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| The TetTag mouse is a bi-transgenic mutant that has tetracycline (or tetracycline analog) inducible expression of beta-galactosidase in activated neurons. This strain provides a tool that allows the inducible stable labeling of activated neurons. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System See Colony Maintenance Species laboratory mouse Generation N?+N1 (01-JUL-09) Donating Investigator Mark Mayford, The Scripps Research Institute Description
The TetTag mouse is a bi-transgenic mutant that has tetracycline (or tetracycline analog) inducible expression of beta-galactosidase in activated neurons. Two independently generated transgenic strains were crossed to produce this bi-transgenic TetTag strain. In the first transgenic construct, the tetracycline-controlled transactivator (rTA) protein and a two hour half-life Green Fluorescent Protein (shEGFP) are expressed under the direction of the fos, FBJ osteosarcoma oncogene, minimal promoter. The second transgenic construct expresses a nuclear-localizing beta-galactosidase gene and the doxycycline insensitive tetracycline regulated transactivator (containing point mutation, H100Y), under the control of the tetO, tetracycline-responsive regulatory element. In the absence of tetracycline or a tetracycline analog (such as doxycycline), selective expression of beta-galactosidase is observed in activated neurons. Doxycycline administration prevents expression beta-galactosidase in neurons. However, neurons that were induced to express beta-galactosidase during the absence of doxycycline administration will continue to express beta-galactosidase via the doxycycline insensitive mutant tTAH100Y. Mice hemizygous for each transgene are viable and fertile. The strain is maintained with doxycycline adminstration to prevent neuronal beta-galactosidase expression. The Donating Investigator has not attempted to make these mice homozygous for the transgenes. This strain provides a tool that allows the inducible stable labeling of activated neurons.Development
To produce this bi-transgenic line, two independently generated single transgenic lines were crossed.The first transgenic line was generated by co-injection of a construct containing the reverse tetracycline regulated transactivator (rtTA), SV40 polyadenylation signal sequence and the fos, FBJ osteosarcoma oncogene, minimal promoter and a second construct containing a two hour half-life Green Fluorescent Protein under the control of the fos, FBJ osteosarcoma oncogene, minimal promoter, into fertilized B6D2F2 mouse eggs. These co-injected transgenes segregated as a single transgene.
The second transgenic line was generated using a construct including sequence encoding tau-LacZ and the doxycycline insensitive tetracycline regulated transactivator (containing point mutation, H100Y), under the control of the tetO, tetracycline-responsive regulatory element. This construct was injected into fertilized B6D2F2 mouse eggs.
The two independent transgenic lines were then crossed to produce this bi-transgenic strain. The mice were then backcrossed to C57BL/6J for 3 generations.
Control Information
| Control | ||
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| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
006100 B10.Cg-H2k Tg(NFkB/Fos-luc)26Rinc/J 002099 B6.129X1-Fostm1Pa/J 002293 B6;129X1-Fostm1Pa/J
007004 B6.Cg-Tg(Camk2a-tTA)1Mmay/DboJ 003767 B6.Cg-Tg(Eno2tTA)5021Nes/J 003763 B6.Cg-Tg(Eno2tTA)5030Nes/J 005964 B6.Cg-Tg(GFAP-tTA)110Pop/J 002618 B6.Cg-Tg(MMTVtTA)1Mam/J 008284 B6.Cg-Tg(Scg2-tTA)1Jt/J 006361 B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J 003563 B6.Cg-Tg(tTALap)5Bjd/J 002709 B6;C3-Tg(TettTALuc)1Dgs/J 003010 B6;CBA-Tg(Camk2a-tTA)1Mmay/J 010573 B6;SJL-Tg(Prl-tTA)6-5Jek/J 008082 B6;SJL-Tg(Tagln-tTA)1Mrab Tg(tetO-Mcpt1)1Mrab/J 005625 FVB-Tg(Pcp2-tTA)3Horr/J 003170 FVB.Cg-Tg(Myh6-tTA)6Smbf/J 006209 FVB.Cg-Tg(Tal1-tTA)19Dgt/J 005942 FVB/N-Tg(Pf4-tTA/VP16)42Kra/J 004937 NOD.Cg-Tg(Ins2-tTA)1Doi/DoiJ 006999 STOCK Dbttm1Geh Tg(tTALap)5Bjd Tg(tetO-DBT)A1Geh/J 009606 STOCK Tg(Six2-EGFP/cre)1Amc/J 003271 STOCK Tg(tTAhCMV)3Bjd/J 003275 STOCK Tg(tetL)1Bjd/J 003274 STOCK Tg(tetNZL)2Bjd/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Tet Expression Systems
Phenotype
Phenotype Information
assigned by genotype
Tg(Fos-tTA,Fos-EGFP*)1Mmay/0 Tg(tetO-lacZ,tTA*)1Mmay/0
involves: C57BL/6 * DBA/2
- no phenotypic analysis
- *normal* no phenotypic analysis (MGI Ref ID J:125221)
- transgenic mice are used only for lineage tracing; expression data, but no phenotypic analysis are provided
This mouse can be used to support research in many areas including:
GFP relatedNeurobiology Research
lacZ expression in neural tissue
Fluorescent protein expression in neural tissue
Tet Expression System
tTA/rtTA Expressing Strains
tTA/rtTA Responsive Strains
Research Tools
lacZ Expression
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers: neurons
Neurobiology Research
Tetop Tet System
cell marker
lacZ relatedResearch Tools
Fluorescent Proteins
lacZ Expression
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Fos-tTA,Fos-EGFP*)1Mmay | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Mark Mayford | ||
| Allele Type | Transgenic (random, expressed) | ||
| Strain of Origin | (C57BL/6 x DBA/2)F2 | ||
| Site of Expression | activated neurons in the basolateral amygdala | ||
| Expressed Gene | tTA, tetracycline-controlled transactivator, E. coli | ||
| The tetracycline-resistance gene (TetR), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). TetR was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). The tetracycline-inhibitable transcription factor is a component of a bigenic system that allows doxycycline (a tetracycline analog) regulatable expression of genes that are under the direction of the tetracycline responsive promoter (TetOp)promoter. | |||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Fos, FBJ osteosarcoma oncogene, mouse, laboratory | ||
| Molecular Note | A construct containing the Fos (FBJ osteosarcoma oncogene, minimal promoter) driving the tetracycline regulated transactivator (tTA) sequence, the SV40 polyadenylation signal sequence and a second construct containing a two hour half-life Green Fluorescent Protein (shEGFP) under the control of the Fos minimal promoter, were co-injected into fertilized B6D2F2 mouse eggs. These co-injected transgenes segregate as a single transgene. [MGI Ref ID J:125221] | ||
| Allele Symbol | Tg(tetO-lacZ,tTA*)1Mmay | ||
| Allele Name | transgene insertion 1, Mark Mayford | ||
| Allele Type | Transgenic (Reporter) | ||
| Strain of Origin | (C57BL/6 x DBA/2)F2 | ||
| Site of Expression | activated neurons in the basolateral amygdala | ||
| Expressed Gene | tTA, tetracycline-controlled transactivator, E. coli | ||
| The tetracycline-resistance gene (TetR), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). TetR was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). The tetracycline-inhibitable transcription factor is a component of a bigenic system that allows doxycycline (a tetracycline analog) regulatable expression of genes that are under the direction of the tetracycline responsive promoter (TetOp)promoter. | |||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Promoter | tetO, tet operator, | ||
| Molecular Note | A construct including the sequence encoding tau-LacZ and a doxycycline-insensitive tetracycline regulated transactivator (containing point mutation, H100Y), under the control of a bi-cistronic tetO promoter (tetracycline-responsive regulatory element) was injected into fertilized eggs. [MGI Ref ID J:125221] | ||
| Gene Symbol and Name | Tg(tetO-lacZ,tTA*)1Mmay, transgene insertion 1, Mark Mayford | ||
| Chromosome | UN | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Standard PCR
Generic LacZ Melt Curve Analysis, Melt Curve Analysis
Generic LacZ, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Reijmers LG; Perkins BL; Matsuo N; Mayford M. 2007. Localization of a stable neural correlate of associative memory. Science 317(5842):1230-3. [PubMed: 17761885] [MGI Ref ID J:125221]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are bred hemizygous for each transgene. The strain is maintained with doxycycline adminstration to prevent neuronal beta-galactosidase expression. The donating investigator has not attempted to make this strain homozygous for the transgenes. Mating System See above Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(Fos-tTA,Fos-EGFP*)1Mmay, Hemizygous for Tg(tetO-lacZ,tTA*)1Mmay
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Tg(Fos-tTA,Fos-EGFP*)1Mmay, Hemizygous for Tg(tetO-lacZ,tTA*)1Mmay x Noncarrier, Noncarrier $297.85 Noncarrier, Noncarrier x Hemizygous for Tg(Fos-tTA,Fos-EGFP*)1Mmay, Hemizygous for Tg(tetO-lacZ,tTA*)1Mmay
Additional Supply Details
| Pricing for International shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(Fos-tTA,Fos-EGFP*)1Mmay, Hemizygous for Tg(tetO-lacZ,tTA*)1Mmay
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Tg(Fos-tTA,Fos-EGFP*)1Mmay, Hemizygous for Tg(tetO-lacZ,tTA*)1Mmay x Noncarrier, Noncarrier $387.30 Noncarrier, Noncarrier x Hemizygous for Tg(Fos-tTA,Fos-EGFP*)1Mmay, Hemizygous for Tg(tetO-lacZ,tTA*)1Mmay
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
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