Strain Name:

CByJ.B6(Cg)-Rag2tm1Cgn/J

Stock Number:

008338

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
These RAG-2fl mice harbor loxP sites flanking the entire coding region of the Rag2 (recombination activating gene 2) locus. When bred to mice that express Cre recombinase, the resulting offspring will have exon 3 deleted in the cre-expressing tissue(s). These RAG-2fl may be useful in generating conditional mutations for studying the role of RAG-2 in B and T cell development (including cancer and toxicology research as a xenograft/transplant host), T and B cell receptor (V(D)J) recombination, hematopoiesis, hematology, immunology, and inflammation research.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
GenerationN6F1pN1
Generation Definitions
 
Donating Investigator IMR Colony,   The Jackson Laboratory

Description
Mice homozygous for the RAG-2fl allele are viable and fertile, with loxP sites flanking exon 3 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region (coding for the entire RAG-2 protein) deleted in the cre-expressing tissue(s). These RAG-2fl mice may be useful in generating conditional mutations for studying the role of RAG-2 in B and T cell development (including cancer and toxicology research as a xenograft/transplant host), T and B cell receptor (V(D)J) recombination, hematopoiesis, hematology, immunology, and inflammation research.

For example, when bred to a strain with inducible Cre recombinase expression in liver and lymphocytes (see Stock No. 003556), this mutant mouse strain may be useful in studies of B and T cell development.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development
A targeting vector was designed to insert a loxP-flanked neomycin resistance gene on one side, and a third loxP site on the other side of exon 3 of the targeted gene. This construct was electroporated into C57BL/6-derived Bruce-4 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a cre-expressing vector to remove the selection cassette. ES cells in which the neomycin resistance cassette was deleted (leaving a single loxP site upstream, and a single loxP downstream of exon 3) were injected into recipient blastocysts. Chimeric males were bred with C57BL/6 females to generate RAG-2fl mice. These RAG-2fl mice were bred with transgenic mice on mixed genetic background, and then bred with mutant mice on a C57BL/6 genetic background for 8 generations. The donating investigator reports that both the transgene and other mutant gene were bred out of these RAG-2fl mice prior to arrival at The Jackson Laboratory (as Stock No. 008309). Upon arrival, some mice were backcrossed to BALB/cByJ (Stock No. 001026) using a marker-assisted approach to generate this congenic strain (Stock No. 008338).

Control Information

  Control
   001026 BALB/cByJ
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Rag2tm1Cgn allele
008309   C57BL/6-Rag2tm1Cgn/J
View Strains carrying   Rag2tm1Cgn     (1 strain)

View Strains carrying other alleles of Rag2     (11 strains)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Combined Cellular and Humoral Immune Defects with Granulomas; CCHIDG   (RAG2)
Omenn Syndrome   (RAG2)
Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-Negative, B Cell-Negative, Nk Cell-Positive   (RAG2)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Rag2tm1Cgn/Rag2tm1Cgn

        C57BL/6-Rag2tm1Cgn
  • normal phenotype
  • no abnormal phenotype detected
    • B cell lymphopoiesis in the bone marrow is normal   (MGI Ref ID J:72214)
    • the number of B cells found in circulation is comparable to that of wild-type mice   (MGI Ref ID J:72214)

