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| This targeted mutant strain carries a loxP-flanked stop element in exon 1 and a G12D activating mutation in exon 2 of the neuroblastoma ras oncogene (Nras). This mutation functions as a null allele and has no apparent phenotype. Homozygotes are viable. Cre mediated excision of the floxed stop element causes a significant increase in GTP-bound N-Ras production. This conditional mutant strain may be helpful in further studies of this oncogene. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Coisogenic; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Dr. Tyler Jacks, Massachusetts Institute of Technology Description
This targeted mutant strain carries a loxP-flanked stop element in exon 1 and a G12D activating mutation in exon 2 of the neuroblastoma ras oncogene (Nras). This mutation functions as a null allele and has no apparent phenotype. Homozygotes are viable. Cre mediated excision of the floxed stop element causes a significant increase in GTP-bound N-Ras production. This conditional mutant strain may be helpful in further studies of this oncogene.For example, when crossed to a strain expressing Cre recombinase in epiblast and ectoderm-derived tissues (see Stock No. 003755), this mutant mouse strain may be useful in studies of the role of NRAS in leukemogenesis.
When crossed to a strain expressing interferon-induced Cre recombinase (see Stock No. 003556), this mutant mouse strain may be useful in studies of leukemia.
Development
A targeting vector was designed to include a lox-stop-lox element in intron 1 (to suppress expression in the absence of Cre recombinase) and a G12D activating mutation in exon 2. The vector was intoduced into V6.5 or V26.2 C57BL/6-derived embryonic stem cells. This strain has been maintained on a C57BL/6 background.
Control Information
| Control | ||
|---|---|---|
| 005304 C57BL/6NJ | ||
| Considerations for Choosing Controls | ||
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
View Related Disease (OMIM) Terms
Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Autoimmune Lymphoproliferative Syndrome, Type IV; ALPS4 (NRAS)
Colorectal Cancer; CRC (NRAS)
Nevus, Epidermal (NRAS)
Noonan Syndrome 6; NS6 (NRAS)
Thyroid Carcinoma, Follicular; FTC (NRAS)
assigned by genotype
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Nrastm1Tyj/Nrastm1Tyj Tg(Mx1-cre)1Cgn/?
involves: C57BL/6 * CBA (conditional)
- hematopoietic system phenotype
- abnormal myelopoiesis
- display a myeloproliferative phenotype 2 days after pIpC induction (MGI Ref ID J:174880)
- enlarged spleen
- marked splenomegaly 2 days after pIpC induction (MGI Ref ID J:174880)
- immune system phenotype
- abnormal myelopoiesis
- display a myeloproliferative phenotype 2 days after pIpC induction (MGI Ref ID J:174880)
- enlarged spleen
- marked splenomegaly 2 days after pIpC induction (MGI Ref ID J:174880)
Nrastm1Tyj/Nras+ Meox2tm1(cre)Sor/Meox2+
involves: 129S4/SvJaeSor * C57BL/6 (conditional)
- mortality/aging
- complete prenatal lethality
- no mice born alive (MGI Ref ID J:174880)
This mouse can be used to support research in many areas including:
Cancer Research
Oncogenes
Research Tools
Cre-lox System
loxP-flanked Sequences
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Nrastm1Tyj | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Tyler Jacks | ||
| Allele Type | Targeted (Floxed/Frt) | ||
| Common Name(s) | NrasG12D; NrasLSL-G12D; | ||
| Mutation Made By | Dr. Tyler Jacks, Massachusetts Institute of Technology | ||
| Strain of Origin | C57BL/6 | ||
| ES Cell Line Name | V26.2 | ||
| ES Cell Line Strain | C57BL/6 | ||
| Gene Symbol and Name | Nras, neuroblastoma ras oncogene | ||
| Chromosome | 3 | ||
| Gene Common Name(s) | ALPS4; AV095280; N-ras; NRAS1; NS6; expressed sequence AV095280; | ||
| Molecular Note | A targeting vector was designed to include a lox-stop-lox element in intron 1 (to suppress expression in the absence of Cre recombinase) and a G12D activating mutation in exon 2. [MGI Ref ID J:132357] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Nrastm1Tyj, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Haigis KM; Kendall KR; Wang Y; Cheung A; Haigis MC; Glickman JN; Niwa-Kawakita M; Sweet-Cordero A; Sebolt-Leopold J; Shannon KM; Settleman J; Giovannini M; Jacks T. 2008. Differential effects of oncogenic K-Ras and N-Ras on proliferation, differentiation and tumor progression in the colon. Nat Genet 40(5):600-8. [PubMed: 18372904] [MGI Ref ID J:132357]
Additional References
Nrastm1Tyj relatedLi Q; Haigis KM; McDaniel A; Harding-Theobald E; Kogan SC; Akagi K; Wong JC; Braun BS; Wolff L; Jacks T; Shannon K. 2011. Hematopoiesis and leukemogenesis in mice expressing oncogenic NrasG12D from the endogenous locus. Blood 117(6):2022-32. [PubMed: 21163920] [MGI Ref ID J:169608]
Wang J; Liu Y; Li Z; Du J; Ryu MJ; Taylor PR; Fleming MD; Young KH; Pitot H; Zhang J. 2010. Endogenous oncogenic Nras mutation promotes aberrant GM-CSF signaling in granulocytic/monocytic precursors in a murine model of chronic myelomonocytic leukemia. Blood 116(26):5991-6002. [PubMed: 20921338] [MGI Ref ID J:167384]
Wang J; Liu Y; Li Z; Wang Z; Tan LX; Ryu MJ; Meline B; Du J; Young KH; Ranheim E; Chang Q; Zhang J. 2011. Endogenous oncogenic Nras mutation initiates hematopoietic malignancies in a dose- and cell type-dependent manner. Blood 118(2):368-79. [PubMed: 21586752] [MGI Ref ID J:174880]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| 005304 C57BL/6NJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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For Licensing and Use Restrictions view the link(s) below:
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Contact information
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| phone: | 207-288-6470 |
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JAX® Mice, Products & Services Conditions of Use
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