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| This reporter strain may be used to detect and monitor cells committed to interleukin 10 (IL10) production. Green fluorescent protein knocked into the gene faithfully recapitulates normal expression patterns but is preferentially observed in some populations of the intestinal tissue. Expression assayed by fluorescent-activated cell sorting (FACS) has been detected after activation in macrophages and dendritic cells as well as intraintestinal lymphocytes and lamina propria lymphocytes. Targeted allele expression is slightly reduced, as compared to wild-type. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
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Description
Strain Information
Type Congenic; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System +/+ sibling x Heterozygote (Female x Male) 19-DEC-08 Species laboratory mouse Generation N11+N1F9 (03-DEC-12)
Generation DefinitionsDonating Investigator Dr. Richard A. Flavell, Yale University School of Medicine Description
This reporter strain may be used to detect and monitor cells committed to interleukin 10 production. Green fluorescent protein knocked into the gene faithfully recapitulates normal expression patterns but is preferentially observed in some populations of the intestinal tissue. Expression assayed by fluorescent-activated cell sorting (FACS) has been detected after activation in macrophages and dendritic cells as well as intraintestinal lymphocytes and lamina propria lymphocytes. Targeted allele expression is slightly reduced, as compared to wild-type. Although homozygotes have reduced IL10 expression, they are both viable and fertile.Development
An IRES-GFP (internal ribosome entry site-green fluorescent protein) cassette followed by a loxP-flanked neomycin resistance cassette was inserted between the stop codon and polyadenylation signal of exon 5 of the gene. The targeting vector was electroporated into 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells. Selected clones were transfected with Cre-recombinase to delete the neomycin resistance cassette. Targeted clones were injected into C57BL/6 blastocysts. Chimeric males showing germline transmission were bred to C57BL/6 mice. This line was backcrossed to C57BL/6Ncr for ten generations by the donating investigator.SNP (single nucleotide polymorphism) analysis performed by The Jackson Laboratory revealed that the Donating Investigator used C57BL/6N (4 of 5 markers segregating) in the development of the strain. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J at least once to establish the colony.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (357 strains)
004368 129(B6)-Il10tm1Cgn/J 002250 B10.129P2(B6)-Il10tm1Cgn/J 014530 B6(Cg)-Il10tm1.1Karp/J 002251 B6.129P2-Il10tm1Cgn/J 005912 B6.Cg-Il10tm1Cgn (D3Mit49-D3Mit348)/Lt 004333 C.129P2(B6)-Il10tm1Cgn/J 005973 C3Bir.129P2(B6)-Il10C3Bir/LtJ 004326 C3Bir.129P2(B6)-Il10tm1Cgn/Lt 003968 C3Bir.129P2(B6)-Il10tm1Cgn/LtJ 005346 NOD.Cg-Il10tm1Cgn Casp1tm1Sesh Casp4del/LtJ 004291 NOD.Cg-Il10tm1Cgn Il4tm1Cgn/DvsJ 004266 NOD.Cg-Il10tm1Cgn/DvsJ
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Graft-Versus-Host Disease, Susceptibility To; GVHDS (IL10)
Human Immunodeficiency Virus Type 1, Susceptibility to (IL10)
Rheumatoid Arthritis; RA (IL10)
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Il10tm1Flv/Il10tm1Flv
involves: 129S6/SvEvTac * C57BL/6
- normal phenotype
- no abnormal phenotype detected
- viable and fertile with no signs of inflammatory bowel disease (MGI Ref ID J:116616)
This mouse can be used to support research in many areas including:
GFP relatedImmunology, Inflammation and Autoimmunity Research
Growth Factors/Receptors/Cytokines
Research Tools
Fluorescent Proteins
Immunology and Inflammation Research
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Il10tm1Flv | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Richard A Flavell | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | IL-10-GFP tiger; tiger; | ||
| Mutation Made By | Dr. Richard Flavell, Yale University School of Medicine | ||
| Strain of Origin | 129S6/SvEvTac | ||
| ES Cell Line Name | TC1/TC-1 | ||
| ES Cell Line Strain | 129S6/SvEvTac | ||
