Strain Name:

C.129P2-Ptprjtm1.2Weis/J

Stock Number:

008290

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Gene targeting was used to remove a transmembrane domain from the expressed protein. Homozygotes exhibit a partial peripheral B cell development block at the first transitional stage (T1), although total T or B cell numbers in the spleen and lymph node are unchanged. Truncated product (mRNA) is detected by Northern blot analysis of splenocytes isolated from homozygous animals. A secreted protein without known physiologic consequences is detected in serum from homozygous animals, but no surface protein is detected on cells of hematopoietic lineage, including T cells, B cells, and myeloid cells. This strain may be useful for studies of immune responses.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Specieslaboratory mouse
Generation+pN1
Generation Definitions
 
Donating InvestigatorDr. Arthur Weiss,   University of California, San Francisco

Description
Gene targeting was used to remove a transmembrane domain from the expressed protein. Homozygotes exhibit a partial peripheral B cell development block at the first transitional stage (T1), although total T or B cell numbers in the spleen and lymph node are unchanged. Truncated product (mRNA) is detected by Northern blot analysis of splenocytes isolated from homozygous animals. A secreted protein without known physiologic consequences is detected in serum from homozygous animals, but no surface protein is detected on cells of hematopoietic lineage, including T cells, B cells, and myeloid cells. Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities; homozygotes are not fertile. Mice on the BALB/c background develop splenomegaly with age (not observed on the C57BL/6 background (Stock No. 008289)). This strain may be useful in studies of immune responses.

Development
A targeting vector was designed to introduce a loxP-flanked neomycin-resistance cassette in reverse transcriptional orientation to intron 17 and an additional loxP site to intron 18. Exon 18 encodes the transmembrane domain of the gene (also called CD148). The targeting construct was transfected into E14.1TG3B1 129P2/OlaHsd-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. Targeted animals were bred with BALB/c-background CMV-Cre transgenic female mice. Progeny lacking exon 18 and the neomycin cassette (one loxP site remaining) were bred to remove the CMV-Cre transgene. This line was backcrossed to BALB/c for ten generations by the donating laboratory.

Related Strains

Strains carrying   Ptprjtm1.2Weis allele
008289   B6.129P2(C)-Ptprjtm1.2Weis/J
View Strains carrying   Ptprjtm1.2Weis     (1 strain)

Strains carrying other alleles of Ptprj
008291   B6.129P2(C)-Ptprjtm1.1Weis/J
View Strains carrying other alleles of Ptprj     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Ptprjtm1.2Weis/Ptprjtm1.2Weis

        involves: 129P2/OlaHsd * BALB/cJ * C57BL/6
  • hematopoietic system phenotype
  • abnormal B cell differentiation
    • partial block at T1 stage is observed   (MGI Ref ID J:131736)
  • abnormal splenic cell ratio
    • spleens contain more T1 B cells (IgMlo IgDhi) and fewer follicular mature (FM, IgMhi IgDlo) B cells than controls   (MGI Ref ID J:131736)
  • increased B-2 B cell number
    • skewing toward B-2 lineage at expense of B-1 lineage is observed in peritoneal lavage B cells   (MGI Ref ID J:131736)
  • increased marginal zone B cell number
    • mice have more splenic marginal zone B cells than controls   (MGI Ref ID J:131736)
  • reproductive system phenotype
  • infertility
    • homozygous mice are infertile; line is maintained as heterozygotes   (MGI Ref ID J:131736)
  • immune system phenotype
  • abnormal B cell differentiation
    • partial block at T1 stage is observed   (MGI Ref ID J:131736)
  • abnormal splenic cell ratio
    • spleens contain more T1 B cells (IgMlo IgDhi) and fewer follicular mature (FM, IgMhi IgDlo) B cells than controls   (MGI Ref ID J:131736)
  • increased B-2 B cell number
    • skewing toward B-2 lineage at expense of B-1 lineage is observed in peritoneal lavage B cells   (MGI Ref ID J:131736)
  • increased marginal zone B cell number
    • mice have more splenic marginal zone B cells than controls   (MGI Ref ID J:131736)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
Immunodeficiency
      B cell deficiency

Reproductive Biology Research
Fertility Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Ptprjtm1.2Weis
Allele Name targeted mutation 1.2, Arthur Weiss
Allele Type Targeted (Null/Knockout)
Common Name(s) CD148-; PtprjTM-;
Mutation Made ByDr. Arthur Weiss,   University of California, San Francisco
Strain of Origin129P2/OlaHsd
ES Cell Line NameE14.1TG3B1
ES Cell Line Strain129P2/OlaHsd
Gene Symbol and Name Ptprj, protein tyrosine phosphatase, receptor type, J
Chromosome 2
Gene Common Name(s) AI450271; Byp; CD148; DEP-1; DEP1; HPTPeta; R-PTP-ETA; RPTPJ; SCC1; Scc-1; Scc1; colon tumor susceptibility 1; expressed sequence AI450271;
Molecular Note A targeting vector was designed to introduce a loxP-flanked neomycin-resistance cassette in reverse transcriptional orientation to intron 17 and an additional loxP site to intron 18. Exon 18 encodes the transmembrane domain of the gene (also called CD148). Targeted animals were bred with BALB/c-background CMV-Cre transgenic female mice to delete the neomycin cassette and exon 18. Transmembrane domain-deleted mice were obtained and characterized. CD148 protein is not detected on any cells of hematopoieticorigin by immunoblotting or flow cytometry. [MGI Ref ID J:131736]

Genotyping

Genotyping Information

Genotyping Protocols

Ptprjtm1.2Weis, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Zhu JW; Brdicka T; Katsumoto TR; Lin J; Weiss A. 2008. Structurally distinct phosphatases CD45 and CD148 both regulate B cell and macrophage immunoreceptor signaling. Immunity 28(2):183-96. [PubMed: 18249142]  [MGI Ref ID J:131736]

Additional References

Ptprjtm1.2Weis related

Katsumoto TR; Kudo M; Chen C; Sundaram A; Callahan EC; Zhu JW; Lin J; Rosen CE; Manz BN; Lee JW; Matthay MA; Huang X; Sheppard D; Weiss A. 2013. The phosphatase CD148 promotes airway hyperresponsiveness through SRC family kinases. J Clin Invest 123(5):2037-48. [PubMed: 23543053]  [MGI Ref ID J:201353]

Mori J; Wang YJ; Ellison S; Heising S; Neel BG; Tremblay ML; Watson SP; Senis YA. 2012. Dominant role of the protein-tyrosine phosphatase CD148 in regulating platelet activation relative to protein-tyrosine phosphatase-1B. Arterioscler Thromb Vasc Biol 32(12):2956-65. [PubMed: 23065825]  [MGI Ref ID J:208313]

Severin S; Pollitt AY; Navarro-Nunez L; Nash CA; Mourao-Sa D; Eble JA; Senis YA; Watson SP. 2011. Syk-dependent phosphorylation of CLEC-2: a novel mechanism of hem-immunoreceptor tyrosine-based activation motif signaling. J Biol Chem 286(6):4107-16. [PubMed: 21098033]  [MGI Ref ID J:169492]

Zhu JW; Doan K; Park J; Chau AH; Zhang H; Lowell CA; Weiss A. 2011. Receptor-like Tyrosine Phosphatases CD45 and CD148 Have Distinct Functions in Chemoattractant-Mediated Neutrophil Migration and Response to S. aureus. Immunity 35(5):757-69. [PubMed: 22078799]  [MGI Ref ID J:178830]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintained as a live colony, heterozygotes may be bred. Homozygotes are not fertile.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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