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| These mice express a tau (MAPT)-sapphire green fluorescent protein (GFP) under the transcriptional control of the mouse pro-opiomelanocortin-alpha (Pomc) promoter. Immunohistochemistry for POMC in colchicine-treated animals demonstrated greater than 99% colocalization of POMC with the GFP-expressing neurons in the hypothalamic arcuate nucleus. This strain has been useful in studying the role of Pomc and leptin in food intake and may be useful in further understanding the function of the associated neurons. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6(CBA)-Tg(Pomc-MAPT/GFP*)1Rck/J (Changed: 16-MAY-08 ) Type Congenic; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Noncarrier x Hemizygote (Female x Male) 25-SEP-08 Species laboratory mouse Generation N11+N1 (18-DEC-08) Donating Investigator Jeffrey Friedman, Rockefeller University, HHMI Description
These mice express a tau (MAPT)-sapphire green fluorescent protein (GFP) under the transcriptional control of the mouse pro-opiomelanocortin-alpha (Pomc) promoter. Immunohistochemistry for POMC in colchicine-treated animals demonstrated greater than 99% colocalization of POMC with the GFP-expressing neurons in the hypothalamic arcuate nucleus. This strain has been useful in studying the role of Pomc and leptin in food intake and may be useful in further understanding the function of the associated neurons. Hemizygotes are viable and fertile and do not display any gross physical or behavioral abnormalities.Development
A transgenic BAC vector carrying Tau (MAPT)-Topaz GFP in exon 1 of a mouse pro-opiomelanocortin-alpha (Pomc) gene was introduced to B6CBAF1 embryos. The line was backcrossed ten times to C57BL/6 by the donating laboratory.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
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Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(Pomc-MAPT/GFP*)1Rck/0
involves: C57BL/6 * CBA
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:90242)
- mice display no obvious phenotypic abnormalities
This mouse can be used to support research in many areas including:
GFP relatedDiabetes and Obesity Research
Neurobiology Research
Behavioral and Learning Defects
Fluorescent protein expression in neural tissue
Research Tools
Fluorescent Proteins
MAPT relatedResearch Tools
Fluorescent Proteins
Neurobiology Research
Alzheimer's Disease
Parkinson's Disease
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Pomc-MAPT/GFP*)1Rck | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Jeffrey M Friedman | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | MCH-GFP; POMC-GFP; | ||
| Strain of Origin | (CBA x C57BL/6)F1 | ||
| Site of Expression | GFP is expressed in neurons in the hypothalamic arcuate nucleus. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Expressed Gene | MAPT, microtubule-associated protein tau, human | ||
| Promoter | Pomc, pro-opiomelanocortin-alpha, mouse, laboratory | ||
| Molecular Note | A transgenic BAC vector carrying Tau (MAPT)-Topaz GFP in exon 1 of a mouse pro-opiomelanocortin alpha (Pomc) gene was introduced to fertilized embryos. [MGI Ref ID J:90242] | ||
| Gene Symbol and Name | Tg(Pomc-MAPT/GFP*)1Rck, transgene insertion 1, Jeffrey M Friedman | ||
| Chromosome | UN | ||
| Gene Common Name(s) | MCH-GFP; POMC-GFP; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Npy-MAPT/GFP)_MCA, Melt Curve Analysis
Tg(Npy-MAPT/GFP*), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Pinto S; Roseberry AG; Liu H; Diano S; Shanabrough M; Cai X; Friedman JM; Horvath TL. 2004. Rapid rewiring of arcuate nucleus feeding circuits by leptin. Science 304(5667):110-5. [PubMed: 15064421] [MGI Ref ID J:90242]
Additional References
Tg(Pomc-MAPT/GFP*)1Rck relatedAndrews ZB; Liu ZW; Walllingford N; Erion DM; Borok E; Friedman JM; Tschop MH; Shanabrough M; Cline G; Shulman GI; Coppola A; Gao XB; Horvath TL; Diano S. 2008. UCP2 mediates ghrelin's action on NPY/AgRP neurons by lowering free radicals. Nature 454(7206):846-51. [PubMed: 18668043] [MGI Ref ID J:139938]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintained as a live colony, hemizygotes may be bred. Mating System Noncarrier x Hemizygote (Female x Male) 25-SEP-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(Pomc-MAPT/GFP*)1Rck
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Tg(Pomc-MAPT/GFP*)1Rck x Noncarrier $297.85 Noncarrier x Hemizygous for Tg(Pomc-MAPT/GFP*)1Rck
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(Pomc-MAPT/GFP*)1Rck
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Tg(Pomc-MAPT/GFP*)1Rck x Noncarrier $387.30 Noncarrier x Hemizygous for Tg(Pomc-MAPT/GFP*)1Rck
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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