Strain Name: |
B6.Cg-Tg(tetO-Clock)1Jt/J |
|---|---|
Stock Number: |
008278 |
Availability: | Under Development for Distribution Colony |
| To register your interest in this strain go to the Strain Interest Form. |
General Terms and Conditions |
| Former Name |
B6.Cg-Tg(tetO-Clock)isoJt/J (Changed: 12-MAR-08
) |
| Genes & Alleles | Clock; Tg(tetO-Clock)1Jt; tetO; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Noncarrier x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Joseph Takahashi, Northwestern University Generation N11+ (21-JUL-08) Strain Description
Homozygous transgenic mice are viable and fertile. These animals express the wildtype Clock gene under the control of a tetracycline-responsive promoter element (TRE; tetO). When hemizygotes are bred with another transgenic mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA) under the regulation of tissue-specific promoters, transgene expression can be conditionally regulated in the appropriate tissue of the bitransgenic offspring with the tetracycline analog, doxycycline. Transgenic expression of the Clock gene in the suprachiasmatic nucleus and brain of mice regulates the period length of circadian locomotor rhythms. This wildtype transgene shortens the period of circadian locomotor rhythms in mice, whereas overexpression of the Clockdelta19 mutant transgene (Stock No. 008277) lengthens the period. These effects can be inhibited by low doses of doxycyline in the drinking water. The effects of low, but not high, doses of doxycyline are completely reversible and lead to a rapid reactivation of the transgene.When bred to a strain expressing tTA in brain (see Stock No. 008284 for example), this mutant mouse strain may be useful in studies of circadian behavior.
Strain Development
A transgenic vector was designed to carry this full-length wildtype mouse gene downstream of a tetracycline-responsive promoter (TRE; tetO). The vector was introduced to fertilized CD1 oocytes. Transgene positive founders (line iso) were backcrossed to C57BL/6J for more than ten generations by the donating laboratory.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(tetO-Clock)1Jt | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Joseph S Takahashi | ||
| Common Name(s) | Tg(tetO-Clock)isoJt; tetO::Clockwt; | ||
| Strain of Origin | CD-1 | ||
| Expressed Gene | Clock, circadian locomoter output cycles kaput, mouse, laboratory | ||
| Promoter | tetO, tet operator, | ||
| Molecular Note | A transgenic vector was designed to carry the full-length wildtype mouse Clock gene downstream of a tetracycline-responsive promoter (TRE; tetO). Crossing these mice with mice expressing the tTA or reverse tetracycline-controlled transactivator protein under control of tissue-specific promoters, transgene expression can be regulated in appropriate tissues in the offspring with administration of the tet analog doxycycline. [MGI Ref ID J:120326] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Related Strains
Strains carrying other alleles of Clock
002923 C57BL/6J-Clockm1Jt/J View Strains carrying other alleles of Clock (1 strain)
Additional Web Information
Congenic Nomenclature
Research Applications
This mouse can be used to support research in many areas including:
Clock relatedNeurobiology Research
Behavioral and Learning Defects
Circadian Rhythms
Neurobiology Research
Behavioral and Learning Defects
Circadian Rhythms
Reproductive Biology Research
Fertility Defects
References
Selected Reference(s)
Hong HK; Chong JL; Song W; Song EJ; Jyawook AA; Schook AC; Ko CH; Takahashi JS. 2007. Inducible and reversible Clock gene expression in brain using the tTA system for the study of circadian behavior. PLoS Genet 3(2):e33. [PubMed: 17319750] [MGI Ref ID J:120326]
Price and Supply Information
| Strain Name: | B6.Cg-Tg(tetO-Clock)1Jt/J |
| Stock Number: | 008278 |
This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date:
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
View All Strains Under Development
Supply Details
| Standard Supply | Under Development for Distribution Colony |
|---|---|
| Supply Notes |
This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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