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| These mice express a tau (MAPT)-sapphire green fluorescent protein (GFP) under the transcriptional control of the mouse melanocortin 4 receptor (Mc4r) promoter. This strain may be useful in studying the role of melanocortin signaling in the regulation of feeding behavior and autonomic homeostasis. | |||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6(CBA)-Tg(Mc4r-MAPT/GFP*)21Rck/J (Changed: 16-MAY-08 ) Type Congenic; Transgenic; Additional information on Genetically Engineered Mutant Mice. Mating System Hemizygote x Noncarrier (Female x Male) Species laboratory mouse Generation N11+ (24-SEP-08) Donating Investigator Jeffrey Friedman, Rockefeller University, HHMI Description
These mice express a tau (MAPT)-sapphire green fluorescent protein (GFP) under the transcriptional control of the mouse melanocortin 4 receptor (Mc4r) promoter. Central nervous system distribution of GFP-producing cells is identical to that of Mc4r mRNA in wild-type mice and nearly all GFP-producing cells coexpress melanocortin 4 mRNA. This strain may be useful in studying the role of melanocortin signaling in the regulation of feeding behavior and autonomic homeostasis. Hemizygotes are viable and fertile and do not display any gross physical or behavioral abnormalities.Development
A transgenic BAC vector carrying Tau (MAPT)-Sapphire GFP in the ATG site of a mouse melanocortin 4 receptor (Mc4r) gene was introduced to B6CBAF1 embryos. Line 21 was backcrossed ten times to C57BL/6 by the donating laboratory.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (169 strains)
006414 B6;129S4-Mc4rtm1Lowl/J
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(Mc4r-MAPT/GFP*)21Rck/?
involves: CBA * C57BL/6
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:130253)
- reporter transgene with expression detected in the brain and CNS
This mouse can be used to support research in many areas including:
GFP relatedDiabetes and Obesity Research
Neurobiology Research
Behavioral and Learning Defects
Fluorescent protein expression in neural tissue
Research Tools
Fluorescent Proteins
MAPT relatedResearch Tools
Fluorescent Proteins
Neurobiology Research
Alzheimer's Disease
Parkinson's Disease
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Mc4r-MAPT/GFP*)21Rck | ||
|---|---|---|---|
| Allele Name | transgene insertion 21, Jeffrey M Friedman | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | MC4-R/GFP; MC4R-GFP; | ||
| Strain of Origin | (CBA x C57BL/6)F1 | ||
| Site of Expression | GFP expression is detected in numerous brain regions and is identical to that of melanocortin-4 mRNA. | ||
| Expressed Gene | MAPT, microtubule-associated protein tau, human | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Mc4r, melanocortin 4 receptor, mouse, laboratory | ||
| Molecular Note | A bovine gene encoding Tau protein was fused to a gene encoding a blue-shifted variant of GFP known as Sapphire. This gene was put under the control of 50kb of Mc4r promoter sequence and 95kb of downstream sequence. Two lines were characterized with line 21 having five insertion events and line 30 having one. Reporter gene expression and endogenous Mc4r gene expression co-localized in cells of the brain as determined by in situ hybridization. This record serves as a representative record for both lines 21 and 30. [MGI Ref ID J:130253] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), MCA, vers. 2
Fluorescent Proteins (Generic GFP), STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Liu H; Kishi T; Roseberry AG; Cai X; Lee CE; Montez JM; Friedman JM; Elmquist JK. 2003. Transgenic mice expressing green fluorescent protein under the control of the melanocortin-4 receptor promoter. J Neurosci 23(18):7143-54. [PubMed: 12904474] [MGI Ref ID J:130253]
Additional References
Tg(Mc4r-MAPT/GFP*)21Rck relatedVoss-Andreae A; Murphy JG; Ellacott KL; Stuart RC; Nillni EA; Cone RD; Fan W. 2007. Role of the central melanocortin circuitry in adaptive thermogenesis of brown adipose tissue. Endocrinology 148(4):1550-60. [PubMed: 17194736] [MGI Ref ID J:129583]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintained as a live colony, hemizygotes may be bred. Mating System Hemizygote x Noncarrier (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $236.40 Female or Male Hemizygous for Tg(Mc4r-MAPT/GFP*)21Rck *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $288.65 Hemizygous for Tg(Mc4r-MAPT/GFP*)21Rck x Noncarrier $288.65 Noncarrier x Hemizygous for Tg(Mc4r-MAPT/GFP*)21Rck
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $307.40 Female or Male Hemizygous for Tg(Mc4r-MAPT/GFP*)21Rck *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $375.30 Hemizygous for Tg(Mc4r-MAPT/GFP*)21Rck x Noncarrier $375.30 Noncarrier x Hemizygous for Tg(Mc4r-MAPT/GFP*)21Rck
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Contact information
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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