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

Rag2tm1Cgn/Rag2tm1.1Cgn Tg(Mx1-cre)1Cgn/0

        involves: C57BL/6 * CBA   (conditional)
  • immune system phenotype
  • abnormal B cell activation
    • peripheral B cells have an activated phenotype in mice that have Cre expression induced at birth   (MGI Ref ID J:72214)
    • the activated phenotype include larger cell size and increased expression of CD25, CD69, CD86 and MHC-II when analyzed at 8 weeks of age   (MGI Ref ID J:72214)
    • B cells do not have this activated phenotype when Cre is induced in mice 8-10 weeks of age   (MGI Ref ID J:72214)
  • absent immature B cells
    • the IgM+HSAhigh immature B cell compartment in the spleen is absent two weeks after induction of Cre expression in mice 8-10 weeks old   (MGI Ref ID J:72214)
    • absent pre-B cells
      • pre-B cells are absent in the bone marrow of mice two weeks after induction of Cre expression in mice 8-10 weeks old   (MGI Ref ID J:72214)
  • arrested B cell differentiation
    • B cell development is arrested at the pro-B cell stage two weeks after induction of Cre expression in mice 8-10 weeks old   (MGI Ref ID J:72214)
  • arrested T cell differentiation
    • T cell development is arrested at the pro-T cell stage two weeks after induction of Cre expression in mice 8-10 weeks old   (MGI Ref ID J:72214)
  • decreased mature B cell number
    • the number of mature B cells in the bone marrow drop by half within two weeks of induction of Cre expression in mice 8-10 weeks old and then decline slowly over the next year with a half-life of 156 days   (MGI Ref ID J:72214)
    • the number of mature B cells in the spleen is reduced by 67% two weeks after Cre induction   (MGI Ref ID J:72214)
    • mature B cell numbers are reduced to 32% in the spleen 17 weeks after Cre induction and remain at this level for at least one year   (MGI Ref ID J:72214)
    • mature B cell numbers in lymph nodes drop to 58% of normal 10 weeks after Cre induction, and continue to drop to 11% of normal 17 weeks after Cre induction   (MGI Ref ID J:72214)
    • mature B cell numbers remain at 11% of normal between 17 and 54 weeks after induction of Cre expression   (MGI Ref ID J:72214)
    • when Cre is induced in neonates, mature B cells numbers are 10- to 30- fold below normal   (MGI Ref ID J:72214)
    • decreased follicular B cell number
      • the number of follicular B cells in the spleen decline slowly after induction of Cre expression in mice 8-10 weeks old with a half-life of 134 days   (MGI Ref ID J:72214)
    • decreased marginal zone B cell number
      • marginal zone B cells are decreased in number by 75% when Cre-induction occurs at birth   (MGI Ref ID J:72214)
      • the number of marginal zone B cells increases slightly with age but remains well below normal levels   (MGI Ref ID J:72214)
      • marginal zone B cell numbers are normal when Cre-induction occurs in mice 8 to 10 weeks of age   (MGI Ref ID J:72214)
  • increased plasma cell number
    • the total number of IgM secreting plasma cells found in the spleen is 3- to 4- fold larger in mice where Cre induction is induced at birth than in controls   (MGI Ref ID J:72214)
  • hematopoietic system phenotype
  • abnormal B cell activation
    • peripheral B cells have an activated phenotype in mice that have Cre expression induced at birth   (MGI Ref ID J:72214)
    • the activated phenotype include larger cell size and increased expression of CD25, CD69, CD86 and MHC-II when analyzed at 8 weeks of age   (MGI Ref ID J:72214)
    • B cells do not have this activated phenotype when Cre is induced in mice 8-10 weeks of age   (MGI Ref ID J:72214)
  • absent immature B cells
    • the IgM+HSAhigh immature B cell compartment in the spleen is absent two weeks after induction of Cre expression in mice 8-10 weeks old   (MGI Ref ID J:72214)
    • absent pre-B cells
      • pre-B cells are absent in the bone marrow of mice two weeks after induction of Cre expression in mice 8-10 weeks old   (MGI Ref ID J:72214)
  • arrested B cell differentiation
    • B cell development is arrested at the pro-B cell stage two weeks after induction of Cre expression in mice 8-10 weeks old   (MGI Ref ID J:72214)
  • arrested T cell differentiation
    • T cell development is arrested at the pro-T cell stage two weeks after induction of Cre expression in mice 8-10 weeks old   (MGI Ref ID J:72214)
  • decreased mature B cell number
    • the number of mature B cells in the bone marrow drop by half within two weeks of induction of Cre expression in mice 8-10 weeks old and then decline slowly over the next year with a half-life of 156 days   (MGI Ref ID J:72214)
    • the number of mature B cells in the spleen is reduced by 67% two weeks after Cre induction   (MGI Ref ID J:72214)
    • mature B cell numbers are reduced to 32% in the spleen 17 weeks after Cre induction and remain at this level for at least one year   (MGI Ref ID J:72214)
    • mature B cell numbers in lymph nodes drop to 58% of normal 10 weeks after Cre induction, and continue to drop to 11% of normal 17 weeks after Cre induction   (MGI Ref ID J:72214)
    • mature B cell numbers remain at 11% of normal between 17 and 54 weeks after induction of Cre expression   (MGI Ref ID J:72214)
    • when Cre is induced in neonates, mature B cells numbers are 10- to 30- fold below normal   (MGI Ref ID J:72214)
    • decreased follicular B cell number
      • the number of follicular B cells in the spleen decline slowly after induction of Cre expression in mice 8-10 weeks old with a half-life of 134 days   (MGI Ref ID J:72214)
    • decreased marginal zone B cell number
      • marginal zone B cells are decreased in number by 75% when Cre-induction occurs at birth   (MGI Ref ID J:72214)
      • the number of marginal zone B cells increases slightly with age but remains well below normal levels   (MGI Ref ID J:72214)
      • marginal zone B cell numbers are normal when Cre-induction occurs in mice 8 to 10 weeks of age   (MGI Ref ID J:72214)
  • increased plasma cell number
    • the total number of IgM secreting plasma cells found in the spleen is 3- to 4- fold larger in mice where Cre induction is induced at birth than in controls   (MGI Ref ID J:72214)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Developmental Biology Research
Internal/Organ Defects
      hematopoietic defects
Lymphoid Tissue Defects
      hematopoietic defects

