Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation +pN1 Generation Definitions   Donating Investigator Dr. Arthur Weiss,   University of California, San Francisco

Description
Gene targeting was used to remove a transmembrane domain from the targeted protein. Homozygotes exhibit a partial peripheral B cell development block at the first transitional stage (T1), although total T or B cell numbers in the spleen and lymph node are unchanged. Truncated product (mRNA) is detected by Northern blot analysis of splenocytes isolated from homozygous animals. A secreted protein without known physiologic consequences is detected in serum from homozygous animals, but no surface protein is detected on cells of hematopoietic lineage, including T cells, B cells, and myeloid cells. Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities; homozygotes are not fertile. This strain may be useful in studies of immune responses.

Development
A targeting vector was designed to introduce a loxP-flanked neomycin (neo) resistance cassette in reverse transcriptional orientation to intron 17 and an additional loxP site to intron 18. Exon 18 encodes the transmembrane domain of the gene (also called CD148). The targeting construct was transfected into 129P2/OlaHsd-derived E14.1TG3B1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. Targeted animals were bred with BALB/c-Tg(CMV-cre)1Cgn/J female mice (Stock No. 003465) to remove the floxed sequences. The resulting offspring contained multiple gene rearrangments; intact floxed-exon 18, intact floxed-neo cassette, or excision of both exon 18 and the neo cassette. Correctly targeted progeny, lacking exon 18 and the neo cassette, were bred to remove the CMV-Cre transgene. This line was backcrossed to C57BL/6 for at least 12 generations by the donating laboratory. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.

Control Information

	Control
	Wild-type from the colony
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying   Ptprj^tm1.2Weis allele

008290

C.129P2-Ptprjtm1.2Weis/J

View Strains carrying   Ptprj^tm1.2Weis     (1 strain)

Strains carrying other alleles of Ptprj

008291

B6.129P2(C)-Ptprjtm1.1Weis/J

View Strains carrying other alleles of Ptprj     (1 strain)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI

- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Colorectal Cancer; CRC   (PTPRJ)

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Ptprj^tm1.2Weis/Ptprj^tm1.2Weis

        involves: 129P2/OlaHsd * BALB/cJ * C57BL/6

• hematopoietic system phenotype
• abnormal B cell differentiation
  • partial block at T1 stage is observed   (MGI Ref ID J:131736)
• abnormal splenic cell ratio
  • spleens contain more T1 B cells (IgM^lo IgD^hi) and fewer follicular mature (FM, IgM^hi IgD^lo) B cells than controls   (MGI Ref ID J:131736)
• increased B-2 B cell number
  • skewing toward B-2 lineage at expense of B-1 lineage is observed in peritoneal lavage B cells   (MGI Ref ID J:131736)
• increased marginal zone B cell number
  • mice have more splenic marginal zone B cells than controls   (MGI Ref ID J:131736)
• reproductive system phenotype
• infertility
  • homozygous mice are infertile; line is maintained as heterozygotes   (MGI Ref ID J:131736)
• immune system phenotype
• abnormal B cell differentiation
  • partial block at T1 stage is observed   (MGI Ref ID J:131736)
• abnormal splenic cell ratio
  • spleens contain more T1 B cells (IgM^lo IgD^hi) and fewer follicular mature (FM, IgM^hi IgD^lo) B cells than controls   (MGI Ref ID J:131736)
• increased B-2 B cell number
  • skewing toward B-2 lineage at expense of B-1 lineage is observed in peritoneal lavage B cells   (MGI Ref ID J:131736)
• increased marginal zone B cell number
  • mice have more splenic marginal zone B cells than controls   (MGI Ref ID J:131736)
• cellular phenotype
• abnormal B cell differentiation
  • partial block at T1 stage is observed   (MGI Ref ID J:131736)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
Immunodeficiency
      B cell deficiency

Reproductive Biology Research
Fertility Defects

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Ptprjtm1.2Weis
Allele Name		targeted mutation 1.2, Arthur Weiss
Allele Type		Targeted (knock-out)
Common Name(s)		CD148-; PtprjTM-;
Mutation Made By		Dr. Arthur Weiss,   University of California, San Francisco
Strain of Origin		129P2/OlaHsd
ES Cell Line Name		E14.1TG3B1
ES Cell Line Strain		129P2/OlaHsd
Gene Symbol and Name		Ptprj, protein tyrosine phosphatase, receptor type, J
Chromosome		2
Gene Common Name(s)		AI450271; Byp; CD148; DEP-1; DEP1; HPTPeta; R-PTP-ETA; RPTPJ; SCC1; Scc-1; Scc1; colon tumor susceptibility 1; expressed sequence AI450271;
Molecular Note		A targeting vector was designed to introduce a loxP-flanked neomycin-resistance cassette in reverse transcriptional orientation to intron 17 and an additional loxP site to intron 18. Exon 18 encodes the transmembrane domain of the gene (also called CD148). Targeted animals were bred with BALB/c-background CMV-Cre transgenic female mice to delete the neomycin cassette and exon 18. Transmembrane domain-deleted mice were obtained and characterized. CD148 protein is not detected on any cells of hematopoieticorigin by immunoblotting or flow cytometry. [MGI Ref ID J:131736]

Genotyping

Genotyping Information

Genotyping Protocols

Ptprj^tm1.2Weis, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Zhu JW; Brdicka T; Katsumoto TR; Lin J; Weiss A. 2008. Structurally distinct phosphatases CD45 and CD148 both regulate B cell and macrophage immunoreceptor signaling. Immunity 28(2):183-96. [PubMed: 18249142]  [MGI Ref ID J:131736]

Additional References

Ptprj^tm1.2Weis related

Severin S; Pollitt AY; Navarro-Nunez L; Nash CA; Mourao-Sa D; Eble JA; Senis YA; Watson SP. 2011. Syk-dependent phosphorylation of CLEC-2: a novel mechanism of hem-immunoreceptor tyrosine-based activation motif signaling. J Biol Chem 286(6):4107-16. [PubMed: 21098033]  [MGI Ref ID J:169492]

Zhu JW; Doan K; Park J; Chau AH; Zhang H; Lowell CA; Weiss A. 2011. Receptor-like Tyrosine Phosphatases CD45 and CD148 Have Distinct Functions in Chemoattractant-Mediated Neutrophil Migration and Response to S. aureus. Immunity 35(5):757-69. [PubMed: 22078799]  [MGI Ref ID J:178830]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & Husbandry	When maintained as a live colony, heterozygotes may be bred. Homozygous males and females are not fertile.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Control Information

	Control
	Wild-type from the colony
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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