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| Cre activity is demonstrable in the hypothalmus (arcuate, dorsomedial, lateral, and ventromedial nuclei), limbic and cortical brain regions (basolateral amygdaloid nucleus, piriform cortex, and lateral entorhinal cortex), and retrosplenial cortex. When bred with a mouse containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked genome in tissues that normally express the targeted gene. This strain has been used in virus-assisted mapping of neural inputs and may be useful in studies of neural features of feeding behaviors. | |||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System +/+ sibling x Heterozygote (Female x Male) Species laboratory mouse Generation N11+ (24-SEP-08) Donating Investigator Jeffrey Friedman, Rockefeller University, HHMI Description
Mice hemizygous for this targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. Cre activity is demonstrable in the hypothalmus (arcuate, dorsomedial (DMH), lateral (LH), and ventromedial (VMH) nuclei), limbic and cortical brain regions (basolateral amygdaloid nucleus (BLA), piriform cortex (Pir), and lateral entorhinal cortex (LEnt)), and retrosplenial cortex. When bred with a mouse containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked genome in tissues that normally express the gene. This strain has been used in virus-assisted mapping of neural inputs and may be useful in studies of neural features of feeding behaviors.Development
The targeting vector containing an IRES (internal ribosome entry site)-NLS(nuclear localization signal)-Cre and a neomycin resistance gene (flanked by frt sites) was inserted immediately 3' of the stop codon in the last exon of the gene. The vector was introduced to a 129-background embryonic stem (ES) cell line. This strain was backcrossed ten times to C57BL/6 background by the donating laboratory.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Lepr
000709 129P3/J-Leprdb-3J/J 002048 B6 x C57BLKS-m Leprdb Myo15sh2-J/J 008518 B6.129-Leprtm1Mgmj/J 008385 B6.129-Leprtm2Mgmj/J 008327 B6.129P2-Leprtm1Rck/J 000697 B6.Cg-m +/+ Leprdb/J 000699 B6.Cg-m Leprdb/+ +/J 000642 BKS.Cg-m +/+ Leprdb/J 000700 BKS.Cg-m Leprdb/+ +/J 001192 BKS.Cg-meaJ Leprdb +/+ + m/J 000707 CBA.Cg-m Leprdb/+ +/J 006654 FVB.BKS(D)-Leprdb/ChuaJ 004939 NOD/ShiLtJ-Leprdb-5J/LtJ 006846 STOCK Leprdb-9J/Jgn View Strains carrying other alleles of Lepr (14 strains)
Additional Web Information
Congenic Nomenclature
Cre-lox Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Leprtm2(cre)Rck/?
Background Not Specified
- no phenotypic analysis
- *normal* no phenotypic analysis (MGI Ref ID J:68414)
- mice generated for expression studies
This mouse can be used to support research in many areas including:
Diabetes and Obesity Research
Neurobiology Research
Behavioral and Learning Defects
Cre-lox System (Cre Recombinase expression in neural tissue)
Research Tools
Cre-lox System (Cre Recombinase Expression)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Leprtm2(cre)Rck | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Jeffrey M Friedman | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | ObRb-Cre; Obrb-IRES-Cre; | ||
| Mutation Made By | Jeffrey Friedman, Rockefeller University, HHMI | ||
| Site of Expression | Cre activity is demonstrable in the hypothalmus (arcuate, dorsomedial, lateral, and ventromedial nuclei), limbic and cortical brain regions (basolateral amygdaloid nucleus , piriform cortex, and lateral entorhinal cortex), and retrosplenial cortex. | ||
| Gene Symbol and Name | Lepr, leptin receptor | ||
| Chromosome | 4 | ||
| Gene Common Name(s) | CD295; Fa; LEPROT; Leprb; Modb1; OB-RGRP; OBR; db; diabetes; leptin receptor gene-related protein; obese-like; obl; | ||
| Molecular Note | A cre recombinase expression cassette containing an IRES domain was placed immediately downstream of the stop codon for the gene. Cre expression in the hypothalamus was confirmed by use of a virus containing a cre-dependent reporter gene. [MGI Ref ID J:68414] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, MCA, vers. 1
Generic Cre, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
DeFalco J; Tomishima M; Liu H; Zhao C; Cai X; Marth JD; Enquist L; Friedman JM. 2001. Virus-assisted mapping of neural inputs to a feeding center in the hypothalamus. Science 291(5513):2608-13. [PubMed: 11283374] [MGI Ref ID J:68414]
Additional References
Leprtm2(cre)Rck relatedPlum L; Rother E; Munzberg H; Wunderlich FT; Morgan DA; Hampel B; Shanabrough M; Janoschek R; Konner AC; Alber J; Suzuki A; Krone W; Horvath TL; Rahmouni K; Bruning JC. 2007. Enhanced leptin-stimulated pi3k activation in the CNS promotes white adipose tissue transdifferentiation. Cell Metab 6(6):431-45. [PubMed: 18054313] [MGI Ref ID J:130444]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintained as a live colony, heterozygotes may be bred. Mating System +/+ sibling x Heterozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $236.40 Female or Male Heterozyous for Leprtm2(cre)Rck *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $288.65 Heterozyous for Leprtm2(cre)Rck x Wild-type for Leprtm2(cre)Rck $288.65 Wild-type for Leprtm2(cre)Rck x Heterozyous for Leprtm2(cre)Rck
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $307.40 Female or Male Heterozyous for Leprtm2(cre)Rck *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $375.30 Heterozyous for Leprtm2(cre)Rck x Wild-type for Leprtm2(cre)Rck $375.30 Wild-type for Leprtm2(cre)Rck x Heterozyous for Leprtm2(cre)Rck
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Surgical Services
Contact Information
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Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
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Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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