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| The transgene in these mice is composed of a human neurofilament light polypeptide (NEFL) promoter driving the expression of an enhanced yellow fluorescent protein-mouse neurofilament heavy chain (Nefh) fusion protein. Hemizygous mice exhibit fluorescence in axonal fibers, including those of sciatic nerves, optical nerves, and spinal cord. | |||||||||||||||
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Description
Strain Information
Former Names B6.Cg-Tg(NEFL-EYFP-Nefh)40Gsn/J (Changed: 21-JUL-08 ) C57BL/6-Tg(NEFL-EYFP-Nefh)40Gsn/J (Changed: 18-JUL-08 ) B6.129-Tg(NEFL-EYFP-Nefh)40Gsn/J (Changed: 09-JUL-08 ) Type Congenic; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Noncarrier x Hemizygote (Female x Male) 04-MAR-09 Species laboratory mouse Generation N5+ (05-FEB-09) Donating Investigator Lawrence Goldstein, UCSD-HHMI Description
The transgene in these mice is composed of a human neurofilament light polypeptide (NEFL) promoter driving the expression of an enhanced yellow fluorescent protein-mouse neurofilament heavy chain (Nefh) fusion protein. Hemizygous mice exhibit fluorescence in axonal fibers, including those of sciatic nerves, optical nerves, and spinal cord.Development
A vector carrying a human neurofilament light polypeptide (NEFL) promoter driving the expression of an enhanced yellow fluorescent protein-mouse neurofilament heavy chain (Nefh) fusion protein was created. The transgenic vector was probably injected into the C57BL/6 x BALB/c(?) F1 blastocysts. Offspring showing germline transmission were crossed to C57BL/6NCrl for four generations by the donating laboratory.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
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005483 129-Tg(CAG-EYFP)7AC5Nagy/J 006148 B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J 007901 B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J 007911 B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J 007921 B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J 007612 B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J 007615 B6.Cg-Tg(Thy1-COP4/EYFP)9Gfng/J 005630 B6.Cg-Tg(Thy1-EYFP)15Jrs/J 003709 B6.Cg-Tg(Thy1-YFP)16Jrs/J 005627 B6.Cg-Tg(Thy1-YFP/Syp)10Jrs/J 003782 B6.Cg-Tg(Thy1-YFPH)2Jrs/J 007606 B6.Cg-Tg(Thy1-cre/ESR1,-EYFP)AGfng/J 008636 B6;C-Tg(Prnp-APP695*/EYFP)49Gsn/J 007910 B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J 005620 B6;D2-Tg(S100B-EYFP)1Wjt/J 007610 B6;SJL-Tg(Thy1-cre/ESR1,-EYFP)VGfng/J 007880 B6SJL-Tg(Thy1-Stx1a/EYFP)1Sud/J 006618 C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J 006362 C57BL/6J-Tg(CMV-Cox8a/EYFP)17J/J 007857 C57BL/6J-Tg(Eno2-YFP/Cox8a)YRwb/J 007860 C57BL/6J-Tg(Eno2-YFP/Cox8a)ZRwb/J 009422 NOD.Cg-Tg(Itgax-EYFP)1Mnz/QtngJ 005130 STOCK Gt(ROSA)26Sortm1(Smo/EYFP)Amc/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
YFP relatedNeurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Fluorescent Proteins
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(NEFL-EYFP/Nefh)40Gsn | ||
|---|---|---|---|
| Allele Name | transgene insertion 40, Lawrence SB Goldstein | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | EYFP-NFH; | ||
| Strain of Origin | (C57BL/6 x BALB/c)F1 | ||
| Site of Expression | axonal fibers of sciatic nerves, optical nerves, and spinal cord | ||
| Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish | ||
| Yellow Fluorescent Protein (YFP) is a derivative of Green Fluorescent Protein (GFP), a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance fluorescence intensity and shift the wavelength emitted when excited. When YFP is utilized in a transgenic construct, tissue expressing sufficient amounts of YFP will fluoresce yellowish-green when exposed to a 513 nm light source. | |||
| Expressed Gene | Nefh, neurofilament, heavy polypeptide, mouse, laboratory | ||
| Promoter | NEFL, neurofilament, light polypeptide, human | ||
| Molecular Note | The transgene construct was created from a vector carrying a human neurofilament light polypeptide (NEFL) promoter driving the expression of an enhanced yellow fluorescent protein-mouse neurofilament heavy chain (Nefh) fusion protein. Fluorescence was detected in axonal fibers, including those of sciatic nerves, optical nerves, and spinal cord. [MGI Ref ID J:133398] | ||
| Gene Symbol and Name | Tg(NEFL-EYFP/Nefh)40Gsn, transgene insertion 40, Lawrence SB Goldstein | ||
| Chromosome | UN | ||
| Gene Common Name(s) | EYFP-NFH; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(NEFL-EYFP/Nefh), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Additional References
Tg(NEFL-EYFP/Nefh)40Gsn relatedGoldstein LSB. 2008. Generation of a neurofilament light chain promoter-driven fluorescent reporter transgene MGI Direct Data Submission :. [MGI Ref ID J:133398]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number MGL375/MGL377
Colony Maintenance
Mating System Noncarrier x Hemizygote (Female x Male) 04-MAR-09
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(NEFL-EYFP/Nefh)40Gsn
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Tg(NEFL-EYFP/Nefh)40Gsn x Noncarrier $297.85 Noncarrier x Hemizygous for Tg(NEFL-EYFP/Nefh)40Gsn
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(NEFL-EYFP/Nefh)40Gsn
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Tg(NEFL-EYFP/Nefh)40Gsn x Noncarrier $387.30 Noncarrier x Hemizygous for Tg(NEFL-EYFP/Nefh)40Gsn
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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