Strain Name:

B6.129P2-Leprtm1Rck/J

Stock Number:

008327

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Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
These targeted mutation mice possess loxP sites on either side of exon 1 in the leptin receptor (Lepr) gene. When bred to mice bearing a Cre recombinase gene under the control of a tissue-specific promoter, the targeted gene is deleted in the tissue of interest. This strain may be useful in studies of obesity.

Description

Strain Information

Type Congenic; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Mating SystemHomozygote x Homozygote         (Female x Male)   07-JUL-14
Specieslaboratory mouse
GenerationN10+N1pN1 (17-OCT-13)
Generation Definitions
 
Donating Investigator Jeffrey Friedman,   Rockefeller University, HHMI

Description
These targeted mutation mice possess loxP sites on either side of exon 1. Homozyotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice bearing a Cre recombinase gene under the control of a tissue-specific promoter, exon 1 of the targeted gene is deleted in the tissue of interest. This strain may be useful in studies of obesity.

Development
A single loxP site was inserted 5' to exon 1 and a loxP-flanked neomycin and thymidine kinase selection cassette was inserted 3' to exon 1. The selection cassette was removed in 129P2/OlaHsd-derived E14.1 embryonic stem (ES) cells by transient Cre expression prior to the production of chimeric mice, leaving a single loxP site 3' to exon 1 in the final allele. This strain was backcrossed ten times to C57BL/6 by the donating laboratory.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of Lepr     (18 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Leptin Receptor Deficiency   (LEPR)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Leprtm1Rck/Leprtm1Rck

        involves: 129/Sv
  • normal phenotype
  • no abnormal phenotype detected   (MGI Ref ID J:72241)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Diabetes and Obesity Research

Research Tools
Cre-lox System
      loxP-flanked Sequences
Diabetes and Obesity Research
      loxP

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Leprtm1Rck
Allele Name targeted mutation 1, Jeffrey M Friedman
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) ObRflox;
Mutation Made By Jeffrey Friedman,   Rockefeller University, HHMI
Strain of Origin129/Sv
Gene Symbol and Name Lepr, leptin receptor
Chromosome 4
Gene Common Name(s) CD295; Fa; LEP-R; LEPRD; LEPROT; Leprb; Modb1; OB-R; OB-RGRP; OBR; db; diabetes; leptin receptor gene-related protein; obese-like; obl;
Molecular Note A single loxP site was inserted 5' to exon 1 and a loxP-flanked neomycin and thymidine kinase selection cassette was inserted 3' to exon 1. The selection cassette was removed in ES cells by transient Cre expression prior to the production of chimeric mice. leaving a single loxP site 3' to exon 1 in the final allele. [MGI Ref ID J:72241]

Genotyping

Genotyping Information

Genotyping Protocols

Leprtm1Rck, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Cohen P; Zhao C; Cai X; Montez JM; Rohani SC; Feinstein P; Mombaerts P; Friedman JM. 2001. Selective deletion of leptin receptor in neurons leads to obesity. J Clin Invest 108(8):1113-21. [PubMed: 11602618]  [MGI Ref ID J:72241]

Additional References

Leprtm1Rck related

Bellefontaine N; Chachlaki K; Parkash J; Vanacker C; Colledge W; d'Anglemont de Tassigny X; Garthwaite J; Bouret SG; Prevot V. 2014. Leptin-dependent neuronal NO signaling in the preoptic hypothalamus facilitates reproduction. J Clin Invest 124(6):2550-9. [PubMed: 24812663]  [MGI Ref ID J:212751]

Bingham NC; Anderson KK; Reuter AL; Stallings NR; Parker KL. 2008. Selective loss of leptin receptors in the ventromedial hypothalamic nucleus results in increased adiposity and a metabolic syndrome. Endocrinology 149(5):2138-48. [PubMed: 18258679]  [MGI Ref ID J:136006]

Cortes VA; Cautivo KM; Rong S; Garg A; Horton JD; Agarwal AK. 2014. Leptin ameliorates insulin resistance and hepatic steatosis in Agpat2-/- lipodystrophic mice independent of hepatocyte leptin receptors. J Lipid Res 55(2):276-88. [PubMed: 24293639]  [MGI Ref ID J:208288]

Goncalves GH; Li W; Garcia AV; Figueiredo MS; Bjorbaek C. 2014. Hypothalamic agouti-related peptide neurons and the central melanocortin system are crucial mediators of leptin's antidiabetic actions. Cell Rep 7(4):1093-103. [PubMed: 24813890]  [MGI Ref ID J:211797]

Harlan SM; Morgan DA; Agassandian K; Guo DF; Cassell MD; Sigmund CD; Mark AL; Rahmouni K. 2011. Ablation of the leptin receptor in the hypothalamic arcuate nucleus abrogates leptin-induced sympathetic activation. Circ Res 108(7):808-12. [PubMed: 21311043]  [MGI Ref ID J:183594]

McGaffin KR; Witham WG; Yester KA; Romano LC; O'Doherty RM; McTiernan CF; O'Donnell CP. 2011. Cardiac-specific leptin receptor deletion exacerbates ischaemic heart failure in mice. Cardiovasc Res 89(1):60-71. [PubMed: 20833647]  [MGI Ref ID J:167785]

Morioka T; Asilmaz E; Hu J; Dishinger JF; Kurpad AJ; Elias CF; Li H; Elmquist JK; Kennedy RT; Kulkarni RN. 2007. Disruption of leptin receptor expression in the pancreas directly affects beta cell growth and function in mice. J Clin Invest 117(10):2860-8. [PubMed: 17909627]  [MGI Ref ID J:127532]

Saucillo DC; Gerriets VA; Sheng J; Rathmell JC; Maciver NJ. 2014. Leptin metabolically licenses T cells for activation to link nutrition and immunity. J Immunol 192(1):136-44. [PubMed: 24273001]  [MGI Ref ID J:207097]

Scott MM; Williams KW; Rossi J; Lee CE; Elmquist JK. 2011. Leptin receptor expression in hindbrain Glp-1 neurons regulates food intake and energy balance in mice. J Clin Invest 121(6):2413-21. [PubMed: 21606595]  [MGI Ref ID J:172264]

Young CN; Morgan DA; Butler SD; Mark AL; Davisson RL. 2013. The brain subfornical organ mediates leptin-induced increases in renal sympathetic activity but not its metabolic effects. Hypertension 61(3):737-44. [PubMed: 23357182]  [MGI Ref ID J:211757]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX18

Colony Maintenance

Breeding & HusbandryWhen maintained as a live colony, homozygotes or heterozygotes may be bred.
Mating SystemHomozygote x Homozygote         (Female x Male)   07-JUL-14
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHomozygous for Leprtm1Rck  
Price per Pair (US dollars $)Pair Genotype
$464.00Homozygous for Leprtm1Rck x Homozygous for Leprtm1Rck  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHomozygous for Leprtm1Rck  
Price per Pair (US dollars $)Pair Genotype
$603.20Homozygous for Leprtm1Rck x Homozygous for Leprtm1Rck  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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