Hematological Research
Hematopoietic Defects
Immunological Defects
      B and T cell deficiency

Immunology, Inflammation and Autoimmunity Research
Autoimmunity
      B and T cell deficiency
      B cell deficiency
Immunodeficiency
      B and T cell deficiency
      B cell defects
      B cell deficiency
      T cell deficiency
Inflammation
      B and T cell deficiency
Lymphoid Tissue Defects
      B and T cell deficiency
      hematopoietic development
T Cell Receptor Signaling Defects
      B and T cell deficiency
      B and T cell deficiency, xenograft/transplant host

Internal/Organ Research
Lymphoid Tissue Defects
      B and T cell deficiency
      T cell deficiency
Thymus Defects
      B and T cell deficient

Research Tools
Cancer Research
      B and T cell deficiency, xenograft/transplant host
      B cell deficiency
      T cell deficiency
Cre-lox System
      loxP-flanked Sequences
Hematological Research
Immunology, Inflammation and Autoimmunity Research
      B and T cell deficiency
      B cell deficiency
      T Cell Receptor Deficiency
      T cell deficiency
      T cell deficiency, xenograft/transplant host
Toxicology Research
      B and T cell deficiency, xenograft transplant host
      xenograft/transplant host

Virology Research
B and T Cell Deficiency

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Rag2tm1Cgn
Allele Name targeted mutation 1, University of Cologne
Allele Type Targeted (Floxed/Frt)
Common Name(s) RAG-2fl;
Mutation Made By Klaus Rajewsky,   Max-Delbruck-Ctr. for Molecular Medicine
Strain of OriginB6.Cg-Thy1
ES Cell Line NameBruce 4
ES Cell Line StrainB6.Cg-Thy1
Gene Symbol and Name Rag2, recombination activating gene 2
Chromosome 2
Gene Common Name(s) RAG-2; Rag-2;
Molecular Note LoxP sites were inserted to flank the coding region of the gene. The inserted sites had no effect on the normal function of the gene. [MGI Ref ID J:72214]

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Hao Z; Rajewsky K. 2001. Homeostasis of peripheral b cells in the absence of b cell influx from the bone marrow. J Exp Med 194(8):1151-64. [PubMed: 11602643]  [MGI Ref ID J:72214]

Additional References

Rag2tm1Cgn related

Abe H; Kimura A; Tsuruta S; Fukaya T; Sakaguchi R; Morita R; Sekiya T; Shichita T; Chayama K; Fujii-Kuriyama Y; Yoshimura A. 2014. Aryl hydrocarbon receptor plays protective roles in ConA-induced hepatic injury by both suppressing IFN-gamma expression and inducing IL-22. Int Immunol 26(3):129-37. [PubMed: 24150244]  [MGI Ref ID J:207020]

Alcantara Llaguno S; Chen J; Kwon CH; Jackson EL; Li Y; Burns DK; Alvarez-Buylla A; Parada LF. 2009. Malignant astrocytomas originate from neural stem/progenitor cells in a somatic tumor suppressor mouse model. Cancer Cell 15(1):45-56. [PubMed: 19111880]  [MGI Ref ID J:143505]

Bourgeois C; Hao Z; Rajewsky K; Potocnik AJ; Stockinger B. 2008. Ablation of thymic export causes accelerated decay of naive CD4 T cells in the periphery because of activation by environmental antigen. Proc Natl Acad Sci U S A 105(25):8691-6. [PubMed: 18562288]  [MGI Ref ID J:143955]

