Strain Name: |
STOCK Tg(Ins2-rtTA)2Efr/J |
|---|---|
Stock Number: |
008250 |
Availability: | Under Development for Distribution Colony |
| To register your interest in this strain go to the Strain Interest Form. |
General Terms and Conditions |
| Strain Common Names | Ins-rtTA; RIP7-rtTA; RiprtTA; |
| Genes & Alleles | Ins2; rtTA; Tg(Ins2-rtTA)2Efr; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Douglas Melton, Harvard University Strain Description
These mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the rat insulin 2 promoter. When mated to a second transgenic strain carrying a gene under the regulatory control of a tetracycline-responsive promoter element (tetO), expression of the gene may be regulated by the tetracycline analog, doxycycline (dox); in the presence of dox, transcription of the target gene is induced in pancreatic beta cells where rtTA is expressed. Induction of rtTA activity was not detected in other tissues. This strain provides a Tet-On tool that allows the inducible expression of genes in pancreatic beta cells. The Donating Investigator reports that homozygotes are viable and fertile.Strain Development
A transgenic construct containing the reverse tetracycline-controlled transactivator (rtTA) gene, 9.5kb 5' regulatory sequence and first intron of the rat insulin 2 gene (RIP7) and polyadenylation site sequence was injected into fertilized (C57BL/6 X CBA)F2 mouse eggs. Founder line 2 with 10 copies of the transgene was established and backcrossed to C57BL/6 for at least 3 generations. The mice were then maintained on the outbred ICR background. The mice were then crosssed to C57BL/6J once at The Jackson Laboratory.
Gene & Allele Details
| Allele Symbol | Tg(Ins2-rtTA)2Efr | ||
|---|---|---|---|
| Allele Name | transgene insertion 2, Shimon Efrat | ||
| Common Name(s) | Ins-rtTA; RIP7-rtTA; RiprtTA; | ||
| Strain of Origin | (C57BL/6 x CBA)F2 | ||
| Site of Expression | pancreatic beta cells | ||
| Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli | ||
| The tetracycline repressor gene (Tetr), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). One mutant with a four amino acid residue change (rTetR) exhibited dependence on tetracycline for induction of the targeted gene and was used in the rtTA construct (Gossen et al, 1995). rTetr was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). | |||
| Promoter | Ins2, insulin 2, rat | ||
| Molecular Note | A transgenic construct containing 9.5kb 5' regulatory sequence and first intron of the rat insulin 2 gene (RIP7) controlling the reverse tetracycline transactivator (rtTA), as well as the polyadenylation site sequence of the RIP7 gene was injected into fertilized (C57BL/6 X CBA)F2 mouse eggs. Founder line 2 with 10 copies of the transgene was established. When mice are crossed to transgenic mice carrying reporter genes, transgene expression is detected only in pancreatic islet beta cells. [MGI Ref ID J:130049] | ||
Genotyping Protocols
Tg(Ins2-rtTA)2Efr
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice can are bred as hemizygotes or homozygotes. |
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Related Strains
Strains carrying other alleles of Ins2
View Strains carrying other alleles of Ins2 (42 strains)
005670 B6.Cg-Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J 006235 B6.Cg-Tg(SFTPC-rtTA)5Jaw/J 006232 B6.Cg-Tg(Scgb1a1-rtTA)1Jaw/J 007678 B6;SJL-Tg(KRT14-rtTA)208Jek/J 006245 C.Cg-Tg(SFTPC-rtTA)5Jaw/J 006242 C.Cg-Tg(Scgb1a1-rtTA)1Jaw/J 008099 FVB-Tg(KRT14-rtTA)F42Efu/J 004127 FVB-Tg(Nes-rtTA)306Rvs/J 006225 FVB.Cg-Tg(SFTPC-rtTA)5Jaw/J 006222 FVB.Cg-Tg(Scgb1a1-rtTA)1Jaw/J 008202 FVB/N-Tg(NPHS2-rtTA2*M2)1Jbk/J 006875 FVB/N-Tg(Tagln-rtTA)E1Jwst/J 004602 NOD.Cg-Tg(Ins2-rtTA)2Doi/DoiJ 005734 NOD/Lt-Tg(Ins2-rtTA)1Ach/AchJ 005572 STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J 005493 STOCK Tg(Tek-rtTA,TRE-lacZ)1425Tpr/J 003273 STOCK Tg(rtTAhCMV)4Bjd/J
Additional Web Information
Tet Expression Systems
Research Applications
This mouse can be used to support research in many areas including:
Research Tools
Tet Expression Systems (tTA/rtTA Expressing Strains)
References
Selected Reference(s)
Additional ReferencesMilo-Landesman D; Surana M; Berkovich I; Compagni A; Christofori G; Fleischer N; Efrat S. 2001. Correction of hyperglycemia in diabetic mice transplanted with reversibly immortalized pancreatic beta cells controlled by the tet-on regulatory system. Cell Transplant 10(7):645-50. [PubMed: 11714200] [MGI Ref ID J:130049]
Price and Supply Information
| Strain Name: | STOCK Tg(Ins2-rtTA)2Efr/J |
| Stock Number: | 008250 |
This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date:
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
View All Strains Under Development
Supply Details
| Standard Supply | Under Development for Distribution Colony |
|---|---|
| Supply Notes |
This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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