|These mice express tetracycline-controlled transactivator protein (tTA) under the control of a mouse secretogranin II promoter. When these mice are mated to a second transgenic strain that carries a gene of interest driven by a tetracycline-responsive promoter element (TRE; tetO), expression of that gene can be conditionally regulated by the presence or absence of doxycycline in the drinking water. Expression in the suprachiasmatic nucleus and brain of bitransgenic animals can be reversibly inhibited by the presence of doxycycline or induced by withdrawal of the tetracycline analog.|
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Donating Investigator Dr. Joseph S. Takahashi, Univ Texas Southwestern Medical Ctr
Homozygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These mice express tetracycline-controlled transactivator protein (tTA) under the control of a mouse secretogranin II promoter. When these mice are mated to a second transgenic strain that carries a gene of interest driven by a tetracycline-responsive promoter element (TRE; tetO), expression of that gene can be conditionally regulated by the presence or absence of doxycycline in the drinking water. Expression in the suprachiasmatic nucleus and brain of bitransgenic animals can be reversibly inhibited by the presence of doxycycline or induced by withdrawal of the tetracycline analog.
Strains carrying other alleles of tTA
008079 129S-Ppargtm2Yba/J 016198 129S6.Cg-Tg(Camk2a-tTA)1Mmay/JlwsJ 011008 B6.129P2(Cg)-Gt(ROSA)26Sortm1(tTA)Roos/J 009602 B6.129S4(Cg)-Kcnn2tm2Jpad/J 009603 B6.129S4-Kcnn3tm1Jpad/J 008227 B6.129S4-Ppargtm3Yba/J 012359 B6.Cg-Pvalbtm1.1(tTA2)Hze/J 016868 B6.Cg-Ssttm1.2(tTA2)Hze/J 007004 B6.Cg-Tg(Camk2a-tTA)1Mmay/DboJ 003563 B6.Cg-Tg(Cebpb-tTA)5Bjd/J 003767 B6.Cg-Tg(Eno2tTA)5021Nes/J 003763 B6.Cg-Tg(Eno2tTA)5030Nes/J 018306 B6.Cg-Tg(Fos-tTA,Fos-EGFP*)1Mmay/J 005964 B6.Cg-Tg(GFAP-tTA)110Pop/J 002618 B6.Cg-Tg(MMTVtTA)1Mam/J 023970 B6.Cg-Tg(Sirpa-tTA)AUmri/J 023971 B6.Cg-Tg(Sirpa-tTA)SUmri/J 006361 B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J 017722 B6.Cg-Tg(Tal1-tTA)19Dgt/J 017754 B6;129-Omptm1(tTA)Gogo/J 007585 B6;129S4-Npytm2Rpa/J 002709 B6;C3-Tg(TettTALuc)1Dgs/J 003010 B6;CBA-Tg(Camk2a-tTA)1Mmay/J 008344 B6;DBA-Tg(Fos-tTA,Fos-EGFP*)1Mmay Tg(tetO-lacZ,tTA*)1Mmay/J 010573 B6;SJL-Tg(Prl-tTA)6-5Jek/J 008082 B6;SJL-Tg(Tagln-tTA)1Mrab Tg(tetO-Mcpt1)1Mrab/J 008603 C.129P2(B6)-Gt(ROSA)26Sortm1(tTA)Roos/J 010712 C57BL/6-Tg(Camk2a-tTA)1Stl/J 013585 FVB-Tg(Cdh5-tTA)D5Lbjn/J 005625 FVB-Tg(Pcp2-tTA)3Horr/J 003170 FVB.Cg-Tg(Myh6-tTA)6Smbf/J 006209 FVB.Cg-Tg(Tal1-tTA)19Dgt/J 005942 FVB/N-Tg(Pf4-tTA/VP16)42Kra/J 004937 NOD.Cg-Tg(Ins2-tTA)1Doi/DoiJ 006999 STOCK Dbttm1Geh Tg(Cebpb-tTA)5Bjd Tg(tetO-DBT)A1Geh/J 008335 STOCK Foxa2tm1.1(rtTa)Moon/J 008600 STOCK Gt(ROSA)26Sortm1(tTA)Roos/J 005701 STOCK Pdx1tm1Macd/J 014092 STOCK Tg(ACTB-tTA2,-MAPT/lacZ)1Luo/J 003271 STOCK Tg(CMV-tTA)3Bjd/J 024854 STOCK Tg(Camk2a-tTA)1Mmay Tg(tetO-MAPT*P301L)#Kha/J 018124 STOCK Tg(Prnp-tTA)F959Sbp/J 009606 STOCK Tg(Six2-EGFP/cre)1Amc/J 003275 STOCK Tg(tetL)1Bjd/J 003274 STOCK Tg(tetNZL)2Bjd/J 016970 STOCK Tg(tetO-HCV)1Mlch/MmjaxView Strains carrying other alleles of tTA (46 strains)
