Go to JAX^® Mice Query Form

General Terms and Conditions	Additional Licensing and Use Restrictions

Genes & Alleles

Gabrb3;   Gabrb3tm2.1Geh;

---

Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Donating Investigator Gregg Homanics,   University of Pittsburgh

Strain Description
These mice possess loxP sites on either side of exon 3 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissue(s).

When bred to a strain expressing Cre recombinase in the neuronal cells (see Stock No. 003966 for example), this mutant mouse strain may be useful in studies of neurodevelopmental diseases.

When bred to a strain expressing Cre recombinase in the hippocampal CA1 pyramidal cell layer (see Stock No. 005359 for example), this mutant mouse strain may be useful in studies of neurodevelopmental diseases.

Of note, several strains bearing gamma-aminobutyric acid (GABA-A) receptor mutations are available from this donating investigator (Dr. Gregg Homanics, University of Pittsburgh), including Gabra1 (Stock No. 004318), Gabra4 (Stock No. 006874), Gabra6 (Stock No. 002710), Gabrb3 (Stock No. 002711 and Stock No. 008310), Gabrd (Stock No. 003725), and Gabrg2 (Stock No. 003137).

Strain Development
A FRT site flanked targeting vector containing the neomycin resistance gene was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 3 of the targeted gene, and another loxP site was inserted upstream of exon 3. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected in C57BL/6J blastocysts. Resulting chimeric male animals were backcrossed to wildtype C57BL/6J female mice. The mice were then crossed to the FLP recombinase expressing strain B6;SJL-Tg(ACTFLPe)9205Dym/J (Stock No. 003800) to excise the neo cassette. The Tg(ACTFLPe)9205Dym transgene was bred out of the strain by backcrossing the mice to C57BL/6J for one additional generation. The mice retain one FRT site and loxP sites flanking exon 3.

Mammalian Phenotype Terms assigned by genotype The following phenotype information may relate to a genetic background differing from this JAX® Mice strain. Gabrb3tm2.1Geh/Gabrb3tm2.1Geh         involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL normal phenotype no abnormal phenotype detected (MGI Ref ID J:127877) mice were born at the expected frequency, were viable, did not have cleft palate, and were overtly indistinguishable from control litter-mates The following phenotype relates to a compound genotype created using this strain. Contact JAX® Services jaxservices@jax.org for customized breeding options. Gabrb3tm2.1Geh/Gabrb3tm2.1Geh Tg(Camk2a-cre)T29-1Stl/?         involves: 129 * BALB/c * C57BL * SJL   (conditional) lethality-postnatal postnatal lethality (MGI Ref ID J:127877) born at expected Mendelian frequency no cleft palate found about 30% of them die between 15 and 25 days after birth 72% of mice that died prematurely were male behavior/neurological phenotype abnormal behavior (MGI Ref ID J:127877) hyperresponsive to human contact abnormal maternal nurturing (MGI Ref ID J:127877) failed to care for pups absence seizures (MGI Ref ID J:127877) appeared to be "frozen" in their home cage hyperactivity (MGI Ref ID J:127877) the greater total activity in home cage increased eating behavior (MGI Ref ID J:127877) average food consumption was greater in mutant over control male reduced copulation (MGI Ref ID J:127877) did not produce copulation plug when mated with superovulated females nervous system phenotype absence seizures (MGI Ref ID J:127877) appeared to be "frozen" in their home cage reproductive system phenotype reduced female fertility (MGI Ref ID J:127877) four out of six female either did not produce any offspring or produced letters infrequently reduced male fertility (MGI Ref ID J:127877) reduced number of mating growth/size phenotype obese (MGI Ref ID J:127877) some mutant adult mice became obese homeostasis/metabolism phenotype altered response to anesthetics (MGI Ref ID J:127877) less sensitive to the sedative/hypnotic effects of etomidate

Gene & Allele Details

Allele Symbol		Gabrb3tm2.1Geh
Allele Name		targeted mutation 2.1, Gregg E Homanics
Common Name(s)		beta3F;
Mutation Made By		Gregg Homanics,   University of Pittsburgh
Strain of Origin		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line Name		R1
ES Cell Line Strain		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name		Gabrb3, gamma-aminobutyric acid (GABA-A) receptor, subunit beta 3
Chromosome		7
Gene Common Name(s)		A230092K12Rik; AW049585; Cp1; Gabrb-3; MGC9051; RIKEN cDNA A230092K12 gene; beta3; cleft palate 1; expressed sequence AW049585;
Molecular Note		Gabrb3tm2Geh heterozygous mice with a floxed exon 3 and neomycin cassette allele (Fneo) were bred to Tg(ACTFLPe)9205Dym actin-FLPe mice to remove the Neomycin cassette. [MGI Ref ID J:127877]

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as homozygotes.

Related Strains

Strains carrying other alleles of Gabrb3

002711

B6;129-Gabrb3tm1Geh/J

View Strains carrying other alleles of Gabrb3     (1 strain)

Additional Web Information

Cre-lox or FLP-FRT Systems

Research Applications

This mouse can be used to support research in many areas including:

Neurobiology Research
Cre-lox System (loxP-flanked Sequences)

Research Tools
Cre-lox System (loxP-flanked Sequences)

References

Selected Reference(s)

Ferguson C; Hardy SL; Werner DF; Hileman SM; Delorey TM; Homanics GE. 2007. New insight into the role of the beta3 subunit of the GABAA-R in development, behavior, body weight regulation, and anesthesia revealed by conditional gene knockout. BMC Neurosci 8:85. [PubMed: 17927825]  [MGI Ref ID J:127877]

---

Price and Supply Information

Strain Name:	B6;129-Gabrb3tm2.1Geh/J
Stock Number:	008310

 

This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.

Estimated Available for Sale Date:

Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.

View All Strains Under Development

Supply Details

Standard Supply	Under Development for Distribution Colony
Supply Notes	This strain is included in the Induced Mutant Resource Colony collection.
Licensing	See General Terms and Conditions below for Licensing and Use Restrictions

General Terms and Conditions

View JAX^® Mice & Services Conditions of Use.

Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Go to JAX^® Mice Query Form