Casola S; Cattoretti G; Uyttersprot N; Koralov SB; Segal J; Hao Z; Waisman A; Egert A; Ghitza D; Rajewsky K. 2006. Tracking germinal center B cells expressing germ-line immunoglobulin gamma1 transcripts by conditional gene targeting. Proc Natl Acad Sci U S A 103(19):7396-401. [PubMed: 16651521]  [MGI Ref ID J:109583]

Cheung AF; Dupage MJ; Dong HK; Chen J; Jacks T. 2008. Regulated expression of a tumor-associated antigen reveals multiple levels of T-cell tolerance in a mouse model of lung cancer. Cancer Res 68(22):9459-68. [PubMed: 19010921]  [MGI Ref ID J:141383]

Do JS; Visperas A; Oh K; Stohlman SA; Min B. 2012. Memory CD4 T cells induce selective expression of IL-27 in CD8+ dendritic cells and regulate homeostatic naive T cell proliferation. J Immunol 188(1):230-7. [PubMed: 22116827]  [MGI Ref ID J:180589]

Fritz JH; Rojas OL; Simard N; McCarthy DD; Hapfelmeier S; Rubino S; Robertson SJ; Larijani M; Gosselin J; Ivanov II; Martin A; Casellas R; Philpott DJ; Girardin SE; McCoy KD; Macpherson AJ; Paige CJ; Gommerman JL. 2012. Acquisition of a multifunctional IgA+ plasma cell phenotype in the gut. Nature 481(7380):199-203. [PubMed: 22158124]  [MGI Ref ID J:180510]

Guo S; Cobb D; Smeltz RB. 2009. T-bet inhibits the in vivo differentiation of parasite-specific CD4+ Th17 cells in a T cell-intrinsic manner. J Immunol 182(10):6179-86. [PubMed: 19414771]  [MGI Ref ID J:148240]

Hale JS; Ames KT; Boursalian TE; Fink PJ. 2010. Cutting Edge: Rag deletion in peripheral T cells blocks TCR revision. J Immunol 184(11):5964-8. [PubMed: 20435935]  [MGI Ref ID J:161222]

Hendricks DW; Fink PJ. 2009. Uneven colonization of the lymphoid periphery by T cells that undergo early TCR{alpha} rearrangements. J Immunol 182(7):4267-74. [PubMed: 19299725]  [MGI Ref ID J:147122]

Inoue M; Moriwaki Y; Arikawa T; Chen YH; Oh YJ; Oliver T; Shinohara ML. 2011. Cutting edge: critical role of intracellular osteopontin in antifungal innate immune responses. J Immunol 186(1):19-23. [PubMed: 21135164]  [MGI Ref ID J:168800]

Jiang Q; Huang J; Li WQ; Cavinato T; Keller JR; Durum SK. 2007. Role of the intracellular domain of IL-7 receptor in T cell development. J Immunol 178(1):228-34. [PubMed: 17182559]  [MGI Ref ID J:141936]

Kim S; Prout M; Ramshaw H; Lopez AF; LeGros G; Min B. 2010. Cutting edge: basophils are transiently recruited into the draining lymph nodes during helminth infection via IL-3, but infection-induced Th2 immunity can develop without basophil lymph node recruitment or IL-3. J Immunol 184(3):1143-7. [PubMed: 20038645]  [MGI Ref ID J:159532]

Krishnamoorthy G; Saxena A; Mars LT; Domingues HS; Mentele R; Ben-Nun A; Lassmann H; Dornmair K; Kurschus FC; Liblau RS; Wekerle H. 2009. Myelin-specific T cells also recognize neuronal autoantigen in a transgenic mouse model of multiple sclerosis. Nat Med 15(6):626-32. [PubMed: 19483694]  [MGI Ref ID J:151335]

Kruschinski A; Moosmann A; Poschke I; Norell H; Chmielewski M; Seliger B; Kiessling R; Blankenstein T; Abken H; Charo J. 2008. Engineering antigen-specific primary human NK cells against HER-2 positive carcinomas. Proc Natl Acad Sci U S A 105(45):17481-6. [PubMed: 18987320]  [MGI Ref ID J:143195]

Mazzucchelli R; Hixon JA; Spolski R; Chen X; Li WQ; Hall VL; Willette-Brown J; Hurwitz AA; Leonard WJ; Durum SK. 2008. Development of regulatory T cells requires IL-7Ralpha stimulation by IL-7 or TSLP. Blood 112(8):3283-92. [PubMed: 18664628]  [MGI Ref ID J:140217]