| Site of Expression | GFP expression recapitulates normal Il10 expression patterns (including activated macrophages and dendritic cells, and some lymphocytes) but is preferentially observed in some populations of the intestinal tissue. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Molecular Note | A targeting vector containing IRES-GFP was inserted into the locus between the stop codon and the polyA signal in exon 5. A floxed neo was removed via cre mediated recombination. [MGI Ref ID J:116616] | ||
| Gene Symbol and Name | Il10, interleukin 10 | ||
| Chromosome | 1 | ||
| Gene Common Name(s) | CSIF; GVHDS; IL-10; IL10A; IL10X; Il-10; TGIF; cytokine synthesis inhibitory factor; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Il10 tm1Flv, Melt Curve Analysis
Il10tm1Flv, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Kamanaka M; Kim ST; Wan YY; Sutterwala FS; Lara-Tejero M; Galan JE; Harhaj E; Flavell RA. 2006. Expression of interleukin-10 in intestinal lymphocytes detected by an interleukin-10 reporter knockin tiger mouse. Immunity 25(6):941-52. [PubMed: 17137799] [MGI Ref ID J:116616]
Additional References
Il10tm1Flv relatedAwasthi A; Carrier Y; Peron JP; Bettelli E; Kamanaka M; Flavell RA; Kuchroo VK; Oukka M; Weiner HL. 2007. A dominant function for interleukin 27 in generating interleukin 10-producing anti-inflammatory T cells. Nat Immunol 8(12):1380-9. [PubMed: 17994022] [MGI Ref ID J:127774]
Bai F; Town T; Qian F; Wang P; Kamanaka M; Connolly TM; Gate D; Montgomery RR; Flavell RA; Fikrig E. 2009. IL-10 signaling blockade controls murine West Nile virus infection. PLoS Pathog 5(10):e1000610. [PubMed: 19816558] [MGI Ref ID J:162907]
Bollyky PL; Wu RP; Falk BA; Lord JD; Long SA; Preisinger A; Teng B; Holt GE; Standifer NE; Braun KR; Xie CF; Samuels PL; Vernon RB; Gebe JA; Wight TN; Nepom GT. 2011. ECM components guide IL-10 producing regulatory T-cell (TR1) induction from effector memory T-cell precursors. Proc Natl Acad Sci U S A 108(19):7938-43. [PubMed: 21518860] [MGI Ref ID J:172198]
Collison LW; Chaturvedi V; Henderson AL; Giacomin PR; Guy C; Bankoti J; Finkelstein D; Forbes K; Workman CJ; Brown SA; Rehg JE; Jones ML; Ni HT; Artis D; Turk MJ; Vignali DA. 2010. IL-35-mediated induction of a potent regulatory T cell population. Nat Immunol 11(12):1093-101. [PubMed: 20953201] [MGI Ref ID J:167328]
Cook PC; Jones LH; Jenkins SJ; Wynn TA; Allen JE; Macdonald AS. 2012. Alternatively activated dendritic cells regulate CD4+ T-cell polarization in vitro and in vivo. Proc Natl Acad Sci U S A 109(25):9977-82. [PubMed: 22660926] [MGI Ref ID J:185513]
Dardalhon V; Awasthi A; Kwon H; Galileos G; Gao W; Sobel RA; Mitsdoerffer M; Strom TB; Elyaman W; Ho IC; Khoury S; Oukka M; Kuchroo VK. 2008. IL-4 inhibits TGF-beta-induced Foxp3+ T cells and, together with TGF-beta, generates IL-9+ IL-10+ Foxp3(-) effector T cells. Nat Immunol 9(12):1347-55. [PubMed: 18997793] [MGI Ref ID J:143168]
Esplugues E; Huber S; Gagliani N; Hauser AE; Town T; Wan YY; O'Connor W Jr; Rongvaux A; Van Rooijen N; Haberman AM; Iwakura Y; Kuchroo VK; Kolls JK; Bluestone JA; Herold KC; Flavell RA. 2011. Control of TH17 cells occurs in the small intestine. Nature 475(7357):514-8. [PubMed: 21765430] [MGI Ref ID J:174687]
Gaddi PJ; Crane MJ; Kamanaka M; Flavell RA; Yap GS; Salazar-Mather TP. 2012. IL-10 mediated regulation of liver inflammation during acute murine cytomegalovirus infection. PLoS One 7(8):e42850. [PubMed: 22880122] [MGI Ref ID J:189817]
Gitlin JM; Trivedi DB; Langenbach R; Loftin CD. 2007. Genetic deficiency of cyclooxygenase-2 attenuates abdominal aortic aneurysm formation in mice. Cardiovasc Res 73(1):227-36. [PubMed: 17137566] [MGI Ref ID J:162736]
Horikawa M; Weimer ET; DiLillo DJ; Venturi GM; Spolski R; Leonard WJ; Heise MT; Tedder TF. 2013. Regulatory B cell (B10 Cell) expansion during Listeria infection governs innate and cellular immune responses in mice. J Immunol 190(3):1158-68. [PubMed: 23275601] [MGI Ref ID J:193035]
Huber S; Gagliani N; Esplugues E; O'Connor W Jr; Huber FJ; Chaudhry A; Kamanaka M; Kobayashi Y; Booth CJ; Rudensky AY; Roncarolo MG; Battaglia M; Flavell RA. 2011. Th17 Cells Express Interleukin-10 Receptor and Are Controlled by Foxp3(-) and Foxp3(+) Regulatory CD4(+) T Cells in an Interleukin-10-Dependent Manner. Immunity 34(4):554-65. [PubMed: 21511184] [MGI Ref ID J:171598]
