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| These targeted mutation mice possess loxP sites on either side of exon 1 in the leptin receptor (Lepr) gene. When bred to mice bearing a Cre recombinase gene under the control of a tissue-specific promoter, exon 1 of the targeted gene is deleted in the tissue of interest. This strain may be useful in studies of obesity. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Donating Investigator Jeffrey Friedman, Rockefeller University, HHMI Description
These targeted mutation mice possess loxP sites on either side of exon 1. Homozyotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice bearing a Cre recombinase gene under the control of a tissue-specific promoter, exon 1 of the targeted gene is deleted in the tissue of interest. This strain may be useful in studies of obesity.Development
A single loxP site was inserted 5' to exon 1 and a loxP-flanked neomycin and thymidine kinase selection cassette was inserted 3' to exon 1. The selection cassette was removed in 129P2/OlaHsd-derived E14.1 embryonic stem (ES) cells by transient Cre expression prior to the production of chimeric mice, leaving a single loxP site 3' to exon 1 in the final allele. This strain was backcrossed ten times to C57BL/6 by the donating laboratory.
Related Strains
Strains carrying other alleles of Lepr
000709 129P3/J-Leprdb-3J/J 002048 B6 x C57BLKS-Dock7m Leprdb Myo15sh2-J/J 017527 B6.129(FVB)-Leprtm5Mgmj/J 008518 B6.129-Leprtm1Mgmj/J 008320 B6.129-Leprtm2(cre)Rck/J 008385 B6.129-Leprtm2Mgmj/J 000697 B6.BKS(D)-Leprdb/J 000699 B6.Cg-Dock7m Leprdb/+ +/J 000642 BKS.Cg-Dock7m +/+ Leprdb/J 000700 BKS.Cg-Dock7m Leprdb/+ +/J 008340 BKS.Cg-Leprdb Nos3tm1Unc/RhrsJ 001192 BKS.Cg-meaJ Leprdb +/+ + Dock7m/J 000707 CBA.Cg-Dock7m Leprdb/+ +/J 012666 D2.BKS(D)-Leprdb/J 006654 FVB.BKS(D)-Leprdb/ChuaJ 004939 NOD/ShiLtJ-Leprdb-5J/LtJ 006846 STOCK Leprdb-9J/Jgn 018989 STOCK Leprtm1Jke/J View Strains carrying other alleles of Lepr (18 strains)
Phenotype
Phenotype Information
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Leptin Receptor Deficiency (LEPR)
This mouse can be used to support research in many areas including:
Research Tools
Cre-lox System
loxP-flanked Sequences
Diabetes and Obesity Research
loxP
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Leprtm1Rck | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Jeffrey M Friedman | ||
| Allele Type | Targeted (Floxed/Frt) | ||
| Common Name(s) | ObRflox; | ||
| Mutation Made By | Jeffrey Friedman, Rockefeller University, HHMI | ||
| Strain of Origin | 129/Sv | ||
| Gene Symbol and Name | Lepr, leptin receptor | ||
| Chromosome | 4 | ||
| Gene Common Name(s) | CD295; LEP-R; LEPRD; LEPROT; Leprb; Modb1; OB-R; OB-RGRP; OBR; db; diabetes; leptin receptor gene-related protein; obese-like; obl; | ||
| Molecular Note | A single loxP site was inserted 5' to exon 1 and a loxP-flanked neomycin and thymidine kinase selection cassette was inserted 3' to exon 1. The selection cassette was removed in ES cells by transient Cre expression prior to the production of chimeric mice. leaving a single loxP site 3' to exon 1 in the final allele. [MGI Ref ID J:72241] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Leprtm1Rck, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Cohen P; Zhao C; Cai X; Montez JM; Rohani SC; Feinstein P; Mombaerts P; Friedman JM. 2001. Selective deletion of leptin receptor in neurons leads to obesity. J Clin Invest 108(8):1113-21. [PubMed: 11602618] [MGI Ref ID J:72241]
Additional References
Leprtm1Rck relatedBingham NC; Anderson KK; Reuter AL; Stallings NR; Parker KL. 2008. Selective loss of leptin receptors in the ventromedial hypothalamic nucleus results in increased adiposity and a metabolic syndrome. Endocrinology 149(5):2138-48. [PubMed: 18258679] [MGI Ref ID J:136006]
Harlan SM; Morgan DA; Agassandian K; Guo DF; Cassell MD; Sigmund CD; Mark AL; Rahmouni K. 2011. Ablation of the leptin receptor in the hypothalamic arcuate nucleus abrogates leptin-induced sympathetic activation. Circ Res 108(7):808-12. [PubMed: 21311043] [MGI Ref ID J:183594]
McGaffin KR; Witham WG; Yester KA; Romano LC; O'Doherty RM; McTiernan CF; O'Donnell CP. 2011. Cardiac-specific leptin receptor deletion exacerbates ischaemic heart failure in mice. Cardiovasc Res 89(1):60-71. [PubMed: 20833647] [MGI Ref ID J:167785]
Morioka T; Asilmaz E; Hu J; Dishinger JF; Kurpad AJ; Elias CF; Li H; Elmquist JK; Kennedy RT; Kulkarni RN. 2007. Disruption of leptin receptor expression in the pancreas directly affects beta cell growth and function in mice. J Clin Invest 117(10):2860-8. [PubMed: 17909627] [MGI Ref ID J:127532]
Scott MM; Williams KW; Rossi J; Lee CE; Elmquist JK. 2011. Leptin receptor expression in hindbrain Glp-1 neurons regulates food intake and energy balance in mice. J Clin Invest 121(6):2413-21. [PubMed: 21606595] [MGI Ref ID J:172264]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintained as a live colony, homozygotes or heterozygotes may be bred.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2250.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2925.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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