Strain Name: |
B6;129-Syt9tm1Sud/J |
|---|---|
Stock Number: |
008295 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Syt9; Syt9tm1Sud; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Mating System Heterozygote x Heterozygote (Female x Male) Species laboratory mouse Donating Investigator Thomas Sudhof, Stanford University School of Medicine Generation ?+N1F1 (28-JUL-08) Strain Description
Mice homozygous for this targeted knock-in are viable and fertile and do not display any gross physical or behavioral abnormalities.Synaptotagmin IX knock-in mice contain GFP "emerald" fused to the first exon of the targeted gene, and exon 2 is flanked by loxP sites. GFP expression is primarily localized in the limbic system and striatum of the brain. When crossed with a Cre recombinase-expressing strain, offspring are produced with tissue-specific deletion of Syt9 and which lack GFP expression, suggesting this creates a null allele. This strain may be used to study Ca2+ sensors involved with fast neurotransmitter release. Synaptotagmin IX knock-in mice contain GFP "Strain Development
A targeting vector was designed to fuse a signal peptide from rat neurexin 1 alpha followed by a GFP "emerald" cassette to the amino(N) terminus of exon 1, flank exon 2 with loxP sites, and insert an Flp-flanked neomycin resistance cassette in intron 2. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. Resultant mice were crossed with an FRT recombinase strain to remove the FLP-flanked neomycin cassette. This strain was maintained on a mixed C57BL/6 and 129 background by the donating laboratory.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Syt9tm1Sud | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Thomas C Sudhof | ||
| Common Name(s) | S9F; | ||
| Mutation Made By | Thomas Sudhof, Stanford University School of Medicine | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | GFP "emerald" expression is primarily localized in the limbic system and striatum of the brain. When crossed with a Cre recombinase-expressing strain, tissue-specific expression can be eliminated. | ||
| Gene Symbol and Name | Syt9, synaptotagmin IX | ||
| Chromosome | 7 | ||
| Gene Common Name(s) | FLJ45896; Sytv; | ||
| Molecular Note | A targeting vector was designed to fuse a signal peptide from rat neurexin 1 alpha followed by a GFP "emerald" cassette to the amino(N) terminus of exon 1, flank exon 2 with loxP sites, and insert an Flp-flanked neomycin resistance cassette in intron 2. Resultant mice were crossed with an FRT recombinase strain to remove the FLP-flanked neomycin cassette. Presence of the GFP fusion protein was detected by western blot analysis. [MGI Ref ID J:122459] | ||
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
Colony Maintenance
| Breeding & Husbandry | When maintained as a live colony, heterozygotes or homozygotes may be bred. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (145 strains)
Additional Web Information
Cre-lox or FLP-FRT Systems
Fluorescent Proteins/lacZ Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
Neurobiology Research
Cre-lox System (loxP-flanked Sequences)
Fluorescent protein expression in neural tissue
Neurotransmitter Receptor and Synaptic Vesicle Defects
Research Tools
Cre-lox System (loxP-flanked Sequences)
Fluorescent Proteins
References
Selected Reference(s)
Xu J; Mashimo T; Sudhof TC. 2007. Synaptotagmin-1, -2, and -9: Ca(2+) sensors for fast release that specify distinct presynaptic properties in subsets of neurons. Neuron 54(4):567-81. [PubMed: 17521570] [MGI Ref ID J:122459]
Price and Supply Information
| Strain Name: | B6;129-Syt9tm1Sud/J |
| Stock Number: | 008295 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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