The donating investigator breeds hemizygous males with noncarrier females, and reports decreased litter sizes may result from breeding to homozygosity.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

General Supply Notes

• Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Noncarrier
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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