Nemeth K; Leelahavanichkul A; Yuen PS; Mayer B; Parmelee A; Doi K; Robey PG; Leelahavanichkul K; Koller BH; Brown JM; Hu X; Jelinek I; Star RA; Mezey E. 2009. Bone marrow stromal cells attenuate sepsis via prostaglandin E(2)-dependent reprogramming of host macrophages to increase their interleukin-10 production. Nat Med 15(1):42-9. [PubMed: 19098906]  [MGI Ref ID J:146566]

Nurieva RI; Chung Y; Martinez GJ; Yang XO; Tanaka S; Matskevitch TD; Wang YH; Dong C. 2009. Bcl6 mediates the development of T follicular helper cells. Science 325(5943):1001-5. [PubMed: 19628815]  [MGI Ref ID J:151785]

Petersen BC; Budelsky AL; Baptist AP; Schaller MA; Lukacs NW. 2012. Interleukin-25 induces type 2 cytokine production in a steroid-resistant interleukin-17RB+ myeloid population that exacerbates asthmatic pathology. Nat Med 18(5):751-8. [PubMed: 22543263]  [MGI Ref ID J:185138]

Poppensieker K; Otte DM; Schurmann B; Limmer A; Dresing P; Drews E; Schumak B; Klotz L; Raasch J; Mildner A; Waisman A; Scheu S; Knolle P; Forster I; Prinz M; Maier W; Zimmer A; Alferink J. 2012. CC chemokine receptor 4 is required for experimental autoimmune encephalomyelitis by regulating GM-CSF and IL-23 production in dendritic cells. Proc Natl Acad Sci U S A 109(10):3897-902. [PubMed: 22355103]  [MGI Ref ID J:182145]

Ribot JC; deBarros A; Pang DJ; Neves JF; Peperzak V; Roberts SJ; Girardi M; Borst J; Hayday AC; Pennington DJ; Silva-Santos B. 2009. CD27 is a thymic determinant of the balance between interferon-gamma- and interleukin 17-producing gammadelta T cell subsets. Nat Immunol 10(4):427-36. [PubMed: 19270712]  [MGI Ref ID J:148001]

Riol-Blanco L; Lazarevic V; Awasthi A; Mitsdoerffer M; Wilson BS; Croxford A; Waisman A; Kuchroo VK; Glimcher LH; Oukka M. 2010. IL-23 receptor regulates unconventional IL-17-producing T cells that control bacterial infections. J Immunol 184(4):1710-20. [PubMed: 20083652]  [MGI Ref ID J:159487]

Strainic MG; Shevach EM; An F; Lin F; Medof ME. 2012. Absence of signaling into CD4(+) cells via C3aR and C5aR enables autoinductive TGF-beta1 signaling and induction of Foxp3(+) regulatory T cells. Nat Immunol 14(2):162-71. [PubMed: 23263555]  [MGI Ref ID J:192613]

Wang JH; Gostissa M; Yan CT; Goff P; Hickernell T; Hansen E; Difilippantonio S; Wesemann DR; Zarrin AA; Rajewsky K; Nussenzweig A; Alt FW. 2009. Mechanisms promoting translocations in editing and switching peripheral B cells. Nature 460(7252):231-6. [PubMed: 19587764]  [MGI Ref ID J:150339]

Xiao W; Hong H; Kawakami Y; Kato Y; Wu D; Yasudo H; Kimura A; Kubagawa H; Bertoli LF; Davis RS; Chau LA; Madrenas J; Hsia CC; Xenocostas A; Kipps TJ; Hennighausen L; Iwama A; Nakauchi H; Kawakami T. 2009. Tumor suppression by phospholipase C-beta3 via SHP-1-mediated dephosphorylation of Stat5. Cancer Cell 16(2):161-71. [PubMed: 19647226]  [MGI Ref ID J:151972]

Young NP; Crowley D; Jacks T. 2011. Uncoupling Cancer Mutations Reveals Critical Timing of p53 Loss in Sarcomagenesis. Cancer Res 71(11):4040-7. [PubMed: 21512139]  [MGI Ref ID J:172206]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryMutant mice were bred to BALB/cByJ (Stock No. 001026) using a marker-assisted approach to establish this congenic strain. When maintaining the live congenic colony, homozygous mice may be bred together.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2450.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3185.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   001026 BALB/cByJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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