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| These animals express the mouse Clock gene under the control of a tetracycline-responsive promoter element (TRE; tetO). When hemizygotes are bred with another transgenic mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA) under the regulation of tissue-specific promoters, transgene expression can be conditionally regulated in the appropriate tissue of the bitransgenic offspring with the tetracycline analog, doxycycline. When bred to a strain expressing tTA in brain (see Stock No. 008284 for example), this mutant mouse strain may be useful in studies of circadian behavior. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names B6.Cg-Tg(tetO-Clock)1Jt/J (Changed: 04-NOV-08 ) B6.Cg-Tg(tetO-Clock)isoJt/J (Changed: 12-MAR-08 ) Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Joseph Takahashi, Univ Texas Southwestern Medical Ctr Description
Homozygous transgenic mice are viable and fertile. These animals express the wildtype Clock gene under the control of a tetracycline-responsive promoter element (TRE; tetO). When hemizygotes are bred with another transgenic mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA) under the regulation of tissue-specific promoters, transgene expression can be conditionally regulated in the appropriate tissue of the bitransgenic offspring with the tetracycline analog, doxycycline. Transgenic expression of the Clock gene in the suprachiasmatic nucleus and brain of mice regulates the period length of circadian locomotor rhythms. This wild-type transgene shortens the period of circadian locomotor rhythms in mice, whereas overexpression of the Clockdelta19 mutant transgene (Stock No. 008277) lengthens the period. These effects can be inhibited by low doses of doxycyline in the drinking water. The effects of low, but not high, doses of doxycyline are completely reversible and lead to a rapid reactivation of the transgene.When bred to a strain expressing tTA in brain (see Stock No. 008284 for example), this mutant mouse strain may be useful in studies of circadian behavior.
Development
A transgenic vector was designed to carry this full-length wildtype mouse gene downstream of a tetracycline-responsive promoter (TRE; tetO). The vector was introduced to fertilized C57BL/6 oocytes. Transgene positive founders (line iso) were maintained on a C57BL/6 background by the donating laboratory.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Clock
008277 B6.Cg-Tg(tetO-Clockm1Jt)CL57Jt/J 002923 C57BL/6J-Clockm1Jt/J View Strains carrying other alleles of Clock (2 strains)
Phenotype
Phenotype Information
assigned by genotype
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Tg(Scg2-tTA)1Jt/0 Tg(tetO-Clock)1Jt/0
involves: C57BL/6J * CD-1
- behavior/neurological phenotype
- shortened circadian period (MGI Ref ID J:120326)
- double transgenic mice display a circadian period that is shortened by 1 hour relative to wild-type mice
- treatment with doxycycline to suppress transgenic Clock expression demonstrates a rapid switch-off of expression and rapid turn-on upon return to normal water
This mouse can be used to support research in many areas including:
Clock relatedNeurobiology Research
Behavioral and Learning Defects
Circadian Rhythms
Neurobiology Research
Behavioral and Learning Defects
Circadian Rhythms
Reproductive Biology Research
Fertility Defects
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(tetO-Clock)1Jt | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Joseph S Takahashi | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | Tg(tetO-Clock)isoJt; tetO::Clockwt; | ||
| Strain of Origin | C57BL/6 | ||
| Expressed Gene | Clock, circadian locomoter output cycles kaput, mouse, laboratory | ||
| Promoter | tetO, tet operator, | ||
| Molecular Note | A transgenic vector was designed to carry the full-length wildtype mouse Clock gene downstream of a tetracycline-responsive promoter (TRE; tetO). Crossing these mice with mice expressing the tTA or reverse tetracycline-controlled transactivator protein under control of tissue-specific promoters, transgene expression can be regulated in appropriate tissues in the offspring with administration of the tet analog doxycycline. [MGI Ref ID J:120326] | ||
Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Hong HK; Chong JL; Song W; Song EJ; Jyawook AA; Schook AC; Ko CH; Takahashi JS. 2007. Inducible and reversible Clock gene expression in brain using the tTA system for the study of circadian behavior. PLoS Genet 3(2):e33. [PubMed: 17319750] [MGI Ref ID J:120326]
Health & husbandry
Health & Colony Maintenance Information
Currently there no information available for this strain. This may be due to the supply level of this strain.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
|
Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Contact Information
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Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
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Terms of Use
Terms of Use
General Terms and Conditions
Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
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