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| The TetTag mouse is a bi-transgenic mutant that has tetracycline (or tetracycline analog) inducible expression of beta-galactosidase in activated neurons. This strain provides a tool that allows the inducible stable labeling of activated neurons. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System See Colony Maintenance under the Health & husbandry tab (Female x Male) 06-FEB-09 Species laboratory mouse Generation N?+N2F6 (29-JUN-11)
Generation DefinitionsDonating Investigator Dr. Mark Mayford, The Scripps Research Institute Description
The TetTag mouse is a bi-transgenic mutant that has tetracycline (or tetracycline analog) inducible expression of beta-galactosidase in activated neurons. Two independently generated transgenic strains were crossed to produce this bi-transgenic TetTag strain. In the first transgenic construct, the tetracycline-controlled transactivator (tTA) protein and a two hour half-life Green Fluorescent Protein (shEGFP) are expressed under the direction of the fos, FBJ osteosarcoma oncogene, minimal promoter. The second transgenic construct expresses a nuclear-localizing beta-galactosidase gene and the doxycycline insensitive tetracycline regulated transactivator (containing point mutation, H100Y), under the control of the tetO, tetracycline-responsive regulatory element. In the absence of tetracycline or a tetracycline analog (such as doxycycline), selective expression of beta-galactosidase is observed in activated neurons. Doxycycline administration prevents expression beta-galactosidase in neurons. However, neurons that were induced to express beta-galactosidase during the absence of doxycycline administration will continue to express beta-galactosidase via the doxycycline insensitive mutant tTAH100Y. Mice hemizygous for each transgene are viable and fertile. The strain is maintained with doxycycline adminstration to prevent neuronal beta-galactosidase expression. The donating investigator has not attempted to make these mice homozygous for the transgenes. This strain provides a tool that allows the inducible stable labeling of activated neurons.Of note, cfos-htTA mice (cfos-tTA/cfos-shEGFP) harboring only the co-injected cfos-tTA/cfos-shEGFP transgenes are available at The Jackson Laboratory as Stock No. 018306.
Development
To produce this bi-transgenic line, two independently generated single transgenic lines were crossed.The first transgenic line was generated by co-injection of a construct containing the tetracycline regulated transactivator (tTA), SV40 polyadenylation signal sequence and the fos, FBJ osteosarcoma oncogene, minimal promoter and a second construct containing a two hour half-life Green Fluorescent Protein under the control of the fos, FBJ osteosarcoma oncogene, minimal promoter, into fertilized B6D2F2 mouse eggs. These co-injected transgenes segregated as a single transgene.
The second transgenic line was generated using a construct including sequence encoding tau-LacZ and the doxycycline insensitive tetracycline regulated transactivator (containing point mutation, H100Y), under the control of the tetO, tetracycline-responsive regulatory element. This construct was injected into fertilized B6D2F2 mouse eggs.
The two independent transgenic lines were then crossed to produce this bi-transgenic strain. The mice were then backcrossed to C57BL/6J for 3 generations.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 018306 B6.Cg-Tg(Fos-tTA,Fos-EGFP*)1Mmay/J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (357 strains)
018306 B6.Cg-Tg(Fos-tTA,Fos-EGFP*)1Mmay/J
006100 B10.Cg-H2k Tg(NFkB/Fos-luc)26Rinc/J 002099 B6.129X1-Fostm1Pa/J 014135 B6.Cg-Tg(Fos/EGFP)1-3Brth/J 002293 B6;129X1-Fostm1Pa/J 016236 STOCK Tg(TCF/Lef1-cre/ERT2)1Dje/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Tet Expression Systems
Use of MICE by companies or for-profit entities requires a license prior to shipping.
Use of MICE by companies or for-profit entities requires a license prior to shipping.
Phenotype
Phenotype Information
assigned by genotype
Tg(Fos-tTA,Fos-EGFP*)1Mmay/0 Tg(tetO-lacZ,tTA*)1Mmay/0
involves: C57BL/6 * DBA/2
- no phenotypic analysis
- *normal* no phenotypic analysis
- transgenic mice are used only for lineage tracing; expression data, but no phenotypic analysis are provided (MGI Ref ID J:125221)
This mouse can be used to support research in many areas including:
GFP relatedNeurobiology Research
lacZ expression in neural tissue
Fluorescent protein expression in neural tissue
Tet Expression System
tTA/rtTA Expressing Strains
tTA/rtTA Responsive Strains
Research Tools
lacZ Expression
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers
Tissue/Cell Markers: neurons
Neurobiology Research
Tetop Tet System
cell marker
Tet Expression Systems
tTA/rtTA Expressing Strains
tTA/rtTA Responsive Strains
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Fos-tTA,Fos-EGFP*)1Mmay | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Mark Mayford | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | cfos-htTA mice; | ||
| Strain of Origin | (C57BL/6 x DBA/2)F2 | ||
| Site of Expression | activated neurons in the basolateral amygdala | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Expressed Gene | tTA, tetracycline-controlled transactivator, E. coli | ||
