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| Ins-rtTA (or RIP7-rtTA) transgenic mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the rat insulin 2 (Ins2) promoter. When mated to a second transgenic strain carrying a gene under the regulatory control of a tetracycline-responsive promoter element (TRE or tetO), expression of the gene in pancreatic beta cells is induced with administration of the tetracycline analog, doxycycline (dox). This strain provides a Tet-On tool that allows the inducible expression of genes in pancreatic beta cells. | ||||||||||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Douglas Melton, Harvard University Description
These mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the rat insulin 2 (Ins2) promoter. When mated to a second transgenic strain carrying a gene under the regulatory control of a tetracycline-responsive promoter element (tetO), expression of the gene may be regulated by the tetracycline analog, doxycycline (dox); in the presence of dox, transcription of the target gene is induced in pancreatic beta cells where rtTA is expressed. Induction of rtTA activity was not detected in other tissues. This strain provides a Tet-On tool that allows the inducible expression of genes in pancreatic beta cells. The Donating Investigator reports that homozygotes are viable and fertile.Development
A transgenic construct containing the reverse tetracycline-controlled transactivator (rtTA) gene, 9.5kb 5' regulatory sequence and first intron of the rat insulin 2 gene (RIP7) and polyadenylation site sequence was injected into fertilized (C57BL/6 X CBA)F2 mouse eggs. Founder line 2 with 10 copies of the transgene was established and backcrossed to C57BL/6 for at least 3 generations. The mice were then maintained on the outbred ICR background. The mice were then crosssed to C57BL/6J once at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Tg(Ins2-rtTA)2Efr allele
008755 STOCK Tg(Ins2-rtTA)2Efr Tg(teto-DTA)1Gfi/J View Strains carrying Tg(Ins2-rtTA)2Efr (1 strain)
008755 STOCK Tg(Ins2-rtTA)2Efr Tg(teto-DTA)1Gfi/J
Additional Web Information
Tet Expression Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Research Tools
Tet Expression Systems
tTA/rtTA Expressing Strains
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Ins2-rtTA)2Efr | ||
|---|---|---|---|
| Allele Name | transgene insertion 2, Shimon Efrat | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | Ins-rtTA; RIP7-rtTA; RiprtTA; | ||
| Strain of Origin | (C57BL/6 x CBA)F2 | ||
| Site of Expression | pancreatic beta cells | ||
| Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli | ||
| The tetracycline repressor gene (Tetr), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). One mutant with a four amino acid residue change (rTetR) exhibited dependence on tetracycline for induction of the targeted gene and was used in the rtTA construct (Gossen et al, 1995). rTetr was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). | |||
| Promoter | Ins2, insulin 2, rat | ||
| Molecular Note | A transgenic construct containing 9.5kb 5' regulatory sequence and first intron of the rat insulin 2 gene (RIP7) controlling the reverse tetracycline transactivator (rtTA), as well as the polyadenylation site sequence of the RIP7 gene was injected into fertilized (C57BL/6 X CBA)F2 mouse eggs. Founder line 2 with 10 copies of the transgene was established. When mice are crossed to transgenic mice carrying reporter genes, transgene expression is detected only in pancreatic islet beta cells. [MGI Ref ID J:130049] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Ins2-rtTA)2Efr, Standard PCR
Tg(tTA), Melt Curve Analysis
Tg(tTA), QPCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Milo-Landesman D; Surana M; Berkovich I; Compagni A; Christofori G; Fleischer N; Efrat S. 2001. Correction of hyperglycemia in diabetic mice transplanted with reversibly immortalized pancreatic beta cells controlled by the tet-on regulatory system. Cell Transplant 10(7):645-50. [PubMed: 11714200] [MGI Ref ID J:130049]
Additional References
Eldor R; Yeffet A; Baum K; Doviner V; Amar D; Ben-Neriah Y; Christofori G; Peled A; Carel JC; Boitard C; Klein T; Serup P; Eizirik DL; Melloul D. 2006. Conditional and specific NF-kappaB blockade protects pancreatic beta cells from diabetogenic agents. Proc Natl Acad Sci U S A 103(13):5072-7. [PubMed: 16551748] [MGI Ref ID J:107655]
Nir T; Melton DA; Dor Y. 2007. Recovery from diabetes in mice by beta cell regeneration. J Clin Invest 117(9):2553-61. [PubMed: 17786244] [MGI Ref ID J:127412]
Tg(Ins2-rtTA)2Efr relatedBalcazar N; Sathyamurthy A; Elghazi L; Gould A; Weiss A; Shiojima I; Walsh K; Bernal-Mizrachi E. 2009. mTORC1 activation regulates beta-cell mass and proliferation by modulation of cyclin D2 synthesis and stability. J Biol Chem 284(12):7832-42. [PubMed: 19144649] [MGI Ref ID J:148614]
Berkovich I; Efrat S. 2001. Inducible and Reversible beta-Cell Autoimmunity and Hyperplasia in Transgenic Mice Expressing a Conditional Oncogene. Diabetes 50(10):2260-7. [PubMed: 11574407] [MGI Ref ID J:71705]
Eldor R; Yeffet A; Baum K; Doviner V; Amar D; Ben-Neriah Y; Christofori G; Peled A; Carel JC; Boitard C; Klein T; Serup P; Eizirik DL; Melloul D. 2006. Conditional and specific NF-kappaB blockade protects pancreatic beta cells from diabetogenic agents. Proc Natl Acad Sci U S A 103(13):5072-7. [PubMed: 16551748] [MGI Ref ID J:107655]
Gupta S; McGrath B; Cavener DR. 2009. PERK regulates the proliferation and development of insulin-secreting beta-cell tumors in the endocrine pancreas of mice. PLoS One 4(11):e8008. [PubMed: 19956728] [MGI Ref ID J:155377]
Jaggi F; Cabrita MA; Perl AK; Christofori G. 2008. Modulation of endocrine pancreas development but not beta-cell carcinogenesis by Sprouty4. Mol Cancer Res 6(3):468-82. [PubMed: 18337453] [MGI Ref ID J:138091]
Nir T; Melton DA; Dor Y. 2007. Recovery from diabetes in mice by beta cell regeneration. J Clin Invest 117(9):2553-61. [PubMed: 17786244] [MGI Ref ID J:127412]
Porat S; Weinberg-Corem N; Tornovsky-Babaey S; Schyr-Ben-Haroush R; Hija A; Stolovich-Rain M; Dadon D; Granot Z; Ben-Hur V; White P; Girard CA; Karni R; Kaestner KH; Ashcroft FM; Magnuson MA; Saada A; Grimsby J; Glaser B; Dor Y. 2011. Control of pancreatic beta cell regeneration by glucose metabolism. Cell Metab 13(4):440-9. [PubMed: 21459328] [MGI Ref ID J:172243]
Sodir NM; Swigart LB; Karnezis AN; Hanahan D; Evan GI; Soucek L. 2011. Endogenous Myc maintains the tumor microenvironment. Genes Dev 25(9):907-16. [PubMed: 21478273] [MGI Ref ID J:171447]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice may be bred as hemizygotes or homozygotes.
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
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Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
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Terms of Use
Terms of Use
General Terms and Conditions
Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
Contact information
General inquiriesContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
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