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| These targeted mutation mice carry a tamoxifen-inducible Cre cassette knocked into the 3' UTR of the Chat, choline acetyltransferase gene. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating 4-hydroxytamoxifen-induced, Cre-mediated targeted deletions specifically in cholinergic neurons. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6;129-Chattm1(Cre/Esr1)Nat/J (Changed: 02-SEP-09 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote (Female x Male) 06-JAN-09 Species laboratory mouse Generation F?+F1 (14-JAN-09) Donating Investigator Jeremy Nathans, Johns Hopkins University Description
These targeted mutation mice carry a tamoxifen-inducible Cre cassette knocked into the 3' UTR of the gene. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating 4-hydroxytamoxifen-induced, Cre-mediated targeted deletions specifically in cholinergic neurons. Heterozygotes and homozygotes are normal in size, viability and fertility.Development
An IRES-creER cassette followed by an Frt-PGK-Neo-Frt selectable marker was knocked in to the 3' UTR of the gene using a 129-derived JHMI-ES1 embryonic stem cell line. The PGK-Neo was removed by crossing to a C57BL/6 background Flp recombinase strain. Flp was then bred out of the line. This strain was maintained on a mixed C57BL/6 and 129 background by the donating laboratory.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Chat
007902 B6.Cg-Tg(RP23-268L19-EGFP)2Mik/J 006410 B6;129S6-Chattm1(cre)Lowl/J View Strains carrying other alleles of Chat (2 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
Chattm1(cre/Esr1)Nat/Chattm1(cre/Esr1)Nat
involves: 129 * C57BL/6
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:144315)
This mouse can be used to support research in many areas including:
Neurobiology Research
Alzheimer's Disease
Cre-lox System
Cre Recombinase expression in neural tissue
Parkinson's Disease
strains expressing cre
Research Tools
Cre-lox System
Cre Recombinase Expression: Inducible
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Chattm1(cre/Esr1)Nat | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Jeremy Nathans | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | ChAT-IRES-creER(T); | ||
| Strain of Origin | 129 | ||
| ES Cell Line Name | Other (see notes) | ||
| ES Cell Line Strain | 129 | ||
| Site of Expression | cholinergic neurons (after induction) | ||
| Gene Symbol and Name | Chat, choline acetyltransferase | ||
| Chromosome | 14 | ||
| Gene Common Name(s) | B230380D24Rik; CMS1A; CMS1A2; RIKEN cDNA B230380D24 gene; | ||
| Driver Note | Chat | ||
| General Note | ES cell line = JHMI-ES1. | ||
| Inducible Note | Induced by tamoxifen. | ||
| Molecular Note | An IRES-creER cassette, a tetracycline resistance gene, and a Frt-flanked neomycin selection marker were all knocked in to the 3' UTR of the gene. The neomycin selection marker was removed by crossing to a C57BL/6 background Flp recombinase strain. Flp was then bred out of the line. When crossed with a Tg(CAG-Bgeo/ALPP)1Lbe reporter strain and given tamoxifen, cre expression was detected in amacrine cells of the retina, and in a sparse number of cholinergic neurons in the brain. [MGI Ref ID J:144315] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Chattm1(cre/Esr1), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Rotolo T; Smallwood PM; Williams J; Nathans J. 2008. Genetically-directed, cell type-specific sparse labeling for the analysis of neuronal morphology. PLoS ONE 3(12):e4099. [PubMed: 19116659] [MGI Ref ID J:144315]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintained as a live colony, heterozygotes or homozygotes may be bred. Mating System Homozygote x Homozygote (Female x Male) 06-JAN-09 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $160.40 Female or Male Homozygous for Chattm1(Cre/Esr1)Nat
Pairs /Price (US dollars $) Pair Genotype $320.80 Homozygous for Chattm1(Cre/Esr1)Nat x Homozygous for Chattm1(Cre/Esr1)Nat
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $208.60 Female or Male Homozygous for Chattm1(Cre/Esr1)Nat
Pairs /Price (US dollars $) Pair Genotype $417.10 Homozygous for Chattm1(Cre/Esr1)Nat x Homozygous for Chattm1(Cre/Esr1)Nat
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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| fax: | 207-288-6655 |
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