| The tetracycline-resistance gene (TetR), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). TetR was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). The tetracycline-inhibitable transcription factor is a component of a bigenic system that allows doxycycline (a tetracycline analog) regulatable expression of genes that are under the direction of the tetracycline responsive promoter (TetOp)promoter. | |||
| Promoter | Fos, FBJ osteosarcoma oncogene, mouse, laboratory | ||
| Molecular Note | A construct containing the Fos (FBJ osteosarcoma oncogene, minimal promoter) driving the tetracycline regulated transactivator (tTA) sequence, the SV40 polyadenylation signal sequence and a second construct containing a two hour half-life Green Fluorescent Protein (shEGFP) under the control of the Fos minimal promoter, were co-injected into fertilized B6D2F2 mouse eggs. These co-injected transgenes segregate as a single transgene. [MGI Ref ID J:125221] | ||
| Allele Symbol | Tg(tetO-lacZ,tTA*)1Mmay | ||
| Allele Name | transgene insertion 1, Mark Mayford | ||
| Allele Type | Transgenic (Reporter) | ||
| Strain of Origin | (C57BL/6 x DBA/2)F2 | ||
| Site of Expression | activated neurons in the basolateral amygdala | ||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Expressed Gene | tTA, tetracycline-controlled transactivator, E. coli | ||
| The tetracycline-resistance gene (TetR), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). TetR was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). The tetracycline-inhibitable transcription factor is a component of a bigenic system that allows doxycycline (a tetracycline analog) regulatable expression of genes that are under the direction of the tetracycline responsive promoter (TetOp)promoter. | |||
| Promoter | tetO, tet operator, | ||
| Molecular Note | A construct including the sequence encoding tau-LacZ and a doxycycline-insensitive tetracycline regulated transactivator (containing point mutation, H100Y), under the control of a bi-cistronic tetO promoter (tetracycline-responsive regulatory element) was injected into fertilized eggs. [MGI Ref ID J:125221] | ||
| Gene Symbol and Name | Tg(tetO-lacZ,tTA*)1Mmay, transgene insertion 1, Mark Mayford | ||
| Chromosome | UN | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Melt Curve Analysis
Fluorescent Proteins (Generic GFP), Standard PCR
Generic LacZ Melt Curve Analysis, Melt Curve Analysis
Generic LacZ, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Reijmers LG; Perkins BL; Matsuo N; Mayford M. 2007. Localization of a stable neural correlate of associative memory. Science 317(5842):1230-3. [PubMed: 17761885] [MGI Ref ID J:125221]
Additional References
Tg(Fos-tTA,Fos-EGFP*)1Mmay relatedGarner AR; Rowland DC; Hwang SY; Baumgaertel K; Roth BL; Kentros C; Mayford M. 2012. Generation of a synthetic memory trace. Science 335(6075):1513-6. [PubMed: 22442487] [MGI Ref ID J:181832]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are bred hemizygous for each transgene. The strain is maintained with doxycycline adminstration to prevent neuronal beta-galactosidase expression. The donating investigator notes that his lab has not been successful in their attempts to generate homozygotes from this line. Mating System See Colony Maintenance under the Health & husbandry tab (Female x Male) 06-FEB-09 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Hemizygous for Tg(Fos-tTA,Fos-EGFP*)1Mmay, Hemizygous for Tg(tetO-lacZ,tTA*)1Mmay $232.00 Female or Male Hemizygous for Tg(Fos-tTA,Fos-EGFP*)1Mmay, Noncarrier Individual Mouse $232.00 Female or Male Noncarrier, Hemizygous for Tg(tetO-lacZ,tTA*)1Mmay
Price per Pair (US dollars $) Pair Genotype $296.00 Hemizygous for Tg(Fos-tTA,Fos-EGFP*)1Mmay, Hemizygous for Tg(tetO-lacZ,tTA*)1Mmay x Noncarrier, Noncarrier $296.00 Hemizygous for Tg(Fos-tTA,Fos-EGFP*)1Mmay, Noncarrier x Noncarrier, Hemizygous for Tg(tetO-lacZ,tTA*)1Mmay $296.00 Noncarrier, Hemizygous for Tg(tetO-lacZ,tTA*)1Mmay x Hemizygous for Tg(Fos-tTA,Fos-EGFP*)1Mmay, Noncarrier $296.00 Noncarrier, Noncarrier x Hemizygous for Tg(Fos-tTA,Fos-EGFP*)1Mmay, Hemizygous for Tg(tetO-lacZ,tTA*)1Mmay Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Hemizygous for Tg(Fos-tTA,Fos-EGFP*)1Mmay, Hemizygous for Tg(tetO-lacZ,tTA*)1Mmay $301.60 Female or Male Hemizygous for Tg(Fos-tTA,Fos-EGFP*)1Mmay, Noncarrier Individual Mouse $301.60 Female or Male Noncarrier, Hemizygous for Tg(tetO-lacZ,tTA*)1Mmay
Price per Pair (US dollars $) Pair Genotype $384.80 Hemizygous for Tg(Fos-tTA,Fos-EGFP*)1Mmay, Hemizygous for Tg(tetO-lacZ,tTA*)1Mmay x Noncarrier, Noncarrier $384.80 Hemizygous for Tg(Fos-tTA,Fos-EGFP*)1Mmay, Noncarrier x Noncarrier, Hemizygous for Tg(tetO-lacZ,tTA*)1Mmay $384.80 Noncarrier, Hemizygous for Tg(tetO-lacZ,tTA*)1Mmay x Hemizygous for Tg(Fos-tTA,Fos-EGFP*)1Mmay, Noncarrier $384.80 Noncarrier, Noncarrier x Hemizygous for Tg(Fos-tTA,Fos-EGFP*)1Mmay, Hemizygous for Tg(tetO-lacZ,tTA*)1Mmay Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 018306 B6.Cg-Tg(Fos-tTA,Fos-EGFP*)1Mmay/J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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