Jankovic D; Kullberg MC; Feng CG; Goldszmid RS; Collazo CM; Wilson M; Wynn TA; Kamanaka M; Flavell RA; Sher A. 2007. Conventional T-bet(+)Foxp3(-) Th1 cells are the major source of host-protective regulatory IL-10 during intracellular protozoan infection. J Exp Med 204(2):273-83. [PubMed: 17283209] [MGI Ref ID J:125371]
Mandaric S; Walton SM; Rulicke T; Richter K; Girard-Madoux MJ; Clausen BE; Zurunic A; Kamanaka M; Flavell RA; Jonjic S; Oxenius A. 2012. IL-10 suppression of NK/DC crosstalk leads to poor priming of MCMV-specific CD4 T cells and prolonged MCMV persistence. PLoS Pathog 8(8):e1002846. [PubMed: 22876184] [MGI Ref ID J:195373]
Maseda D; Smith SH; DiLillo DJ; Bryant JM; Candando KM; Weaver CT; Tedder TF. 2012. Regulatory B10 cells differentiate into antibody-secreting cells after transient IL-10 production in vivo. J Immunol 188(3):1036-48. [PubMed: 22198952] [MGI Ref ID J:180761]
Miles K; Heaney J; Sibinska Z; Salter D; Savill J; Gray D; Gray M. 2011. A tolerogenic role for Toll-like receptor 9 is revealed by B-cell interaction with DNA complexes expressed on apoptotic cells. Proc Natl Acad Sci U S A :. [PubMed: 22207622] [MGI Ref ID J:179971]
Ranatunga DC; Ramakrishnan A; Uprety P; Wang F; Zhang H; Margolick JB; Brayton C; Bream JH. 2012. A protective role for human IL-10-expressing CD4+ T cells in colitis. J Immunol 189(3):1243-52. [PubMed: 22753934] [MGI Ref ID J:189774]
Sayi A; Kohler E; Toller IM; Flavell RA; Muller W; Roers A; Muller A. 2011. TLR-2-activated B cells suppress Helicobacter-induced preneoplastic gastric immunopathology by inducing T regulatory-1 cells. J Immunol 186(2):878-90. [PubMed: 21149607] [MGI Ref ID J:168773]
Sonderegger FL; Ma Y; Maylor-Hagan H; Brewster J; Huang X; Spangrude GJ; Zachary JF; Weis JH; Weis JJ. 2012. Localized production of IL-10 suppresses early inflammatory cell infiltration and subsequent development of IFN-gamma-mediated Lyme arthritis. J Immunol 188(3):1381-93. [PubMed: 22180617] [MGI Ref ID J:180776]
Tessmer MS; Reilly EC; Brossay L. 2011. Salivary gland NK cells are phenotypically and functionally unique. PLoS Pathog 7(1):e1001254. [PubMed: 21249177] [MGI Ref ID J:172475]
Wang H; Shin DM; Abbasi S; Jain S; Kovalchuk AL; Beaty N; Chen S; Gonzalez-Garcia I; Morse HC 3rd. 2012. Expression of plasma cell alloantigen 1 defines layered development of B-1a B-cell subsets with distinct innate-like functions. Proc Natl Acad Sci U S A 109(49):20077-82. [PubMed: 23169635] [MGI Ref ID J:192331]
Wilson MS; Elnekave E; Mentink-Kane MM; Hodges MG; Pesce JT; Ramalingam TR; Thompson RW; Kamanaka M; Flavell RA; Keane-Myers A; Cheever AW; Wynn TA. 2007. IL-13Ralpha2 and IL-10 coordinately suppress airway inflammation, airway-hyperreactivity, and fibrosis in mice. J Clin Invest 117(10):2941-51. [PubMed: 17885690] [MGI Ref ID J:127527]
Wilson MS; Madala SK; Ramalingam TR; Gochuico BR; Rosas IO; Cheever AW; Wynn TA. 2010. Bleomycin and IL-1beta-mediated pulmonary fibrosis is IL-17A dependent. J Exp Med 207(3):535-52. [PubMed: 20176803] [MGI Ref ID J:158818]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintained as a live colony, heterozygotes or homozygotes may be bred. The donating laboratory maintained the line heterozygously; homozygotes were not used experimentally due to decreased IL10 production. Mating System +/+ sibling x Heterozygote (Female x Male) 19-DEC-08 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Heterozygous for Il10tm1Flv
Price per Pair (US dollars $) Pair Genotype $296.00 Heterozygous for Il10tm1Flv x Wild-type for Il10tm1Flv $296.00 Wild-type for Il10tm1Flv x Heterozygous for Il10tm1Flv Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Heterozygous for Il10tm1Flv
Price per Pair (US dollars $) Pair Genotype $384.80 Heterozygous for Il10tm1Flv x Wild-type for Il10tm1Flv $384.80 Wild-type for Il10tm1Flv x Heterozygous for Il10tm1Flv Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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|
|
Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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