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| This reporter strain may be used to detect and monitor cells committed to interleukin 10 (IL10) production. Green fluorescent protein knocked into the gene faithfully recapitulates normal expression patterns but is preferentially observed in some populations of the intestinal tissue. Expression assayed by fluorescent-activated cell sorting (FACS) has been detected after activation in macrophages and dendritic cells as well as intraintestinal lymphocytes and lamina propria lymphocytes. Targeted allele expression is slightly reduced, as compared to wild-type. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System +/+ sibling x Heterozygote (Female x Male) 19-DEC-08 Species laboratory mouse Generation N11+N1F2 (18-NOV-09) Donating Investigator Richard Flavell, Yale University School of Medicine Description
This reporter strain may be used to detect and monitor cells committed to interleukin 10 production. Green fluorescent protein knocked into the gene faithfully recapitulates normal expression patterns but is preferentially observed in some populations of the intestinal tissue. Expression assayed by fluorescent-activated cell sorting (FACS) has been detected after activation in macrophages and dendritic cells as well as intraintestinal lymphocytes and lamina propria lymphocytes. Targeted allele expression is slightly reduced, as compared to wild-type. Although homozygotes have reduced IL10 expression, they are both viable and fertile.Development
An IRES-GFP (internal ribosome entry site-green fluorescent protein) cassette followed by a loxP-flanked neomycin resistance cassette was inserted between the stop codon and polyadenylation signal of exon 5 of the gene. The targeting vector was electroporated into 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells. Selected clones were transfected with Cre-recombinase to delete the neomycin resistance cassette. Targeted clones were injected into C57BL/6 blastocysts. Chimeric males showing germline transmission were bred to C57BL/6 mice. This line was backcrossed to C57BL/6Ncr for ten generations by the donating investigator.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
004368 129(B6)-Il10tm1Cgn/J 002250 B10.129P2(B6)-Il10tm1Cgn/J 002251 B6.129P2-Il10tm1Cgn/J 005912 B6.Cg-Il10tm1Cgn (D3Mit49-D3Mit348)/Lt 004333 C.129P2(B6)-Il10tm1Cgn/J 005973 C3Bir.129P2(B6)-Il10C3Bir/LtJ 004326 C3Bir.129P2(B6)-Il10tm1Cgn/Lt 003968 C3Bir.129P2(B6)-Il10tm1Cgn/LtJ 005346 NOD.Cg-Il10tm1Cgn Casp1tm1Sesh/LtJ 004291 NOD.Cg-Il10tm1Cgn Il4tm1Cgn/DvsJ 004266 NOD.Cg-Il10tm1Cgn/DvsJ
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Il10tm1Flv/Il10tm1Flv
involves: 129S6/SvEvTac * C57BL/6
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:116616)
- viable and fertile with no signs of inflammatory bowel disease
This mouse can be used to support research in many areas including:
Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
Research Tools
Fluorescent Proteins
Immunology and Inflammation Research
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Il10tm1Flv | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Richard A Flavell | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | IL-10-GFP tiger; tiger; | ||
| Mutation Made By | Richard Flavell, Yale University School of Medicine | ||
| Strain of Origin | 129S6/SvEvTac | ||
| ES Cell Line Name | TC-1 | ||
| ES Cell Line Strain | 129S6/SvEvTac | ||
| Gene Symbol and Name | Il10, interleukin 10 | ||
| Chromosome | 1 | ||
| Gene Common Name(s) | CSIF; IL-10; IL10A; IL10X; Il-10; MGC126450; MGC126451; TGIF; cytokine synthesis inhibitory factor; | ||
| Molecular Note | A targeting vector containing IRES-GFP was inserted into the locus between the stop codon and the polyA signal in exon 5. A floxed neo was removed via cre mediated recombination. [MGI Ref ID J:116616] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Il10tm1Flv, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Kamanaka M; Kim ST; Wan YY; Sutterwala FS; Lara-Tejero M; Galan JE; Harhaj E; Flavell RA. 2006. Expression of interleukin-10 in intestinal lymphocytes detected by an interleukin-10 reporter knockin tiger mouse. Immunity 25(6):941-52. [PubMed: 17137799] [MGI Ref ID J:116616]
Additional References
Il10tm1Flv relatedAwasthi A; Carrier Y; Peron JP; Bettelli E; Kamanaka M; Flavell RA; Kuchroo VK; Oukka M; Weiner HL. 2007. A dominant function for interleukin 27 in generating interleukin 10-producing anti-inflammatory T cells. Nat Immunol 8(12):1380-9. [PubMed: 17994022] [MGI Ref ID J:127774]
Dardalhon V; Awasthi A; Kwon H; Galileos G; Gao W; Sobel RA; Mitsdoerffer M; Strom TB; Elyaman W; Ho IC; Khoury S; Oukka M; Kuchroo VK. 2008. IL-4 inhibits TGF-beta-induced Foxp3+ T cells and, together with TGF-beta, generates IL-9+ IL-10+ Foxp3(-) effector T cells. Nat Immunol 9(12):1347-55. [PubMed: 18997793] [MGI Ref ID J:143168]
Jankovic D; Kullberg MC; Feng CG; Goldszmid RS; Collazo CM; Wilson M; Wynn TA; Kamanaka M; Flavell RA; Sher A. 2007. Conventional T-bet(+)Foxp3(-) Th1 cells are the major source of host-protective regulatory IL-10 during intracellular protozoan infection. J Exp Med 204(2):273-83. [PubMed: 17283209] [MGI Ref ID J:125371]
Wilson MS; Elnekave E; Mentink-Kane MM; Hodges MG; Pesce JT; Ramalingam TR; Thompson RW; Kamanaka M; Flavell RA; Keane-Myers A; Cheever AW; Wynn TA. 2007. IL-13Ralpha2 and IL-10 coordinately suppress airway inflammation, airway-hyperreactivity, and fibrosis in mice. J Clin Invest 117(10):2941-51. [PubMed: 17885690] [MGI Ref ID J:127527]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintained as a live colony, heterozygotes or homozygotes may be bred. The donating laboratory maintained the line heterozygously; homozygotes were not used experimentally due to decreased IL10 production. Mating System +/+ sibling x Heterozygote (Female x Male) 19-DEC-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Heterozygous for Il10tm1Flv
Pairs /Price (US dollars $) Pair Genotype $297.85 Heterozygous for Il10tm1Flv x Wild-type for Il10tm1Flv $297.85 Wild-type for Il10tm1Flv x Heterozygous for Il10tm1Flv
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Heterozygous for Il10tm1Flv
Pairs /Price (US dollars $) Pair Genotype $387.30 Heterozygous for Il10tm1Flv x Wild-type for Il10tm1Flv $387.30 Wild-type for Il10tm1Flv x Heterozygous for Il10tm1Flv
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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