View Research Applications
|Allele Name||transgene insertion 1, Joseph S Takahashi|
|Allele Type||Transgenic (Inserted expressed sequence)|
|Mutation Made By||Dr. Joseph Takahashi, Univ Texas Southwestern Medical Ctr|
|Strain of Origin||CD-1|
|Site of Expression||The transgene shows expression in the brain and is enriched in the suprachiasmatic nucleus.|
|Expressed Gene||tTA, tetracycline-controlled transactivator, E. coli|
|The tetracycline-resistance gene (TetR), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). TetR was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). The tetracycline-inhibitable transcription factor is a component of a bigenic system that allows doxycycline (a tetracycline analog) regulatable expression of genes that are under the direction of the tetracycline responsive promoter (TetOp)promoter.|
|Promoter||Scg2, secretogranin II, mouse, laboratory|
|Molecular Note||9.85 kb of DNA upstream from the mouse Scg2 translational start site was placed upstream of the tTA gene encoded in the pMMY20 plasmid. The transgene shows expression in the brain and is enriched in the suprachiasmatic nucleus. [MGI Ref ID J:116189]|
Hong HK; Chong JL; Song W; Song EJ; Jyawook AA; Schook AC; Ko CH; Takahashi JS. 2007. Inducible and reversible Clock gene expression in brain using the tTA system for the study of circadian behavior. PLoS Genet 3(2):e33. [PubMed: 17319750] [MGI Ref ID J:120326]
Hughes ME; Hong HK; Chong JL; Indacochea AA; Lee SS; Han M; Takahashi JS; Hogenesch JB. 2012. Brain-specific rescue of Clock reveals system-driven transcriptional rhythms in peripheral tissue. PLoS Genet 8(7):e1002835. [PubMed: 22844252] [MGI Ref ID J:188150]
McDearmon EL; Patel KN; Ko CH; Walisser JA; Schook AC; Chong JL; Wilsbacher LD; Song EJ; Hong HK; Bradfield CA; Takahashi JS. 2006. Dissecting the functions of the mammalian clock protein BMAL1 by tissue-specific rescue in mice. Science 314(5803):1304-8. [PubMed: 17124323] [MGI Ref ID J:116189]
Yoo SH; Mohawk JA; Siepka SM; Shan Y; Huh SK; Hong HK; Kornblum I; Kumar V; Koike N; Xu M; Nussbaum J; Liu X; Chen Z; Chen ZJ; Green CB; Takahashi JS. 2013. Competing E3 Ubiquitin Ligases Govern Circadian Periodicity by Degradation of CRY in Nucleus and Cytoplasm. Cell 152(5):1091-105. [PubMed: 23452855] [MGI Ref ID J:194037]
Animal Health ReportsProduction of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
|Pricing for USA, Canada and Mexico shipping destinations|
Cryopreserved Mice - Ready for Recovery
Price (US dollars $) Cryorecovery* $2140.00
At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
|Pricing for International shipping destinations|
Cryopreserved Mice - Ready for Recovery
Price (US dollars $) Cryorecovery* $2782.00
Cryorecovery - Standard.
Progeny testing is not required.
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