Description

Strain Information

Type Coisogenic; Transgenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse   Donating Investigator Thomas Sudhof,   Stanford University School of Medicine

Description
This transgenic strain carries a human THY1 promoter driving expression of the human synuclein, alpha (SNCA) gene. Levels of expression show a 5-fold increase in the brain and a 10-fold increase in the spinal cord. Hemizygotes are viable and fertile and unlike some similar mutant transgenic lines, do not display any Parkinson-like phenotype upon aging.

Development
A human THY1 promoter drives expression of the human SNCA gene. This strain was constructed and maintained on a C57BL/6 background.

Related Strains

Strains carrying other alleles of SNCA

008473	B6.Cg-Tg(THY1-SNCA*A30P)M30Sud/J
008134	B6.Cg-Tg(THY1-SNCA*A30P)TS2Sud/J
008474	B6.Cg-Tg(THY1-SNCA*A53T)F53Sud/J
008135	B6.Cg-Tg(THY1-SNCA*A53T)M53Sud/J
004479	B6;C3-Tg(Prnp-SNCA*A53T)83Vle/J

View Strains carrying other alleles of SNCA     (5 strains)

Strains carrying other alleles of THY1

008473	B6.Cg-Tg(THY1-SNCA*A30P)M30Sud/J
008134	B6.Cg-Tg(THY1-SNCA*A30P)TS2Sud/J
008474	B6.Cg-Tg(THY1-SNCA*A53T)F53Sud/J
008135	B6.Cg-Tg(THY1-SNCA*A53T)M53Sud/J
008132	STOCK Tg(THY1-Snca)M1mSud/J

View Strains carrying other alleles of THY1     (5 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Tg(THY1-SNCA)1Sud/0

        involves: C57BL/6

• nervous system phenotype
• decreased motor neuron number (MGI Ref ID J:115193)
  • at 12-16 months, mice have only ~40% of motor neuron numbers in non-transgenic mice
• motor neuron degeneration (MGI Ref ID J:115193)
  • mice show onset of motoneuron disease at ~5-6 months

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Neurobiology Research
Parkinson's Disease (synuclein mutants)

Genes & Alleles

Gene & Allele Information

Allele Symbol		Tg(THY1-SNCA)1Sud
Allele Name		transgene insertion 1, Thomas C Sudhof
Allele Type		Transgenic (random, expressed)
Common Name(s)		LHS; Synhtg;
Mutation Made By		Thomas Sudhof,   Stanford University School of Medicine
Expressed Gene		SNCA, synuclein, alpha (non A4 component of amyloid precursor), human
Promoter		THY1, Thy-1 cell surface antigen, human
Molecular Note		The transgene expresses the human SNCA (synuclein, alpha) gene under the control of a human THY1 (thymus cell antigen 1, theta) promoter. [MGI Ref ID J:115193]

Genotyping

Genotyping Information

Genotyping Protocols

Tg(THY1-SNCA)1Sud, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Chandra S; Gallardo G; Fernandez-Chacon R; Schluter OM; Sudhof TC. 2005. Alpha-synuclein cooperates with CSPalpha in preventing neurodegeneration. Cell 123(3):383-96. [PubMed: 16269331]  [MGI Ref ID J:115193]

Additional References

Tg(THY1-SNCA)1Sud related

Gallardo G; Schluter OM; Sudhof TC. 2008. A molecular pathway of neurodegeneration linking alpha-synuclein to ApoE and Abeta peptides. Nat Neurosci 11(3):301-8. [PubMed: 18297066]  [MGI Ref ID J:131733]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	When maintained as a live colony, hemizygotes may be bred. It is not known whether homozygotes are viable or fertile.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

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In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.